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Search for "fetal calf serum" in Full Text gives 29 result(s) in Beilstein Journal of Nanotechnology.

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

  • Dagmar A. Kuhn,
  • Dimitri Vanhecke,
  • Benjamin Michen,
  • Fabian Blank,
  • Peter Gehr,
  • Alke Petri-Fink and
  • Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

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  • coverglass system, NC-155382, Nunc, Milian, Geneva, Switzerland), stained with Cell trackerTM violet BMQC dye and incubated for 1 hour at 37 °C and 5% CO2 followed by three washing steps with 1× PBS. Finally, transparent RPMI 1640 medium (no 1% L-glutamine, no antibiotics, no fetal calf serum and without
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Published 24 Sep 2014

Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure

  • Alicja Panas,
  • Andreas Comouth,
  • Harald Saathoff,
  • Thomas Leisner,
  • Marco Al-Rawi,
  • Michael Simon,
  • Gunnar Seemann,
  • Olaf Dössel,
  • Sonja Mülhopt,
  • Hanns-Rudolf Paur,
  • Susanne Fritsch-Decker,
  • Carsten Weiss and
  • Silvia Diabaté

Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171

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  • Life Technologies (Frankfurt am Main, Germany). Fetal calf serum (FCS) was from PAA (Cölbe, Germany). The cytotoxicity detection kit for determining release of LDH was from Roche (Mannheim, Germany). Enzyme-linked immuno assays (ELISA) for the detection of human IL-6 and IL-8 were from BD Biosciences
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Published 19 Sep 2014

The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles

  • Dominic Docter,
  • Christoph Bantz,
  • Dana Westmeier,
  • Hajo J. Galla,
  • Qiangbin Wang,
  • James C. Kirkpatrick,
  • Peter Nielsen,
  • Michael Maskos and
  • Roland H. Stauber

Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151

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  • % fetal calf serum (FCS). According to dynamic light scattering (DLS) and zeta potential measurements, all ASP display the expected hydrodynamic diameter and carry negative surface charges in water, as reflected by their negative zeta potential (Table 1). As the highest absolute values for zeta potential
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Published 27 Aug 2014

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

  • Nadine Haberl,
  • Stephanie Hirn,
  • Alexander Wenk,
  • Jörg Diendorf,
  • Matthias Epple,
  • Blair D. Johnston,
  • Fritz Krombach,
  • Wolfgang G. Kreyling and
  • Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

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  • resuspended in 1 mL RPMI 1640 medium (BioChrome, Berlin, Germany) supplemented with 10% fetal calf serum (Seromed, Berlin, Germany). Cell counts were measured by a CASY cell counter system (Schärfe System GmbH, Reutlingen, Germany). Cytospins were prepared by cytocentrifugation at 35g for 6 min and stained
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Published 19 Dec 2013
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