Wet-spinning of magneto-responsive helical chitosan microfibers

• Dorothea Brüggemann,
• Johanna Michel,
• Naiana Suter,
• Matheus Grande de Aguiar and
• Michael Maas

Beilstein J. Nanotechnol. 2020, 11, 991–999, doi:10.3762/bjnano.11.83

• [23][24]. Since cells are also on the same length scale, these fibers can be used as soft biological actuators to mechanically stimulate cell growth [25]. During wet-spinning, chitosan is extruded through thin needles from acidic solutions into a coagulation bath [24]. Typical coagulants used are

• cell behavior in vivo by applying external stimuli [41][42]. Emerging fields, such as magnetic tissue engineering, which uses magnetic levitation to control cell growth, would greatly benefit from the use of magnetic scaffolds since these would replace the need for treating the cells with magnetic iron

Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

• Gayathri Kandasamy,
• Elena N. Danilovtseva,
• Vadim V. Annenkov and
• Uma Maheswari Krishnan

Beilstein J. Nanotechnol. 2020, 11, 354–369, doi:10.3762/bjnano.11.26

• . Detailed synthesis and characterization of the polymer was earlier reported by Prof Annenkov [21]. The lung cancer cell line A549 was procured from the National Centre for Cell Sciences (NCCS), Pune. Human umbilical vein endothelial cells (HUVECs) were procured from ATCC, USA. EGMTM endothelial cell growth

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

• glutathione concentration (ca. 10 mM), and iii) very recently, tumor hypoxia (i.e., low oxygen concentrations due to rapid use of blood supply for cancer cell growth) [55]. A typical exogenous trigger is light that can induce the cleavage of covalently linked groups and the solubilization and degradation of

Fully amino acid-based hydrogel as potential scaffold for cell culturing and drug delivery

• Dávid Juriga,
• Evelin Sipos,
• Orsolya Hegedűs,
• Gábor Varga,
• Miklós Zrínyi,
• Krisztina S. Nagy and
• Angéla Jedlovszky-Hajdú

Beilstein J. Nanotechnol. 2019, 10, 2579–2593, doi:10.3762/bjnano.10.249

• viability of the growth of PDLCs on the different hydrogels was tested (Figure 6a). After one day of cell growth, the highest cell viability index (referring to the highest cell adhesion) was measured on the 100CYS-LYS gel, which does not contain any LYS at all, only the redox sensitive cross-linker CYS. On

• day one, the cell viability index was lower for the 20CYS-LYS gel and the 40CYS-LYS gel than for the 100CYS-LYS gel. Still, for both gels, it had doubled after three days of cell growth suggesting that a lower CYS concentration favors cell proliferation. This result is presumably related to the

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• vivo cancer imaging. In this report we decorated pegylated liposomes with a GRPR antagonist peptide and studied its interaction with, and accumulation within, lung cancer cells. Results: An N-terminally cysteine modified GRPR antagonist (termed cystabn) was synthesised and shown to inhibit cell growth

• ] and imaging of breast [8], pancreatic [9] and glioma [10] tumours. The use of GRPR antagonists is motivated by their inability to cause downstream cell growth effects, but is counter-balanced by a greatly reduced rate of receptor internalisation. Nonetheless, a number of reports have illustrated that

• ) caused no change in cell growth. These cell proliferation studies were performed in serum-free conditions in line with previous studies [24][25] for two principle reasons. Firstly, the removal of serum from the culture medium depletes bovine bombesin-like peptides that could otherwise stimulate cell

Atomic force acoustic microscopy reveals the influence of substrate stiffness and topography on cell behavior

• Yan Liu,
• Li Li,
• Xing Chen,
• Ying Wang,
• Meng-Nan Liu,
• Jin Yan,
• Liang Cao,
• Lu Wang and
• Zuo-Bin Wang

Beilstein J. Nanotechnol. 2019, 10, 2329–2337, doi:10.3762/bjnano.10.223

• for the tissue regeneration therapy in biomedicine. Keywords: atomic force acoustic microscopy (AFAM); cell growth; nanopattern; stiffness; SU-8 photoresist; topography; Introduction The interactions of cells with extracellular matrices (ECMs) play important roles in regenerative medicine and tissue

• that the nanopattern, although too small to restrict the cell growth, may enhance the elongation of the cells and affect the cellular arrangement. Moreover, the surface roughness of various samples was investigated using the morphological AFAM images (Supporting Information File 1, Figure S2). The

Synthesis and potent cytotoxic activity of a novel diosgenin derivative and its phytosomes against lung cancer cells

• Liang Xu,
• Dekang Xu,
• Ziying Li,
• Yu Gao and
• Haijun Chen

Beilstein J. Nanotechnol. 2019, 10, 1933–1942, doi:10.3762/bjnano.10.189

• calculated. The novel Di derivative FZU-0021-194-P2 (P2) showed the most efficient suppression of cell growth (Figure 1). P2 was characterized by 1H NMR (Supporting Information File 1, Figure S1), 13C NMR (Supporting Information File 1, Figure S2) and HRMS. The structure of P2 was further established by X

Nanoarchitectonics meets cell surface engineering: shape recognition of human cells by halloysite-doped silica cell imprints

• Elvira Rozhina,
• Ilnur Ishmukhametov,
• Svetlana Batasheva,
• Farida Akhatova and
• Rawil Fakhrullin

Beilstein J. Nanotechnol. 2019, 10, 1818–1825, doi:10.3762/bjnano.10.176

• formation of silica/halloysite cell-mimicking imprints. Then the shells were disintegrated using sonication, while the cell debris was dissolved by acid washing to produce polydisperse silica/halloysite cells imprints. Next, these imprints were utilised to selectively recognise HeLa cells in cell growth

• not adhere or proliferate, which was confirmed by optical microscopy (Figure 3C,D). One might expect that the deposition of silica/halloysite shells kills the HeLa cells, which lack any rigid cell protective structure such as cell walls in microbial cells [37], and that therefore no visual cell growth

Materials nanoarchitectonics at two-dimensional liquid interfaces

• Katsuhiko Ariga,
• Michio Matsumoto,
• Taizo Mori and
• Lok Kumar Shrestha

Beilstein J. Nanotechnol. 2019, 10, 1559–1587, doi:10.3762/bjnano.10.153

Biocompatible organic–inorganic hybrid materials based on nucleobases and titanium developed by molecular layer deposition

• Leva Momtazi,
• Henrik H. Sønsteby and
• Ola Nilsen

Beilstein J. Nanotechnol. 2019, 10, 399–411, doi:10.3762/bjnano.10.39

• films were relatively hydrophilic (Ti-thymine (deposited at 225 °C) = 19 ± 2°, Ti-uracil (deposited at 225 °C) = 19 ± 1°, and Ti-adenine (deposited at 250 °C) = 45 ± 3°), Figure 6. This hydrophilicity is highly suitable for cell growth purposes. A similar hydrophilic nature was observed for Ti-glycine

Targeting strategies for improving the efficacy of nanomedicine in oncology

• Gonzalo Villaverde and
• Alejandro Baeza

Beilstein J. Nanotechnol. 2019, 10, 168–181, doi:10.3762/bjnano.10.16

• vectorization for enhancing the cell growth inhibition. In these cases, the goal is to drive the payload to the nucleus after selective internalization in the cytoplasm. Nuclear delivery with the HIV trans-activator of transcription (TAT) peptoid in combination with the vasculature and tumor cell membrane

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• ; the particle size associated with the controlled reaction time and the annealing of the material at 375 °C slightly negatively influenced the interaction with cells, but no toxic effect was observed. Most of the materials induced cell growth at low concentration levels. The overdose effect is best

• seen at 300 µg/mL. For A549 cells, no toxicity was noted in the tested concentration range and there was no strong cell-growth inhibition. BEAS-2B cells were the least affected by the presence of HAp nanoparticles, and no strong growth inhibitions were noted in this case as well. A toxic effect on the

• ][64][65]. Cell-growth inhibition could be related to the presence of a slight excess of calcium ions inside the cytoplasm, which disrupts the intracellular calcium homeostasis and inhibits cells transcriptional and translational processes [66][67], slowing down proliferation and cell growth. Calcium

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• streptomycin at 37 °C in humidified atmosphere containing 5% CO2, while hMSCs were cultured in mesenchymal stem cell growth medium (MSCGM BulletKitTM; Lonza, Italy). All other cells were cultured in RPMI medium supplemented with 10% fetal calf serum, streptomycin (50 µg/mL), and penicillin (50 units/mL) at 37

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• (NIH 3T3). We observed an IC50 value of 4.5 µg/mL and 6.5 µg/mL in A549 and HCT-15 cell lines, respectively (Figure 4B,C). Furthermore, upon administration of 5-FU at a concentration of 100 µg/mL, a 67.02% and 81.5% reduction in cell growth in HCT-15 and A549 cell lines was observed. CaP@5-FU NPs

• population corroborates the previous results of the ROS induction assay, AO/EB observations, and SEM micrographs. The results infer the detrimental effect of 5-FU-induced toxicity on cell growth and multiplication [50]. The change in the mitochondrial membrane potential over the course of a 24 h treatment

Fabrication of photothermally active poly(vinyl alcohol) films with gold nanostars for antibacterial applications

• Mykola Borzenkov,
• Maria Moros,
• Claudia Tortiglione,
• Serena Bertoldi,
• Nicola Contessi,
• Silvia Faré,
• Angelo Taglietti,
• Agnese D’Agostino,
• Piersandro Pallavicini,
• Maddalena Collini and
• Giuseppe Chirico

Beilstein J. Nanotechnol. 2018, 9, 2040–2048, doi:10.3762/bjnano.9.193

• explored in numerous studies to eradicate cancer cells, controlled drug delivery and enhancement of cell growth [1][2][3][4][5]. Among the many types of such nanoparticles, gold nanostars (GNSs) (having a well-tunable LSPR position in the biotransparent NIR window (750–1300 nm) and ease of surface

• irradiation with a 1064 nm laser, as registered by a thermocamera (laser intensity 4 W/cm2). (b) After 5 minutes of laser irradiation (or simply exposure to the air for the control samples) the films were transferred to LB broth and left at 37 °C for cell growth. The number of bacteria per mL was calculated

Synthesis of carbon nanowalls from a single-source metal-organic precursor

• André Giese,
• Sebastian Schipporeit,
• Volker Buck and
• Nicolas Wöhrl

Beilstein J. Nanotechnol. 2018, 9, 1895–1905, doi:10.3762/bjnano.9.181

• superhydrophilic properties, which has a significant effect on cell growth, making CNWs an interesting material for biotechnology (bio-sensors) and medical technology (implants, diagnostics) [13][17]. The growth of CNWs is often referred to as a three-stage process that was originally proposed by Kondo and co

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

• Jaison Jeevanandam,
• Ahmed Barhoum,
• Yen S. Chan,
• Alain Dufresne and
• Michael K. Danquah

Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• show any significant toxicity in either cancerous or non-cancerous cells, implying that the tumour cell growth inhibition properties observed in the spice-derived C-dots can be attributed to the starting material employed for their fabrication. These results evidence that functional groups in the

• significant effect on cell viability neither in LN-229 nor in HK-2 cells (Figure 8) suggests that the inhibition effect on the cell growth of LN-229 cells can be attributed to the nature of the spice-based C-dots, indeed depending on the starting material employed for the C-dots synthesis. Considering the

• reduction in cell viability was observed for LN-229 cells after 24 h of exposure to increasing concentrations of each C-dot type. In fact, results obtained showed that 2 mg/mL of cinnamon, red chilli, turmeric and black pepper C-dots yielded cancer cell growth inhibition efficiencies of 35, 50, 50 and 75

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• [14][15][16]. Moreover, in vitro 3D cultures could fill the gap between 2D in vitro testing and in vivo animal models [17][18]. Spheroid-based 3D models are widely used to test cancer cell growth/proliferation, invasion, angiogenesis, and immune interactions and have been employed in drug screening

Liquid-crystalline nanoarchitectures for tissue engineering

• Baeckkyoung Sung and
• Min-Ho Kim

Beilstein J. Nanotechnol. 2018, 9, 205–215, doi:10.3762/bjnano.9.22

• organizations in living tissues [85]. For instance, Giraud–Guille and colleagues investigated hierarchically ordered surfaces of dense collagen in the cholesteric phase for the use of tissue regeneration templates for aligned cell growth with controlled functions [86]. The collagen fibrils, which are animal

• induced 2D and 3D aligned cell growth in response to the lamellar molecular structure of LCE matrices, with tunable porosity and crosslinking density [99][100][101]. In addition, the 3D anisotropic cell scaffolds can be fabricated by using nematic LCE microspheres as building blocks by tuning elastic

• production of physiologically functional tissue replicas of nerves by controlling 3D cell alignment and growth direction. When cardiomyocytes are embedded in the anisotropic gel scaffolds, the guided cell growth parallel to the major axis of the bundle structure enables the long-range propagation of action

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• ). It is known that progression of CIN from mild dysplasia to invasive cancer is accompanied by the increase in level of epidermal growth factor receptor (EGFR). The overexpression of EGFR has been correlated to uncontrolled cell growth and inhibition of cell apoptosis. Hence, EGFR can be used as a

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• : Human breast cancer cell lines MDA-MB-231 and MCF-7 were obtained from the American Type Culture Collection (ATCC HTB-26™; ATCC HTB-22™). MDA-MB-231 and MCF-7 cells were cultured in cell growth medium (DMEM, Gibco, US), supplemented with 10% (v/v) fetal bovine serum (FBS) (Gibco, US), 100 U/mL

• wells were left without upconverting particles to serve as the control group. After 24 h of treatment, the cell growth medium with nanoparticles was aspirated and cells were washed with DPBS (pH 7.0) three times. To prepare an XTT solution, 0.1 mL activation solution (N-methyl dibenzopyrazine methyl

Laser processing of thin-film multilayer structures: comparison between a 3D thermal model and experimental results

• Babak B. Naghshine and
• Amirkianoosh Kiani

Beilstein J. Nanotechnol. 2017, 8, 1749–1759, doi:10.3762/bjnano.8.176

• can have many potential applications including patterning the cell growth for biomedical applications and controlling the grain size in fabrication of polycrystalline silicon (poly-Si) thin-film transistors (TFTs). Keywords: 3D transient modelling; heat transfer; laser materials processing

• shape of the heat affected zone and ablated area can be significantly altered by applying a thin film on the surface. As mentioned earlier these are the areas that become less and more biocompatible respectively. As a result, controlling the size of these areas makes the concept of patterning the cell

• growth by laser processing even more attractive. Conclusion The model introduced in this paper is able to precisely predict the ablation depth and temperature field of the thin-film coated samples processed by a single nanosecond pulse. Silicon substrates coated with gold and aluminium thin films and

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• Republic 10.3762/bjnano.8.165 Abstract Diamond nanoparticles, known as nanodiamonds (NDs), possess several medically significant properties. Having a tailorable and easily accessible surface gives them great potential for use in sensing and imaging applications and as a component of cell growth scaffolds

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

• Kati Erdmann,
• Jessica Ringel,
• Silke Hampel,
• Manfred P. Wirth and
• Susanne Fuessel

Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132

• approximately a third of the chemotherapeutic concentration when carbon nanomaterials were used in combination (Table 2). Effect on short- and long-term cellular proliferation Next, the influence of the single and combinatory treatments on cell growth was evaluated. Similar to the previous study [27], CNFs and

• expected, DTX (2.5 ng/mL) and MMC (0.5 µg/mL) alone promoted a significant inhibition by about 30% and 75%, respectively, of both short- and long-term proliferation (Figure 2). This inhibitory effect on cell growth was further enhanced when the chemotherapeutics were administered in combination with CNFs

• viability and apoptosis following treatment. An enhanced inhibition of tumor cell growth mediated by DTX–CNT complexes could also be observed in in vivo animal models for various tumor entities [18][22]. These studies suggested that DTX–CNT complexes possess a prolonged half-life and higher accumulation