Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• receptor, gastrin releasing peptide receptor (GRPR), is widely expressed in cancers of the lung, pancreas and ovaries. Gastrin releasing peptide (GRP) is an autocrine growth factor in small cell lung cancer, which has very poor patient outcomes. High affinity antagonist peptides have been developed for in

• vivo cancer imaging. In this report we decorated pegylated liposomes with a GRPR antagonist peptide and studied its interaction with, and accumulation within, lung cancer cells. Results: An N-terminally cysteine modified GRPR antagonist (termed cystabn) was synthesised and shown to inhibit cell growth

• targeting has potential for enhancing drug accumulation in resistant cancer cells. Keywords: bombesin; GRPR; liposome; lung cancer; targeting; Introduction Small-cell lung cancer (SCLC) accounts for approximately one in five lung cancer diagnoses. In spite of global efforts to reduce tobacco smoking in

Multiwalled carbon nanotube based aromatic volatile organic compound sensor: sensitivity enhancement through 1-hexadecanethiol functionalisation

• Nadra Bohli,
• Meryem Belkilani,
• Juan Casanova-Chafer,
• Eduard Llobet and
• Adnane Abdelghani

Beilstein J. Nanotechnol. 2019, 10, 2364–2373, doi:10.3762/bjnano.10.227

• of trace concentrations of toluene in exhaled breath is associated with lung cancer and can therefore be considered as a biomarker for this pathology [2][3][4]. A gas sensor is generally composed of an active sensing film or material deposited on an electrode. The sensing performance is strongly

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

• Liang Xu,
• Dekang Xu,
• Ziying Li,
• Yu Gao and
• Haijun Chen

Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189

• and synthesized, aiming to discover new steroid-based antitumor agents. In this work, we synthesized several Di derivatives and screened FZU-0021-194-P2 (P2), which showed more potent cytotoxic activities against human non-small-cell lung cancer A549 and PC9 cells. Considering that Di has a unique

• sterol structure similarly to cholesterol, P2 phytosomes (P2Ps) were prepared to further improve the water solubility of P2. The P2Ps exhibited a particle size of 53.6 ± 0.3 nm with oval shape and a zeta potential of −4.0 ± 0.7 mV. P2Ps could inhibit the proliferation of lung cancer cells more

• efficiently than Di phytosomes after 72 h of incubation time by inducing cell cycle arrest and apoptosis. The results indicated that P2Ps could be a promising anticancer formulation for non-small-cell lung cancer. Keywords: diosgenin; non-small-cell lung cancer; phytosomes; sterol structure; Introduction

Lipid nanostructures for antioxidant delivery: a comparative preformulation study

• Elisabetta Esposito,
• Maddalena Sguizzato,
• Markus Drechsler,
• Paolo Mariani,
• Federica Carducci,
• Claudio Nastruzzi,
• Giuseppe Valacchi and
• Rita Cortesi

Beilstein J. Nanotechnol. 2019, 10, 1789–1801, doi:10.3762/bjnano.10.174

• air quality all over the world. Besides being the primary cause of many respiratory diseases (e.g., chronic obstructive pulmonary disease, asthma and lung cancer), pollution is also responsible for cutaneous pathologies, spanning from skin aging, inflammation and allergy to skin cancer [1]. Cigarette

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• -transform infrared spectroscopy (FTIR), and X-ray diffraction (XRD). The antineoplastic potential of the CaP@5-FU NPs against colorectal and lung cancer cells was reported. The CaP@5-FU NPs were found to inhibit half the population (IC50) of lung adenocarcinoma (A549) cells at 32 μg/mL and colorectal (HCT

• revealed membrane bleb in both colorectal and lung cancer cell lines. Additionally, the increased intracellular ROS level confirmed the ROS inducing potential of CaP@5-FU NPs. Up-regulated proapoptotic genes (p53, caspase-3, BAX, BAK and BAD) and down-regulated antiapoptotic genes (Bcl-2 and Bcl-xL) found

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

• Jaison Jeevanandam,
• Ahmed Barhoum,
• Yen S. Chan,
• Alain Dufresne and
• Michael K. Danquah

Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• drug efflux transporter P-glycoprotein, which ensures rapid excretion of toxic substances from MSCs. Thus, MSCs are an excellent vector for low cytotoxicity anticancer drugs [4]. Sadhukha et al. demonstrated that nanoengineered MSCs home to A549 lung cancer in vivo and remain there for at least 3 h

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• to PIPAC-MIP, HINAT provides the opportunity to perform further studies concerning the treatment of peritoneal carcinomatosis or other aerosol-accessible kinds of cancer (e.g., lung cancer) on animals even smaller than pigs due to the minimal required access space for feeding nanometre-sized aerosols

Evaluating the toxicity of TiO₂-based nanoparticles to Chinese hamster ovary cells and Escherichia coli: a complementary experimental and computational approach

• Alicja Mikolajczyk,
• Natalia Sizochenko,
• Ewa Mulkiewicz,
• Anna Malankowska,
• Michal Nischk,
• Przemyslaw Jurczak,
• Seishiro Hirano,
• Grzegorz Nowaczyk,
• Adriana Zaleska-Medynska,
• Jerzy Leszczynski,
• Agnieszka Gajewicz and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2017, 8, 2171–2180, doi:10.3762/bjnano.8.216

• a water bath for 30 min at 37 °C. Cytotoxicity was determined using the Chinese hamster ovary cell line (CHO-K1) (ATCC® CCL-61™). The sensitivity of three different cell lines: CHO-K1 and two human lung (cancer and normal) cell lines (A549, BEAS-2B) to the tested nanomaterials was studied in a

A nanocomplex of C₆₀ fullerene with cisplatin: design, characterization and toxicity

• Svitlana Prylutska,
• Svitlana Politenkova,
• Kateryna Afanasieva,
• Volodymyr Korolovych,
• Kateryna Bogutska,
• Andriy Sivolob,
• Larysa Skivka,
• Maxim Evstigneev,
• Viktor Kostjukov,
• Yuriy Prylutskyy and
• Uwe Ritter

Beilstein J. Nanotechnol. 2017, 8, 1494–1501, doi:10.3762/bjnano.8.149

• , cisplatin (cis-[Pt(II)(NH3)2Cl2], Cis), is currently one of the most effective therapeutic agents used against cancer deceases, in particular, ovarian cancer, bladder cancer, esophagus cancer, lung cancer, and cancer of head and neck [1]. As an antitumor metal-containing agent Cis exerts an alkylating

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• therapeutic nano-agents [24]. Studies on melanoma [25], prostate cancer [26], breast cancer [6] and lung cancer [27] have shown the ability of MSCs to home to cancer sites in vivo. In the tumour microenvironment, MSCs play a role in the formation of the tumour stroma and support cancer metastasis [28

Needs and challenges for assessing the environmental impacts of engineered nanomaterials (ENMs)

• Michelle Romero-Franco,
• Hilary A. Godwin,
• Muhammad Bilal and
• Yoram Cohen

Beilstein J. Nanotechnol. 2017, 8, 989–1014, doi:10.3762/bjnano.8.101

Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone

• Huijuan Zhang,
• Fuqiang Wu,
• Yazhen Li,
• Xiping Yang,
• Jiamei Huang,
• Tingting Lv,
• Yingying Zhang,
• Jianzhong Chen,
• Haijun Chen,
• Yu Gao,
• Guannan Liu and
• Lee Jia

Beilstein J. Nanotechnol. 2016, 7, 1861–1870, doi:10.3762/bjnano.7.178

• cell culture A549 human lung cancer cells, human epithelial carcinoma Hela cells, human endometrial carcinoma RL95-2 cells, and human hepatocellular liver carcinoma HepG2 cells were purchased from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). A549 and Hela was grown in

Application of biclustering of gene expression data and gene set enrichment analysis methods to identify potentially disease causing nanomaterials

• Andrew Williams and
• Sabina Halappanavar

Beilstein J. Nanotechnol. 2015, 6, 2438–2448, doi:10.3762/bjnano.6.252

• obstructive pulmonary disease (COPD) or lung cancer) in mice using the whole genome gene expression tools were obtained from GEO. For each study, raw data were downloaded from GEO and normalized as described in the methods below. Biological replicates for each of the experimental conditions were averaged. All

• lung inflammation models, emphysema, chronic obstructive pulmonary disease and experiments studying lung cancer and lung tumors. Several different microarray platforms including the Illumina expression beadchip were used in these studies. The analysis was restricted to lung disease models since

PLGA nanoparticles as a platform for vitamin D-based cancer therapy

• Maria J. Ramalho,
• Joana A. Loureiro,
• Bárbara Gomes,
• Manuela F. Frasco,
• Manuel A. N. Coelho and
• M. Carmo Pereira

Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135

• acid) (PLGA) nanoparticles were studied as drug delivery vehicles for calcitriol, the active form of vitamin D3. In vitro effects of calcitriol encapsulated in PLGA nanoparticles were evaluated with respect to free calcitriol on human pancreatic cell lines, S2-013 and hTERT-HPNE, and the lung cancer

• as a cancer chemopreventive agent due to promising epidemiological, preclinical and clinical findings [4]. The protective role of vitamin D against cancer has been mainly attributed to its anti-inflammatory activity [5]. The antineoplastic activity of calcitriol in pancreatic and lung cancer is well

• -differentiated tubular adenocarcinoma and moderately metastatic subline cloned from the human pancreatic tumor cell line SUIT-2), were provided by Prof. M. A. Hollingsworth (UNMC, Nebraska, USA) [40][41]. The human lung cancer cell line, A549 (nonsmall cell lung carcinoma), [11] was kindly provided by Dr

Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme

• Yasuhiko Onishi,
• Yuki Eshita,
• Rui-Cheng Ji,
• Masayasu Onishi,
• Takashi Kobayashi,
• Masaaki Mizuno,
• Jun Yoshida and
• Naoji Kubota

Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238

• ] analyzed expression profiles of 807 genes of non-small cell lung cancer PC-14 cells after treatment with cisplatin (CDDP) incorporated in polymeric micelles (CDDP/m) versus free cisplatin. A total of 50 genes of significant differential expression between cells treated with free CDDP and CDDP/m were

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

• Claudia Strobel,
• Martin Förster and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190

• nanoparticles were also found to be cytotoxic in other cell types, such as human bronchial epithelial cells [25][27] or human lung cancer cells [26]. The extent of the adverse effects of CeO2 nanoparticles on cells seems to be cell type-dependent. This applies also for subsets of endothelial cells which have

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

• Moritz Nazarenus,
• Qian Zhang,
• Mahmoud G. Soliman,
• Pablo del Pino,
• Beatriz Pelaz,
• Susana Carregal-Romero,
• Joanna Rejman,
• Barbara Rothen-Rutishauser,
• Martin J. D. Clift,
• Reinhard Zellner,
• G. Ulrich Nienhaus,
• James B. Delehanty,
• Igor L. Medintz and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1477–1490, doi:10.3762/bjnano.5.161

• . Scheme depicting the different mechanisms of cellular endocytosis. Reproduced with permission from [41]. Copyright (2011) Elsevier. Fluorescence microscopy image showing the granular structure of internalized NPs inside A549 lung cancer cells (two types of iron oxide NPs with different surface chemistry

• internalized by an A549 lung cancer cell into an intracellular vesicle (here the lysosome [165]) and is thus clearly localized. The microparticle is filled with a pH-sensitive fluorophore (SNARF, from Invitrogen, now LifeTech) linked to dextran and the acidic pH of the lysosome is reported by the yellow

PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

• Paula M. Castillo,
• Mario de la Mata,
• Maria F. Casula,
• José A. Sánchez-Alcázar and
• Ana P. Zaderenko

Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144

• surface modification of the nanoparticles by polyethylene glycol enables loading a large amount of camptothecin. While the unloaded nanoparticles do not induce apoptosis in the H460 lung cancer cell line, the camptothecin-loaded nanoparticle formulations exhibit remarkable pro-apoptotic activity. These

• were taken so that the provided final concentration of CPT was identical to that used in the pure CPT control. According to our results, neither USM nor USM-PEG nanoparticles induce apoptosis in lung cancer cell line cultures H460, while USM[CPT] and USM-PEG[CPT], as well as CPT itself, induced

• retain the biological activity of CPT and exhibit remarkable cytotoxic activity towards H460 lung cancer cell line cultures. Remarkably, it was found that iron oxide superparamagnetic nanoparticles synthesized by co-precipitation method can be loaded with CPT. By the proposed nanoparticle surface

Growth behaviour and mechanical properties of PLL/HA multilayer films studied by AFM

• Cagri Üzüm,
• Johannes Hellwig,
• Narayanan Madaboosi,
• Dmitry Volodkin and
• Regine von Klitzing

Beilstein J. Nanotechnol. 2012, 3, 778–788, doi:10.3762/bjnano.3.87

• . Therefore, scanning- or colloidal-probe atomic force microscopy have been widely used for studying the topography and the mechanical properties of PEMs [3][4][5][7][8]. One of the first measurements of elastic modulus with atomic force microscopy (AFM) on biological films was performed on lung-cancer cells