Interaction of a pyrene derivative with cationic [60]fullerene in phospholipid membranes and its effects on photodynamic actions

• Hayato Takagi,
• Çetin Çelik,
• Ryosuke Fukuda,
• Qi Guo,
• Tomohiro Higashino,
• Hiroshi Imahori,
• Yoko Yamakoshi and
• Tatsuya Murakami

Beilstein J. Org. Chem. 2024, 20, 2732–2738, doi:10.3762/bjoc.20.231

• in Vm was caused by some modification – most likely oxidation – of the plasma membrane by the photoexcited triad molecule. Taken together, for the realization of rapid control of Vm using such C60-based molecules in the membrane, the suppression of ROS generation is an important consideration. In

• generation by C60 and its derivatives [17][18][19]. The most important: upon the addition of PyBA to catC60-lip, the signal intensities of both types of ROS (1O2 and •OH) were decreased (Figure 5a(iii), b(iii), c(iv)). These results indicate that PyBA suppresses ROS generation by catC60-lip in liposome

• same time, unusually fast conversion of O2•– to •OH was also suggested in this system. The results above suggest that catC60-lip generated both types of ROS (1O2 and •OH) via energy transfer and electron transfer mechanisms. The present results are in line with previous studies of photoinduced ROS

Synthesis and characterization of water-soluble C₆₀–peptide conjugates

• Yue Ma,
• Lorenzo Persi and
• Yoko Yamakoshi

Beilstein J. Org. Chem. 2024, 20, 777–786, doi:10.3762/bjoc.20.71

• and nonionic polymer, poly(vinylpyrrolidone) (PVP) [25] and applied these to several in vitro biological assays to report DNA photocleavage [26] and related ROS generation [27][28], antimicrobial photoactivity [29], chondrogenesis-promoting activity [30][31], photocytotoxicity [32][33], and GST enzyme

Menadione: a platform and a target to valuable compounds synthesis

• Acácio S. de Souza,
• Ruan Carlos B. Ribeiro,
• Dora C. S. Costa,
• Fernanda P. Pauli,
• David R. Pinho,
• Matheus G. de Moraes,
• Fernando de C. da Silva,
• Luana da S. M. Forezi and
• Vitor F. Ferreira

Beilstein J. Org. Chem. 2022, 18, 381–419, doi:10.3762/bjoc.18.43

• reactive oxygen species (ROS) generation, resulted from the interactions between nucleophilic biomolecules and 1,4-naphthoquinonic nucleus of menadione and its derivatives. The presence of an α,β-unsaturated diketone in the quinone structures allows them to accept electrons through reduction processes

• ][41]. The quinone–hydroquinone and quinone–semiquinone interconversions, with ROS generation, are responsible for the wide range of biological activities of menadione and its derivatives. An excess of ROS in the intracellular environment can cause harmful effects on structures such as nucleic acids

Photophysical, photostability, and ROS generation properties of new trifluoromethylated quinoline-phenol Schiff bases

• Inaiá O. Rocha,
• Yuri G. Kappenberg,
• Wilian C. Rosa,
• Clarissa P. Frizzo,
• Nilo Zanatta,
• Marcos A. P. Martins,
• Isadora Tisoco,
• Bernardo A. Iglesias and
• Helio G. Bonacorso

Beilstein J. Org. Chem. 2021, 17, 2799–2811, doi:10.3762/bjoc.17.191

• more polar solvents (DMSO; 65–150 nm and MeOH; 65–130 nm) than in CHCl3 (59–85 nm). Compounds 3 presented good stability under white-LED irradiation conditions and moderate ROS generation properties were observed. Keywords: photophysical properties; photostability; quinoline; ROS generation; Schiff

• . Additionally, good photostability and moderate ROS generation may be interesting features for applying these derivatives in photooxidation and photodamage reactions to biomolecules. Experimental General Unless otherwise indicated, all reagents and solvents were used as obtained from commercial suppliers and

α,ß-Didehydrosuberoylanilide hydroxamic acid (DDSAHA) as precursor and possible analogue of the anticancer drug SAHA

• Shital K. Chattopadhyay,
• Subhankar Ghosh,
• Sarita Sarkar and
• Kakali Bhadra

Beilstein J. Org. Chem. 2019, 15, 2524–2533, doi:10.3762/bjoc.15.245

• a minimal effect of cytotoxicity (≈9.1% necrotic as well as apoptotic cells, respectively), by observing the morphology of the cells under a microscope at a concentration of 15 μg/mL. ROS generation study by DCFDA (2′,7′-dichlorodihydrofluorescein diacetate) by FACS (fluorescence-activated cell

• sorting) The results were further complimented with ROS dependent cytotoxicity in the above cell line. ROS generation, induced by various anticancer agents plays a key role in apoptosis [37]. Cells were treated with SAHA, 11b, 11f and 11g at GI25, GI50 and GI75 concentrations for 24 h and analyzed in the

• with cytotoxicity, percentage of apoptotic and necrotic cell death was also calculated. Data are representative of three independent experiments. ROS generation by DCFDA. The quantitative results of bivariate FITC-Annexin V/PI FCM of HeLa cells after treatment with 11b for different concentrations (at

Isoxazole derivatives as new nitric oxide elicitors in plants

• Anca Oancea,
• Emilian Georgescu,
• Florentina Georgescu,
• Alina Nicolescu,
• Elena Iulia Oprita,
• Catalina Tudora,
• Lucian Vladulescu,
• Marius-Constantin Vladulescu,
• Florin Oancea and
• Calin Deleanu

Beilstein J. Org. Chem. 2017, 13, 659–664, doi:10.3762/bjoc.13.65

• inductors (10 μg/mL and 50 μg/mL). Strong fluorescence densities were observed especially at higher concentration (50 μg/mL) of 3,5-disubstituted isoxazoles. Intensities of fluorescence revealed differences between tested compounds. These compounds can be listed in the increasing order of ROS generation

• Supporting Information File 200: Experimental procedures, characterization data, IR, 1H, 13C and 15N NMR data for all new compounds and fluorescence microscopy images of NO and ROS generation for all 3,5-disubstituted isoxazoles-treated Arabidopsis leaves. Acknowledgments This work was supported by the

• alkynes in the presence of catalytic amounts of copper(I) iodide. The effect of 3,5-disubstituted isoxazoles in generation of ROS and NO in plant tissues was investigated by fluorescent microscopy. The obtained data indicate that some of these compounds are chemical elicitors that induce NO and ROS