Detection of interaction between biomineralising proteins and calcium carbonate microcrystals

• Hanna Rademaker and
• Malte Launspach

Beilstein J. Nanotechnol. 2011, 2, 222–227, doi:10.3762/bjnano.2.26

• . These two protein bands (one at approx. 19 kDa and the other at about 25 kDa) belong to the protein perlucin [10]. The corresponding fractions of purified protein showing these two bands on SDS-PAGEs had been subjected to N-terminal amino acid sequence analysis [11] in several different experiments

• of perlucin has saccharide oligomers bound to these sites. In MALDI-MS (matrix-assisted laser desorption/ionization mass spectroscopy) of perlucin [10] variable molecular weights of perlucin are detectable, which can be related to a 10 amino acid repeat at the C-terminus being present in various

Electrochemical behavior of dye-linked L-proline dehydrogenase on glassy carbon electrodes modified by multi-walled carbon nanotubes

• Haitao Zheng,
• Leyi Lin,
• Yosuke Okezaki,
• Ryushi Kawakami,
• Haruhiko Sakuraba,
• Toshihisa Ohshima,
• Keiichi Takagi and
• Shin-ichiro Suye

Beilstein J. Nanotechnol. 2010, 1, 135–141, doi:10.3762/bjnano.1.16

• typical Michaelis–Menten catalytic response with lower apparent constant. Keywords: dye-linked L-proline dehydrogenase; electrocatalysis; electron transfer; multi-walled carbon nanotube; Introduction As an essential amino acid for the proper functioning of tendons and joints in the human body, the quick

• for the detection of various types of chemical or biochemical substances [18][19][20][21][22][23]. Some amino acid biosensors have already been reported based on CNTs-modified electrodes [24][25][26][27]. In our previous work, the recombinant thermostable dye-linked L-proline dehydrogenase (L-proDH