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Search for "laser scanning microscopy" in Full Text gives 57 result(s) in Beilstein Journal of Nanotechnology.
Effect of silver nanoparticles on human mesenchymal stem cell differentiation
Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214
- with specific organelle markers. Laser scanning microscopy and phase-contrast microscopy were performed in parallel on identical cell areas (Figure 1A). In cells cultured in the presence of Ag-NPs, agglomerated nanoparticles were visible in a region close to the cell nucleus but not in the cell culture
- uptake by laser scanning microscopy (LSM) LSM was performed to demonstrate the occurrence of intracellular silver nanoparticles in hMSCs after incubation. Therefore, hMSCs were subconfluently grown on 2-well Lab-TekTM glass chamber slides (Thermo Fisher Scientific, Langenselbold, Germany) and
- both ionic and nanoparticulate silver on the differentiation of human mesenchymal stem cells (hMSCs) into adipogenic, osteogenic and chondrogenic lineages and on the secretion of the respective differentiation markers adiponectin, osteocalcin and aggrecan. Results: As shown through laser scanning
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
- scanning microscopy) are other techniques that permit to visualize nanoparticles in cells. Therefore, hMSC were cultured in the presence of either 20 µg mL−1 silver (as nanoparticles) or 2 µg mL−1 silver ions (as silver acetate; control to separate the nanoparticle and the ion effect) at 37 °C for 24 h
- dissolution process in cells (including the localization of low concentrations of small nanoparticles as well as silver ions) imaging at the Ag L3,2 edges is a promising option for future work. Focused ion beam (FIB) and optical microscopy (phase contrast microscopy; fluorescence microscopy; confocal laser
Imaging the intracellular degradation of biodegradable polymer nanoparticles
Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201
- ., number of detached magnetite crystals, and the number of nanoparticles in one endosome), we demonstrate the importance of TEM studies for such applications in addition to fluorescence studies (flow cytometry and confocal laser scanning microscopy). Keywords: biodegradation; mesenchymal stem cells; PLLA
- period of 14 days, primarily by means of transmission electron microscopy (TEM), in order to demonstrate their degradation. Furthermore, confocal laser scanning microscopy (CLSM) and flow cytometry were used to monitor the nanoparticle load of individual cells. As a probe we chose tailor-made PLLA
- cultures. All values are triplicates with the error bars representing the standard deviation. Confocal laser scanning microscopy (CLSM) Confocal laser scanning microscopy (CLSM) was applied to demonstrate the intracellular distribution of nanoparticles over the period of 14 days. As described in [26], for
Different endocytotic uptake mechanisms for nanoparticles in epithelial cells and macrophages
Beilstein J. Nanotechnol. 2014, 5, 1625–1636, doi:10.3762/bjnano.5.174
- presence of the endocytotic proteins which are involved in endocytosis in both cell types (Figure 2). To achieve this, laser scanning microscopy (LSM) was applied as the primary tool for this investigations. Flotillin-1 and clathrin heavy chain could be visualized in J774A.1 cells, but caveolin-1 was not
- fluospheres (molecular probes) were used at a concentration of 20 µg/mL in RPMI. Laser scanning microscopy of fixed and living cells For LSM imaging, the cells were fixed with 3% paraformaldehyde (PFA, Sigma-Aldrich, Switzerland) in PBS for 15 minutes at room temperature. The cells were then washed with 1
- . Laser scanning microscopy imaging revealed particle uptake in J774A.1 and A549 cells. (A–C) Uptake of 40 nm PS NPs (NP: red, cytosol: grey). (A) Untreated cells with 40 nm NPs. (B) 40 nm NPs and cytochalasin D (cytoD) in J774A.1 and chlorpromazine (cpz) in A549 cells. (C) 40 nm NPs and
Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches
Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149
- , resulting in increased surface concentrations of 1.7, 3.4, and 5.1 µg Ag/cm2. Therefore, the two exposure scenarios could be compared due to similar mass deposition on the lung cells surface. Cell morphology and particle uptake The cell morphology was studied with laser scanning microscopy (LSM) (Figure 2
- ; Sigma-Aldrich) served as positive control to induce the release of TNF-α and IL-8, respectively. Laser scanning microscopy As described in [44], the triple cell co-cultures were fixed on the cell culture insert with 3% paraformaldehyde in phosphate buffered saline (PBS) for 15 min at room temperature
Dry friction of microstructured polymer surfaces inspired by snake skin
Beilstein J. Nanotechnol. 2014, 5, 1091–1103, doi:10.3762/bjnano.5.122
- elevated, so the snake can generate propulsion due to the interlocking of its microstructure with surface asperities. The results of the study of the snake skin’s microstructure by using atomic force microscopy (AFM) and confocal laser scanning microscopy (CLSM) showed that the anisotropic geometry of the
Fibrillar adhesion with no clusterisation: Functional significance of material gradient along adhesive setae of insects
Beilstein J. Nanotechnol. 2014, 5, 837–845, doi:10.3762/bjnano.5.95
- revealed by confocal laser scanning microscopy (CLSM). This gradient is hypothesized to be an evolutionary optimization enhancing adaptation of adhesive pads to rough surfaces, while simultaneously preventing setal clusterisation. Such an optimisation presumably increases the performance of the adhesive
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