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Search for "in vitro studies" in Full Text gives 61 result(s) in Beilstein Journal of Nanotechnology.
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
- nanoparticles over time may induce secondary effects, which cannot be ruled out by such in vitro studies. Chronic exposure and inhalation studies need to address this issue in the future. As summarized in Table 2, the effects induced by the PVP-coated silver nanoparticles in three different lung systems, i.e
Carbon-based smart nanomaterials in biomedicine and neuroengineering
Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196
- . These differences can induce distinct toxicological responses in biological systems and require a systematic investigation. In vitro studies, carried out on human cell lines (i.e., HepG2, BEAS-2B, PC12, hMSCs), have demonstrated that the cyto- and genotoxicity of graphene depends on the dose, shape, and
The surface properties of nanoparticles determine the agglomeration state and the size of the particles under physiological conditions
Beilstein J. Nanotechnol. 2014, 5, 1774–1786, doi:10.3762/bjnano.5.188
- physiological media is possible. As a result, it is shown that differences in the agglomeration state and therefore in size were observed under physiological conditions both in presence and in absence of serum proteins. This is especially relevant with regard to in vitro studies. Characterization techniques As
- physiological salinity. The high stability of silica NPs can be attributed to a "gel-like" layer on the surface of the particles, which is discussed in the literature as sterically stabilizing coating [81]. With regard to in vivo and in vitro studies, NexSil20 was also characterized in the presence of serum
In vitro and in vivo interactions of selected nanoparticles with rodent serum proteins and their consequences in biokinetics
Beilstein J. Nanotechnol. 2014, 5, 1699–1711, doi:10.3762/bjnano.5.180
- AuNP in mouse serum after 15 min of incubation. Figure 5 shows the very good reproducibility of two independent LC–MS/MS measurements for each of all five AuNP. Biokinetics of AuNP after administration via three routes of intake The above mentioned in vitro studies were designed to provide a better
- of the PEG shell. Our in vitro studies clearly demonstrated the rapid binding of serum proteins to AuNP. This is likely reflected in the in vivo biokinetics results found after intravenous injection which led to a predominant accumulation in the liver and Kupffer cell endocytosis. Yet, the fact that
- translocation across membrane barriers by either transcellular endo- and exocytosis, or by paracellular transport mechanisms. While there are some in vitro studies suggesting protein exchange on NP surfaces during membrane crossing we are not aware of any in vivo studies having shown such exchange [17][18][19
Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure
Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171
- nanoparticles (NPs) in the lung by in vitro studies are usually performed under submerged conditions where NPs are suspended in cell culture media. However, the behaviour of nanoparticles such as agglomeration and sedimentation in such complex suspensions is difficult to control and hence the deposited cellular
- dose rates differ, but also the distribution of the NPs within the lung and the clearance are different. As in our in vitro studies these two confounding factors are eliminated, the dose rate still remains an important determinant to possibly explain the differences in toxicity when exposing cells at
In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?
Beilstein J. Nanotechnol. 2014, 5, 1477–1490, doi:10.3762/bjnano.5.161
- has to be assessed. In the context of this review we have focused on in vitro studies. The advantage of such studies is the easy screening capability and the possibility to monitor in detail biomolecular pathways and changes in gene expression as a measure of a possible biologically adverse response
Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149
- reflects a more realistic scenario for in vitro studies than addition of NPs in suspension. Our results indicated a significant difference between the two exposure methods with submerged cultures showing a stronger effect than ALI exposed cells and, thus, revealed the importance of an adequate experimental
Optimizing the synthesis of CdS/ZnS core/shell semiconductor nanocrystals for bioimaging applications
Beilstein J. Nanotechnol. 2014, 5, 919–926, doi:10.3762/bjnano.5.105
- -encapsulated nanoparticles under various pH values, testing the stability of the F127-CdS/ZnS QDs. The variation of the hydrodynamic diameter under different pH values is shown in Figure 9. The result suggests that their stability is not affected by pH values. Cell imaging and viability studies For in vitro
- studies, a cell viability (MTS) assay was carried out for F127-CdS/ZnS QDs. As shown in Figure 10, we tested the cell viability of Panc-1 cells, treating them with various concentrations of ternary nanocrystal formulations for 48 h. The cell viability remained at 84% even at a concentration as high as 500
Biocalcite, a multifunctional inorganic polymer: Building block for calcareous sponge spicules and bioseed for the synthesis of calcium phosphate-based bone
Beilstein J. Nanotechnol. 2014, 5, 610–621, doi:10.3762/bjnano.5.72
- osteoporosis [19]. Recently, we could show in in vitro studies, by using SaOS-2 cells growing in calcium bicarbonate-deprived medium that these cells respond with a significant increase in calcium deposit formation after exposure to bicarbonate [20]. The cells start to form larger crystallite nodules on their
Near-infrared dye loaded polymeric nanoparticles for cancer imaging and therapy and cellular response after laser-induced heating
Beilstein J. Nanotechnol. 2014, 5, 313–322, doi:10.3762/bjnano.5.35
- was evaluated by using a Cary WinUV spectrophotometer (Varian/Agilent Technologies, Switzerland). In vitro studies of NPs Cancer cells MES-SA, Dx5, and SKOV3 were purchased from American Type Culture Collection (Manassas, VA) along with McCoy’s 5A medium and fetal bovine serum. Cell culture supplies
- after background subtraction). The ratio R was then determined by normalizing the total pixel intensity of this region of interest to its total area. HT treatment Two different heating modes, namely (1) an incubator and (2) a laser/NP HT delivery system, were used for in vitro studies. Detailed
Cytotoxic and proinflammatory effects of PVP-coated silver nanoparticles after intratracheal instillation in rats
Beilstein J. Nanotechnol. 2013, 4, 933–940, doi:10.3762/bjnano.4.105
- be able to cross the air-blood-barrier (ABB), towards circulation, and accumulate in secondary organs [16][17]. Some in vitro studies have demonstrated toxic effects of AgNP on lung cells: In vitro incubation of a rat alveolar macrophage cell line with AgNP induced a concentration- as well as a size
- metabolization of silver ions. Thus, the dissolution of AgNP and release of silver ions as well as the subsequent biochemical transformations are an important issue in AgNP toxicity [27]. However, most of the information available about the mechanisms of AgNP toxicity has been derived from in vitro studies. The
- were not assessed in these studies. Several in vitro studies dealt with the mechanism of cytokine induction after AgNP exposure. Incubation of human mesenchymal stem cells and of peripheral blood monocytes with the same PVP-AgNP that were used in the present study induced a concentration-dependent
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