Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure

• Alicja Panas,
• Andreas Comouth,
• Harald Saathoff,
• Thomas Leisner,
• Marco Al-Rawi,
• Michael Simon,
• Gunnar Seemann,
• Olaf Dössel,
• Sonja Mülhopt,
• Hanns-Rudolf Paur,
• Susanne Fritsch-Decker,
• Carsten Weiss and
• Silvia Diabaté

Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171

• reasons for the different sensitivity remain unknown. Future studies need to address the relevance of changes in the dose rate as a critical parameter for cellular toxicity. Moreover, more detailed analysis of particle uptake (e.g., by confocal microscopy or TEM) under ALI and submerged conditions will be

The cell-type specific uptake of polymer-coated or micelle-embedded QDs and SPIOs does not provoke an acute pro-inflammatory response in the liver

• Markus Heine,
• Alexander Bartelt,
• Oliver T. Bruns,
• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Ludger Scheja,
• Christian Waurisch,
• Alexander Eychmüller,
• Rudolph Reimer,
• Horst Weller,
• Peter Nielsen and
• Joerg Heeren

Beilstein J. Nanotechnol. 2014, 5, 1432–1440, doi:10.3762/bjnano.5.155

• apolipoprotein E for the uptake of QDs-micelles into liver cells (Figure 5). In order to visualize sinusoids of the unfixed liver, we performed confocal microscopy using the reflection mode and marked the liver sinusoids by dashed lines. In wild type mice, QDs-micelles (red) were detected with star-shaped cells

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• . The successful incorporation of partly fluorescently labelled integrin αIIbβ3 into the GUVs was confirmed by confocal microscopy. Binding experiments of integrin-GUVs on surfaces and quantum dots coated with RGD ligands revealed that the incorporated integrins were biologically active. In reflection

Optimizing the synthesis of CdS/ZnS core/shell semiconductor nanocrystals for bioimaging applications

• Li-wei Liu,
• Si-yi Hu,
• Ying Pan,
• Jia-qi Zhang,
• Yue-shu Feng and
• Xi-he Zhang

Beilstein J. Nanotechnol. 2014, 5, 919–926, doi:10.3762/bjnano.5.105

• pancreatic cancer. In our study, in vitro confocal microscopy studies were performed to re-confirm the uptake of F127-CdS/ZnS QDs bioconjugates in Panc-1 cells. Panc-1 cells were treated with F127-CdS/ZnS QDs for 2 h. Subsequently, the cells were rinsed with a PBS buffer to remove free nanocrystals and

• imaged by using confocal microscopy. Figure 11 shows robust cellular uptakes of the ternary nanocrystal samples. The red singnal is from F127-CdS/ZnS QDs, and background fluorescence and autofluorescence from the cells have been successfully suppressed. We can see that there is no damage to the cells

• (FBS, Hyclone) and cultured at 37 °C in a humidified atmosphere with 5% CO2. For cell imaging, cells were treated with F127-CdS/ZnS micelles formulations for 2 h. The cells were then washed with PBS for three times and imaged using a Leica confocal microscopy system (TCS SP2). For the cell viability

Injection of ligand-free gold and silver nanoparticles into murine embryos does not impact pre-implantation development

• Ulrike Taylor,
• Wiebke Garrels,
• Annette Barchanski,
• Svea Peterson,
• Laszlo Sajti,
• Andrea Lucas-Hahn,
• Lisa Gamrad,
• Ulrich Baulain,
• Sabine Klein,
• Wilfried A. Kues,
• Stephan Barcikowski and
• Detlef Rath

Beilstein J. Nanotechnol. 2014, 5, 677–688, doi:10.3762/bjnano.5.80

• remained particle-free. To appreciate the amount of injected nanoparticles, it has to be taken into account that only particles and particle agglomerates with a diameter >60 nm can be visualized by confocal microscopy [55]. The injected dispersion contained such particles only to 2.6 and 2.2% for AuNP and

• , Hamburg, Germany). Laser scanning confocal microscopy (LSCM) For nanoparticle imaging purposes, embryos were transferred onto a glass slide within a droplet of PBS without further fixation and examined immediately. Light microscopical visualization of AuNP was performed as previously described by using an

• nanoparticles, exposure to comparable Ag+-ion concentrations resulted in an immediate arrest of embryo development. In conclusion, the results do not indicate any detrimental effect of colloidal gold or silver nanoparticles on the development of murine embryos. Keywords: biomedical application; confocal

The softening of human bladder cancer cells happens at an early stage of the malignancy process

• Jorge R. Ramos,
• Joanna Pabijan,
• Ricardo Garcia and
• Malgorzata Lekka

Beilstein J. Nanotechnol. 2014, 5, 447–457, doi:10.3762/bjnano.5.52

• confocal microscopy of early and late stages of ovarian cancer progression [22]. Here, we study the correlation between the elastic properties and the expression and organization of the actin cytoskeleton in human bladder cancer cells. We have chosen four cell lines with various histological grades. Those

Near-field effects and energy transfer in hybrid metal-oxide nanostructures

• Ulrich Herr,
• Barat Achinuq,
• Cahit Benel,
• Giorgos Papageorgiou,
• Manuel Goncalves,
• Johannes Boneberg,
• Paul Leiderer,
• Paul Ziemann,
• Peter Marek and
• Horst Hahn

Beilstein J. Nanotechnol. 2013, 4, 306–317, doi:10.3762/bjnano.4.34

• Eu fluorescence can be suppressed by covering the nanoantennas with a 10 nm thick SiOx layer. Keywords: confocal microscopy; energy transfer; field enhancement; light harvesting; luminescence; nano-antennas; nanosphere lithography; nanostructures; plasmonics; simulation; TiO2 nanoparticles

• stage of the CVR machine (see Figure 1). A big advantage of the regular nanoantenna patterns generated in this way is that the positions of the individual antennas can be identified in confocal microscopy, even though the details of the antenna itself may not be resolved. We have used confocal

• fluorescence generated by conversion of the excitation light to photons with 618 nm from the Eu3+ ions. Figure 10a and Figure 10b show two confocal microscopy images of the same area of the sample. Figure 10a was obtained by using the wavelength region of 530–535 nm, which contains mainly elastically or quasi

Plasmonic oligomers in cylindrical vector light beams

• Mario Hentschel,
• Jens Dorfmüller,
• Harald Giessen,
• Sebastian Jäger,
• Andreas M. Kern,
• Kai Braun,
• Dai Zhang and
• Alfred J. Meixner

Beilstein J. Nanotechnol. 2013, 4, 57–65, doi:10.3762/bjnano.4.6

• = 0.998. The detection was performed by confocal microscopy and the collected signal is one-photon photoluminescence. Figure 7 depicts examples of these kinds of measurements on closed and open oligomer ring structures. One observes a strong interaction of the light field with the plasmonic nanostructures

Diamond nanophotonics

• Katja Beha,
• Helmut Fedder,
• Marco Wolfer,
• Merle C. Becker,
• Petr Siyushev,
• Mohammad Jamali,
• Anton Batalov,
• Christopher Hinz,
• Jakob Hees,
• Lutz Kirste,
• Harald Obloh,
• Etienne Gheeraert,
• Boris Naydenov,
• Ingmar Jakobi,
• Florian Dolde,
• Sébastien Pezzagna,
• Daniel Twittchen,
• Matthew Markham,
• Daniel Dregely,
• Harald Giessen,
• Jan Meijer,
• Fedor Jelezko,
• Christoph E. Nebel,
• Rudolf Bratschitsch,
• Alfred Leitenstorfer and
• Jörg Wrachtrup

Beilstein J. Nanotechnol. 2012, 3, 895–908, doi:10.3762/bjnano.3.100

• . Subsequently, diamond nanocrystals are spin coated onto the substrate. By using a dual atomic force microscope (AFM) and confocal microscopy setup, diamond nanocrystals that contain single color centers are then identified by fluorescence microscopy and second-order photon autocorrelation, and their position

• addressed individually afterwards by confocal microscopy [27]. The nanodiamonds acted as seed crystals in the subsequent overgrowth in the MWPECVD plasma process. In this manner, we produced particles with diameters up to 700 nm, as shown in Figure 15a. Because of the harsh plasma environment the silicon

Assessing the plasmonics of gold nano-triangles with higher order laser modes

• Laura E. Hennemann,
• Andreas Kolloch,
• Andreas Kern,
• Josip Mihaljevic,
• Johannes Boneberg,
• Paul Leiderer,
• Alfred J. Meixner and
• Dai Zhang

Beilstein J. Nanotechnol. 2012, 3, 674–683, doi:10.3762/bjnano.3.77

• -triangles in particular [3][4][5][6] has been investigated both directly and indirectly. Likewise, the plasmonic behaviour of metallic nano-triangle arrays, namely Fischer patterns [7] made by colloid lithography, has been studied to some extent. Optical extinction spectroscopy [8], confocal microscopy [9

The oriented and patterned growth of fluorescent metal–organic frameworks onto functionalized surfaces

• Jinliang Zhuang,
• Jasmin Friedel and
• Andreas Terfort

Beilstein J. Nanotechnol. 2012, 3, 570–578, doi:10.3762/bjnano.3.66

• spectrometer. Epifluorescence images were recorded on an Olympus BX51 fluorescence system. Laser scanning confocal microscopy (LSCM) was carried out on a Zeiss LSM 510 META microscope. (a) The crystallographic cell of [Zn2(adc)2(dabco)]. The directionality of the attachment of carboxylate and monodentate Lewis

Characterization of protein adsorption onto FePt nanoparticles using dual-focus fluorescence correlation spectroscopy

• Pauline Maffre,
• Karin Nienhaus,
• Faheem Amin,
• Wolfgang J. Parak and
• G. Ulrich Nienhaus

Beilstein J. Nanotechnol. 2011, 2, 374–383, doi:10.3762/bjnano.2.43

• . 2fFCS setup The 2fFCS setup is based on a time-resolved confocal microscopy system (Microtime 200, PicoQuant, Berlin, Germany). Instead of using a single excitation laser, the light from two identical, orthogonally polarized pulsed 640 nm diode lasers (LDH-P-C-640B, Picoquant, Berlin, Germany) was