Possible bi-stable structures of pyrenebutanoic acid-linked protein molecules adsorbed on graphene: theoretical study

• Yasuhiro Oishi,
• Motoharu Kitatani and
• Koichi Kusakabe

Beilstein J. Org. Chem. 2024, 20, 570–577, doi:10.3762/bjoc.20.49

• graphene. We discuss the expected protein sensors when this type of energy surface appears and provide a guideline for improving the sensitivity, especially as an oscillator-type biosensor. Keywords: biosensor; DFT; PASE; protein; surface adsorption; Introduction Consideration of the atomic-scale motion

• to carbon materials [2][3]. The properties and characteristics of these linker molecules are keys not only to the purpose of protein immobilization, but also to the behavior of the entire biosensor system. In oscillator-based biosensors, further adsorption on the sensor, such as viruses using antigen

• vibrational properties of elastic waves, such as phase and amplitude, are known to be highly sensitive to the mass of adsorbed materials on multilayered graphene. Recently, a biosensor that detects target antigens by elastic wave measurement has been developed [11][12], using the method of immobilizing an

Phenanthridine–pyrene conjugates as fluorescent probes for DNA/RNA and an inactive mutant of dipeptidyl peptidase enzyme

• Josipa Matić,
• Tana Tandarić,
• Marijana Radić Stojković,
• Filip Šupljika,
• Zrinka Karačić,
• Ana Tomašić Paić,
• Lucija Horvat,
• Robert Vianello and
• Lidija-Marija Tumir

Beilstein J. Org. Chem. 2023, 19, 550–565, doi:10.3762/bjoc.19.40

• signalize complex formation (interaction with a biomolecule, cyclodextrine, metal cation, etc.) or change of receptor conformation [5][6]. Employment of pyrene as a biosensor is complicated due to its large aromatic surface's hydrophobicity and fluorescence sensitivity to oxygen. Therefore, modifications of

Sustainable manganese catalysis for late-stage C–H functionalization of bioactive structural motifs

• Jongwoo Son

Beilstein J. Org. Chem. 2021, 17, 1733–1751, doi:10.3762/bjoc.17.122

• 31n, regarded as a potentially viable peptide-based biosensor. Manganese-catalyzed inter- and intramolecular C–H alkenylations Manganese(I)-catalyzed C–H alkenylation of 2-phenylpyridines or N-pyridinylindoles with alkynes is characterized by proximity-induced C–H activation through chelation

A smart deoxyribozyme-based fluorescent sensor for in vitro detection of androgen receptor mRNA

• Ekaterina A. Bryushkova,
• Erik R. Gandalipov and
• Julia V. Nuzhina

Beilstein J. Org. Chem. 2020, 16, 1135–1141, doi:10.3762/bjoc.16.100

• different levels, including DNA, RNA, proteins, and small molecule metabolites. Today, along with other methods in clinical diagnosis biosensors are ubiquitously used in the biomedical field. The first prototype of a biosensor was invented by Leland Clark and Champ Lyons in 1962 as an amperometric Clark

• electrode, covered by immobilizing enzyme glucose oxidase, for the detection of glucose [1]. A biosensor means a small molecular device that traditionally consists of a bioreceptor (enzyme, cell, aptamer, oligonucleotide, antibody, and other) for the specific recognition of the target molecule and a

• . Reproducibility and linearity are also very important as well as costs and ease of manufacturing each component of the biosensor. Unlike proteins or antibodies the nucleic acid-based biosensors (NAs) can be easily commercially synthesized, they are smaller, more stable, and can be repeatedly used without losing

Targeted photoswitchable imaging of intracellular glutathione by a photochromic glycosheet sensor

• Xianzhi Chai,
• Hai-Hao Han,
• Yi Zang,
• Jia Li,
• Xiao-Peng He,
• Junji Zhang and
• He Tian

Beilstein J. Org. Chem. 2019, 15, 2380–2389, doi:10.3762/bjoc.15.230

• the photochromic fluorescence reporter into GSH-responsive MnO2 nanosheets, a highly efficient photoswitchable hybrid biosensor is successfully presented with the demanded functionality for precise cell imaging. Results and Discussion Synthesis of dithienylethene fluorescence reporter (Glyco-DTE) The

• photoswitchable imaging of intracellular GSH With the photochromic glycosheet in hand, we then investigated its applications as a biosensor for targeted intracellular GSH imaging. The presence of the β-ᴅ-galactoside residue offers a selective recognition site for ASGPr receptor which is over-expressed in HepG2

An overview of recent advances in duplex DNA recognition by small molecules

• Sayantan Bhaduri,
• Nihar Ranjan and
• Dev P. Arya

Beilstein J. Org. Chem. 2018, 14, 1051–1086, doi:10.3762/bjoc.14.93

• appropriate curvature helps them to bind with specific DNA sequence [129]. A series of arylimidamide analogues were synthesized and their binding affinities towards DNA minor groove was studied by Wilson et al. via fluorescence displacement titration, circular dichroism, DNase I footprinting, biosensor

Discovery of an inhibitor of the production of the Pseudomonas aeruginosa virulence factor pyocyanin in wild-type cells

• Bernardas Morkunas,
• Balint Gal,
• Warren R. J. D. Galloway,
• James T. Hodgkinson,
• Brett M. Ibbeson,
• Yaw Sing Tan,
• Martin Welch and
• David R. Spring

Beilstein J. Org. Chem. 2016, 12, 1428–1433, doi:10.3762/bjoc.12.137

•  2a). We chose to work with wild-type cells as the data obtained would be of more direct relevance to native situations than if biosensor strains were used [9]. The compound was found to be very active by this assay, inhibiting the production of pyocyanin by 86 ± 1% without affecting bacterial growth

• be exploited in a therapeutic context for the development of novel antipseudomonal agents. In this context, the use of wild-type cells in this study is notable, since the data obtained are of more direct relevance to native situations than if biosensor strains (tailored bacterial reporter strains

Learning from the unexpected in life and DNA self-assembly

• Jennifer M. Heemstra

Beilstein J. Org. Chem. 2015, 11, 2713–2720, doi:10.3762/bjoc.11.292

• , addition of the target molecule shifts this equilibrium to favor displacement of the complementary strand, thus generating the dose-dependent signal. Using the previously reported structure-switching biosensor for L-tyrosinamide (L-Tym) [21], we synthesized both the L- and D-DNA sequences, but labeled

Electrochemical oxidation of cholesterol

• Jacek W. Morzycki and
• Andrzej Sobkowiak

Beilstein J. Org. Chem. 2015, 11, 392–402, doi:10.3762/bjoc.11.45

• nanoparticles [65], and zinc oxide nanorods directly grown on silver [66], has also been reported on. The “cholesterol self-powered biosensor” [67], in which the cathodic process is determined by cholesterol oxidase and on an anode phenothiazine-mediated oxidation of cholesterol as well as immobilization of

Synthesis and biological evaluation of novel N-α-haloacylated homoserine lactones as quorum sensing modulators

• Michail Syrpas,
• Ewout Ruysbergh,
• Christian V. Stevens,
• Norbert De Kimpe and
• Sven Mangelinckx

Beilstein J. Org. Chem. 2014, 10, 2539–2549, doi:10.3762/bjoc.10.265

• biosensor (Table 1). Escherichia coli JB523 is a highly sensitive reporter strain that contains plasmid pJBA130 derived from the LuxR-PluxI quorum sensing operon of Vibrio fischeri expressing the production of stable green fluorescent protein (GFP) in response to exogenous AHLs [31]. In addition, the

• ability of this biosensor to respond to a broad range of chain lengths and functionalization makes it a good choice for the study of structure–activity relationships of synthesized analogues. The ability of the natural AHLs 3 and their halogenated analogues 6, 8 and 11 to induce fluorescence was expressed

• range of tested concentrations (1000–10 nM) (Table 1). In correspondence with previous studies this indicates that the 3-oxo moiety of the fatty acid chain is not an absolute requirement for activity in this biosensor [32]. As expected, the activity of the natural AHLs 3 decreased with increasing acyl

Carbohydrate PEGylation, an approach to improve pharmacological potency

• M. Eugenia Giorgi,
• Rosalía Agusti and
• Rosa M. de Lederkremer

Beilstein J. Org. Chem. 2014, 10, 1433–1444, doi:10.3762/bjoc.10.147

• as a biosensor of glucose [40]. A similar strategy was applied to the recombinant human thyroid-stimulating hormone (rhTSH, Thyrogen). Terminal sialic acids were oxidized with sodium periodate to generate aldehydes, which reacted with aminoxi-PEGs (Scheme 4B). The use of this PEGylating agent

Cyanoethylation of the glucans dextran and pullulan: Substitution pattern and formation of nanostructures and entrapment of magnetic nanoparticles

• Kathrin Fiege,
• Heinrich Lünsdorf,
• Sevil Atarijabarzadeh and
• Petra Mischnick

Beilstein J. Org. Chem. 2012, 8, 551–566, doi:10.3762/bjoc.8.63

• years their importance for special biochemical or pharmaceutical applications, such drug delivery or biosensor technology, has grown, due to their special properties such as water solubility, low viscosity and film formation [24][25][26][27]. Pullulan is a homopolysaccharide of D-glucose secreted by

Synthesis of coumarin or ferrocene labeled nucleosides via Staudinger ligation

• Ivana Kosiova,
• Andrea Janicova and
• Pavol Kois

Beilstein J. Org. Chem. 2006, 2, No. 23, doi:10.1186/1860-5397-2-23

• different approaches for modification of nucleic acid components and DNA biosensor construction have been developed, many questions remain to be answered with respect to the complete understanding of optical and electrical properties of modified nucleic acids used in bioanalytical systems. Universal