Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• undifferentiated cells, mesenchymal stem cells were utilized. Cellular uptake of agents was studied by fluorescence microscopy and induction of cell death was visualized by live/dead assay. Dox-conjugated γ-Fe2O3@P(HPMA-MMAA) particles showed enhanced cytotoxicity in drug-sensitive and drug-resistant tumor cells

• Mouse melanoma cells of B16F10/wt line, human T-leukemia cells of Jurkat, K562, HL-60 lines and its drug-resistant HL-60/vinc sub-line (overexpression of P-glycoprotein) were a kind gift of Prof. Walter Berger, Institute of Cancer Research, Vienna Medical University (Austria). Human mesenchymal stem

• streptomycin at 37 °C in humidified atmosphere containing 5% CO2, while hMSCs were cultured in mesenchymal stem cell growth medium (MSCGM BulletKitTM; Lonza, Italy). All other cells were cultured in RPMI medium supplemented with 10% fetal calf serum, streptomycin (50 µg/mL), and penicillin (50 units/mL) at 37

Adhesive contact of rough brushes

• Qiang Li and
• Valentin L. Popov

Beilstein J. Nanotechnol. 2018, 9, 2405–2412, doi:10.3762/bjnano.9.225

• the square, one can see the pillars, whose heights are statistically distributed. The elastic half-space is represented only by its surface. At the location of the highest pillars, one can see the “spikes”, which stem from pillars which are strongly pressed into the elastic half-space. At lower pillar

• heights (see the side of the contact) one can see the “negative spikes” which stem from the not-yet-destroyed adhesive contacts of individual pillars loaded in tension. The scheme of indenting and pull-off stages of an adhesive contact of exponentially distributed pillars. An example of a pillar structure

Nanoscale characterization of the temporary adhesive of the sea urchin Paracentrotus lividus

• Ana S. Viana and
• Romana Santos

Beilstein J. Nanotechnol. 2018, 9, 2277–2286, doi:10.3762/bjnano.9.212

• proximal extensible stem and a distal flattened disc [13]. The later releases an adhesive secretion that remains attached to the substrate as a footprint after detachment [14]. In sea stars, the adhesive is released through individual pores by two types of adhesive secretory cells found together in the

• determined [26] where a peak around 550 nm (after excitation at 488 nm) and emission over 505–620 nm was observed. a) Collection of Paracentrotus lividus footprints on mica. b) Detailed view of a sea urchin tube foot attached to mica, showing the adhesive disc (D) and the stem (S). c) Optical microscopy (10

The role of adatoms in chloride-activated colloidal silver nanoparticles for surface-enhanced Raman scattering enhancement

• Nicolae Leopold,
• Andrei Stefancu,
• Krisztian Herman,
• István Sz. Tódor,
• Stefania D. Iancu,
• Vlad Moisoiu and
• Loredana F. Leopold

Beilstein J. Nanotechnol. 2018, 9, 2236–2247, doi:10.3762/bjnano.9.208

• transmission electron microscope (STEM), equipped with a cold field emission gun, working at an acceleration voltage of 200 kV. The absorption spectra were recorded with a Jasco V-630 UV–vis spectrometer at 1 nm spectral resolution. Raman and SERS spectra were obtained with a Renishaw InVia Raman microscope

Lead-free hybrid perovskites for photovoltaics

• Oleksandr Stroyuk

Beilstein J. Nanotechnol. 2018, 9, 2209–2235, doi:10.3762/bjnano.9.207

Electrospun one-dimensional nanostructures: a new horizon for gas sensing materials

• Muhammad Imran,
• Nunzio Motta and
• Mahnaz Shafiei

Beilstein J. Nanotechnol. 2018, 9, 2128–2170, doi:10.3762/bjnano.9.202

• deposited directly onto a controlled and selective deposition area using a multifield electrospinning setup as shown in Figure 2. The scanning transmission electron microscopy (STEM) image confirms the uniform distribution of MoO3 NPs inside the electrospun fibers. Similarly, CuO NPs are embedded in

• fabrication strategy for synthesis of SnO2 NFs with a branch-on-stem morphology using electrospinning, oxygen plasma etching, sputtering and annealing. Electrospun PVP NFs were first etched with oxygen plasma to make a hierarchical template. Afterwards, a SnO2 film is deposited by sputtering and the PVP

• template is removed by annealing. The morphology of the NFs is dependent on sputtering time, resulting in uniformly distributed branches all over stem. Jun et al. [77] developed polypyrrole (PPy)-coated SnO2 tube-in-tube structures using single-nozzle electrospinning with a phase separation solvent method

The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa

• Birgit Lengerer,
• Marie Bonneel,
• Mathilde Lefevre,
• Elise Hennebert,
• Philippe Leclère,
• Emmanuel Gosselin,
• Peter Ladurner and
• Patrick Flammang

Beilstein J. Nanotechnol. 2018, 9, 2071–2086, doi:10.3762/bjnano.9.196

• flexible stem and an enlarged, flattened disc [11]. At the level of the disc, the epidermis is specialized into a sensory-secretory epithelium, enabling perception and adhesion [11][12]. For several echinoderm species, the adhesive strength was estimated by measuring the tenacity of single tube feet [13

• substrate, many of their tube feet break, leaving their discs and part of the stems attached [17][19][20]. These observations proved that the tenacity of the produced glue can exceed the tensile strength of the stem. The strong and temporary adhesion of echinoderms was proposed to rely on a duo-gland

• reversible attachment achieved through the hundreds of tube feet arranged in double rows on the oral surface of each arm (Figure 1B). Morphology and ultrastructure of the tube feet External morphology. The folded stem and the flattened adhesive disc were clearly distinguishable in SEM images (Figure 1C). For

High-throughput synthesis of modified Fresnel zone plate arrays via ion beam lithography

• Kahraman Keskinbora,
• Umut Tunca Sanli,
• Margarita Baluktsian,
• Corinne Grévent,
• Markus Weigand and
• Gisela Schütz

Beilstein J. Nanotechnol. 2018, 9, 2049–2056, doi:10.3762/bjnano.9.194

• . This can be attributed to the shifting plasmon resonances of the gold nanowires that make up the zones of the FZPs. A scanning transmission electron microscope (STEM) dark-field overview image of the completed array is shown in Figure 4b. The FZPs mostly exhibited high quality with zones free from

• could pass the inspection via electron microscopy. Hence, the robustness of the fabrication method is supported with a 100% yield. One of these FZPs from the marked region in Figure 4d is depicted in Figure 4e, a higher-magnification STEM dark-field image. The FZP showed similar characteristics to the

• applied to the fabrication of a large array of 64 FZPs in an overnight process. Such arrays of FZPs are proposed as expendable, cheap and high-resolution FZPs for FEL experiments, for lithography applications or wavefront sensing and beam diagnostic applications. Experimental Fabrication and SEM/STEM

Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants

• Aikaterini-Rafailia Tsiapla,
• Varvara Karagkiozaki,
• Veroniki Bakola,
• Foteini Pappa,
• Panagiota Gkertsiou,
• Eleni Pavlidou and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189

• this nanoplatform cytocompatible. In future studies, the interaction with musculoskeletal tissues will be examined with other cell lines such as mesenchymal stem cells (MSCs) or bone marrow stromal cells (BMSC) in order to evaluate the tissue specific response to our scaffolds. This cytocompatible

A differential Hall effect measurement method with sub-nanometre resolution for active dopant concentration profiling in ultrathin doped Si_1−xGe_x and Si layers

• Richard Daubriac,
• Emmanuel Scheid,
• Hiba Rizk,
• Richard Monflier,
• Sylvain Joblot,
• Rémi Beneyton,
• Pablo Acosta Alba,
• Sébastien Kerdilès and
• Filadelfo Cristiano

Beilstein J. Nanotechnol. 2018, 9, 1926–1939, doi:10.3762/bjnano.9.184

• the top Si/BOX interfaces in correspondence of the maximum slope of the oxygen signal. The position of the SiO2/Si interfaces determined in this way (1.2 nm and 1.6 nm below the surface for the DSA- and RTA-annealed wafers, respectively) are in perfect agreement with those found by STEM-EDX

Synthesis of rare-earth metal and rare-earth metal-fluoride nanoparticles in ionic liquids and propylene carbonate

• Marvin Siebels,
• Lukas Mai,
• Laura Schmolke,
• Kai Schütte,
• Juri Barthel,
• Junpei Yue,
• Jörg Thomas,
• Bernd M. Smarsly,
• Anjana Devi,
• Roland A. Fischer and
• Christoph Janiak

Beilstein J. Nanotechnol. 2018, 9, 1881–1894, doi:10.3762/bjnano.9.180

• distributions of the nanoparticles were determined by transmission electron microscopy (TEM) and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM) to an average diameter of (11 ± 6) to (38 ± 17) nm for the REF3-NPs from [BMIm][BF4]. The RE-NPs from [BMIm][NTf2] or PC showed

• nanoparticles were determined by transmission electron microscopy (TEM) and high-angle annular dark-field scanning transmission electron microscopy (HAADF-STEM). The crystalline phases and the absence of impurities (oxides) in RE-NPs and REF3-NPs were identified by powder X-ray diffraction (PXRD) and selected

• in [BMIm][BF4] [12][42] or in the synthesis of metal-fluoride nanoparticles from metal acetate (hydrate) in ethylene glycol and an excess of [BMIm][BF4] [47]. The sizes and size distributions of the REF3 nanoparticles were determined by HAADF-STEM (Figure S4a, Supporting Information File 1) and TEM

A visible-light-controlled platform for prolonged drug release based on Ag-doped TiO₂ nanotubes with a hydrophobic layer

• Caihong Liang,
• Jiang Wen and
• Xiaoming Liao

Beilstein J. Nanotechnol. 2018, 9, 1793–1801, doi:10.3762/bjnano.9.170

• essential to human being, previously published work [26] showed that Zn can greatly improve cell functionality by enhancing osteoblast proliferation and mineralization and promoting mesenchymal stem cell osteogenic differentiation. Meanwhile, its anti-inflammatory properties have also drawn wide attention

Uniform cobalt nanoparticles embedded in hexagonal mesoporous nanoplates as a magnetically separable, recyclable adsorbent

• Can Zhao,
• Yuexiao Song,
• Tianyu Xiang,
• Wenxiu Qu,
• Shuo Lou,
• Xiaohong Yin and
• Feng Xin

Beilstein J. Nanotechnol. 2018, 9, 1770–1781, doi:10.3762/bjnano.9.168

• of approximately 100 nm, which is slightly larger than the thickness of pure CoAl LDH reported in the literature [33]. The morphology and structure of the as-prepared NPLs-2.5-800 sample are observed by TEM (Figure 3A–C) and STEM (Figure 3D). As shown in Figure 3A, well-dispersed Co nanoparticles on

• containing magnetic Co nanoparticles (CoAl2O4 phase) and porous carbon layer by partial reduction Co2+ ions in the lattice of LDH with carbon during carbonization process at 800 °C [15][34]. The Z-contrast image in HAADF-STEM image correlates strongly with the atomic mass. Metallic cobalt is a heavier

• element and thus appears as the bright contrast, whereas the mesopores generated by the consumption of surface carbon are observed as darker contrasting areas in Figure 3D. From the HAADF-STEM image in Figure S5A of Supporting Information File 1, and the corresponding elemental line profiles in Figure S5B

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• of the surface of biomaterials. Surface topographical patterns significantly affect cell adhesion, spreading, morphology, proliferation, and differentiation [1][2][3][4][5]. Surfaces with specific micro/nanopatterns have been developed in order to reduce platelet response [6], to regulate stem cell

• either gelatin or collagen substrates. However, grooves with a width of 100 µm width did not influence osteoblast cell orientation [44]. Good cell alignment in collagen or gelatin grooves has also been reported for NIH-3T3 cells [36], vascular smooth muscle cells [46], and dental pulp stem cells [56]. In

• attachment of other cell types to other patterned surface, an improvement in cell attachment, due to patterning, depends on several different conditions. An increased tendency to attach to a patterned surface compared to a planar surface has been seen with human dental pulp-derived stem cells on tantalum

Toward the use of CVD-grown MoS₂ nanosheets as field-emission source

• Geetanjali Deokar,
• Nitul S. Rajput,
• Junjie Li,
• Francis Leonard Deepak,
• Wei Ou-Yang,
• Nicolas Reckinger,
• Carla Bittencourt,
• Jean-Francois Colomer and
• Mustapha Jouiad

Beilstein J. Nanotechnol. 2018, 9, 1686–1694, doi:10.3762/bjnano.9.160

• and densely packed MoS2 NSs grown on SiO2/Si substrates can be seen from the low-magnification scanning TEM (STEM) image (Figure 4a). The STEM image (Figure 4b) demonstrates that the MoS2 NSs growth occurred perpendicular to the substrate. The height of the NSs ranges from 50 to 70 nm. The

• nm Mo film at 850 °C on SiO2/Si substrates: (a) HAADF-STEM image at low magnification with different observed materials layers marked; (b) TEM image at the interface between the MoS2 NSs and the Pt layer; (c) higher magnification of layer A showing edge dislocation (marked with “T”) in the MoS2

• layers; (d) high-magnification HAADF-STEM image at the interface between “A” and “B” from panel (a); (e–g) FFT analysis over the area marked by the dotted squares in panel (d), from top to bottom respectively. Photographs of (a) wet chemically transferred MoS2 sample on the FTO/glass substrate, the FTO

Optical near-field mapping of plasmonic nanostructures prepared by nanosphere lithography

• Gitanjali Kolhatkar,
• Alexandre Merlen,
• Jiawei Zhang,
• Chahinez Dab,
• Gregory Q. Wallace,
• François Lagugné-Labarthet and
• Andreas Ruediger

Beilstein J. Nanotechnol. 2018, 9, 1536–1543, doi:10.3762/bjnano.9.144

• intensity on the map [31]. The same principle is used with scanning transmission electron microscopy (STEM) and electron energy loss spectroscopy (EELS) [32][33][34]. This technique based on inelastic scattering measures the energy losses of the electrons. At the hot spots, the plasmon excitation results in

Electronic conduction during the formation stages of a single-molecule junction

• Atindra Nath Pal,
• Tal Klein,
• Ayelet Vilan and
• Oren Tal

Beilstein J. Nanotechnol. 2018, 9, 1471–1477, doi:10.3762/bjnano.9.138

• conductance contribution of less than 0.5 G0. Note that this contribution is lower than estimated by the step height. Although the difference can stem from another conduction channel via the molecular junction that does not interact with vibration modes, one should bear in mind that both analysis methods

• by two main channels, each with lower conductance contribution than 1 G0. The deviation from the trivial channel distribution can stem from distorted local electronic structure at the single-atom junction due to the presence of the molecular bridge or other nearby adsorbed molecules, such that the

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

• Jaison Jeevanandam,
• Ahmed Barhoum,
• Yen S. Chan,
• Alain Dufresne and
• Michael K. Danquah

Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98

• incidental NMs; additionally, engineered NMs can be purposely designed to exploit novel features that stem from their small size. It is known that metal NPs may be spontaneously generated from synthetic objects, which implies that humans have long been in direct contact with synthetic NMs and that macroscale

• with the extracellular matrix (ECM) within the stem cells includes influential stem cell behavior through sources of passive mechanical force. A wide structural protein spectrum and polysaccharides of different length scales with dominating nanometer-sized collagen fibrils strands of 35–60 nm diameter

Bioinspired self-healing materials: lessons from nature

• Joseph C. Cremaldi and
• Bharat Bhushan

Beilstein J. Nanotechnol. 2018, 9, 907–935, doi:10.3762/bjnano.9.85

• both vertebrates and invertebrates. Flora will be broken down into herbaceous and woody plants. Herbaceous plants die down to the ground each year and regrow (perennials, annuals, and biennials), whereas woody plants refer to trees and plants that maintain a persistent woody stem above ground year

• [20] detail these phases of healing in a soft tissue wound injury, which are shown in Figure 4A [54]. Immediately after an injury to vertebrate soft tissue, platelets gather at the site of the wound to stem the loss of blood while also preventing foreign and potentially harmful material from entering

• component similar to the hard tissue response. In soft tissue, the short-term response involves clotting of the wound to stem blood flow. Over a longer period, the cellular response leads to remodeling of the epithelial (skin) tissue at the wound site. Stem cell response – In the discussion between

Noble metal-modified titania with visible-light activity for the decomposition of microorganisms

• Maya Endo,
• Zhishun Wei,
• Kunlei Wang,
• Baris Karabiyik,
• Kenta Yoshiiri,
• Paulina Rokicka,
• Bunsho Ohtani,
• Agata Markowska-Szczupak and
• Ewa Kowalska

Beilstein J. Nanotechnol. 2018, 9, 829–841, doi:10.3762/bjnano.9.77

• were modified with silver and gold by photodeposition, and characterized by diffuse reflectance spectroscopy (DRS), X-ray powder diffraction (XRD), X-ray photoelectron spectroscopy (XPS) and scanning transmission electron microscopy (STEM). It was found that silver co-existed in zero valent (core) and

• . Collected spores were diluted 10–50 times with 8.5 g/L NaCl aqueous solution, and then counted. STEM images of modified titania samples: (a) Ag/TiO2(ST01), (b) Au/TiO2(ST01), (c) Ag/TiO2(ST41) and (d) Au/TiO2(ST41). XPS spectra for bare (top left) and gold-modified (bottom left) TiO2(ST01) sample, and

Cyclodextrin-assisted synthesis of tailored mesoporous silica nanoparticles

• Fuat Topuz and
• Tamer Uyar

Beilstein J. Nanotechnol. 2018, 9, 693–703, doi:10.3762/bjnano.9.64

• types (i.e., β-CD, HP-γ-CD and HP-β-CD). By varying the concentrations of additives and CD-type, MSNs in various shapes, such as spherical, aggregate, bean-like and faceted were generated (Figure 1). The particles were characterized in terms of morphology by SEM, TEM and STEM, the surface area by BET

• HP groups. These molecules do not aggregate like β-CD, which might affect the particle formation, and eventually lead to the particles in different shapes and pore structures. For detailed analysis of the faceted particles, TEM, STEM and EDX analyses were performed. Figure 3f shows the TEM images of

• ). Figure 5 shows the TEM and STEM images of the particles. Under all conditions, MSNs were obtained where the particle shape transformed from single particles to aggregates depending on the CD content used. At low HP-γ-CD concentrations, mixed particles with spherical and faceted shape were formed. However

Towards 3D crystal orientation reconstruction using automated crystal orientation mapping transmission electron microscopy (ACOM-TEM)

• Aaron Kobler and
• Christian Kübel

Beilstein J. Nanotechnol. 2018, 9, 602–607, doi:10.3762/bjnano.9.56

• . Keywords: ACOM-TEM; 3D reconstruction; in situ testing; quantitative crystallographic analysis; STEM; Findings For the study of nanostructured material with feature sizes <100 nm, transmission electron microscopy (TEM) is the method of choice due to its high spatial resolution [1][2][3][4]. Even though

Fabrication and photoactivity of ionic liquid–TiO₂ structures for efficient visible-light-induced photocatalytic decomposition of organic pollutants in aqueous phase

• Anna Gołąbiewska,
• Marta Paszkiewicz-Gawron,
• Aleksandra Sadzińska,
• Wojciech Lisowski,
• Ewelina Grabowska,
• Adriana Zaleska-Medynska and
• Justyna Łuczak

Beilstein J. Nanotechnol. 2018, 9, 580–590, doi:10.3762/bjnano.9.54

• photoelectron spectroscopy (XPS), diffuse reflectance spectroscopy (DRS), scanning transmission microscopy (STEM) and the Brunauer–Emmett–Teller (BET) surface area method, whereas the photocatalytic activity was evaluated by the degradation of phenol in aqueous solution under visible light irradiation (λ > 420

• 2 h (heating rate 2 °C/min). Various molar ratios of ILs to TBOT were selected as listed in Table 1. Sample characterization The morphology and size distribution of the TiO2 powders were observed using a Jeol SEM microscope operated at 12 kV and Cs-corrected STEM (high angle annular darkfield, HAADF

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• , Vilnius University, Sauletekio Ave. 7, LT-10257 Vilnius, Lithuania, Laser Research Centre, Vilnius University, Sauletekio al. 9, corp. 3, LT-10222 Vilnius, Lithuania 10.3762/bjnano.9.32 Abstract We created a 3D cell co-culture model by combining nanoengineered mesenchymal stem cells (MSCs) with the

• ; mesenchymal stem cells; quantum dots; spheroids; 3D cell culture; Introduction The recent progress in the development of nanoscale agents opens up new perspectives for targeted drug delivery in cancer diagnostics, imaging and therapy. However, once administered into the body, nanoparticles (NPs) are rapidly

• and their immune privileged nature, mesenchymal stem cells (MSCs) can be used as a delivery vehicle for therapeutic and imaging agents, such as drug-conjugated NPs [3][4]. MSCs are adult stem cells that can be isolated from various organs, including brain, liver, kidney, lung, bone marrow, muscle

BN/Ag hybrid nanomaterials with petal-like surfaces as catalysts and antibacterial agents

• Konstantin L. Firestein,
• Denis V. Leybo,
• Alexander E. Steinman,
• Andrey M. Kovalskii,
• Andrei T. Matveev,
• Anton M. Manakhov,
• Irina V. Sukhorukova,
• Pavel V. Slukin,
• Nadezda K. Fursova,
• Sergey G. Ignatov,
• Dmitri V. Golberg and
• Dmitry V. Shtansky

Beilstein J. Nanotechnol. 2018, 9, 250–261, doi:10.3762/bjnano.9.27

• . HADF-STEM and spatially-resolved EDX mapping (Figure 2) demonstrate that the surface of BN NPs is densely populated with Ag NPs. Thorough structural characterization of individual Ag NPs revealed their fine structure. The HRTEM images of individual Ag NPs are depicted in Figure 2c and 2f. The particles

• means of high-resolution transmission electron microscopy (HRTEM), high-angular dark field scanning TEM (HADF-STEM) imaging and energy-dispersive X-ray spectroscopy (EDX) mapping using a JEM-2100 microscope (JEOL) equipped with an energy-dispersive X-ray spectrometer (Oxford Instruments) and a STEM

• ) and UV decomposition of AgNO3 (g) together with the corresponding low-magnification TEM images (b, e, h) and the corresponding high-resolution TEM images (c, f, i). HADF-STEM (a, d) images together with the corresponding spatially-resolved EDX maps (b, e) of BN/Ag HNMs produced via CVD (a, b, c) and