Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• ]. After spotting, the arrays were blocked with 10% bovine serum albumin (BSA) to reduce unspecific binding of the protein to the polymer. Next, samples were washed and incubated for 1 h with 0.5 µg solution of STV-FITC in phosphate buffered saline (PBS). The observed STV-FITC fluorescence is homogenous

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• denoted as HTRA1-488 and HTRA2-488 in the following. Fluorescein-isothiocyanate (FITC)-labelled bovine serum albumin (denoted as BSA-FITC in the following) was obtained from Sigma-Aldrich (Germany). Synthesis of the protein-loaded calcium phosphate nanoparticles The nanoparticles were synthesized as

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• serum albumin (BSA) were added and shaken for 10 min. Thereafter the sample was centrifuged (7,500g 30 min) to remove unbound protein and AuNPs were re-suspended in 1 mL of 2 mM borate buffer pH 8.7 containing 5% sucrose, 2% glycerol, 0.5% BSA, and 0.01% Tween. The washing step was repeated once and the

• described above to adjust the pH. The appropriated amount of goat anti-rabbit IgG and horseradish peroxidase were added and allowed to react under agitation in a carrousel for 30 min. Afterwards, sucrose was incorporated to a final concentration of 5% and incubated for 30 min. Finally, 160 μL of 3% bovine

Performance of colloidal CdS sensitized solar cells with ZnO nanorods/nanoparticles

• Anurag Roy,
• Partha Pratim Das,
• Mukta Tathavadekar,
• Sumita Das and
• Parukuttyamma Sujatha Devi

Beilstein J. Nanotechnol. 2017, 8, 210–221, doi:10.3762/bjnano.8.23

• Division, CSIR-National Chemical Laboratory, Pune 411 008, India 10.3762/bjnano.8.23 Abstract As an alternative photosensitizer in dye-sensitized solar cells, bovine serum albumin (BSA) (a nonhazardous protein) was used in the synthesis of colloidal CdS nanoparticles (NPs). This system has been employed

• dots (QDs) we followed a bioinspired strategy to synthesize such systems. In this work, bovine serum albumin (BSA) is selected as a biotemplating agent. BSA is a ubiquitous protein mainly present in blood and is a commonly used reagent in biological studies. Here, we have synthesized functionalized

• and ethanol were purchased from Merck Limited, Germany. Bovine serum albumin was purchased from Sigma-Aldrich, USA. Zn(NO3)3 and Zn(CH3COO)2 were purchased from Merck Limited, Germany. All the chemicals were used without any further purification. Synthesis of CdS nanoparticles The CdS NPs were

Effective intercalation of zein into Na-montmorillonite: role of the protein components and use of the developed biointerfaces

• Ana C. S. Alcântara,
• Margarita Darder,
• Pilar Aranda and
• Eduardo Ruiz-Hitzky

Beilstein J. Nanotechnol. 2016, 7, 1772–1782, doi:10.3762/bjnano.7.170

• intercalation of gelatin into montmorillonite [4], other biohybrids also based on the assembly of smectite clays and proteins (e.g., bovine serum albumin, gelatin, casein or soy) have been vastly studied [5][6][7][8][9][10]. However, protein adsorption on montmorillonite clay can be considered a complex process

• proteins intercalated in sodium montmorillonite, such as bovine serum albumin (BSA) [5]. Therefore, the new route 2 seems more effective to achieve the incorporation of zein molecules into the intracrystalline space of sodium montmorillonite. The infrared spectra of pristine MMT, zein, and Z-MMT_S2

High performance Ce-doped ZnO nanorods for sunlight-driven photocatalysis

• Bilel Chouchene,
• Tahar Ben Chaabane,
• Lavinia Balan,
• Emilien Girot,
• Kevin Mozet,
• Ghouti Medjahdi and
• Raphaël Schneider

Beilstein J. Nanotechnol. 2016, 7, 1338–1349, doi:10.3762/bjnano.7.125

• (Figure 12a) [58]. Glucose, a reducing sugar (100 mg/L), urea (100 mg/L) or Na2S (10 mg/L) only slightly decreased the photodegradation rate of Orange II at pH 7 (Figure 12b). Bovine serum albumin (BSA) (10 mg/L) and aniline (10 mg/L) had a more pronounced inhibition effect (degradation of Orange II in

Surface coating affects behavior of metallic nanoparticles in a biological environment

• Darija Domazet Jurašin,
• Marija Ćurlin,
• Ivona Capjak,
• Tea Crnković,
• Marija Lovrić,
• Michal Babič,
• Daniel Horák,
• Ivana Vinković Vrček and
• Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 246–262, doi:10.3762/bjnano.7.23

• broad range of mammalian cells, was used as a model biological fluid. Bovine serum albumin (BSA) served as a model serum protein due to its biological relevance and high significance in biomedical applications. Albumin is the most abundant protein in mammalian blood plasma and has outstanding buffering

• phosphate as buffering agent (product number 12-614Q) was obtained from Lonza (Verviers, Belgium). Bovine serum albumin (product number A-7906, Sigma-Aldrich Chemie GmbH, Steinheim, Germany) was used as received without further purification. The plastic and glassware used for chemical analysis were from

• bromide (CTA), poly(vinylpyrrolidone) (PVP), poly(L-lysine) (PLL), bovine serum albumin (BSA), Brij 35 (Brij), Tween 20 (Tween) and D-mannose (MAN). Silver nanoparticles coated with sodium bis(2-ethylhexyl) sulfosuccinate (AOTAgNP), cetyltrimethylammonium bromide (CTAAgNP), poly(vinylpyrrolidone) (PVPAgNP

Optimized design of a nanostructured SPCE-based multipurpose biosensing platform formed by ferrocene-tethered electrochemically-deposited cauliflower-shaped gold nanoparticles

• Wicem Argoubi,
• Maroua Saadaoui,
• Sami Ben Aoun and
• Noureddine Raouafi

Beilstein J. Nanotechnol. 2015, 6, 1840–1852, doi:10.3762/bjnano.6.187

• , produced in goat), bovine serum albumin (98%), ascorbic acid, dopamine, H2O2, silica gel 60 mesh and F254 nm fluorescent silica-coated aluminum TLC plates were purchased from Sigma-Aldrich (Germany) and used as received without further purification. All solvents used were of analytical grade and were used

Probing fibronectin–antibody interactions using AFM force spectroscopy and lateral force microscopy

• Andrzej J. Kulik,
• Małgorzata Lekka,
• Kyumin Lee,
• Grazyna Pyka-Fościak and
• Wieslaw Nowak

Beilstein J. Nanotechnol. 2015, 6, 1164–1175, doi:10.3762/bjnano.6.118

• for a similar type of interaction between an antigen and an antibody (i.e., bovine serum albumin and its monoclonal antibody) [35]. Our experiments do not allow for determination of which region (module) of FN interacts with Mab. As the bond dissociation is a nonequilibrium, dynamic process

Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development

• Ulrike Taylor,
• Daniela Tiedemann,
• Christoph Rehbock,
• Wilfried A. Kues,
• Stephan Barcikowski and
• Detlef Rath

Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66

• ) and oligonucleotide conjugated AuNP (diameter 7.3 nm, 94 biomolecules per particle, Zeta potential −32 mV) which were tested by using bovine sperm, as well as bovine serum albumin (BSA) coated gold (diameter 6–20 nm), silver (diameter 11 nm; AgNP) and various gold silver alloy nanoparticles (silver

• concentration was 10 µg/mL and all particles were conjugated with bovine serum albumin. Reproduced with permission from [50]. Copyright 2014 Royal Society of Chemistry. Representative laser scanning microscope images of porcine cumulus–oocyte complexes after 46 h co-incubation during in vitro maturation. (A

• ) conjugated to bovine serum albumin (BSA) as measured by disc centrifugation. xc has been calculated by log-normal fitting. (B) Oocyte maturation rates after exposure to AgNP in situ or ex situ bioconjugated to BSA. *p < 0.05. Blastocyst development rates after microinjection of nanoparticles into 2-cell

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• . The silica-coated magnetic and fluorescent NPs were then conjugated with bovine serum albumin (BSA) by using N-hydroxysuccinimide (NHS) as cross linker. The synthesized NPs were then characterized by XRD, IR, TEM, absorption and fluorescence spectroscopy. The XRD diffraction peaks indicate the cubic

The fate of a designed protein corona on nanoparticles in vitro and in vivo

• Denise Bargheer,
• Julius Nielsen,
• Gabriella Gébel,
• Markus Heine,
• Sunhild C. Salmen,
• Roland Stauber,
• Horst Weller,
• Joerg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5

• nm) is much smaller than the adsorbent. If the nanoparticle and protein are in the same size, the Langmuir adsorption formalism seems not to be valid. This was discussed by Liu et al. using small CeO2-particles and bovine serum albumin with similar size of 7 nm [36]. The smaller surface free energy

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• the presence of the proteins bovine serum albumin (BSA) and casein the fluorescence intensities were discernibly decreased, no matter of which size the NPs were. We speculate that a protein coating produced by passive adsorption of the proteins to the NPs is responsible for this observed reduction in

Biopolymer colloids for controlling and templating inorganic synthesis

• Laura C. Preiss,
• Katharina Landfester and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2014, 5, 2129–2138, doi:10.3762/bjnano.5.222

• synthetic polymers (see Section 4 in [60] for a review), but only a limited number of works are found for biopolymers. Li et al. [61] prepared cross-linked chitosan microspheres and immobilized bovine serum albumin covalently on their surface. On the resulting particles, silica was formed by a sol–gel

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

Imaging the intracellular degradation of biodegradable polymer nanoparticles

• Anne-Kathrin Barthel,
• Martin Dass,
• Melanie Dröge,
• Jens-Michael Cramer,
• Daniela Baumann,
• Markus Urban,
• Katharina Landfester,
• Volker Mailänder and
• Ingo Lieberwirth

Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201

• bovine serum albumin (BSA), which is a main component of FCS that is added to the cell medium. The results showed that BSA significantly increased the exocytosis of the tested PLGA nanoparticles [28]. Given that we used FCS in the cell medium, the high exocytosis might be responsible for the significant

In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics

• Wolfgang G. Kreyling,
• Stefanie Fertsch-Gapp,
• Martin Schäffler,
• Blair D. Johnston,
• Nadine Haberl,
• Christian Pfeiffer,
• Jörg Diendorf,
• Carsten Schleh,
• Stephanie Hirn,
• Manuela Semmler-Behnke,
• Matthias Epple and
• Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180

• proteins. This plethora of corona proteins will mediate the NP navigation within body fluids and across membranes and provides a plausible interpretation of the very many sites of retention and accumulation of NP in various organs and tissues of the organism. Bovine serum albumin (BSA) depletion in the

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• ]. Biomolecules have been reported to interact with gold salts and reduce them into metallic gold, acting both as a reductant and stabilizer [12][17][18][19][20][21]. Proteins, such as bovine serum albumin, silk fibroin protein, chicken egg white lysozyme, α-amylase, green fluorescent protein and apoferritin have

• mg/mL and 0.5 mM, respectively. These conditions did not result in the formation of AuNPs. The addition of Ag ions to a mixture containing Au ions has been reported to form AuNPs in the presence of bovine serum albumin (BSA) [33]. Hence, we added AgNO3 at a ratio of 5:1 between the Au and Ag ions and

• histone (HIS), chicken egg white lysozyme (LYS), ovalbumin (OVA), bovine serum albumin (BSA), bovine hemoglobin (BHG), bovine gamma globulin (BGG), glucose oxidase (Aspergillus niger) (GOX), and trypsin (porcine pancreas, TRY), used for the experiments were obtained from Sigma (USA) as a lyophilized

Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique

• Alina Maria Holban,
• Valentina Grumezescu,
• Alexandru Mihai Grumezescu,
• Bogdan Ştefan Vasile,
• Roxana Truşcă,
• Rodica Cristescu,
• Gabriel Socol and
• Florin Iordache

Beilstein J. Nanotechnol. 2014, 5, 872–880, doi:10.3762/bjnano.5.99

• polymeric microspheres. Thus, Socol et al., [43], firstly reported the novel deposition of PLGA–PVA, PLGA–PVA–BSA (bovine serum albumin) and PLGA–PVA–CS microspheres by matrix assisted pulsed laser evaporation (MAPLE) technique. SEM images of thin coatings reveal homogeneous and spherical-shaped particles

• in the micrometric range. The average diameter of PLGA–PVA, PLGA–PVA–BSA (bovine serum albumin) and PLGA–PVA–CS particles ranged from 180 to 250 nm. Grumezescu et al., [34], reported the MAPLE fabrication of PLA–PVA–UA microsphere thin coatings. These thin coatings possessed a homogeneous shape and

Porous polymer coatings as substrates for the formation of high-fidelity micropatterns by quill-like pens

• Michael Hirtz,
• Marcus Lyon,
• Wenqian Feng,
• Andrea E. Holmes,
• Harald Fuchs and
• Pavel A. Levkin

Beilstein J. Nanotechnol. 2013, 4, 377–384, doi:10.3762/bjnano.4.44

• micrometres. The bromophenol blue arrays on HEMA support were used to detect the presence of bovine serum albumin (BSA). In the presence of BSA, the fluorescence spectrum observed from the bromophenol blue microarray exhibited a significant red shift of the maximum emission wavelength. Our results show that

• samples [12][13]. After preparing 10 × 10 spot microarrays using bromophenol blue solution as shown in the inset of Figure 6b, the patterns were either incubated with a 0.5 µL droplet of an analyte solution containing bovine serum albumin (BSA) or a clear solution to give a negative control. Fluorescence

Macromolecular shape and interactions in layer-by-layer assemblies within cylindrical nanopores

• Thomas D. Lazzara,
• K. H. Aaron Lau,
• Wolfgang Knoll,
• Andreas Janshoff and
• Claudia Steinem

Beilstein J. Nanotechnol. 2012, 3, 475–484, doi:10.3762/bjnano.3.54

• molecular recognition of biotinylated-bovine serum albumin (b-BSA) by avidin. Avidin has four biotin-binding sites, whereas the b-BSA used has 13 biotin molecules per protein on average. Avidin with a mass of MW = 66–69 kDa, and which is positively charged at neutral pH, was first adsorbed onto the

• Calbiochem (purity 12.9 units/mg). Biotinylated-bovine serum albumin (b-BSA) with 13 mol biotin/mol albumin, poly(sodium 4-styrenesulfonate) (PSS) (Mw = 70 kDa), poly(allylamine hydrochloride) PAH (Mw = 50–65 kDa), CuCl2, NaCl, and 16-mercaptohexadecanoic acid (90%) were purchased from Sigma Aldrich (St

• serum albumin was compared with that of linear polyelectrolytes (linear-PEs), both species being of similar molecular weight. LbL deposition within the cylindrical AAO geometry for different pore diameters (d0 = 25–80 nm) for the various macromolecular species, showed that the multilayer film growth was

Analysis of fluid flow around a beating artificial cilium

• Mojca Vilfan,
• Gašper Kokot,
• Andrej Vilfan,
• Natan Osterman,
• Blaž Kavčič,
• Igor Poberaj and
• Dušan Babič

Beilstein J. Nanotechnol. 2012, 3, 163–171, doi:10.3762/bjnano.3.16

• diameter 55 nm [24]) in water. To prevent aggregation of the beads, we coated them with BSA (bovine serum albumin), 10 mg/mL, for 4 h in an ultrasonic bath. One end of the assembled chain was attached to the surface through prefabricated ferromagnetic-nickel anchoring sites. The nickel dots were

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• bovine serum albumin (BSA) protein as a control did not elicit any change in T2 (Figure 5b). More recently, MRSw biosensors, capable of detecting soluble tumor biomarker proteins (such as CA-125, VEGF, and α-fetoprotein) were described, and used for parallel detection of multiple markers in blood samples