Search results
Search for "phosphate" in Full Text gives 331 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.
Electrochemical nanostructured CuBTC/FeBTC MOF composite sensor for enrofloxacin detection
Beilstein J. Nanotechnol. 2024, 15, 1522–1535, doi:10.3762/bjnano.15.120
- voltammogram in ENR-free blank solution with no electrochemical response for ENR. In phosphate-buffered saline (PBS) containing ENR, there is a peak at 0.85 V on all four electrodes, attributed to the oxidation of ENR during the anodic scanning. No reduction peak was observed, revealing that the
- %), and HCl (37%) were bought from Fisher and Sigma-Aldrich. All chemicals and reagents were of analytical grade and used without further purification. K3[Fe(CN)6] (≥99.5%) and K4[Fe(CN)6] (≥99.5%) were purchased from China. Phosphate-buffered saline (PBS) was prepared by mixing 0.2 M KH2PO4 and 0.2 M
- K2HPO4 in double-distilled water. The pH of the solution used for the optimization of electrolyte solution was changed from 6.0 to 9.0 by controlling the amount of these phosphate salts. Enrofloxacin stock solution was prepared by dissolving ENR powder in double-distilled water. The ENR solutions with
Dual-functionalized architecture enables stable and tumor cell-specific SiO2NPs in complex biological fluids
Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100
- equipment (Malvern Instruments Ltd., UK – detection angle of 173° and laser wavelength of 633 nm). For DLS measurements, NPs were dispersed in MilliQ water (1.0 mg·mL–1). To determine the zeta potential, the NPs were diluted in 10 Mm of phosphate buffer at a concentration of 1.0 mg·mL–1. All measurements
- penicillin and streptomycin (Sigma-Aldrich) and ii) human plasma. The human plasma was in compliance with ethics in research committee (CEP) of UNICAMP/Brazil. Particularly, the plasma was diluted (final percentage of 5.5%) in phosphate-buffered saline (PBS, pH 7.4, 10 mM) for incubation with particles. The
Synthesis, characterization and anticancer effect of doxorubicin-loaded dual stimuli-responsive smart nanopolymers
Beilstein J. Nanotechnol. 2024, 15, 1189–1196, doi:10.3762/bjnano.15.96
- their transition to the active phase of reproduction. The nanomaterials were added to the cell suspension in the wells. For this, complexes of DOX-SNPs were prepared as described in section “Preparation of DOX-SNPs” to equivalents of loaded DOX of 1, 3, and 5 µmol/L in 0.05 mol/L phosphate-buffered
- structure with a low carrying capacity may not provide the desired effect. DOX loading of SNPs was conducted as previously mentioned in section “Preparation of DOX-SNPs”. The DOX-loading capacity of smart nanopolymers was investigated in the concentration range of 1–9 µM (5 mg SNPs in 5 mL 0.05 M phosphate
Recent updates in applications of nanomedicine for the treatment of hepatic fibrosis
Beilstein J. Nanotechnol. 2024, 15, 1105–1116, doi:10.3762/bjnano.15.89
- liver cell types, there is a particular receptor that could be targeted to specifically deliver antifibrotic drugs, as illustrated in Figure 2b. On the surface of HSCs, the mannose 6-phosphate receptor (M6PR) is probably the most prominent receptor for targeting HSCs. This particular molecule is
- binding protein. This protein is not available in hepatocytes, thus enabling specific targeting to activated HSCs in liver fibrosis [72]. Tan and co-workers also constructed albumin–mannose 6-phosphate-modified solid lipid NPs to deliver matrine to HSCs [73]. The active substance matrine was first loaded
- into the solid lipid NPs using the microemulsion-probe ultrasound method, while mannose 6-phosphate was conjugated to albumin. The mannose 6-phosphate-conjugated albumin was then decorated onto the surface of matrine-loaded solid lipid NPs, and its HSC-targeting efficiency was evaluated in vitro and in
Unveiling the potential of alginate-based nanomaterials in sensing technology and smart delivery applications
Beilstein J. Nanotechnol. 2024, 15, 1077–1104, doi:10.3762/bjnano.15.88
- controlled amygdalin release, with an increased cytotoxic effect on cancerous cells while sparing normal cells and tissues. Alginate nanoparticles can be applied to ocular DDSs [91]. Kianersi and coworkers investigated alginate-based nanoparticles for betamethasone sodium phosphate delivery in the human eye
Therapeutic effect of F127-folate@PLGA/CHL/IR780 nanoparticles on folate receptor-expressing cancer cells
Beilstein J. Nanotechnol. 2024, 15, 954–964, doi:10.3762/bjnano.15.78
- ligand folate to improve their capacity to target cells expressing the folate receptor. Materials and Methods Chlorambucil (C0253), PLGA 504H (719900), IR780 (425311), Pluronic™ F-127 (P2443), poly(vinyl alcohol) (PVA) (P8136), phosphate buffered saline (PBS) (P4417), dimethyl sulfoxide (DMSO) (472301
Electrospun nanofibers: building blocks for the repair of bone tissue
Beilstein J. Nanotechnol. 2024, 15, 941–953, doi:10.3762/bjnano.15.77
- of calcium and phosphate and the protection of bone marrow are among the characteristics of bone tissue [9][10]. Bone tissue, which is a special form of connective tissue, consists of cells, fibers, and extracellular matrix; histologically, it can be divided into two bone types, namely, woven bone
- -based nanofiber scaffolds for tissue regeneration (Stellenbosch Nanofiber Company, South Africa) [45]. ReBOSSIS consists of β-tricalcium phosphate, PLGA, and silicon-doped calcium carbonate to support bone formation. ReBOSSIS electrospun fibers are distinguished from other market products by a cotton
The effect of age on the attachment ability of stick insects (Phasmatodea)
Beilstein J. Nanotechnol. 2024, 15, 867–883, doi:10.3762/bjnano.15.72
- . Freshly molted adult and subadult individuals were acquired from laboratory stock and used for imaging as well. Three individuals were chosen for each age group. Tarsi were cut off at the tarso-tibial joint and transferred into 1.5 mL solution of phosphate-buffered saline (PBS) and Triton TM-X100 (Sigma
Electrospun polysuccinimide scaffolds containing different salts as potential wound dressing material
Beilstein J. Nanotechnol. 2024, 15, 781–796, doi:10.3762/bjnano.15.65
- , USA); Dulbecco's modified Eagle medium (DMEM, Gibco, USA); MEM, no glutamine, no phenol red (Gibco, USA); phosphate-buffered saline (Gibco, USA); fetal bovine serum (FBS, Gibco, USA); penicillin/streptomycin mix (Gibco, USA); ʟ-glutamine (Gibco, USA); non-essential amino acid solution (NEAA, Gibco
- was removed from each well and 200 µL of phosphate-buffered saline was added to eliminate the residual media as well as cells that were either loosely attached or did not attach to the plate. Then, 100 µL of diluted WST-1 reagent was added to the cells and incubated for 4 h. The absorbance values of
Gold nanomakura: nanoarchitectonics and their photothermal response in association with carrageenan hydrogels
Beilstein J. Nanotechnol. 2024, 15, 678–693, doi:10.3762/bjnano.15.56
- DTAB-AuNM, respectively, over a wavelength range 300–900 nm. Stability of AuNMs (CTAB-AuNM, MTAB-AuNM, and DTAB AuNM) in different media at the ratio (NPs:media) 3:1 where (a), (b), and (c) represent the stability of AuNMs in ethanol (70%), NaCl (0.9%), and 1X phosphate buffer saline (PBS). (a) k-CG
Comparative analysis of the ultrastructure and adhesive secretion pathways of different smooth attachment pads of the stick insect Medauroidea extradentata (Phasmatodea)
Beilstein J. Nanotechnol. 2024, 15, 612–630, doi:10.3762/bjnano.15.52
- tarsomeres were fixed in 2.5% glutaraldehyde in (pH 7.4) phosphate-buffered saline (PBS) for 24 h, washed two times in PBS for 30 min each, fixed in 1% aqueous OsO4 for 1 h, and washed two times in double-distilled water, for 30 min each. After fixation, the samples were dehydrated using an ascending ethanol
Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent
Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46
- 700 mg TPA was isolated from each culture batch. The reproducibility of each TPA extract composition was routinely screened by phosphate content [75] and electrospray-ionization mass spectrometry [76] and ordered according to decrescent abundance as archaeol analog methyl ester of
- Bligh and Dyer method [77] by a colorimetric phosphate microassay [75]. Size and ζ potential of nanovesicles were determined by dynamic light scattering and phase analysis light scattering, respectively, using a Zetasizer Nano ZS apparatus (Malvern Instruments Ltd, UK). To prepare RhPE-labeled
- nanoarchaeosome (nanoARC-Chol (ALN)). (B) Structure of phosphatidylglycerol phosphate methyl ester (PGP-Me). (C) Structure of the four polar archaeolipids composing the total polar lipids from H. tebenquichense. HUVEC viability upon nanovesicle uptake. Viability upon 24 h of incubation with (A) void nanovesicles
Fabrication of nanocrystal forms of ᴅ-cycloserine and their application for transdermal and enteric drug delivery systems
Beilstein J. Nanotechnol. 2024, 15, 465–474, doi:10.3762/bjnano.15.42
- formulation. The pH stability test of DCS We prepared two solutions for pH stability test to simulate the gastrointestinal environment: 0.1 M of an HCl solution (pH 1.2 at the stomach) (Sigma-Aldrich) and 0.1 M of phosphate buffer (pH 7.4 at the terminal ileum) (Sigma-Aldrich) [26]. Approximately 5 mg of DCS
Vinorelbine-loaded multifunctional magnetic nanoparticles as anticancer drug delivery systems: synthesis, characterization, and in vitro release study
Beilstein J. Nanotechnol. 2024, 15, 256–269, doi:10.3762/bjnano.15.24
- ), and thiol-polyethylene glycol (SH-PEG, purity: ≥95%), ammonium hydroxide (NH4OH, 28–30%), phosphate-buffered saline (PBS) were purchased from Merck. The chemical vinorelbine tartrate CRS (European Pharmacopoeia (EP) Reference Standard, catalog no: Y0000463) was also acquired from Merck. Synthesis of
- ) were combined with 25 mL of phosphate solution (pH 8.5). The purpose of this mixture was to facilitate the loading of vinorelbine tartrate into the PDA/Fe3O4 content. Subsequently, 25 mg of VNB was added to the prepared mixture, and the solution was thoroughly mixed for 5 h. The resulting nanostructure
- cycles [41]. Determination of vinorelbine drug release VNB/PDA/Fe3O4 NPs (at ratios of 1:1, 2:1, and 4:1) were placed into dialysis capsules at a concentration of 1 mg/mL. Subsequently, each prepared dialysis capsule was placed in 100 mL phosphate solution at pH 5.5 and 7.4 [30]. The experiment was
Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics
Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17
- -127 (P2443), poly(vinyl alcohol) (PVA) (P8136), phosphate buffered saline (PBS) (P4417), dimethyl sulfoxide (DMSO) (472301), dichloromethane (DCM), 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl-2H-tetrazolium bromide (MTT) (M2128), and coumarin-6 (442631), K3[Fe(CN)6] (1049730100) were purchased from
Assessing phytotoxicity and tolerance levels of ZnO nanoparticles on Raphanus sativus: implications for widespread adoptions
Beilstein J. Nanotechnol. 2024, 15, 115–125, doi:10.3762/bjnano.15.11
- nanomaterials may dissolve and then undergo biotransformation or be internalized as intact particles in a biological context [48]. As an example, the biotransformation of ZnO NPs into Zn nitrate, Zn phosphate, and Zn citrate in desert plant species has been reported [49]. Also, the bioaccumulation of intact ZnO
- ) were separately homogenized using a mortar and pestle with 10 mL of phosphate buffer (50 mM at pH 7.4). The mixture was centrifuged at 9000 rpm for 15 min at 4 °C, the supernatant was collected, and the soluble protein content was determined according to the Bradford’s method [57]. Determination of IAA
Development and characterization of potential larvicidal nanoemulsions against Aedes aegypti
Beilstein J. Nanotechnol. 2024, 15, 104–114, doi:10.3762/bjnano.15.10
- conditions. Modified Franz cells, equipped with a polyethersulfone membrane (Sigma-Aldrich) and with a diffusion area of 1.77 cm2 were used in the assays. A Microette (Hanson Research, USA) was used. The receptor compartment was filled with 7.0 mL of a receptor solution composed of 0.1 M phosphate buffer and
Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface
Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8
- surrogate. FITC-labelled sheep polyclonal antibody against BSA was purchased from Thermo Fisher Scientific Inc. The blocking buffer was phosphate-buffered saline (PBS) with casein (Pierce). Solvents and additives were purchased from Aldrich (Spain). PBS was purchased from Fisher Scientific. Nanofiber
Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells
Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100
- may result from the positive charges carried by RhBITC at physiological pH, which are absent in the FITC molecules. The positive charges are expected to participate in attractive electrostatic interactions with the negatively charged bacterial membrane, that is, the phosphate groups of their
Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment
Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96
- Materials, Inc. (OH, USA), respectively. Dimethylacetamide (Alfa Aesar), phosphate-buffered saline (PBS), sera, rhodamine–phalloidin (RP), 4′,6-diamidino-2-phenylindole (DAPI), and Alexa Fluor 488–beta-III tubulin antibody (AF488-anti-β3 tubulin) were obtained from Thermo Fisher Scientific (MA, USA). RPMI
Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study
Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93
- phosphate-buffered saline (PBS), pH 7.4, were purchased from Bio basic (Toronto, Canada). Dulbecco’s modified Eagle’s medium, Ham’s F-12 nutrient mixture (DMEM/F12), and fetal bovine serum (FBS) were purchased from Gibco-BRL Life Technologies (Gibco–Thermo Fisher Scientific, Waltham, MA, USA). Fluorescein
Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one
Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85
- chloride was purchased from B. Braun SE, Melsungen, Germany. Phosphate-buffered saline (PBS) 10-fold solution was purchased from Fisher scientific, Fair Lawn, New Jersey, USA. Human serum albumin (Plasbumin®20) was purchased from Grifols Deutschland GmbH, Frankfurt am Main, Germany. Trypan blue 0.4
Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes
Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73
- promastigotes (control and treated cells) were washed in phosphate-buffered saline (PBS) pH 7.2 and adhered for 10 min on glass coverslips previously coated with poly-ʟ-lysine (Sigma-Aldrich, Germany). The intracellular amastigotes were obtained after infection of RAW 264.7 macrophages at a ratio of ten
Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies
Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66
- , and NLC-BNZ (0, 15, 31, 62, and 125 μg·mL−1). The MTT reagent (5 mg·mL−1 in phosphate-buffered saline (PBS)) was then added for 3 h. Dimethyl sulfoxide (DMSO,100 µL per well) was added under agitation for 10 min to dissolve the MTT. The color was measured in a microplate reader (MultiskanTM GO
Carboxylic acids and light interact to affect nanoceria stability and dissolution in acidic aqueous environments
Beilstein J. Nanotechnol. 2023, 14, 762–780, doi:10.3762/bjnano.14.63
- ]. Nanoceria was partially biotransformed in cucumber plants to cerium phosphate within the roots and to cerium carboxylates in the shoots, presumably aided by carboxylic acids excreted by the roots [17]. Ceria transformation within cucumber plants was also found to be affected by phosphate, with a higher
- percentage of cerium carboxylates in the shoots in the absence of added phosphate [18]. Nanoceria can be taken up by food crops; however, limited biotransformation was observed in soil cultivated soybeans [19]. Coated and uncoated ceria nanoparticles were found in the roots and shoots of corn plants. The
- observed in organic solvents [36]. Partially degraded nanoceria formed cerium phosphate within rats and mice, presumably by a dissolution/re-crystallization process [37][38][39][40]. In artificial soil solutions, partial dissolution was observed at pH 4, however, not at pH 7 or pH 9 over 28 days [41]. The
Other Beilstein-Institut Open Science Activities
