Entry of nanoparticles into cells and tissues: status and challenges

• Kirsten Sandvig,
• Tore Geir Iversen and
• Tore Skotland

Beilstein J. Nanotechnol. 2024, 15, 1017–1029, doi:10.3762/bjnano.15.83

• Kirsten Sandvig Tore Geir Iversen Tore Skotland Department of Molecular Cell Biology, Institute for Cancer Research, The Norwegian Radium Hospital, Oslo University Hospital, 0379 Oslo, Norway Centre for Cancer Cell Reprogramming, University of Oslo, 0379 Oslo, Norway Department of Biosciences

• scale bar is 100 nm. Figure 2 was reprinted from [29], Current Opinion in Cell Biology, vol. 23, by K. Sandvig; S. Pust; T. Skotland; B. van Deurs, “Clathrin-independent endocytosis: mechanisms and function“, pages 413-420, Copyright (2011), with permission from Elsevier. This content is not subject to

Micro- and nanotechnology in biomedical engineering for cartilage tissue regeneration in osteoarthritis

• Zahra Nabizadeh,
• Mahmoud Nasrollahzadeh,
• Hamed Daemi,
• Mohamadreza Baghaban Eslaminejad,
• Ali Akbar Shabani,
• Mehdi Dadashpour,
• Majid Mirmohammadkhani and
• Davood Nasrabadi

Beilstein J. Nanotechnol. 2022, 13, 363–389, doi:10.3762/bjnano.13.31

• , Semnan University of Medical Sciences, Semnan, Iran Department of Chemistry, Faculty of Science, University of Qom, Qom 37185-359, Iran Department of Cell Engineering, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Department of Stem Cell and

• Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Department of Epidemiology and Biostatistics, Faculty of Medicine, Semnan University of Medical Sciences, Semnan, Iran 10.3762/bjnano.13.31 Abstract Osteoarthritis, which typically

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• . Plasmid CLLCb-pEGFP was kindly provided by Prof. Ernst J. Ungewickell (Department of Cell Biology, Centre of Anatomy, Hannover Medical School, Germany) and vector pcDNA 3.1 (+) was kindly provided by Katarina Luciakova, DSc. (Cancer Research Institute, Biomedical Research Center SAS, Bratislava, Slovakia

The nanomorphology of cell surfaces of adhered osteoblasts

• Christian Voelkner,
• Mirco Wendt,
• Regina Lange,
• Max Ulbrich,
• Martina Gruening,
• Susanne Staehlke,
• Barbara Nebe,
• Ingo Barke and
• Sylvia Speller

Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20

• Rostock, Albert-Einstein-Str. 23, 18059 Rostock, Germany Department of Cell Biology, Rostock University Medical Center, Schillingallee 69, 18057 Rostock, Germany 10.3762/bjnano.12.20 Abstract The functionality of living cells is inherently linked to subunits with dimensions ranging from several

Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy

• Natalie Frese,
• Patrick Schmerer,
• Martin Wortmann,
• Matthias Schürmann,
• Matthias König,
• Michael Westphal,
• Friedemann Weber,
• Holger Sudhoff and
• Armin Gölzhäuser

Beilstein J. Nanotechnol. 2021, 12, 172–179, doi:10.3762/bjnano.12.13

• field of cell biology for imaging various human and animal cells. These include cartilage [2], cancer [3], liver [4], kidney [5] and stem cells [6], as well as fibrin fibers [7]. To visualize viruses and their host organisms, HIM has so far been applied to image T4 phage-infected E. coli bacteria [8

Bio-imaging with the helium-ion microscope: A review

• Matthias Schmidt,
• James M. Byrne and
• Ilari J. Maasilta

Beilstein J. Nanotechnol. 2021, 12, 1–23, doi:10.3762/bjnano.12.1

• mitotic HeLa cells that HIM is ready for ultrastructure research in cell biology. Research on the ultrastructure of cells with the HIM was subsequently continued by Schürmann et al., who presented HIM micrographs of cell-membrane nanodomains in mammalian cells [16]. HIM entered the field of virology in

Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements

• Maria Suciu,
• Corina M. Ionescu,
• Alexandra Ciorita,
• Septimiu C. Tripon,
• Dragos Nica,
• Hani Al-Salami and
• Lucian Barbu-Tudoran

Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94

• biological properties of SPIONs. Various aspects regarding the synthesis of SPIONs and the application to cell biology or medicine have to be considered. Some properties of SPIONs can be controlled through synthesis, such as core type, oxidation state, functionalization, dimension, form, and concentration

Interactions at the cell membrane and pathways of internalization of nano-sized materials for nanomedicine

• Valentina Francia,
• Daphne Montizaan and
• Anna Salvati

Beilstein J. Nanotechnol. 2020, 11, 338–353, doi:10.3762/bjnano.11.25

• endocytosis field, as research progresses. This is an example of the challenges that interdisciplinary fields such as nanomedicine need to face. In this context, a closer connection with the cell biology and endocytosis communities is desirable [113]. Typical experiments to characterize uptake mechanisms are

• developed techniques that are available in cell biology to characterize pathways and that have not yet been used to study the uptake of nano-sized materials may provide novel insights into this difficult question. For instance, so-called OMICS approaches based on large-scale proteomics and full genome

Small protein sequences can induce cellular uptake of complex nanohybrids

• Jan-Philip Merkl,
• Malak Safi,
• Christian Schmidtke,
• Fadi Aldeek,
• Johannes Ostermann,
• Tatiana Domitrovic,
• Sebastian Gärtner,
• John E. Johnson,
• Horst Weller and
• Hedi Mattoussi

Beilstein J. Nanotechnol. 2019, 10, 2477–2482, doi:10.3762/bjnano.10.238

• groups have investigated mechanisms for intracellular-uptake and in vivo biodistribution of various nanomaterials [9][10][11]. Due to the complexity of nanostructured materials combined with the intricacy of cell biology, it has been proven very difficult to develop a good understanding of what controls

pH-Controlled fluorescence switching in water-dispersed polymer brushes grafted to modified boron nitride nanotubes for cellular imaging

• Saban Kalay,
• Yurij Stetsyshyn,
• Volodymyr Donchak,
• Khrystyna Harhay,
• Ostap Lishchynskyi,
• Halyna Ohar,
• Yuriy Panchenko,
• Stanislav Voronov and
• Mustafa Çulha

Beilstein J. Nanotechnol. 2019, 10, 2428–2439, doi:10.3762/bjnano.10.233

• )-functionalized BNNTs Fluorescence microscopy of living cells has become an integral part of modern cell biology. Most often cellular imaging is provided using fluorescent labels, including fluorescent dyes, nanoparticles, nanocomposites or proteins [55]. This label must meet certain criteria, such as

Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

Engineered superparamagnetic iron oxide nanoparticles (SPIONs) for dual-modality imaging of intracranial glioblastoma via EGFRvIII targeting

• Xianping Liu,
• Chengjuan Du,
• Haichun Li,
• Ting Jiang,
• Zimiao Luo,
• Zhiqing Pang,
• Daoying Geng and
• Jun Zhang

Beilstein J. Nanotechnol. 2019, 10, 1860–1872, doi:10.3762/bjnano.10.181

• cytotoxicity assay kit was obtained from Sigma (USA). Puromycin was purchased from Aladdin (Shanghai, China). All other reagents were of analytical grade and used without further purification. Cells and animals The human glioblastoma cell line, U87MG, was purchased from The Institute of Biochemistry and Cell

• Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences (Shanghai, China). The U87MG-EGFRvIII cell line was purchased from the GeneChem (Shanghai, China). U87MG and U87MG-EGFRvIII cells were cultured in DMEM supplemented with 10% FBS and 1% penicillin/streptomycin at 37 °C in a

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• with a sample volume of 750 µL were used. High-resolution imaging was performed using an aberration-corrected FEI Titan transmission electron microscope (TEM) equipped with a Cs-probe corrector (CEOS Company) and operating at 300 kV [53]. Cell biology Human mesenchymal stem cells (hMSC, 5th to 10th

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• Zdenek Plichta Yulia Kozak Rostyslav Panchuk Viktoria Sokolova Matthias Epple Lesya Kobylinska Pavla Jendelova Daniel Horak Institute of Macromolecular Chemistry CAS, Heyrovského nám. 2, 162 06 Prague 6, Czech Republic Department of Regulation of Cell Proliferation and Apoptosis, Institute of Cell

• Biology, NAS of Ukraine, Drahomanov Str. 14/16, Lviv 79005, Ukraine Inorganic Chemistry and Center for Nanointegration, University of Duisburg-Essen, Universitaetsstr. 5-7, D-45117 Essen, Germany Danylo Halytsky Lviv National Medical University, Pekarska Str. 69, Lviv 79000, Ukraine Institute of

The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa

• Birgit Lengerer,
• Marie Bonneel,
• Mathilde Lefevre,
• Elise Hennebert,
• Philippe Leclère,
• Emmanuel Gosselin,
• Peter Ladurner and
• Patrick Flammang

Beilstein J. Nanotechnol. 2018, 9, 2071–2086, doi:10.3762/bjnano.9.196

• Birgit Lengerer Marie Bonneel Mathilde Lefevre Elise Hennebert Philippe Leclere Emmanuel Gosselin Peter Ladurner Patrick Flammang Biology of Marine Organisms and Biomimetics Unit, Research Institute for Biosciences, University of Mons, 23 Place du Parc, 7000 Mons, Belgium Cell Biology Unit

Nano- and microstructured materials for in vitro studies of the physiology of vascular cells

• Alexandra M. Greiner,
• Adria Sales,
• Hao Chen,
• Sarah A. Biela,
• Dieter Kaufmann and
• Ralf Kemkemer

Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155

• of vascular cell responses under well-defined, controllable culture conditions. These advancements are paving the way for a deeper understanding of vascular cell biology and materials–cell interaction. Here, we review previous work focusing on the interaction of vascular smooth muscle cells (SMCs

• . Thus, in vitro studies using modified artificial surfaces to induce biological responses in these cells are an important experimental model in vascular cell biology and biomaterial research (Figure 3). In this review, we provide an overview of materials and important micro- and nanofabrication

• characteristics of cell-study relevant micro/nanostructured surfaces. In the last section of this review, we evaluate and summarize reports about studies of vascular cells interaction with micro/nanostructured surfaces. Review 1 Fabrication of micro- and nanopatterned substrates for cell biology studies The

Viability and proliferation of endothelial cells upon exposure to GaN nanoparticles

• Tudor Braniste,
• Ion Tiginyanu,
• Tibor Horvath,
• Simion Raevschi,
• Serghei Cebotari,
• Marco Lux,
• Axel Haverich and
• Andres Hilfiker

Beilstein J. Nanotechnol. 2016, 7, 1330–1337, doi:10.3762/bjnano.7.124

• controlled tumor retention. The understanding of endothelial cell biology is a prerequisite to fight cancer growth because all tumors are dependent on the vasculature. A detailed study of the interaction of endothelial cells with nanoparticles is necessary for future applications to manipulate endothelial

Experimental and simulation-based investigation of He, Ne and Ar irradiation of polymers for ion microscopy

• Lukasz Rzeznik,
• Yves Fleming,
• Tom Wirtz and
• Patrick Philipp

Beilstein J. Nanotechnol. 2016, 7, 1113–1128, doi:10.3762/bjnano.7.104

• monatomic primary ion beams has already been applied to many problems. Typical examples include the investigation of chromosomes [5], the localization of arsenic [6] and iodine [7] in human hair, precise localisation of elements at sub-cellular level in cell biology [8], study of uptake processes in

Time-dependent growth of crystalline Au⁰-nanoparticles in cyanobacteria as self-reproducing bioreactors: 2. Anabaena cylindrica

• Liz M. Rösken,
• Felix Cappel,
• Susanne Körsten,
• Christian B. Fischer,
• Andreas Schönleber,
• Sander van Smaalen,
• Stefan Geimer,
• Christian Beresko,
• Georg Ankerhold and
• Stefan Wehner

Beilstein J. Nanotechnol. 2016, 7, 312–327, doi:10.3762/bjnano.7.30

• Languages Team, Campus Koblenz, University Koblenz-Landau) for realizing the image processing tool in HASKELL as well as Rita Grotjahn (Cell Biology / Electron Microscopy, University Bayreuth) for technical assistance.

Nanostructured surfaces by supramolecular self-assembly of linear oligosilsesquioxanes with biocompatible side groups

• Maria Nowacka,
• Anna Kowalewska and
• Tomasz Makowski

Beilstein J. Nanotechnol. 2015, 6, 2377–2387, doi:10.3762/bjnano.6.244

• ]. The focus has been more recently shifted towards hydrophilic surfaces due to their antifouling properties [5]. Cell biology applications also require new materials that could mimic the natural biological environment of cells and resemble the natural extracellular matrix (ECM). The surface chemical

Atomic force microscopy as analytical tool to study physico-mechanical properties of intestinal cells

• Christa Schimpel,
• Oliver Werzer,
• Eleonore Fröhlich,
• Gerd Leitinger,
• Markus Absenger-Novak,
• Birgit Teubl,
• Andreas Zimmer and
• Eva Roblegg

Beilstein J. Nanotechnol. 2015, 6, 1457–1466, doi:10.3762/bjnano.6.151

• Graz, Center for Medical Research, BioTechMed-Graz, Austria Research Unit Electron Microscopic Techniques, Institute of Cell Biology, Histology and Embryology, Center for Medical Research, Medical University of Graz, BioTechMed-Graz, Austria Research Center Pharmaceutical Engineering GmbH, Graz

The distribution and degradation of radiolabeled superparamagnetic iron oxide nanoparticles and quantum dots in mice

• Denise Bargheer,
• Artur Giemsa,
• Barbara Freund,
• Markus Heine,
• Christian Waurisch,
• Gordon M. Stachowski,
• Stephen G. Hickey,
• Alexander Eychmüller,
• Jörg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 111–123, doi:10.3762/bjnano.6.11

• Denise Bargheer Artur Giemsa Barbara Freund Markus Heine Christian Waurisch Gordon M. Stachowski Stephen G. Hickey Alexander Eychmuller Jorg Heeren Peter Nielsen Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg

The fate of a designed protein corona on nanoparticles in vitro and in vivo

• Denise Bargheer,
• Julius Nielsen,
• Gabriella Gébel,
• Markus Heine,
• Sunhild C. Salmen,
• Roland Stauber,
• Horst Weller,
• Joerg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5

• Denise Bargheer Julius Nielsen Gabriella Gebel Markus Heine Sunhild C. Salmen Roland Stauber Horst Weller Joerg Heeren Peter Nielsen Department of Biochemistry and Molecular Cell Biology, University Medical Center Hamburg-Eppendorf, Martinistr. 52, 20246 Hamburg, Germany Institute of Physical

Synthesis of radioactively labelled CdSe/CdS/ZnS quantum dots for in vivo experiments

• Gordon M. Stachowski,
• Christoph Bauer,
• Christian Waurisch,
• Denise Bargheer,
• Peter Nielsen,
• Jörg Heeren,
• Stephen G. Hickey and
• Alexander Eychmüller

Beilstein J. Nanotechnol. 2014, 5, 2383–2387, doi:10.3762/bjnano.5.247

• Biochemistry and Molecular Cell Biology, University Medical Centre Hamburg-Eppendorf, 20246 Hamburg, Germany School of Chemistry and Forensic Science, University of Bradford, Richmond Road, Bradford BD7 1DP, United Kingdom 10.3762/bjnano.5.247 Abstract During the last decades of nanoparticles research, many

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• with silver nanoparticles. Keywords: aerosols; biological properties; cell biology; nanoparticles; nanotoxicology; silver; Review Introduction Silver in the form of ions and nanoparticles is extensively used in consumer products and medical devices [1][2][3][4][5][6][7][8][9][10][11]. This is due to