Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• ). Bioorthogonal nanoparticle detection In addition to the previous strategies to improve the MNP core to enhance their relaxivities, surface modification of nanoparticles also improves their biosensing capabilities by amplifying their targeting valency for DMR applications. Bioorthogonal “click” chemistry has

• chemistry has been successfully adapted to magnetic targeting, so as to improve nanoparticle binding efficiency and detection sensitivity. Termed ‘bioorthogonal nanoparticle detection’ (BOND), this technique provides a novel targeting platform in which Tz and TCO act as the coupling agents [53]. In a two

• -applicable and scalable for biomedical use. BOND-2 has already been successfully adapted for molecular profiling of cell samples by DMR [53], and has now established itself as a major targeting method in our laboratory. Table 1 lists a library of cellular makers tested with BOND-2 and DMR. Miniaturized NMR