PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• nucleus is constrained by the pore dimension of the nucleus, because 5 nm gold nanoparticles entered the cell nucleus of human fibroblasts whereas particles larger than 30 nm were retained in the cytoplasm [91]. The silver nanoparticles used in our study have a size of 70 nm and thus, it is understandable

• nanoparticles The increasing use of silver in the form of nanoparticles raises the question whether these compounds are potentially harmful to the health of living organisms in terms of genotoxicity. Experiments were therefore also carried out with Chinese hamster fibroblasts to explore the genotoxic effects of

Carbon-based smart nanomaterials in biomedicine and neuroengineering

• Antonina M. Monaco and
• Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

• interfacing cardiomyocytes with CNTs accelerates cells maturation, resulting in an increased expression of mature phenotype-related genes. Lin and colleagues [64] studied in vitro how pristine SWCNTs dispersed in an extracellular medium can affect the viability of vascular adventitial fibroblasts and their

Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells

• Claudia Strobel,
• Martin Förster and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190

• from the vein of an umbilical cord, the presence of fibroblasts cannot be excluded [30]. Moreover, primary cells are known to change their phenotype with increasing cultivation time [31]. In this context, the assessment of the endothelial phenotype with respect to cultivation time was of interest. The

• ) and nearly no CD90+ cells (fibroblasts, Figure 1b). vWF and CD31 are known to be endothelial [32] and CD90 is a fibroblast cell type specific marker [33]. The experiment was successfully validated using HUVEC from another supplier (provitro GmbH, Germany; Figure 1). Reactivity of CD90 antibody against

• CD90+ human fibroblasts (BJ-cells) was corroborated in a previous experiment (≥99% CD90+ were detected, data not shown). It can be concluded that the HUVEC culture was pure with no alterations of the endothelial phenotype during the experimental setup. This means that the obtained results of the

Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages

• Dagmar A. Kuhn,
• Dimitri Vanhecke,
• Benjamin Michen,
• Fabian Blank,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174

• . Many cell types such as the capillary endothelium, type I epithelial cells, muscle cells as well as fibroblasts, exhibit caveolin-mediated endocytosis, which occurs at the site of the lipid rafts [20][26]. These rafts are plasma membrane regions (subdomains), which consist of glycosphingolipids and

• observed in several cell types including capillary endothelium, type I alveolar epithelial cells, smooth muscle cells and fibroblasts [20]. Therefore, this result supports the cell type specific mechanism of this uptake. The colocalization of clathrin heavy chain and the 40 nm PS NP fluorescence in the

Current state of laser synthesis of metal and alloy nanoparticles as ligand-free reference materials for nano-toxicological assays

• Christoph Rehbock,
• Jurij Jakobi,
• Lisa Gamrad,
• Selina van der Meer,
• Daniela Tiedemann,
• Ulrike Taylor,
• Wilfried Kues,
• Detlef Rath and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2014, 5, 1523–1541, doi:10.3762/bjnano.5.165

• nanoparticles in toxicity assays with bacteria as well as mammalian fibroblasts and gametes reveal distinctive correlations between particle composition and toxic effects. Due to the high purity of the material the influence of additional surface ligands could be systematically studied. High purity of these

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• targeted drug and gene delivery [29][30][31]. Hence, MTT assays were used to study the cell viabilities of fibroblasts and cancer cells after treatment with AuNPs to check the cytotoxicity and the anticancer properties of the AuNPs for their future biomedical applications. Results and Discussion Synthesis

• also observed in the UV–vis studies for the blank proteins (Figure S3, Supporting Information File 1). Cell viability after exposure to AuNPs To investigate the cell viability after the exposure to AuNPs, MTT assays were conducted with mouse embryonic fibroblasts (NIH-3T3) by treating them with AuNPs

• -AuNPs, BHG-AuNPs and BGG-AuNPs. All of these AuNPs had zeta potentials close to zero; hence their IC50 values were also similar. The IC50 values of the different protein-coated AuNPs followed the order OVA > BSA > BHG > LYS > BGG > HIS for the NIH-3T3 fibroblasts. To study further the role of the

Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

• Sören B. Gutekunst,
• Carsten Grabosch,
• Alexander Kovalev,
• Stanislav N. Gorb and
• Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152

• ; Introduction The adhesion of many cell types, including fibroblasts, myocytes, and neurons, depends on the mechanical properties of the cellular microenvironment [1][2][3]. In particular, cells prefer to adhere to materials, which have mechanical properties similar to the ones found in their natural biological

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• filaments with the use of different types of cytochalasin, Rotsch and Radmacher have reported a decrease of the Young’s modulus of fibroblasts [16]. Similar findings regarding the role of actin filaments have been reported in other types of cells such as lymphocyte and Jurkat cells [19]. The role of the

• dynamically different types of MT configurations (unstable and stable) and intermediate filaments (IF), which all act to impart a distinct cellular type of transient metastability. On the other hand, it has been reported that the disassembly of the MT of fibroblasts by using colchicine and colcemide does not

Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats

• Nadine Haberl,
• Stephanie Hirn,
• Alexander Wenk,
• Jörg Diendorf,
• Matthias Epple,
• Blair D. Johnston,
• Fritz Krombach,
• Wolfgang G. Kreyling and
• Carsten Schleh

Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105

• oxygen species (ROS) in human lung fibroblasts in a dose-dependent manner [19]. In contrast, very little is known about the cytotoxic and proinflammatory effects of AgNP in the respiratory system in vivo [20]. Intratracheal instillation of slightly agglomerated AgNP in mice resulted in progressively

Nanolesions induced by heavy ions in human tissues: Experimental and theoretical studies

• Marcus Bleicher,
• Lucas Burigo,
• Marco Durante,
• Maren Herrlitz,
• Michael Krämer,
• Igor Mishustin,
• Iris Müller,
• Francesco Natale,
• Igor Pshenichnov,
• Stefan Schramm,
• Gisela Taucher-Scholz and
• Cathrin Wälzlein

Beilstein J. Nanotechnol. 2012, 3, 556–563, doi:10.3762/bjnano.3.64

• fibroblasts. Cells were irradiated with Au ions (energy: 8 MeV/n, linear energy transfer (LET): 13000 keV/μm; fluence: 3·106 ions/cm2) at a low angle and fixed after 1 h. H4K16ac (green) is increased at damage sites. DNA damage is shown by γH2AX staining (red). DNA is counterstained with ToPro3 (blue). From