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Search for "cell lines" in Full Text gives 127 result(s) in Beilstein Journal of Nanotechnology.
Bioselectivity of silk protein-based materials and their bio-inspired applications
Beilstein J. Nanotechnol. 2022, 13, 902–921, doi:10.3762/bjnano.13.81
- –usher polymerization pathway in Gram-negative microorganisms involved in pili protein synthesis [79]. For instance, the inhibitor targets pilus chaperone PapD, thereby reducing the adhesion to cell lines by 90% [80]. These pilicides inhibit curli formation in uropathogenic E. coli by preventing the
Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy
Beilstein J. Nanotechnol. 2022, 13, 560–569, doi:10.3762/bjnano.13.47
- migration capacity, and cells on soft substrates (3 kPa) had the lowest migration capacity (Figure 2c). Cell proliferation assays also revealed that both cell lines had a significantly greater ability to proliferate on stiff substrates, and that the proliferation of prostate cancer cells significantly
- protrusion on the surface of the cell pseudopod. In contrast, the surface of PC-3 cells on soft substrates was smoother. In addition, both cell lines on the stiff substrate were observed to be taller than those on the other two substrates, with cell height values ranging from 1–4 μm. The height of the HPV-PZ
Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects
Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41
- dose determined based on cell viability results of 50% or more is appropriate [22][28]. In previous studies, the cytotoxic effect of EGCG, whose antioxidant effect has been proven many times, has been investigated, especially on cancer cell lines. Although there is no cytotoxicity study on the L929
- human cancer cells without causing toxicity problems in normal cells. In general, it has been confirmed that they are relatively nontoxic in healthy L929 cells but toxic in cancer cell lines [29]. Preparation and characterization of formulations Like many phenolic compounds, EGCG is not sufficiently
Engineered titania nanomaterials in advanced clinical applications
Beilstein J. Nanotechnol. 2022, 13, 201–218, doi:10.3762/bjnano.13.15
- titanium (cpTi). Thereafter, human osteoblast-like cell lines (hFOB) were cultured over annealed Ti, PCL, and PCL/TiO2 (2, 5, and 7 wt % TiO2) scaffolds. SEM images of the cell morphologies are shown in Figure 2. The addition of TiO2 nanoparticles enhances the wettability and surface area, thus favoring
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6
- partial PEI oxidation during the synthesis. Here, we demonstrate in vitro dye-free optical imaging and successful gene transfection with luminescent SPION@bPEI, which was further modified for receptor-mediated delivery of the cargo selectively to cancer cell lines overexpressing the epidermal growth
- delivery of an anionic cargo. Besides, a strong intracellular optical signal supports the optically traceable nature of these nanoparticles. SPION@bPEI nanoparticles were further conjugated with Erbitux (Erb), which is an anti-EGFR antibody for targeting EGFR-overexpressing cancer cell lines. SPION@bPEI
- as well as its targeted delivery to epidermal growth factor receptor (EGFR)-positive cancer cell lines, in vitro. Initially, the dose dependent cytotoxicity of the nanoparticles was determined. Then, using a fluorescence microscope, the ability of these nanoparticles to generate intracellular optical
Alteration of nanomechanical properties of pancreatic cancer cells through anticancer drug treatment revealed by atomic force microscopy
Beilstein J. Nanotechnol. 2021, 12, 1372–1379, doi:10.3762/bjnano.12.101
- . The sharp contrast reduced the details of the cell surface microstructure of HDPE6-C7, AsPC-1, and MIA-PaCa-2 cell lines. The corresponding analyses of cell surface roughness is listed in Supporting Information File 1, Table S1, which shows that the surface roughness of MIA-PaCa-2, HDPE6-C7, and AsPC
- a relatively linear FDC while the other three cancer cell lines form nonlinear FDCs, which can be evaluated regarding the slope of the FDCs [25]. The Young’s modulus of the four kinds of cells obtained by fitting the linear part of the withdrawal curves are 14.93, 2.1, 6.24 and 3.74 kPa, which
- ]. The statistical comparison of the energy dissipation in the four types of cell lines is shown in Figure 4b. The energy dissipation in the normal cell line (HDPE6-C7) is smaller than that in the PCCs (AsPC-1, MIA PaCa-2, and BxPC-3) and the stiffer the cells, the larger the energy dissipation. This
Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99
- vitro compared to free drug controls. The IC50 values ranged from 0.81 to 3.97 μg/mL for HepG2 and HT144 cells, whereas IC50 values for normal lymphocytes were 10 to 35 times higher (18.35–43.04 µg/mL). Cobalt ferrite (CFO) and zinc ferrite (ZFO) NPs were highly genotoxic (p < 0.05) in cancer cell lines
- spheroid diameter and up to 74 ± 8.9% of cell death after two weeks. In addition, they also inhibited multidrug resistance (MDR) pump activity in both cell lines suggesting effectivity in MDR cancers. Among the tested MFe2O4 NPs, CFO nanocarriers were the most favorable for targeted cancer therapy due to
- of cell viability were plotted for all doses (Figure 4b) and IC50 values were determined (Table 8). In both cell lines, drug-functionalized NPs caused almost 45–50% reduction in cellular viability at a concentration of 1 µg/mL, and the cellular viability decreased even further at higher doses. In
Use of nanosystems to improve the anticancer effects of curcumin
Beilstein J. Nanotechnol. 2021, 12, 1047–1062, doi:10.3762/bjnano.12.78
- stored at 4 °C) has shown inhibitory effects on A549 (IC50 3.9 µg/mL) and on H460 lung cancer (IC50 2.9 µg/mL) cell lines, according to a cell cycle arrest at G2/M [59]. Morevore, the activities of caspase-3, caspase-8, and caspase-9 had a dose-dependent increase in both cell lines in response to both
- , which yielded a dual targeting structure that was able to cross the blood–brain barrier, a significant obstacle when treating brain tumors. Similarly, an improved internalization of CUR-loaded MNP was also observed in HPAF-II and Panc-1 human pancreatic cancer cell lines (54.06% and 53.86%, respectively
- greater potential to produce ROS [143]. A similar behavior was observed in cervical carcinoma cell lines treated with nanoemulsion-photostimulated CUR, where cell viability was reduced to <5% and an increase in caspase-3 and caspase-7 activity was apparent [144]. Inostroza et al. [112] reported the
The role of deep eutectic solvents and carrageenan in synthesizing biocompatible anisotropic metal nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 924–938, doi:10.3762/bjnano.12.69
- cell lines [32]. Carrageenans are sulfated oligosaccharides extracted from red algae. They are composed of galactose and anhydrogalactose sub-units linked through a glycosidic bond. They are mainly categorized into three types depending on the degree of sulfation. The three types are: kappa carrageenan
- osteoblast cytotoxicity tests in cell lines (L02 and L929). Adapted from [116], J. I. González Ocampo et al., “Evaluation of cytotoxicity and antimicrobial activity of an injectable bone substitute of carrageenan and nano hydroxyapatite”, J. Biomed. Mater. Res. A., with permission from John Wiley and Sons
Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension
Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62
- properties. Several studies have used different types of cell lines under in vitro conditions to investigate the properties, differential responses, and mechanisms of action of Ag-NPs [10]. Sengstock et al. showed that Ag-NPs attenuate the adipogenic and osteogenic differentiation of human MSCs. These
- technology indicate that telomerase maintains cells in a proliferative state, and its downregulation parallels cellular differentiation [14]. Telomerase is an enzyme responsible for maintaining the telomeric repetition at the end of the chromosomes. Different cell lines have different telomere lengths (TL
A review on nanostructured silver as a basic ingredient in medicine: physicochemical parameters and characterization
Beilstein J. Nanotechnol. 2021, 12, 440–461, doi:10.3762/bjnano.12.36
- ability of AgNPs against infection caused by the respiratory syncytial virus (RSV). Morris et al., by using in vitro and in vivo models in which mice were infected experimentally, concluded that AgNPs effectively reduced RSV replication and pro-inflammatory cytokine production in epithelial cell lines and
The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors
Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31
- -cell-derived factor 1 in discrete, discontinuous areas that influence the homing ability of a variety of tumor cell lines inside the BM, and that these specific molecules determine a microenvironment for early BM tumor metastasis [34]. This specific feature was employed by Mann and co-workers, who
- authors noticed that the cytotoxicity of folate-functionalized liposomes was greater in FR positive cell lines and that the effect could be blocked through the addition of 1 mM folic acid. Additionally, the authors revealed that the functionalized carriers increased the median survival of the mouse
- reflected on the evaluated IC50 values of IM on different cell lines. Additionally, the comparative analysis of the IC50 values on different cell lines revealed that the transferrin receptor expression and the cellular levels of BCR-ABL mRNA affected the efficacy of the formulation. The cell lines with
Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells
Beilstein J. Nanotechnol. 2021, 12, 282–294, doi:10.3762/bjnano.12.23
- cytotoxicity of AgNPs using different cell lines and rodent models [7][21][22][23]. However, some of these cell lines are resistant to toxicity effects that may be induced by AgNPs [24]. Furthermore, there are only a few studies on the bio-uptake and exposure of AgNPs in human PBMCs [25][26][27][28][29][30][31
Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization
Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22
- , albumin-binding proteins are present on various human tumor cell lines derived from solid tumors [59]. In contrast to BSA-SO-MNPs, CavME was the predominant mechanism involved in the uptake of PEG-SO-MNPs. The internalized quantity of PEG-SO-MNPs was substantially lower compared with control cells when F
The nanomorphology of cell surfaces of adhered osteoblasts
Beilstein J. Nanotechnol. 2021, 12, 242–256, doi:10.3762/bjnano.12.20
- the only method capable of nanoscopic three-dimensional imaging of living cells without the application of dye labels or other modifications. Though SICM was developed already in 1989 [19], it was not much exploited until the method was used to image a number of murine and human cell lines [23
Imaging of SARS-CoV-2 infected Vero E6 cells by helium ion microscopy
Beilstein J. Nanotechnol. 2021, 12, 172–179, doi:10.3762/bjnano.12.13
- , while many more cell lines have been reported to be refractory to SARS-CoV-2 infection [15]. Both scanning electron microscopy (SEM) and transmission electron microscopy (TEM) have been used to image SARS-CoV-2 [16][17][18][19][20]. While TEM achieves unsurpassed resolution and can visualize
A review on the green and sustainable synthesis of silver nanoparticles and one-dimensional silver nanostructures
Beilstein J. Nanotechnol. 2021, 12, 102–136, doi:10.3762/bjnano.12.9
Transient coating of γ-Fe2O3 nanoparticles with glutamate for its delivery to and removal from brain nerve terminals
Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122
- extracellular glutamate concentrations in glioma cell lines in vitro was shown to be up to 500 µM, and glutamate stimulates glioma cell proliferation in vivo. Also, glial tumor cells ex vivo generate neurotoxic quantities of glutamate [3][4][5][6]. Excessive extracellular glutamate concentrations of 100 μM were
Straightforward synthesis of gold nanoparticles by adding water to an engineered small dendrimer
Beilstein J. Nanotechnol. 2020, 11, 1110–1118, doi:10.3762/bjnano.11.95
- here at room temperature. Even though a better control of the polydispersity is desirable, other gold nanoparticles of relatively high dispersity have been recently used for drug delivery in three different cell lines [68]. Conclusion In conclusion, we have shown for the first time that a single
Applications of superparamagnetic iron oxide nanoparticles in drug and therapeutic delivery, and biotechnological advancements
Beilstein J. Nanotechnol. 2020, 11, 1092–1109, doi:10.3762/bjnano.11.94
- release at the targeted site. A functionalization that permits only adsorption can lead to premature drug release [33][46][80]. Mojica Pisciotti and co-workers [39] studied the effects of dextran and PEG coatings on two animal kidney cell lines and showed that dextran-coated SPIONs are not cytotoxic even
- worked on three cell types, i.e., heart, kidney, and brain cell lines, with bare SPIONs and SPIONs with negative or positive surface charge. They showed that positively charged nanoparticles are highly toxic and that each cell type responds differently to every type of nanoparticles, genetically
Key for crossing the BBB with nanoparticles: the rational design
Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72
- amplify phages displaying peptides able to interact with the proteins or cell lines of interest [121]. The results of this technique are entirely dependent on the library of DNA sequences used and attention should be given to select tissue-specific peptides [122]. Among others, two interesting peptides
Examination of the relationship between viscoelastic properties and the invasion of ovarian cancer cells by atomic force microscopy
Beilstein J. Nanotechnol. 2020, 11, 568–582, doi:10.3762/bjnano.11.45
- SKOV-3. Therefore, the three cell lines OVCAR-3, HO-8910 and HOSEpiC were selected for the present work [34]. The viscoelastic properties of cells include elasticity and viscosity, which are prominent biomechanical properties of cells. This study, the cell viscoelasticity was derived from the force
- indentation curves obtained with AFM on three different ovarian cell lines shown in Figure 1. The results of cell elasticity showed a concentrated and narrow distribution in OVCAR-3 and HO-8910 cells (Figure 1a-ii,iii) and a dispersive and broad distribution in HOSEpiC cells (Figure 1a-i). The average values
- , migration and invasion [61]. Lian et al. have shown that a drug can inhibit cellular invasion through affecting biomechanical properties of cancer cells [62]. Ech affected the invasion activity of the cell lines in this report, and could present a new treatment regimen for malignant tumors [63]. For
Identification of physicochemical properties that modulate nanoparticle aggregation in blood
Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44
- platelets [16]. Additionally, SNPs were found to induce pre-thrombotic states through surface-driven activation of the coagulation factor XII [17][18]. Finally, SNPs are known to induce oxidative stress in several cell lines including endothelial cells [19] and leucocytes [20][21], a process that in vivo
Luminescent gold nanoclusters for bioimaging applications
Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42
- been previously summarized in several reports [54][78][79][80][81][82]. This review discusses an overview of the application of gold NCs in biosensing and bioimaging. Importantly, the sensing of pathogenic bacteria and viruses, in vitro imaging of cell lines and in vivo bioimaging using animal models
- rapid detection and the development of new immunoassays. Imaging and labeling mammalian cell lines Beyond their antibacterial effect and pathogen sensing, the surface functionalities of NCs allow for selective labeling for the detection of biomolecules, intracellular metal ion sensing, live-cell imaging
- ). The Au-MUDA-NLS NCs were easily internalized and distributed in the nucleus when studied using HeLa cell lines (Figure 4A). The intracellular and nuclear distribution was studied using a membrane dye WGA-Alexa 594 and a nuclear dye, SYTO59. This suggested that Au-MUDA-NLS NCs were well distributed in
Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery
Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41
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