Using gold nanoparticles to detect single-nucleotide polymorphisms: toward liquid biopsy

• María Sanromán Iglesias and
• Marek Grzelczak

Beilstein J. Nanotechnol. 2020, 11, 263–284, doi:10.3762/bjnano.11.20

• reported the detection of mutations in exon 19 and exon 21 of the epidermal growth factor receptor (EGFR) isolated from both the lung cancer cell lines and the cancer tissues of patients with non-small-cell lung cancer [66]. The citrate-stabilized gold nanoparticles underwent selective aggregation upon the

Phase inversion-based nanoemulsions of medium chain triglyceride as potential drug delivery system for parenteral applications

• Eike Folker Busmann,
• Dailén García Martínez,
• Henrike Lucas and
• Karsten Mäder

Beilstein J. Nanotechnol. 2020, 11, 213–224, doi:10.3762/bjnano.11.16

• the aqueous Kolliphor HS 15 solution to normal human dermal fibroblasts (NHDF) and mouse embryonic fibroblasts (3T3) was investigated. The impact of the nanoemulsions and the surfactant solution on the cell viability of the two cell lines after 4 and 24 h is illustrated in Figure 7. The viability of

• certain concentration cKol. The 3T3 cells responded more sensitively to the nanoemulsions and the pure surfactant solution. Furthermore, the long incubation time of 24 h led to cell toxicity at slightly lower concentrations for both cell lines. For comparison, for the current nanoemulsions, the cell

• Supporting Information File 1. The IC50 values of Kolliphor HS 15 in the different nanoemulsions and the pure surfactant solution were similar for the different cell lines and incubation times employed. Thus, mainly the surfactant Kolliphor HS 15 inhibited cell viability at high concentration, since all

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

• pheophorbide a when incorporated in micellar, vesicular or worm-like PEO-PCL vectors in two cell lines (FaDu or HCT 116) [66][67]. No strong improvement was observed for elongated systems working on 3D spheroids and, interestingly, a micelle/vesicle mixture led to synergistic effects on HCT 116 cells but

Internalization mechanisms of cell-penetrating peptides

• Ivana Ruseska and
• Andreas Zimmer

Beilstein J. Nanotechnol. 2020, 11, 101–123, doi:10.3762/bjnano.11.10

• -siRNA/miRNA delivery [17]. It has been reported that CPP-siRNA conjugates have reduced transient and stable expression of reporter transgenes in a number of mammalian cell lines [18]. In this case, thiol-containing siRNAs were synthesized and conjugated to penetratin or transportan by a reducible

• complexes with cargo molecules through electrostatic interaction. Pep-1 has successfully been used to deliver small peptides and proteins into cells, while MPG has shown to efficiently deliver small interfering RNA (siRNA) into cultured cell lines [3][10]. The interplay between hydrophilic and hydrophobic

• in a broad spectrum of cell lines. It has a length of 21 amino acids and consists of three domains, each conferring a specific function: (i) a hydrophobic, tryptophan-rich sequence required for the interaction with macromolecules and cell-membrane targeting, (ii) a hydrophilic sequence, rich in

The different ways to chitosan/hyaluronic acid nanoparticles: templated vs direct complexation. Influence of particle preparation on morphology, cell uptake and silencing efficiency

• Arianna Gennari,
• Julio M. Rios de la Rosa,
• Erwin Hohn,
• Maria Pelliccia,
• Enrique Lallana,
• Roberto Donno,
• Annalisa Tirella and
• Nicola Tirelli

Beilstein J. Nanotechnol. 2019, 10, 2594–2608, doi:10.3762/bjnano.10.250

• images were analysed using ImageJ software (v1.49p, http://rsb.info.nih.gov/ij). Cell studies HCT-116 and RAW 264.7 cell lines were cultured in complete media (McCoy’s 5A or high glucose DMEM, respectively) under standard conditions for cell culture (5% v/v CO2 in air, 37 °C). Further details of the

• -based cationic nanocarriers [40][41]. Next, we analysed the nanoparticle uptake in the two cell lines for up to 24 h; we tracked the fluorescence associated to nanoparticles in cell lysates, which accounts for both membrane-bound and internalized materials [10]. We used fluorescently labelled chitosan

• apparent (Figure 5B) that the uptake kinetics was not influenced by the preparative method. With both cell lines and both chitosan MW, the particle uptake kinetics showed evidence of saturation and was not affected by the preparative method. We have previously reported that RAW 264.7 macrophages take up

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• with an unidentified endogenous ligand. The expression of bombesin-related peptides and the BB receptors by SCLC cell lines and primary tumours have been widely studied for the past three decades [3][4][5][6]. GRP and NMB secretion by SCLC is known to cause an autocrine growth loop that drives tumour

pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

• Saban Kalay,
• Yurij Stetsyshyn,
• Volodymyr Donchak,
• Khrystyna Harhay,
• Ostap Lishchynskyi,
• Halyna Ohar,
• Yuriy Panchenko,
• Stanislav Voronov and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233

• contrast to acidic pH conditions where the fluorescent intensity is absent or low. No increase in the absorption was observed when the suspension pH was increased from 7 to 10. The functionalized BNNTs are easily taken up by human normal prostate epithelium (PNT1A) and human prostate cancer cell lines

• obtained with a Carl Zeiss Evo-40 instrument under high vacuum and accelerating voltage of 10 kV. Cell culture experiments Normal prostate epithelium (PNT1A) and human prostate cancer (DU145) cell lines were grown in Dulbecco’s Modified Eagle’s Medium, supplemented with 10% fetal bovine serum and 1

• uptake of P(AA-co-FA)-functionalized BNNTs into human normal prostate epithelium (PNT1A) and human prostate cancer (DU145) cell lines, we used fluorescence microscopy with excitation at 490 nm and emission at 520 nm (Figure 9). The autofluorescence of healthy cells and cancer cells was strictly avoided

Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

• Sabrina Barbosa de Souza Ferreira,
• Gustavo Braga,
• Évelin Lemos Oliveira,
• Jéssica Bassi da Silva,
• Hélen Cássia Rosseto,
• Lidiane Vizioli de Castro Hoshino,
• Mauro Luciano Baesso,
• Wilker Caetano,
• Craig Murdoch,
• Helen Elizabeth Colley and
• Marcos Luciano Bruschi

Beilstein J. Nanotechnol. 2019, 10, 2304–2328, doi:10.3762/bjnano.10.222

• cells (FaDu and Cal27) and normal oral immortalized keratinocytes (FNB6). The three cell lines were exposed to a wide range of drug concentrations (0, 2.5, 5, 10, 20, 40, 80, 120 and 240 µM) for 24 h. Moreover, another range of drug concentrations (2000, 1500, 1000, 500, 300, 100, 20, 10 µM) was

• in tumor and healthy cell lines. The formulations prepared from solid dispersions stored at 25 °C containing P407, C974P and CUR displayed viscoelastic properties, plastic behavior with rheopexy at body temperature (which provides better retention), and increased mucoadhesive force due to the

• presence of CUR. CUR tends to be located in the core of the micelles; consequently, the drug displayed slow and complete release after 8 h but did not permeate the porcine oral mucosa. The cytotoxicity studies revealed the increase in the cytotoxic effects for some tumor cell lines (Cal 27) when

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• anti-PSA antibodies were obtained from Abcam, Cambridge, UK. Graphite-based ink was obtained from Henkel Electrodag, USA (reference code 423SS), silver chloride ink was purchased from Gwent electronic materials Ltd., UK (Product code C2130905D3), the cell lines PNT-2 and LNCap were acquired from the

• cells (lineage of LNCap and PNT-2 cells, respectively). The cell lines PNT-2 and LNCap were acquired from the Banco de Células do Rio de Janeiro (BCRJ, Rio de Janeiro, Brazil). The samples were diluted to a 1:30000 ratio. The resulting conjugate, Ab2-MNP-HRP-protein, was magnetically separated and

Incorporation of doxorubicin in different polymer nanoparticles and their anticancer activity

• Sebastian Pieper,
• Hannah Onafuye,
• Dennis Mulac,
• Jindrich Cinatl Jr.,
• Mark N. Wass,
• Martin Michaelis and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 2062–2072, doi:10.3762/bjnano.10.201

• solvent displacement and emulsion diffusion approaches and assessed their anticancer efficiency in neuroblastoma cells, including ABCB1-expressing cell lines, in comparison to doxorubicin solution. Results: The resulting nanoparticles covered a size range between 73 and 246 nm. PLGA-PEG nanoparticle

• preparations were tested for anticancer efficacy in cancer cell lines, including cell lines that express ABCB1. Results and Discussion Influence of the preparation technique on particle diameter and polydispersity index Nanoparticles based on poly(lactic-co-glycolic acid) (PLGA), a copolymer composed of

• emulsion diffusion and solvent displacement were tested for their effects on the viability of the neuroblastoma cell line UKF-NB-3, its doxorubicin-adapted sub-line UKF-NB-3rDOX20, and its vincristine-resistant sub-line UKF-NB-3rVCR1. In all three cell lines, PLA nanoparticles, PLGA nanoparticles prepared

Gold-coated plant virus as computed tomography imaging contrast agent

• Alaa A. A. Aljabali,
• Mazhar S. Al Zoubi,
• Khalid M. Al-Batanyeh,
• Ali Al-Radaideh,
• Mohammad A. Obeid,
• Abeer Al Sharabi,
• Walhan Alshaer,
• Bayan AbuFares,
• Tasnim Al-Zanati,
• Murtaza M. Tambuwala,
• Naveed Akbar and
• David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

• −53.8 ± 2.4 mV upon antibody coating of the particles. The zeta potential value of VCAM1-PEG5000Au-CPMV becomes more negative [38], which is consistent with the literature [42]. In vitro fluorescent cell imaging Confocal fluorescence microscopy was performed on the cell lines to demonstrate the

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

• Liang Xu,
• Dekang Xu,
• Ziying Li,
• Yu Gao and
• Haijun Chen

Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189

• of that of Di [12]. Diosgenin–imidazolium salt derivatives were also synthesized and displayed significant cytotoxic activities against several human cancer cell lines [13]. One of novel 22-oxo-26-selenocyanocholestanic steroids based on Di synthesized by Fernández-Herrera et al. exhibited remarkable

• steroid structure of Di, there are abundant electrons at double-bond sites for electrophilic addition. Based on this feature, we designed and synthesized a series of Di derivatives. We investigated the cytotoxicity of these Di derivatives in different cancer cell lines and their IC50 values were

• -ray crystallographic analysis. CCDC1844665 contains the crystallographic data of P2. The data can be obtained free of charge from the Cambridge Crystallographic Data Center. Antiproliferative activity of P2 The anticancer activity of Di and its analogue P2 was tested in human lung cancer cell lines

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

• Barbara Pem,
• Igor M. Pongrac,
• Lea Ulm,
• Ivan Pavičić,
• Valerije Vrček,
• Darija Domazet Jurašin,
• Marija Ljubojević,
• Adela Krivohlavek and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

• continuous cell lines that can be properly maintained for a long time without being transformed. Daphnia magna was used as the representative aquatic organism model to evaluate ecotoxicity. The comparison of the observed results with data published on other AgNP and AuNP types, as well as the results

Doxorubicin-loaded human serum albumin nanoparticles overcome transporter-mediated drug resistance in drug-adapted cancer cells

• Hannah Onafuye,
• Sebastian Pieper,
• Dennis Mulac,
• Jindrich Cinatl Jr.,
• Mark N. Wass,
• Klaus Langer and
• Martin Michaelis

Beilstein J. Nanotechnol. 2019, 10, 1707–1715, doi:10.3762/bjnano.10.166

• available in HSA nanoparticles. Doxorubicin sensitivity of the used neuroblastoma cell lines The parental neuroblastoma cell line UKF-NB-3 and its doxorubicin- (UKF-NB-3rDOX20) and vincristine-adapted (UKF-NB-3rVCR1) sub-lines substantially differed in their doxorubicin sensitivity (Figure 2). UKF-NB-3

• , and vincristine-adapted cancer cell lines often display enhanced ABCB1 levels [20][22][23]. Accordingly, UKF-NB-3rVCR1 cells are sensitised by the ABCB1 inhibitor zosuquidar [2][3][4][5][6] to doxorubicin to the level of parental UKF-NB-3 cells (Supporting Information File 1, Figure S1), which

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• of a protein corona. The use of two substantially different serum types further allowed us to assess the effect of the source origin on the protein adsorption. FBS is a common additive in standard cell culture media for many human cell lines and is frequently used as protein source in corona studies

The systemic effect of PEG-nGO-induced oxidative stress in vivo in a rodent model

• Qura Tul Ain,
• Samina Hyder Haq,
• Abeer Alshammari,
• Moudhi Abdullah Al-Mutlaq and
• Muhammad Naeem Anjum

Beilstein J. Nanotechnol. 2019, 10, 901–911, doi:10.3762/bjnano.10.91

• administration and can be cleared gradually by renal and fecal excretion. Furthermore, a number of in vitro studies indicated that treatment of various cell lines, such as 3T3 and Hela, greatly reduced the cellular viability [34]. Due to the contradictory study results, the applicability of PEG-GO for drug

Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy

• Boris N. Khlebtsov,
• Andrey M. Burov,
• Timofey E. Pylaev and
• Nikolai G. Khlebtsov

Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79

• plasmonic photothermal therapy. In this work we utilized fluorescent properties of our nanocomposites to study the folate-mediated nanoparticle uptake. Folate-positive HeLa and folate-negative HEK 293 cell lines were used as models. PEGylated AuNRs-PDA-R123-PEG particles were used as a reference to estimate

Targeting strategies for improving the efficacy of nanomedicine in oncology

• Gonzalo Villaverde and
• Alejandro Baeza

Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16

• specifically to αβ-integrin, which is usually upregulated in many different tumoral cell lines such as breast, lung or fibroblast cancer cells, and also by the epithelial cells of the tumoral blood vessels [32][33]. Ruoshlati et al. have reported that the cyclic version of RGD, CRGDKGPDC (called iRGD), which

• for cell targeting in tumoral cell lines that overexpress nucleolin [40]. The use of targeting moieties provides not only the capacity to the nanoparticles to be selectively engulfed by tumoral cells. It also allows for the localization of the nanocarriers in specific intracellular localizations or

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• manufacturing methods, are yet another problem to be taken into consideration. The aim of this work was to investigate the correlation between the properties of nanoscale hydroxyapatite from different synthesis methods and biological activity represented by the viability of four cell lines: A549, CHO, BEAS-2B

• significantly limits the insight of underlying mechanisms that affect living cells. There is a wide range of available cell lines to study possible organism reactions and cytotoxicity mechanisms, such as endothelial, neural, hepatic, phagocytic or cancer cells [26][27]. Still, systematic studies describing

• different properties of nanopowders and their effect on different cell lines are missing. The aim of this study was to perform a complex, multi-technique physicochemical characterization of ten types of hydroxyapatite nanopowders and to determine their property-dependent influence on living cells. This

Hybrid Au@alendronate nanoparticles as dual chemo-photothermal agent for combined cancer treatment

• Anouchka Plan Sangnier,
• Romain Aufaure,
• Laurence Motte,
• Claire Wilhelm,
• Erwann Guenin and
• Yoann Lalatonne

Beilstein J. Nanotechnol. 2018, 9, 2947–2952, doi:10.3762/bjnano.9.273

• (Figure 3a) with an IC50 equal to 100 µM for both systems whereas Au@HMBP-PEG NPs [39] do not exhibit any cytotoxicity (Supporting Information File 1, Figure S3). Under similar cell-treatment conditions, this IC50 value is consistent with values obtained for free alendronate with other cancer cell lines

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• reactive methyl ester sarcosine derivatives were not yet described as a promising coating of magnetic nanoparticles. The cytotoxic behavior of the nanoparticles with immobilized Dox was investigated in different models of murine and human tumor cell lines and compared with Dox itself and PHPMA-coated

• , various cancer cell lines (Jurkat, K562, HL-60/wt, HL-60/vinc, and B16F10/wt) were treated with the nanoparticles and their cytotoxic activity was compared to that of free Dox (Figure 6). γ-Fe2O3@P(HPMA-MMAA) nanoparticles used in different amounts (2–260 μg depending on cell line) were non-toxic for all

• these cell lines. γ-Fe2O3@P(HPMA-MMAA)-Dox nanoparticles demonstrated slightly higher toxicity (by 5–10%) towards human leukemia cells of Jurkat and HL-60/wt lines compared to free Dox, while drug-resistant HL-60/vinc cells, overexpressing P-glycoprotein, demonstrated a significantly higher sensitivity

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• MCF-7 cells [21]. Herein we report the synthesis of calcium phosphate nanoparticles loaded with 5-FU (CaP@5-FU NPs) with the goal of demonstrating enhanced efficacy in lung and colorectal cancer treatment in cell lines. The simple and reproducible reverse micellar microemulsion method was employed for

• CaP NPs in HCT-15 cell lines (Figure 4C). The same was found in 22.3% and 18.68% of inhibition in A549 and NIH 3T3 cell lines as demonstrated in Figure 4B and Figure 4A, respectively. The comparative low cytotoxicity of CaP NPs as a carrier (even at a higher concentration of 100 µg/mL) reveals the

• (NIH 3T3). We observed an IC50 value of 4.5 µg/mL and 6.5 µg/mL in A549 and HCT-15 cell lines, respectively (Figure 4B,C). Furthermore, upon administration of 5-FU at a concentration of 100 µg/mL, a 67.02% and 81.5% reduction in cell growth in HCT-15 and A549 cell lines was observed. CaP@5-FU NPs

Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants

• Aikaterini-Rafailia Tsiapla,
• Varvara Karagkiozaki,
• Veroniki Bakola,
• Foteini Pappa,
• Panagiota Gkertsiou,
• Eleni Pavlidou and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189

• this nanoplatform cytocompatible. In future studies, the interaction with musculoskeletal tissues will be examined with other cell lines such as mesenchymal stem cells (MSCs) or bone marrow stromal cells (BMSC) in order to evaluate the tissue specific response to our scaffolds. This cytocompatible

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• different from LN-229 cells for all the spice-derived C-dots, results that are in agreement with those described by other researchers on other types of non-cancerous and tumour cell lines using ginger and green tea-based C-dots [27][28]. The fact that the citrate-derived C-dots did not induce any

• glucose, supplemented with 10% FBS and 100 U·mL−1 penicillin/100 μg·mL−1 streptomycin. Both cell lines (passages 5 to 12) were maintained in a humidified atmosphere with 5% CO2, at 37 °C. Cell viability test Cells were seeded in 96-well plates at a density of 10,000 cells per well. On the following day

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• transported between cardiac myocytes using membrane nanotubes [29]. In our study, we demonstrate that MSCs efficiently uptake QDs in serum-free conditions and then excrete the QDs, which then accumulate in MDA-MB-231 and MCF7 breast cancer cell lines in the 3D co-culture (Figure 3C,D). Noteworthy is that the

• experiments. Human breast cancer cell lines MDA-MB-231 (ATCC HTB-26™) and MCF7 (ATCC HTB-22™) were propagated in DMEM supplemented with 10% FBS and penicillin/streptomycin (100 U/mL and 100 μg/mL, respectively) (complete cancer cell medium). The cells were cultured in 25 cm2 polystyrene tissue culture flasks