Patterning of supported gold monolayers via chemical lift-off lithography

• Liane S. Slaughter,
• Kevin M. Cheung,
• Sami Kaappa,
• Huan H. Cao,
• Qing Yang,
• Thomas D. Young,
• Andrew C. Serino,
• Sami Malola,
• Jana M. Olson,
• Stephan Link,
• Hannu Häkkinen,
• Anne M. Andrews and
• Paul S. Weiss

Beilstein J. Nanotechnol. 2017, 8, 2648–2661, doi:10.3762/bjnano.8.265

• DNA, or self-assembly and hybridization of DNA on unpatterned PDMS (Figure S5, Supporting Information File 1). Using CLL and fluorescence imaging, we produced images over square-millimeter areas with a lateral feature size spanning several orders of magnitude on the same substrates (Figure 2C,D). We

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

• Joseph R. Pyle and
• Jixin Chen

Beilstein J. Nanotechnol. 2017, 8, 2296–2306, doi:10.3762/bjnano.8.229

• theoretically predicted with the proposed method in this report. Keywords: diffusion; PAINT; single-molecule photophysics; super-resolution imaging; Introduction Fluorescence imaging of DNA with intercalating dyes is important for DNA sensing [1][2], nucleic acid imaging inside cells and viruses [3][4][5

• , height). The height is defined by the thickness of the tape. Fluorescence imaging All fluorescence measurements were carried out with a home-built microscope under total internal reflection fluorescence (TIRF) mode (Figure 2) equipped with four solid state lasers (Dragon Lasers, China), two beam

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• fluorescent CNOs is fast and reversible both in solution and in vitro, making this nanomaterial suitable as pH-dependent probes for diagnostic applications. Keywords: carbon nanomaterials; fluorescence; imaging; nanomedicine; nano-onion; Introduction Nanomaterial-based probes (nano-probes) that are able to

• %) and PBS and incubated with a solution of Hoechst 33342 (5 μg mL−1) (Sigma) for 20 min. Finally, the cells were rinsed three times and filled with PBS. Confocal fluorescence imaging was then carried out with a Nikon A1R laser scanning microscope and a plan apo 20× DIC M and a plan apo VC 60× oil DIC N2

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• fluorescence imaging An intrinsic problem of fluorescence spectroscopy is linked to the fact that both intensity and contrast of autofluorescence in tissue are quite low. Often the spectral difference between normal and pathological tissue can be enhanced by external administration of fluorophores or

• imaging of the specific biomarker of cervical cancer. The bioconjugates of gold nanoparticles (approximately 12 nm in diameter) with antibodies against EGFR have been used to increase the contrast during in vitro confocal reflectance and confocal fluorescence imaging of normal and abnormal cervical cells

Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• fluorescence imaging. Cell barrier growth might be affected by different growth support material [49], but culturing the Caco-2 barriers on a hard surface (coverslides) instead of a porous transwell, allows correlating the imaging results to those coming from flow cytometry (where the barriers were cultured in

• association with cells. Fluorescence imaging Glass coverslips were sterilised in 70% ethanol and placed into a 12-well plate. Caco-2 cells were seeded and grown for 4 and 21 days, as described above. After exposure to nanoparticles, the nanoparticle dispersion was removed and cells rinsed with PBS three times

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• examined via fluorescence imaging using endocytosis inhibitors for the micropinocytosis, phagocytosis, lipid-raft, clathrin- and caveolin-dependent endocytosis pathways. These data showed that QDs were efficiently accumulated in the cytoplasm of MSCs after incubation for 6 h. The main uptake route of QDs

Multiwalled carbon nanotube hybrids as MRI contrast agents

• Nikodem Kuźnik and
• Mateusz M. Tomczyk

Beilstein J. Nanotechnol. 2016, 7, 1086–1103, doi:10.3762/bjnano.7.102

• techniques (fluorescence imaging, PET and others) is an attractive approach to administering one formulation for different tomography and therapeutic purposes. These issues, achieved by (non-)covalent modifications of pristine multiwalled carbon nanotubes (MWCNTs) and oxidized nanotubes are described in this

Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy

• Shanka Walia and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57

• sites in vivo. In this sense, bimodal imaging probes that simultaneously enable magnetic resonance imaging and fluorescence imaging have gained tremendous attention because disease sites can be characterized quick and precisely through synergistic multimodal imaging. But such hybrid nanocomposite

• will cover a full description of MRI-active and fluorescent multifunctional silica micro/nanospheres including the design of the probe, different characterization methods and their application in imaging and treatment in cancer. Keywords: bimodal imaging; fluorescence imaging; magnetic nanoparticles

• (negative contrast, modified T2). Similarly the fluorescent CAs includes different lanthanide complexes, rare earth oxides, organic dyes, QDs, and ruthenium complexes. In this review article, we summarize recent literature reports on multifunctional nanocomposites for MRI and fluorescence imaging that are

Carbon nano-onions (multi-layer fullerenes): chemistry and applications

• Juergen Bartelmess and
• Silvia Giordani

Beilstein J. Nanotechnol. 2014, 5, 1980–1998, doi:10.3762/bjnano.5.207

• fluorescent-tagged CNOs (Scheme 8) were then used for in vitro fluorescence imaging, which will be discussed in the corresponding section of this review article. Recently, Echegoyen et al. reported for the first time the alkylation of CNOs [42], which was achieved by a reductive process utilizing a Na–K alloy

Model systems for studying cell adhesion and biomimetic actin networks

• Dorothea Brüggemann,
• Johannes P. Frohnmayer and
• Joachim P. Spatz

Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131

• later employed to study its interaction with actin filaments. Using fluorescence imaging it could be visualised for the first time that reconstituted talin is able to anchor actin at lipid membranes. Talin was also observed to nucleate actin filaments and to promote growth, as it was reflected by an

• molar affinity of talin to lipid vesicles [77]. The insertion behaviour of talin into negatively charged lipid bilayers was investigated in more detail by the film balance method combined with fluorescence imaging. With this technique, Sackmann and co-workers showed that fluorescently labelled as well

Apertureless scanning near-field optical microscopy of sparsely labeled tobacco mosaic viruses and the intermediate filament desmin

• Alexander Harder,
• Mareike Dieding,
• Volker Walhorn,
• Sven Degenhard,
• Andreas Brodehl,
• Christina Wege,
• Hendrik Milting and
• Dario Anselmetti

Beilstein J. Nanotechnol. 2013, 4, 510–516, doi:10.3762/bjnano.4.60

• University Bochum, Georgstraße 11, D-32545 Bad Oeynhausen, Germany Libin Cardiovascular Institute of Alberta, Department of Cardiac Sciences, University of Calgary, 3280 Hospital Drive NW, T2N4Z6, AB, Canada 10.3762/bjnano.4.60 Abstract Both fluorescence imaging and atomic force microscopy (AFM) are highly

• topography and fluorescence imaging. Commonly, among the various (apertureless) SNOM approaches metallic or metallized probes are used. Here, we report on our custom-built aSNOM setup, which uses commercially available monolithic silicon AFM cantilevers. The field enhancement confined to the tip apex

Near-field effects and energy transfer in hybrid metal-oxide nanostructures

• Ulrich Herr,
• Barat Achinuq,
• Cahit Benel,
• Giorgos Papageorgiou,
• Manuel Goncalves,
• Johannes Boneberg,
• Paul Leiderer,
• Paul Ziemann,
• Peter Marek and
• Horst Hahn

Beilstein J. Nanotechnol. 2013, 4, 306–317, doi:10.3762/bjnano.4.34

• image of the spin-coated TiO2:Eu particles. The surface is characterized by regions of relatively homogeneous particle deposition, but also by the presence of large particles. Good results by confocal fluorescence imaging can only be expected from the smooth regions. Figure 16 shows an AFM image of a

Combining nanoscale manipulation with macroscale relocation of single quantum dots

• Francesca Paola Quacquarelli,
• Richard A. J. Woolley,
• Martin Humphry,
• Jasbiner Chauhan,
• Philip J. Moriarty and
• Ashley Cadby

Beilstein J. Nanotechnol. 2012, 3, 324–328, doi:10.3762/bjnano.3.36

• (SNOM) [1][2][3] and techniques based on adaptations of single-molecule spectroscopy [4], such as fluorescence imaging with one-nanometer accuracy (FIONA) [5], stochastic optical reconstruction microscopy (STORM) [6]. These techniques require the fluorophore under observation to be isolated by distances