Internal 3D temperature mapping in biological systems using ratiometric light-sheet imaging and lipid-coated upconversion nanothermometers

• Dannareli Barron-Ortiz,
• Enric Pérez-Parets,
• Rubén D. Cadena-Nava,
• Emilio J. Gualda,
• Jacob Licea-Rodríguez,
• Juan Hernández-Cordero,
• Pablo Loza-Álvarez and
• Israel Rocha-Mendoza

Beilstein J. Nanotechnol. 2025, 16, 2306–2316, doi:10.3762/bjnano.16.159

• estimate temperature through ratiometric imaging of two thermally coupled fluorescence bands. This work demonstrates the feasibility of volumetric temperature mapping in internal biological systems using light-sheet fluorescence microscopy and lipid-coated UCNPs as nanothermometry markers. This approach

• ratios [18][19][20][21][22][23][24][25][26][27][28][29] and polarization anisotropy [30][31]. In biological applications involving fluorescent nanothermometry, light–tissue interactions must be carefully considered for an accurate temperature measurement. To address this, a wide range of luminescent

• volumetric temperature sensing through fluorescence-lifetime or UC nanothermometry. However, these approaches remain limited by point-by-point scanning, shallow penetration depth, and narrow fields of view (typically below 100 µm), restricting their applicability to intact organisms. Beyond these

Time-resolved universal temperature measurements using NaYF₄:Er³⁺,Yb³⁺ upconverting nanoparticles in an electrospray jet

• Kristina Shrestha,
• Arwa A. Alaulamie,
• Ali Rafiei Miandashti and
• Hugh H. Richardson

Beilstein J. Nanotechnol. 2018, 9, 2916–2924, doi:10.3762/bjnano.9.270

• understand temperature-dependent reactions such as protein unfolding inside microjet/microdroplets and microfluidic systems. Keywords: electrospray; microjet; nanothermometry; temperature measurement; time-resolved measurement; upconverting nanoparticles; Introduction There is need and interest to non