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Search for "proliferation" in Full Text gives 156 result(s) in Beilstein Journal of Nanotechnology.

PLGA nanoparticles as a platform for vitamin D-based cancer therapy

  • Maria J. Ramalho,
  • Joana A. Loureiro,
  • Bárbara Gomes,
  • Manuela F. Frasco,
  • Manuel A. N. Coelho and
  • M. Carmo Pereira

Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135

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  • in the S or/and G2/M phases (Figure 6B,C). Additionally, the observed changes on the cell cycle distribution between control A549 cells and A549 cells treated with free calcitriol for 72 h were not significant (p > 0.05). These results are in agreement with the proliferation studies, where the A549
  • pancreatic cell lines, S2-013 and hTERT-HPNE, was reported. The in vitro proliferation assay showed that the encapsulation of calcitriol enhanced its antiproliferative activity. The efficient cell internalization by an endocytosis mechanism of PLGA NPs and their rapid endo-lysosomal escape observed in this
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Published 12 Jun 2015

Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development

  • Ulrike Taylor,
  • Daniela Tiedemann,
  • Christoph Rehbock,
  • Wilfried A. Kues,
  • Stephan Barcikowski and
  • Detlef Rath

Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66

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  • effect was caused by silver ions. However, it supports findings made on spermatogonial stem cells in vitro, which claimed a decrease in cell proliferation after AgNP exposure [40][41]. Observations concerning female reproductive organs are rather rare as most nanoparticle biodistribution studies have
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Published 05 Mar 2015

Self-assembled anchor layers/polysaccharide coatings on titanium surfaces: a study of functionalization and stability

  • Ognen Pop-Georgievski,
  • Dana Kubies,
  • Josef Zemek,
  • Neda Neykova,
  • Roman Demianchuk,
  • Eliška Mázl Chánová,
  • Miroslav Šlouf,
  • Milan Houska and
  • František Rypáček

Beilstein J. Nanotechnol. 2015, 6, 617–631, doi:10.3762/bjnano.6.63

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  • molecules) formed by ionic cross-linking [40]. The proposed architecture is envisaged to enhance adhesion, proliferation, differentiation of osteoblasts, and thus ultimately, to achieve a better integration of the titanium implant into the bone tissue. Conclusion In the present contribution, we demonstrated
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Published 02 Mar 2015

Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells

  • Syeda Arooj,
  • Samina Nazir,
  • Akhtar Nadhman,
  • Nafees Ahmad,
  • Bakhtiar Muhammad,
  • Ishaq Ahmad,
  • Kehkashan Mazhar and
  • Rashda Abbasi

Beilstein J. Nanotechnol. 2015, 6, 570–582, doi:10.3762/bjnano.6.59

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  • ) assay the cytotoxicity in vitro and the differential effect of different Ag contents in the ZnO nanoparticles affecting cell proliferation was analyzed. The photo-oxidation-mediated cytotoxicity of different NPs was investigated by irradiating the samples with daylight or keeping them in the dark. The
  • (565)NP control and Abs(565)blank represent the background optical density and was measured in NPs only and media only samples. HCEC cells were included as a normal control cell line. Mitochondrial function: cell survival and proliferation assay MTT assay was used to investigate mitochondrial function
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Published 26 Feb 2015

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

  • Shanka Walia and
  • Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

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  • biocompatibility of these NPs was investigated by standard MTT cell proliferation assay. Studies suggested that the cell viability was maintained at 83% even after a high dose of 500 µg·mL−1 of the nanocomposites. To check the applicability of these nanocomposites as fluorescence imaging agents, Gastric SGC7901
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Published 24 Feb 2015

Pulmonary surfactant augments cytotoxicity of silica nanoparticles: Studies on an in vitro air–blood barrier model

  • Jennifer Y. Kasper,
  • Lisa Feiden,
  • Maria I. Hermanns,
  • Christoph Bantz,
  • Michael Maskos,
  • Ronald E. Unger and
  • C. James Kirkpatrick

Beilstein J. Nanotechnol. 2015, 6, 517–528, doi:10.3762/bjnano.6.54

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  • ® in the well was 0.04 mg/mL. Cytotoxicity, determination of cell viability: The viability of the cells was determined as described in our previous studies [9][10][11] using the CellTiter 96® AQueous One Solution Cell Proliferation Assay (MTS, Promega, G3582). After nanoparticle incubation, medium was
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Published 20 Feb 2015

Hematopoietic and mesenchymal stem cells: polymeric nanoparticle uptake and lineage differentiation

  • Ivonne Brüstle,
  • Thomas Simmet,
  • Gerd Ulrich Nienhaus,
  • Katharina Landfester and
  • Volker Mailänder

Beilstein J. Nanotechnol. 2015, 6, 383–395, doi:10.3762/bjnano.6.38

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  • nanoparticles. This was very much expected as the proliferation of hHSCs during the growth factor induced differentiation will decrease the amount of nanoparticles per cell division by a factor of 1/2. For the PLLA particles, the remaining amount was significantly higher than the negative control even after 11
  • . The hHSCs showed a great loss in particle payload during all three lineage differentiations. This is likely due to the high proliferation rates during the differentiation process. In other studies, this dilution due to proliferation activity of the cells has clearly been shown [28][29]. In the
  • the high expression of glycophorin A, which indicates that the erythrocyte differentiation is enhanced with PLLA–Fe particles. This can be explained by the fact that iron in this case is an essential compound for erythrocyte differentiation and, therefore, enhances proliferation in this direction
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Published 05 Feb 2015

Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

  • Lisa Landgraf,
  • Ines Müller,
  • Peter Ernst,
  • Miriam Schäfer,
  • Christina Rosman,
  • Isabel Schick,
  • Oskar Köhler,
  • Hartmut Oehring,
  • Vladimir V. Breus,
  • Thomas Basché,
  • Carsten Sönnichsen,
  • Wolfgang Tremel and
  • Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

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  • used a colorimetric (MTS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium, inner salt; Aqueous One Solution Cell Proliferation Assay, Promega, Germany) and a luminescence (ATPLite assay, PerkinElmer, Germany) based cytotoxicity assay. In this context, cells were
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Published 27 Jan 2015

The effect of surface charge on nonspecific uptake and cytotoxicity of CdSe/ZnS core/shell quantum dots

  • Vladimir V. Breus,
  • Anna Pietuch,
  • Marco Tarantola,
  • Thomas Basché and
  • Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 281–292, doi:10.3762/bjnano.6.26

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  • proliferation, which was confirmed by fluorescence microscopy (Supporting Information File 1, Figure S1). We observed a small fraction of abnormally large cells (twice as large as the normal size), and cells with two nuclei after exposure to the 50 nM solutions of QDs (Supporting Information File 1, Figure S1
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Published 26 Jan 2015

Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants

  • Anna Maria Pappa,
  • Varvara Karagkiozaki,
  • Silke Krol,
  • Spyros Kassavetis,
  • Dimitris Konstantinou,
  • Charalampos Pitsalidis,
  • Lazaros Tzounis,
  • Nikos Pliatsikas and
  • Stergios Logothetidis

Beilstein J. Nanotechnol. 2015, 6, 254–262, doi:10.3762/bjnano.6.24

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  • surface hydrophilicity by forming oxygen-containing groups at the surface and thus to improve cell adhesion and proliferation. The conditions of the plasma modification were properly adjusted in order to induce the desirable chemical surface changes while maintaining surface integrity and morphology. The
  • , which is more apparent in the case of the treated samples indicates the growth and proliferation of the cells in their new microenvironment. Cell adhesion and proliferation According to Figure 4c, fibroblasts seemed to be securely attached and spread on the surface, regardless of the surface treatments
  • . Interestingly, cells seemed to be attached and spread more on the plasma-treated scaffold surfaces compared to the untreated surfaces, during the first day. A notable enhancement of the spreading is observed in the following days, with higher cell confluency and proliferation in the treated scaffolds compared
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Published 22 Jan 2015

Mechanical properties of MDCK II cells exposed to gold nanorods

  • Anna Pietuch,
  • Bastian Rouven Brückner,
  • David Schneider,
  • Marco Tarantola,
  • Christina Rosman,
  • Carsten Sönnichsen and
  • Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21

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  • applications is the design of biocompatible NPs that do not impair with cell viability, proliferation, and adhesion. Therefore assessing the cytotoxicity of nanoparticles is pivotal for nanoparticle research in general [11]. In vitro nanocytotoxicity studies are therefore necessary to minimize possible risks
  • migration, proliferation and tissue formation [19][20]. Mechanical behavior of living cells can be monitored spatially resolved in a concentration and time dependent manner using scanning probe techniques. It is possible to investigate local cellular elastic properties under physiological conditions using
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Published 20 Jan 2015

X-ray photoelectron spectroscopy of graphitic carbon nanomaterials doped with heteroatoms

  • Toma Susi,
  • Thomas Pichler and
  • Paola Ayala

Beilstein J. Nanotechnol. 2015, 6, 177–192, doi:10.3762/bjnano.6.17

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  • have received major attention, starting with the discovery of fullerenes in the late 1980s [1][2], followed by the proliferation of carbon nanotube research from the early 1990s [3][4][5], and coming finally to the latest stage when graphene rose into prominence in the mid-2000s [6][7][8]. Due to the
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Published 15 Jan 2015

Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces

  • Miao Yu,
  • Nico Strohmeyer,
  • Jinghe Wang,
  • Daniel J. Müller and
  • Jonne Helenius

Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15

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  • force spectroscopy; Introduction The regulated adhesion of mammalian cells with the extracellular matrix (ECM) and surrounding cells is crucial in biological processes such as cell migration, differentiation, proliferation, and apoptosis. Since impaired cell adhesion causes a wide range of diseases
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Published 14 Jan 2015

Synthesis of boron nitride nanotubes and their applications

  • Saban Kalay,
  • Zehra Yilmaz,
  • Ozlem Sen,
  • Melis Emanet,
  • Emine Kazanc and
  • Mustafa Çulha

Beilstein J. Nanotechnol. 2015, 6, 84–102, doi:10.3762/bjnano.6.9

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  • dispersion in aqueous media for biological applications [12]. The effect of the PEI-coated BNNTs with respect to viability, metabolism and cell proliferation of human neuroblastoma cell line (SH-SY5Y) was investigated. The PEI-coated BNNTs exposed cells analyzed at different time intervals. The viability
  • , metabolic activity and proliferation of the cells were analyzed with MTT and trypan blue assays. The results showed that the PEI-coated BNNTs did not affect the viability of neuroblastoma cells up to 5 µg/mL [12]. Lahiri et al. studied the behavior of osteoblast cells in a scaffold constructed from the BNNT
  • -embedded polylactide-polycaprolactone (PLC–BNNT) in orthopedic implants [75]. Using real-time PCR methods, they studied the RunX2 gene expression profile, which is a transcription factor responsible for enhancing the cell proliferation. The results of the experiments showed that the PLC–BNNTs increased the
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Published 08 Jan 2015

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

  • Christina Rosman,
  • Sebastien Pierrat,
  • Marco Tarantola,
  • David Schneider,
  • Eva Sunnick,
  • Andreas Janshoff and
  • Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

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  • their spreading. Herein, we assess the adhesion as an indicator for viability since the cells detach during apoptosis. Furthermore, an adherent cell, which has increased its spreading area, is considered to show an active proliferation. Figure 2B shows a typical example of an adherent cell at day one
  • , which has detached at day two, leaving behind the cell debris. From the percentage of adherent cells, we rate their proliferation behavior shown by an active increase in spreading area. Figure 2C shows a representative image of a cell that is alive at day one and which has considerably expanded its
  • reduction in adherence by 30% as compared to the untreated control results from the cell growth on the NH2–PEG nanorod-decorated substrate, whereas immobilization of COOH–PEG nanorods does not have an influence. Proliferation The increase in the spreading area of an adherent cell is interpreted as a sign of
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Published 24 Dec 2014

Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices

  • Stephanie Hirn,
  • Nadine Haberl,
  • Kateryna Loza,
  • Matthias Epple,
  • Wolfgang G. Kreyling,
  • Barbara Rothen-Rutishauser,
  • Markus Rehberg and
  • Fritz Krombach

Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253

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  • persisted up to 72 h (Figure 2). Since an increased WST-1 conversion could also indicate cell proliferation, we examined if there was enhanced cell proliferation by performing a proliferation assay (Click-iT® EdU Alexa Fluor® 488 Imaging Kit) in which the incorporation of 5-ethynyl-2′-deoxyuridine, a
  • nucleoside analogue to thymidine, into replicated DNA can be visualized by confocal laser scanning microscopy. As shown in Figure 3, cell proliferation was not significantly altered as shown by this assay. Responses of PCLS after exposure to (nano)particles Cytotoxic response After 4 h of incubation with 20
  • through WST-1 assay. The results of the WST-1 assay suggest that the viability of PCLS was improved 24 h after preparation. As WST-1 conversion is also an indicator for proliferation, we performed an additional proliferation assay that did not show significant differences in proliferation over time. The
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Published 18 Dec 2014

Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions

  • Cornelia Loos,
  • Tatiana Syrovets,
  • Anna Musyanovych,
  • Volker Mailänder,
  • Katharina Landfester,
  • G. Ulrich Nienhaus and
  • Thomas Simmet

Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250

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  • used superparamagnetic iron oxide nanoparticles. Keywords: amino groups; apoptosis; carboxyl groups; cell proliferation; leukemia cell lines; macrophages; mTOR; polystyrene nanoparticles; Review Applications of polystyrene Polystyrene, one of the most extensively used types of plastic [1], is an
  • -localized with lysosomes independent of the cell type (Figure 3, and [41][42][43]). Further analysis demonstrated that PS-COOH did not affect the THP-1 cell proliferation, whereas PS-NH2 particles virtually immediately terminated the cell division [41]. It is also notable, that the cell size decreased after
  • THP-1 after longer incubation time (Figure 4 and [41]). Similarly, other authors did observe no cytotoxicity of PS-COOH in ovarian cancer and endothelial cells [48][49]. PS-NH2 particles not only inhibited the proliferation of THP-1 cells but after three days of induced exposure of phosphatidyl serine
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Published 15 Dec 2014

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

  • Christina Sengstock,
  • Jörg Diendorf,
  • Matthias Epple,
  • Thomas A. Schildhauer and
  • Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

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  • . analyzed the expression of alkaline phosphatase in MSCs in the presence of Ag-NP (up to 1 μg·mL−1) after prolonged cell sulture (35 d). In contrast to the significant inhibition in cell proliferation observed at the highest concentration of Ag-NP, the authors found no influence on the activity of alkaline
  • Technologies) while using 75 cm2 flasks (Falcon, Becton Dickinson GmbH, Heidelberg, Germany). Cells were maintained at 37 °C in a humidified 5% CO2 atmosphere. hMSCs were sub-cultivated every 7–14 d depending on cell proliferation. Adherent cells were washed with phosphate buffered saline solution (PBS, Life
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Published 10 Nov 2014

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

  • Sebastian Ahlberg,
  • Alexandra Antonopulos,
  • Jörg Diendorf,
  • Ralf Dringen,
  • Matthias Epple,
  • Rebekka Flöck,
  • Wolfgang Goedecke,
  • Christina Graf,
  • Nadine Haberl,
  • Jens Helmlinger,
  • Fabian Herzog,
  • Frederike Heuer,
  • Stephanie Hirn,
  • Christian Johannes,
  • Stefanie Kittler,
  • Manfred Köller,
  • Katrin Korn,
  • Wolfgang G. Kreyling,
  • Fritz Krombach,
  • Jürgen Lademann,
  • Kateryna Loza,
  • Eva M. Luther,
  • Marcelina Malissek,
  • Martina C. Meinke,
  • Daniel Nordmeyer,
  • Anne Pailliart,
  • Jörg Raabe,
  • Fiorenza Rancan,
  • Barbara Rothen-Rutishauser,
  • Eckart Rühl,
  • Carsten Schleh,
  • Andreas Seibel,
  • Christina Sengstock,
  • Lennart Treuel,
  • Annika Vogt,
  • Katrin Weber and
  • Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

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  • extracellular silver nanoparticles, the intracellular occurrence of silver agglomerates of silver-pulsed cells had decreased in a process which was clearly not related to cell proliferation under these conditions (Figure 10). Interestingly, the decrease in the number of particles was almost completely inhibited
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Published 03 Nov 2014

Carbon-based smart nanomaterials in biomedicine and neuroengineering

  • Antonina M. Monaco and
  • Michele Giugliano

Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196

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  • primary focus of a study conducted by Heo and colleagues [95], who developed a graphene/PET film to test the effects of a non-contact field stimulation on cell-to-cell coupling. This film was found to be biocompatible and improved cell proliferation and viability compared to those observed in the control
  • proliferation by up-regulating Ki-67 protein expression (Figure 6). To test whether such a scaffold could be used as a neural stimulation electrode, its electrochemical properties were investigated by cyclic voltammetry, the results showing that electrical stimulation via a capacitive charge injection was
  • chamber (8), directly onto hydrogen-terminated diamond (5). Reproduced from [128] with permission. Copyright 2009 Elsevier. Three-dimensional graphene foam scaffolds allow neural stem cells to adhere and improve their proliferation by up-regulating Ki-67 protein expression. Reproduced from [146] with
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Published 23 Oct 2014

In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?

  • Moritz Nazarenus,
  • Qian Zhang,
  • Mahmoud G. Soliman,
  • Pablo del Pino,
  • Beatriz Pelaz,
  • Susana Carregal-Romero,
  • Joanna Rejman,
  • Barbara Rothen-Rutishauser,
  • Martin J. D. Clift,
  • Reinhard Zellner,
  • G. Ulrich Nienhaus,
  • James B. Delehanty,
  • Igor L. Medintz and
  • Wolfgang J. Parak

Beilstein J. Nanotechnol. 2014, 5, 1477–1490, doi:10.3762/bjnano.5.161

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  • vitro labeling, tracking or sensing, there are multiple studies that have shown that over the time course required to perform such studies, the impact on cellular viability/proliferation at appropriate NP concentrations is minimal and is often comparable to or even less impactful than the use of
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Published 09 Sep 2014

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

  • Dickson Joseph,
  • Nisha Tyagi,
  • Christian Geckeler and
  • Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

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  • was measured by using a microplate reader (FL600, Microplate Fluorescence Reader, Bio-Tek Company) at a wavelength of 570 nm. The concentration of the sample causing 50% inhibition of proliferation of the cells (IC50) was determined by plotting the percentage of cell viability versus the sample
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Published 04 Sep 2014

Influence of the PDMS substrate stiffness on the adhesion of Acanthamoeba castellanii

  • Sören B. Gutekunst,
  • Carsten Grabosch,
  • Alexander Kovalev,
  • Stanislav N. Gorb and
  • Christine Selhuber-Unkel

Beilstein J. Nanotechnol. 2014, 5, 1393–1398, doi:10.3762/bjnano.5.152

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  • parameters such as cell proliferation, as the doubling time of acanthamoeba in axenic culture is on the timescale of days [38]. As acanthamoeba can also be grown in suspension [19], their proliferation might not be strongly influenced by the presence of any substrate. In a recent study, we had investigated
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Published 28 Aug 2014

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

  • Fabian Herzog,
  • Kateryna Loza,
  • Sandor Balog,
  • Martin J. D. Clift,
  • Matthias Epple,
  • Peter Gehr,
  • Alke Petri-Fink and
  • Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

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  • proliferation and migration (chemotaxis) both decreased, and the release of cytokines was affected. Increased IL-8 and decreased IL-6 and vascular endothelial growth factor (VEGF) levels were detected at high Ag NP concentrations [65]. These studies however, were obtained with human mesenchymal stem cells
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Published 26 Aug 2014

PEGylated versus non-PEGylated magnetic nanoparticles as camptothecin delivery system

  • Paula M. Castillo,
  • Mario de la Mata,
  • Maria F. Casula,
  • José A. Sánchez-Alcázar and
  • Ana P. Zaderenko

Beilstein J. Nanotechnol. 2014, 5, 1312–1319, doi:10.3762/bjnano.5.144

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  • ; cancer therapy; iron oxide superparamagnetic nanoparticles; polyethylene glycol; Introduction Camptothecin (CPT) is a quinoline based alkaloid, which exhibits a potent cytotoxic activity against a broad spectrum of tumours [1][2][3]. While most antineoplastic agents inhibit cancer cell proliferation by
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Published 19 Aug 2014
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