Search results
Search for "HeLa cells" in Full Text gives 59 result(s) in Beilstein Journal of Nanotechnology.
Intake of silica nanoparticles by giant lipid vesicles: influence of particle size and thermodynamic membrane state
Beilstein J. Nanotechnol. 2014, 5, 2468–2478, doi:10.3762/bjnano.5.256
- −20 mV for HeLa cells and −30 mV for red blood cells were measured [49]. The dotted curve in Figure 4 shows the expected electrostatic force between a cationic particle with ζ = +30 mV and a cell membrane with ζ = −30 mV in a medium with an ionic strength of I = 160 mM. The physical forces in this
Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects
Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248
- surface functionalizations and investigated their interactions with various human cell lines, in particular HeLa cells and mesenchymal stem cells (MSCs). Of note, these studies were carried out in phosphate buffered saline (PBS), pH 7.4, or serum-free DMEM, so that we could probe interactions between
- 2 shows representative two-color merged fluorescence images recorded at selected times during the exposure of cultured HeLa cells to DPA-QDs in PBS and DHLA-AuNCs in DMEM solution. The cell membrane and the NPs are depicted in red and green color, respectively; colocalization is shown in yellow
- apparent that the uptake efficiency of small NPs in HeLa cells is affected by both dynasore and chlorpromazine [31][34]. Chlorpromazine reduced both the membrane-associated and the intracellular fractions. Because chlorpromazine disturbs clathrin-coated pit formation, it lowers both the binding capacity of
Interaction of dermatologically relevant nanoparticles with skin cells and skin
Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245
- , particle size and the size of aggregates formed in physiological environments can become limiting factors. Similar results were obtained for similarly sized silica particles (55 ± 2 nm) with and without APS-functionalization in HeLa cells [35]. Also in this case, the APS-functionalized particles were
Inorganic Janus particles for biomedical applications
Beilstein J. Nanotechnol. 2014, 5, 2346–2362, doi:10.3762/bjnano.5.244
- Chemistry. CLSM images of HeLa cells co-incubated with Au@MnO@SiO2-Atto495 Janus particles (green) for 24 h at 37 °C (c(Mn2+) = 100 µg/mL). a) λex = 488 nm, cell nuclei were stained using DAPI, b) two-photon image of the same sample, λex(2P) = 970 nm. Scale: 10 µm. Adapted with permission from [39
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy
Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173
- for HeLa cells. However, after 10 or 24 h of interaction, the amount of particles taken up by HeLa cells strikingly exceeded the amount of silica particles taken up by HUVEC cells. Characterization of silica nanoparticles In order to allow for the investigation with live-cell imaging, silica
- intensity showed a Gaussian distribution with a mean value of 48090 pixel intensities per nanoparticle for silica particles in the cell medium for HeLa cells and 49430 pixel intensities per nanoparticle for silica particles in the cell medium for HUVEC cells. Quantification of silica-nanoparticle uptake In
- stacks were then acquired and analyzed with Particle_in_Cell-3D. Figure 2 shows representative 3D perspectives of silica nanoparticles internalized by HUVEC and HeLa cells after 3 and 24 h. By using this method it was possible to precisely localize and quantify the particles interacting with the cells
Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity
Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158
- Media (RPMI) 1640 (SIGMA) containing 10% FBS and 1% antibiotic-antimycotic. The NIH-3T3 and HeLa cells were originally obtained from the Korean Cell line Bank (Seoul, Korea). The SCC7 and HCT-116 cells were purchased from the American Type Culture Collection. All of the cells were cultured in a
Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer
Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107
- ]. Liang et al. demonstrated that DOX-loaded micelles can efficiently use the tumor-targeting function of RGD sequence to deliver the drug into HeLa cells [38]. Tian et al. showed that iRGD exosomes delivered DOX specifically to tumor tissues and inhibited tumor growth without overt toxicity [39]. Zhou et
Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies
Beilstein J. Nanotechnol. 2012, 3, 556–563, doi:10.3762/bjnano.3.64
- , for long-term live-cell observations. Image acquisition is done by a Hamamatsu C7190 EB-CCD camera. Photobleaching of GFP-tagged H2B in living HeLa cells by the 405 laser is demonstrated in Figure 9. By turning and panning of the laser circle, the logo of the Beilstein-Institut was visualized by
- deflection of the primary ions leads to a natural "diffusion" of the radial dose. Living HeLa cells expressing histone H2B tagged to GFP were photobleached. Bleaching within a region of three sectors of a circle depletes fluorescence from the bleached region. Colors of the three regions were adjusted with
Other Beilstein-Institut Open Science Activities
