Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells

• Nils Bohmer and
• Andreas Jordan

Beilstein J. Nanotechnol. 2015, 6, 167–176, doi:10.3762/bjnano.6.16

• underlying uptake mechanisms would be very useful for faster and precise development of nanoparticles for clinical applications. This study aims at the identification of key proteins, which are crucial for the active uptake of iron oxide nanoparticles by HeLa cells (human cervical cancer) as a model cell

• in HeLa cells of iron oxide nanoparticles, used in this study, is mainly mediated by Caveolin-1 and CDC42. It is shown here for the first time, which proteins of the endocytotic pathway mediate the endocytosis of silica-coated iron oxide nanoparticles in HeLa cells in vitro. In future studies more

• and surface charge, were tested in HeLa cells as a model cell line. To elucidate, which molecular pathways are involved in their endocytosis, well-known endocytotic mechanisms [26][27][28] were inhibited by specific knockdown of key proteins via siRNA (Figure 1). Experimental Superparamagnetic iron

Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces

• Miao Yu,
• Nico Strohmeyer,
• Jinghe Wang,
• Daniel J. Müller and
• Jonne Helenius

Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15

• limitation, segmented polydimethylsiloxane (PDMS) masks were developed, allowing the measurement of cell adhesion to multiple substrates. To verify the utility of the masks, the adhesion of four different cell lines, HeLa (Kyoto), prostate cancer (PC), mouse kidney fibroblast and MDCK, to three extracellular

• allowed for experiments that previously were not feasible. Since the masks are economical and versatile, they can aid in the improvement of various assays. Keywords: atomic force microscopy; cell adhesion; collagen I; fibroblasts; fibronectin; HeLa; laminin; MDCK; PC3; single cell assay; single cell

• CAM has been studied by SCFS. It was shown by SCFS that collagen I binding integrins down-regulate the avidity of fibronectin binding integrins by an increased endocytosis in HeLa cells [30]. The classical SCFS setup, where the adhesion of a cantilever-bound cell to a substrate is probed, has a

Intake of silica nanoparticles by giant lipid vesicles: influence of particle size and thermodynamic membrane state

• Florian G. Strobl,
• Florian Seitz,
• Christoph Westerhausen,
• Armin Reller,
• Adriano A. Torrano,
• Christoph Bräuchle,
• Achim Wixforth and
• Matthias F. Schneider

Beilstein J. Nanotechnol. 2014, 5, 2468–2478, doi:10.3762/bjnano.5.256

• −20 mV for HeLa cells and −30 mV for red blood cells were measured [49]. The dotted curve in Figure 4 shows the expected electrostatic force between a cationic particle with ζ = +30 mV and a cell membrane with ζ = −30 mV in a medium with an ionic strength of I = 160 mM. The physical forces in this

Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects

• Li Shang,
• Karin Nienhaus,
• Xiue Jiang,
• Linxiao Yang,
• Katharina Landfester,
• Volker Mailänder,
• Thomas Simmet and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248

• surface functionalizations and investigated their interactions with various human cell lines, in particular HeLa cells and mesenchymal stem cells (MSCs). Of note, these studies were carried out in phosphate buffered saline (PBS), pH 7.4, or serum-free DMEM, so that we could probe interactions between

•  2 shows representative two-color merged fluorescence images recorded at selected times during the exposure of cultured HeLa cells to DPA-QDs in PBS and DHLA-AuNCs in DMEM solution. The cell membrane and the NPs are depicted in red and green color, respectively; colocalization is shown in yellow

• apparent that the uptake efficiency of small NPs in HeLa cells is affected by both dynasore and chlorpromazine [31][34]. Chlorpromazine reduced both the membrane-associated and the intracellular fractions. Because chlorpromazine disturbs clathrin-coated pit formation, it lowers both the binding capacity of

Interaction of dermatologically relevant nanoparticles with skin cells and skin

• Annika Vogt,
• Fiorenza Rancan,
• Sebastian Ahlberg,
• Berouz Nazemi,
• Chun Sik Choe,
• Maxim E. Darvin,
• Sabrina Hadam,
• Ulrike Blume-Peytavi,
• Kateryna Loza,
• Jörg Diendorf,
• Matthias Epple,
• Christina Graf,
• Eckart Rühl,
• Martina C. Meinke and
• Jürgen Lademann

Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245

• , particle size and the size of aggregates formed in physiological environments can become limiting factors. Similar results were obtained for similarly sized silica particles (55 ± 2 nm) with and without APS-functionalization in HeLa cells [35]. Also in this case, the APS-functionalized particles were

Inorganic Janus particles for biomedical applications

• Isabel Schick,
• Steffen Lorenz,
• Dominik Gehrig,
• Stefan Tenzer,
• Wiebke Storck,
• Karl Fischer,
• Dennis Strand,
• Frédéric Laquai and
• Wolfgang Tremel

Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244

• Chemistry. CLSM images of HeLa cells co-incubated with Au@MnO@SiO2-Atto495 Janus particles (green) for 24 h at 37 °C (c(Mn2+) = 100 µg/mL). a) λex = 488 nm, cell nuclei were stained using DAPI, b) two-photon image of the same sample, λex(2P) = 970 nm. Scale: 10 µm. Adapted with permission from [39

Carbon nano-onions (multi-layer fullerenes): chemistry and applications

• Juergen Bartelmess and
• Silvia Giordani

Beilstein J. Nanotechnol. 2014, 5, 1980–1998, doi:10.3762/bjnano.5.207

• evidenced by a reduced secretion of the inflammatory cytokine IL-1β, and a pronounced decrease in the recruitment of neutrophils and monocytes following injection into mice. Subsequently, in two recent studies, we investigated the effects of two different, fluorophore functionalized CNOs on HeLa Kyoto [40

• promising material for biomedical applications. Recently we demonstrated the cellular imaging of HeLa Kyoto [40] and MCF-7 cells [41] after incubating them with azaBODIPY- or BODIPY-functionalized CNOs (Scheme 8 and Figure 7). In both cases the CNO conjugates were readily internalized by the cells. In the

• (green) and nuclei stain Hoechst (blue) (right). Reprinted with permission from [39]. Copyright 2013 John Wiley and Sons. Confocal images of azaBODIPY-CNOs in HeLa Kyoto cells (left) and BODIPY-CNOs in MCF-7 cells (right). The blue luminescence is due to Hoechst 33342 nuclear stain. Reproduced with

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• or macropinocytosis is involved in both the uptake of larger aggregates of 40 nm NPs and 1 µm particles. Our findings are in agreement with other studies that showed a reduced uptake of 40 nm carboxylated polystyrene particles in HeLa and 1321N1 cells in the presence of cytochalasin A [52] and by

Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy

• Adriano A. Torrano and
• Christoph Bräuchle

Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173

• derived from the cervix carcinoma (HeLa). The absolute number of intracellular silica nanoparticles within the first 24 h was determined and shown to be cell type-dependent. As a second case study, Particle_in_Cell-3D was used to assess the uptake kinetics of 8 nm and 30 nm ceria nanoparticles interacting

• . In this section, we want to present in detail how Particle_in_Cell-3D was used to study the cell type-dependent uptake of 310 nm silica nanoparticles into human vascular endothelial cells (HUVEC) and cancer cells derived from the cervix carcinoma (HeLa). The nanoparticle uptake by single cells was

• for HeLa cells. However, after 10 or 24 h of interaction, the amount of particles taken up by HeLa cells strikingly exceeded the amount of silica particles taken up by HUVEC cells. Characterization of silica nanoparticles In order to allow for the investigation with live-cell imaging, silica

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• prepared AuNPs as an effective anticancer agent, MTT assays were performed against three different cancer cells lines (human colorectal cancer cells (HCT116), human cervical cancer cells (HeLa) and squamous carcinoma cells (SCC-7)) by treating them with AuNPs. The results are shown in Figure 4B, 4C and 4D

• µg/mL and 32.68 µg/mL for Hela, which shows that these nanoparticles cause 50% inhibition of the cancer cell at a lower concentration in comparison to the fibroblasts. This can be attributed due to the difference in the surface charge at different pH conditions. The surface charge on BSA-AuNPs at

• OVA > BGG > LYS > BSA > BHG > HIS, OVA > BGG > LYS > BHG > BSA > HIS and OVA > BGG > BSA > BHG > LYS > HIS for the HCT116, HeLa and SCC-7 cell lines, respectively (Table S3, Supporting Information File 1). The MTT assay on the blank proteins showed cell viabilities greater than 80% (Figure S7

Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer

• Amanee D Salaam,
• Patrick Hwang,
• Roberus McIntosh,
• Hadiyah N Green,
• Ho-Wook Jun and
• Derrick Dean

Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107

• ]. Liang et al. demonstrated that DOX-loaded micelles can efficiently use the tumor-targeting function of RGD sequence to deliver the drug into HeLa cells [38]. Tian et al. showed that iRGD exosomes delivered DOX specifically to tumor tissues and inhibited tumor growth without overt toxicity [39]. Zhou et

Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

• Marcus Bleicher,
• Lucas Burigo,
• Marco Durante,
• Maren Herrlitz,
• Michael Krämer,
• Igor Mishustin,
• Iris Müller,
• Francesco Natale,
• Igor Pshenichnov,
• Stefan Schramm,
• Gisela Taucher-Scholz and
• Cathrin Wälzlein

Beilstein J. Nanotechnol. 2012, 3, 556–563, doi:10.3762/bjnano.3.64

• , for long-term live-cell observations. Image acquisition is done by a Hamamatsu C7190 EB-CCD camera. Photobleaching of GFP-tagged H2B in living HeLa cells by the 405 laser is demonstrated in Figure 9. By turning and panning of the laser circle, the logo of the Beilstein-Institut was visualized by

• deflection of the primary ions leads to a natural "diffusion" of the radial dose. Living HeLa cells expressing histone H2B tagged to GFP were photobleached. Bleaching within a region of three sectors of a circle depletes fluorescence from the bleached region. Colors of the three regions were adjusted with