Liver-targeting iron oxide nanoparticles and their complexes with plant extracts for biocompatibility

• Shushanik A. Kazaryan,
• Seda A. Oganian,
• Gayane S. Vardanyan,
• Anatolie S. Sidorenko and
• Ashkhen A. Hovhannisyan

Beilstein J. Nanotechnol. 2024, 15, 1593–1602, doi:10.3762/bjnano.15.125

• the bloodstream. Assessment of glutathione peroxidase activity The highest activity of glutathione peroxidase (GPx) was detected in the liver homogenates of group V rats, where the enzyme activity was 15052 ± 364 pkat/mg protein. This activity was 1.98 times higher compared to the GPx activity in

• ) according to the attached BioSystems protocols. Determination of glutathione peroxidase activity in liver tissue To determine the activity of liver glutathione peroxidase (GPx) (EC 1.11.1.9), tissue samples weighing 1–2 g were homogenized in 10 mM potassium phosphate buffer (pH 7.2) on ice using a Potter

Dual-functionalized architecture enables stable and tumor cell-specific SiO₂NPs in complex biological fluids

• Iris Renata Sousa Ribeiro,
• Raquel Frenedoso da Silva,
• Romênia Ramos Domingues,
• Adriana Franco Paes Leme and
• Mateus Borba Cardoso

Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100

• dilution rate of 1:500 for 12 h at 4 °C. The membranes were rewashed with TBS-T buffer and incubated with a secondary antibody (Goat anti-Mouse IgG (H + L) HRP, ThermoFisher) at a ratio of 1:20000 for 2 h (room temperature). After a new series of washes with TBS-T, the peroxidase activity was revealed with

New application of bimetallic Ag/Pt nanoplates in a colorimetric biosensor for specific detection of E. coli in water

• Azam Bagheri Pebdeni,
• Mohammad N. AL-Baiati and
• Morteza Hosseini

Beilstein J. Nanotechnol. 2024, 15, 95–103, doi:10.3762/bjnano.15.9

• Department of Pharmaceutical Biomaterials and Medicinal Biomaterials Research Center,Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran 10.3762/bjnano.15.9 Abstract A fast and sensitive aptasensor was developed using nanoplates with peroxidase activity as a novel approach. E. coli

• . This nanobiosensor has the ability to specifically bind to E. coli, increasing the peroxidase activity of the apt-Ag/Pt NPL. Finally, the blue color of the solution in the contaminated water samples was increased in the presence of 3,3′,5,5′-tetramethylbenzidine (TMB) as a substrate and H2O2. The assay

• ; biosensor; detection; E. coli; peroxidase activity; silver/platinum nanoplate; Introduction One of the most dangerous foodborne infections is caused by E. coli. It can induce hemorrhagic colitis, hemolytic uremic syndrome, and thrombocytopenic purpura. E. coli is responsible for more than 2 million acute

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

• Gennady L. Burygin,
• Polina I. Abronina,
• Nikita M. Podvalnyy,
• Sergey A. Staroverov,
• Leonid O. Kononov and
• Lev A. Dykman

Beilstein J. Nanotechnol. 2020, 11, 480–493, doi:10.3762/bjnano.11.39

• well. After 30 min incubation, the wells were washed twice with 100 μL of PBS containing 0.02% Tween 20, and peroxidase activity was estimated by adding to each well 50 μL of a substrate mixture of 0.03% o-phenylenediamine and 0.02% hydrogen peroxide in 0.1 M sodium citrate buffer (pH 4.5). The enzyme

Magnetic nanoparticles for biomedical NMR-based diagnostics

• Huilin Shao,
• Tae-Jong Yoon,
• Monty Liong,
• Ralph Weissleder and
• Hakho Lee

Beilstein J. Nanotechnol. 2010, 1, 142–154, doi:10.3762/bjnano.1.17

• sequences produced by hTERT activity [54]. More recently, myeloperoxidase (MPO) sensors were generated by attaching phenol-containing molecules, such as dopamine or serotonin, to CLIO nanoparticles [41]. In the presence of peroxidase activity, tyroxyl radicals were formed to cross-link the nanoparticles