Internal 3D temperature mapping in biological systems using ratiometric light-sheet imaging and lipid-coated upconversion nanothermometers

• Dannareli Barron-Ortiz,
• Enric Pérez-Parets,
• Rubén D. Cadena-Nava,
• Emilio J. Gualda,
• Jacob Licea-Rodríguez,
• Juan Hernández-Cordero,
• Pablo Loza-Álvarez and
• Israel Rocha-Mendoza

Beilstein J. Nanotechnol. 2025, 16, 2306–2316, doi:10.3762/bjnano.16.159

• estimate temperature through ratiometric imaging of two thermally coupled fluorescence bands. This work demonstrates the feasibility of volumetric temperature mapping in internal biological systems using light-sheet fluorescence microscopy and lipid-coated UCNPs as nanothermometry markers. This approach

• -sheet microscopy; temperature mapping; upconversion fluorescent nanoparticles; Introduction Biological processes involving energy exchange often manifest as temperature fluctuations. Materials sought to measure such changes should exhibit high sensitivity, accuracy, high spatiotemporal resolution, good

• obtained. For instance, Vetrone et al. [36] used UCNP@PEI as nanothermometers for two-dimensional (2D) temperature mapping inside Hella cells. Similarly, Piñol and co-workers [20] used the FIR of Ln3+ (Sm, Eu)-bearing polymeric micelles to achieve real-time 2D temperature maps of breast metastatic

Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

• cell can be directly used as a fluorescence probe and temperature sensor. A review on the use of fluorescent NDs for tracking in living cells is given in [40] and [41]. Later in this review, we discuss cell temperature mapping by ND NV spins. Direct magnetic imaging in cells using NDs has not yet been

• temperature map was then obtained at each location by converting the shift in D into temperature, using dD/dt = −74 kHz/K. This demonstrated the intracellular temperature mapping in primary cortical neurons with a high diffraction-limited spatial resolution. This approach may be of interest for optogenetics