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Beilstein J. Org. Chem. 2024, 20, 1088–1098, doi:10.3762/bjoc.20.96
Graphical Abstract
Figure 1: A) Deamination of cytosine, dC and C as individual nucleosides or as part of a polynucleotide chain...
Scheme 1: i) Boc2O, DMAP, THF, rt, overnight; ii) aq 5 M NaOH, rt, 3 h, 89% yield over two steps; iii) 3, azo...
Scheme 2: i) NaN3, n-Bu4NHSO4, NaHCO3/CHCl3 (1:1), rt, 20 min, 88% yield; ii) a) H2, Pd/C, CH2Cl2, rt, 3 h; b...
Scheme 3: i) H2, 5% Pd/CaCO3/3% Pb, Et3N, CH2Cl2, rt, 1.5 h, 34% and 21% yield for α- and β-anomer of 18, res...
Figure 2: V0 of A3A mimic-catalysed deamination of 5'-dTTTTCAT in the absence (no inhibitor) and presence of ...
Beilstein J. Org. Chem. 2021, 17, 749–761, doi:10.3762/bjoc.17.65
Figure 1: Illustration of H-bonding in a DNA duplex and a parallel triplex. A) Depiction of Watson–Crick base...
Scheme 1: The synthesis of ONs with Ts and N+ modification using the Staudinger reaction during the solid-pha...
Figure 2: Percentage of intact ONs after 120 min. A) N+ONs; B) Ts-ONs. Percentage of intact ONs was determine...
Figure 3: Representative images of mouse NIH 3T3 fibroblasts incubated with either (A–C) no oligo or 20 µM of...
Figure 4: Representative confocal microscopy section showing the FAM vesicles inside the cell. Mouse NIH 3T3 ...
Beilstein J. Org. Chem. 2016, 12, 1348–1360, doi:10.3762/bjoc.12.128
Figure 1: A) Formation of nucleotide triplets in parallel and antiparallel (relatively to polypurine strand) ...
Figure 2: Synthesis of MGB-fluorophore (A) and MGB-TFO (B) conjugates using CuACC. Linker length and composit...
Figure 3: Bifunctional linkers for conjugation of oligonucleotides and polyamides using CuACC.
Figure 4: The target duplex contains a 29 base pair fragment from HIV proviral DNA [35] and a T4 hairpin is conne...
Figure 5: A) Sequence derived from the murine pericentromere repeat fragment with only one target site for th...
Figure 6: Synthesis of azide- and alkyne-modified MGBs.
Figure 7: Structures of fluorescent probes synthesized by "click chemistry".
Figure 8: Titration of the probes F1-NH2-MM14 (12 µM, A, C) and F1-NH2-TO (10 µM, B, D) by the target DNA dup...
Figure 9: Synthesis of modified oligonucleotides containing an alkyne group.
Figure 10: Gel electrophoresis of oligonucleotides modified by alkyne linkers: A – oligonucleotide HIVP (detec...
Figure 11: TINA-TFOs bearing a 3'-alkyne group for antiparallel triplex formation with the target HIV proviral...
Figure 12: Structures of polyamide-TFO conjugates.
Figure 13: Electrophoresis analysis of samples from reaction mixtures after click reactions between alkyne-TFO...
Figure 14: Electrophoresis analysis of reaction mixtures in 20% denaturing polyacrylamide gel after TINA-TFO-M...
Figure 15: Electrophoretic analysis of reaction mixtures in standard 20% denaturing PAGE after DNA-templated s...
Figure 16: Non-denaturing gel electrophoresis analysis of conjugate 28 with fluorescein-labeled target HIV dup...