Macromolecular shape and interactions in layer-by-layer assemblies within cylindrical nanopores

• Thomas D. Lazzara,
• K. H. Aaron Lau,
• Wolfgang Knoll,
• Andreas Janshoff and
• Claudia Steinem

Beilstein J. Nanotechnol. 2012, 3, 475–484, doi:10.3762/bjnano.3.54

• molecular recognition of biotinylated-bovine serum albumin (b-BSA) by avidin. Avidin has four biotin-binding sites, whereas the b-BSA used has 13 biotin molecules per protein on average. Avidin with a mass of MW = 66–69 kDa, and which is positively charged at neutral pH, was first adsorbed onto the

• Calbiochem (purity 12.9 units/mg). Biotinylated-bovine serum albumin (b-BSA) with 13 mol biotin/mol albumin, poly(sodium 4-styrenesulfonate) (PSS) (Mw = 70 kDa), poly(allylamine hydrochloride) PAH (Mw = 50–65 kDa), CuCl2, NaCl, and 16-mercaptohexadecanoic acid (90%) were purchased from Sigma Aldrich (St

• serum albumin was compared with that of linear polyelectrolytes (linear-PEs), both species being of similar molecular weight. LbL deposition within the cylindrical AAO geometry for different pore diameters (d0 = 25–80 nm) for the various macromolecular species, showed that the multilayer film growth was

Analysis of fluid flow around a beating artificial cilium

• Mojca Vilfan,
• Gašper Kokot,
• Andrej Vilfan,
• Natan Osterman,
• Blaž Kavčič,
• Igor Poberaj and
• Dušan Babič

Beilstein J. Nanotechnol. 2012, 3, 163–171, doi:10.3762/bjnano.3.16

• diameter 55 nm [24]) in water. To prevent aggregation of the beads, we coated them with BSA (bovine serum albumin), 10 mg/mL, for 4 h in an ultrasonic bath. One end of the assembled chain was attached to the surface through prefabricated ferromagnetic-nickel anchoring sites. The nickel dots were

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• bovine serum albumin (BSA) protein as a control did not elicit any change in T2 (Figure 5b). More recently, MRSw biosensors, capable of detecting soluble tumor biomarker proteins (such as CA-125, VEGF, and α-fetoprotein) were described, and used for parallel detection of multiple markers in blood samples