Diarylethene-modified nucleotides for switching optical properties in DNA

• Sebastian Barrois and
• Hans-Achim Wagenknecht

Beilstein J. Org. Chem. 2012, 8, 905–914, doi:10.3762/bjoc.8.103

• . MS–FAB m/z (%): 883.4 (25) [M + Na]+; HRMS–ESI (m/z): [M + H]+ calcd for C45H41N4O10S2, 861.2264; found, 861.2282. DNA synthesis: Oligonucleotides were prepared with an Expedite 8909 Synthesizer from ABI by using standard phosphoramidite chemistry. Reagents and CPGs were purchased from ABi and Glen

Investigation of the network of preferred interactions in an artificial coiled-coil association using the peptide array technique

• Raheleh Rezaei Araghi,
• Carsten C. Mahrenholz,
• Rudolf Volkmer and
• Beate Koksch

Beilstein J. Org. Chem. 2012, 8, 640–649, doi:10.3762/bjoc.8.71

• protocols by using a SPOT synthesizer (Intavis, Köln, Germany) as described in detail in [15]. The peptides were synthesized on amino-functionalized cellulose membranes (Whatman, Maidstone, Great Britain) of the ester type prepared by modifying cellulose paper with Fmoc-β-alanine as the first spacer residue

• solid-phase synthesis (0.05 mM scale) using a SyroXP-I peptide synthesizer (MultiSyn Tech GmbH, Witten, Germany) and HOBT/TBTU activation. Manual coupling of β- and γ-amino acids was carried out by HOAT/DIC activation. The molar excess of amino acid and coupling reagents was reduced for β- and γ

Synthesis of diverse dihydropyrimidine-related scaffolds by fluorous benzaldehyde-based Biginelli reaction and post-condensation modifications

• Bruno Piqani and
• Wei Zhang

Beilstein J. Org. Chem. 2011, 7, 1294–1298, doi:10.3762/bjoc.7.150

• heated in a Biotage Initiator microwave synthesizer at 120 °C for 20 min. The resulting mixture was purified by F-SPE eluted with 40 mL of 80:20 MeOH/H2O and then 40 mL of acetone. The acetone fraction was concentrated to give 4c (1.3 g) in 90% yield. Typical Suzuki reaction procedure: Synthesis of 5

• Initiator microwave synthesizer at 140 °C for 30 min. The resulting mixture was purified by flash chromatography to give 5a (24 mg) in 67% yield. Typical procedure for cyclocondensation of 4e,f. Synthesis of methyl 3,7-dimethyl-5-(3-(perfluorooctylsulfonyloxy)phenyl)-5H-thiazolo[3,2-a]pyrimidine-6

• -carboxylate (8b) A solution of 3,4-dihydropyrimidinethione 4f (0.76 g, 1 mmol), chloroacetone (185 mg, 1.5 mmol) in 2 mL water was heated in Biotage Initiator microwave synthesizer at 120 °C for 30 min. The resulting mixture was purified by F-SPE eluted with 30 mL of 80:20 MeOH/H2O and then 30 mL of acetone

Synthesis, reactivity and biological activity of 5-alkoxymethyluracil analogues

• Lucie Brulikova and
• Jan Hlavac

Beilstein J. Org. Chem. 2011, 7, 678–698, doi:10.3762/bjoc.7.80

• the 3',5'-hydroxyl groups, the 5'-hydroxyl group was dimethoxytritylated and the 3'-hydroxyl group phosphitylated to afford phosphoramidite 134. The final phosphoramidite 134 was incorporated into oligodeoxynucleotide sequences via solid-phase synthesis by an automated DNA synthesizer. Modified

Synthetic incorporation of Nile Blue into DNA using 2′-deoxyriboside substitutes: Representative comparison of (R)- and (S)-aminopropanediol as an acyclic linker

• Daniel Lachmann,
• Sina Berndl,
• Otto S. Wolfbeis and
• Hans-Achim Wagenknecht

Beilstein J. Org. Chem. 2010, 6, No. 13, doi:10.3762/bjoc.6.13

• ): = 150.6. Preparation of modified oligonucleotides. Oligonucleotides were prepared on an Expedite 8909 synthesizer from Applied Biosystems (ABI) using standard phosphoramidite chemistry. Reagents and controlled pore glass (CPG) (1 µmol) were purchased from Proligo. The synthesis of DNA oligonucleotides

Gold film- catalysed benzannulation by Microwave- Assisted, Continuous Flow Organic Synthesis (MACOS)

• Gjergji Shore,
• Michael Tsimerman and
• Michael G. Organ

Beilstein J. Org. Chem. 2009, 5, No. 35, doi:10.3762/bjoc.5.35

• materials to replace the glass tube with the plan of making the process more sustainable. Experimental Microwave irradiation experiments All MACOS experiments were performed in 1700 μm (ID) borosilicate capillaries, using a single mode Biotage Smith Creator Synthesizer, operating at a frequency of 2.45 GHz

• ., Bruker Biospin Inc., and York University. The authors are grateful to Biotage Inc. for the donation of a Smith Creator Synthesizer™ and to Syrris Inc. for the donation of the components of an AFRICA microreactor flow system to develop this new methodology. We acknowledge Ross Davidson at Surface Science