Design of a nanostructured mucoadhesive system containing curcumin for buccal application: from physicochemical to biological aspects

• Sabrina Barbosa de Souza Ferreira,
• Gustavo Braga,
• Évelin Lemos Oliveira,
• Jéssica Bassi da Silva,
• Hélen Cássia Rosseto,
• Lidiane Vizioli de Castro Hoshino,
• Mauro Luciano Baesso,
• Wilker Caetano,
• Craig Murdoch,
• Helen Elizabeth Colley and
• Marcos Luciano Bruschi

Beilstein J. Nanotechnol. 2019, 10, 2304–2328, doi:10.3762/bjnano.10.222

• . Consequently, it was evaluated only for the CUR systems. The retention of the systems without CUR have already been evaluated by a similar method, where the formulations were marked with FITC-dextran and the retention was investigated by fluorescence microscopy [70]. The cumulative formulation percentage

BergaCare SmartLipids: commercial lipophilic active concentrates for improved performance of dermal products

• Florence Olechowski,
• Rainer H. Müller and
• Sung Min Pyo

Beilstein J. Nanotechnol. 2019, 10, 2152–2162, doi:10.3762/bjnano.10.208

• suspension was prepared containing 0.2% curcumin. Curcumin has many positive effects on the skin [31][32] and is at the same time fluorescent, allowing for a good and easy detection in the skin by fluorescence microscopy. The suspension was applied to pig ear skin in a covered Franz cell, incubated for 24 h

• and then skin slices were investigated by normal light and fluorescence microscopy (Figure 8, left column). Figure 8 (upper row) shows the fluorescence microscopy images. The lower row shows overlays of fluorescence and light microscopy images, allowing to locate the fluorescence in the epidermis. For

• . Penetration of curcumin into pig ear skin, vertical pig ear slices, fluorescence microscopy images (upper row) and overlay of fluorescence and light microscopy images (lower row); after application of 0.2% curcumin suspension in SmartLipids (left column), 2.0% curcumin micrometer-size crystal suspension

Nitrogen-vacancy centers in diamond for nanoscale magnetic resonance imaging applications

• Alberto Boretti,
• Lorenzo Rosa,
• Jonathan Blackledge and
• Stefania Castelletto

Beilstein J. Nanotechnol. 2019, 10, 2128–2151, doi:10.3762/bjnano.10.207

• illumination [7]. The characterization of single NV centers became popular at the end of the 1990s. It was demonstrated that the fluorescence of single NV centers can be detected by room-temperature fluorescence microscopy and that the defect shows perfect photostability [8]. Room-temperature optically

• allows real-time imaging of bacteria magnetosome chains with a direct correlation to the optical image. Single magnetosomes were thus resolved by the technique on a 100 μm wide field [52]. An alternative approach to fluorescence microscopy is magnetic imaging of cells, labeled or targeted, using MNPs

Gold-coated plant virus as computed tomography imaging contrast agent

• Alaa A. A. Aljabali,
• Mazhar S. Al Zoubi,
• Khalid M. Al-Batanyeh,
• Ali Al-Radaideh,
• Mohammad A. Obeid,
• Abeer Al Sharabi,
• Walhan Alshaer,
• Bayan AbuFares,
• Tasnim Al-Zanati,
• Murtaza M. Tambuwala,
• Naveed Akbar and
• David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

• −53.8 ± 2.4 mV upon antibody coating of the particles. The zeta potential value of VCAM1-PEG5000Au-CPMV becomes more negative [38], which is consistent with the literature [42]. In vitro fluorescent cell imaging Confocal fluorescence microscopy was performed on the cell lines to demonstrate the

• . Fluorescence microscopy confirmed that the VCAM1-PEG5000Au-CPMV can selectively bind to their target, whereas, the IgG-PEG5000Au-CPMV control did not show any fluorescence signal, which is indicative that no binding to the surface of the cells occurred (Figure 4A). The merged confocal microscopy image in

• holder. (C) UV–vis spectrum of the SPR bands of Au-CPMV; λmax = 535 nm for particles with a diameter of 50 nm (purple line), λmax = 552 nm for particles with a diameter of 70 nm (red line) and λmax = 572 nm for particles with a diameter of 100 nm (green line). Confocal fluorescence microscopy images of

Nanoarchitectonics meets cell surface engineering: shape recognition of human cells by halloysite-doped silica cell imprints

• Elvira Rozhina,
• Ilnur Ishmukhametov,
• Svetlana Batasheva,
• Farida Akhatova and
• Rawil Fakhrullin

Beilstein J. Nanotechnol. 2019, 10, 1818–1825, doi:10.3762/bjnano.10.176

• previously published protocol [24][25]. Silica/halloysite-decorated HeLa cells were then imaged in situ with optical fluorescence microscopy. A typical image is shown in Figure 2A demonstrating the preserved cell morphology and characteristic nuclear DAPI stain. Next, we imaged the silica/halloysite

• Milli-Q, and studied with AFM and SEM. Cells recognition by imprints The recognition of HeLa cells with imprints was visualised using bright-field optical microscopy (Axio Imager Z2, Carl Zeiss), and laser confocal microscopy (LSM 780: 405 nm and 633 nm lasers). For fluorescence microscopy imaging

• imprints and recognition of human cells by the imprints in HeLa/yeast cells mixture. The imprints were obtained by destruction of inorganic shells deposited on live cells. (A) optical/fluorescence microscopy image of live HeLa cells coated with halloysite-doped silica shells (nuclei are stained with DAPI

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• , when compared to the bare NPs. PLGA NPs preincubated with 500 µL FBS lead to the highest total red object area of 18.22 mm2/well. The results are consistent with the fluorescence microscopy images which were taken after an incubation time of 6 h (Figure 8). Furthermore, the images clearly demonstrate

• nanoparticle–cell interaction by fluorescence microscopy For fluorescence microscopy, HepG2 cells were seeded at a density of 3 × 104 cells/chamber on Millicell® EZ slides (Merck KGaA, Darmstadt, Germany) and cultivated overnight. Afterwards, the serum-containing medium was removed and cells were incubated

Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• of a silica-core doped with rhodamine to enable visualization via fluorescence microscopy, followed by a layer of PCL/ICG and a surface coating with either PCL or PLLA. Characterization of these particle types showed a size of 90 nm for PCL-NPs and 95 nm for PLLA-NPs. The zeta potential was −25.4 mV

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• morphology of the incubated cells were analysed with calcein acetoxymethyl ester (calcein-AM, Calbiochem, Schwalbach, Germany) fluorescence staining. For this, the cells were incubated with 1 μM calcein-AM for 30 min at 37 °C under cell culture conditions and subsequently analysed by fluorescence microscopy

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• undifferentiated cells, mesenchymal stem cells were utilized. Cellular uptake of agents was studied by fluorescence microscopy and induction of cell death was visualized by live/dead assay. Dox-conjugated γ-Fe2O3@P(HPMA-MMAA) particles showed enhanced cytotoxicity in drug-sensitive and drug-resistant tumor cells

• attached to the cells. The cellular uptake was measured by fluorescence microscopy with a Keyence Biorevo BZ-9000 instrument (Osaka, Japan) equipped with filters for Texas Red (EX 560/40, DM 585, BA 630/75) at 20× magnification. Dox and its complexes were visible as red fluorescing dots. Live/dead assay

• analyzed by fluorescence microscopy after 48 h of incubation with primary cells (hMSCs) and human tumor cells (MG-63 and HeLa); the nanoparticles were easily engulfed and well-accumulated in the target cells, but not in the hMSC cells (Figure 9). To better understand the cell-death mechanisms induced by

Non-agglomerated silicon–organic nanoparticles and their nanocomplexes with oligonucleotides: synthesis and properties

• Asya S. Levina,
• Marina N. Repkova,
• Nadezhda V. Shikina,
• Zinfer R. Ismagilov,
• Svetlana A. Yashnik,
• Dmitrii V. Semenov,
• Yulia I. Savinovskaya,
• Natalia A. Mazurkova,
• Inna A. Pyshnaya and
• Valentina F. Zarytova

Beilstein J. Nanotechnol. 2018, 9, 2516–2525, doi:10.3762/bjnano.9.234

• . Oligonucleotides in Si–NH2·ODN complexes retain their ability to form complementary duplexes. The Si–NH2Flu nanoparticles and Si–NH2·ODNFlu nanocomplexes were shown by fluorescence microscopy to penetrate into human cells. The Si–NH2Flu nanoparticles predominantly accumulated in the cytoplasm whereas ODNFlu

• (b). Fluorescence microscopy images of A549 human lung adenocarcinoma cells after their incubation with Si–NH2Flu nanoparticles and Si–NH2·ODN(3)Flu nanocomplexes. Fluorescein-labeled samples were detected in the green channel (488 nm) (a); DAPI-stained cell nuclei were detected in the blue channel

• second fraction (5% CH3CN). The mobility of the product in TLC (Rf ≈ 0.5) differed from that of hydrolyzed FITC (Rf ≈ 0.65). The concentration of Si–NH-Flu (33 µM) was evaluated spectrophotometrically using the molar absorption coefficient for fluorescein (ε495 = 74000 cm−1·M−1). For fluorescence

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

Electrospun one-dimensional nanostructures: a new horizon for gas sensing materials

• Muhammad Imran,
• Nunzio Motta and
• Mahnaz Shafiei

Beilstein J. Nanotechnol. 2018, 9, 2128–2170, doi:10.3762/bjnano.9.202

Optical near-field mapping of plasmonic nanostructures prepared by nanosphere lithography

• Gitanjali Kolhatkar,
• Alexandre Merlen,
• Jiawei Zhang,
• Chahinez Dab,
• Gregory Q. Wallace,
• François Lagugné-Labarthet and
• Andreas Ruediger

Beilstein J. Nanotechnol. 2018, 9, 1536–1543, doi:10.3762/bjnano.9.144

• with a femtosecond laser, thereby inducing polymerization at the hot spots. In another approach, based on confocal fluorescence microscopy, a dye solution is deposited on the samples, and the higher fluorescence magnitude originating from the hot spots is measured [19]. However, these last four

Nanoporous silicon nitride-based membranes of controlled pore size, shape and areal density: Fabrication as well as electrophoretic and molecular filtering characterization

• Axel Seidenstücker,
• Stefan Beirle,
• Fabian Enderle,
• Paul Ziemann,
• Othmar Marti and
• Alfred Plettl

Beilstein J. Nanotechnol. 2018, 9, 1390–1398, doi:10.3762/bjnano.9.131

• very low flow resistance of these porous membranes in ionic solutions as expected theoretically. Size-selective separation of protein molecules was demonstrated by real-time fluorescence microscopy. Keywords: ion transport; micellar technique; molecular filtration; nanopores; solid-state membrane

• well as FIB-drilled single pores is carried out. Subsequently, molecular filtering as a direct application of our nanoporous membranes is demonstrated: Successful size-selective separation of dye molecules and labeled proteins is observed by real-time fluorescence microscopy. Results and Discussion

• presented measurements of membranes with millions of pores. Permselectivity studies by fluorescence microscopy The main potential of nanoporous membranes will be the sieving of macromolecules in aqueous solution, especially for medical and biological use and even on an industrial scale [15][42][43

Room-temperature single-photon emitters in titanium dioxide optical defects

• Kelvin Chung,
• Yu H. Leung,
• Chap H. To,
• Aleksandra B. Djurišić and
• Snjezana Tomljenovic-Hanic

Beilstein J. Nanotechnol. 2018, 9, 1085–1094, doi:10.3762/bjnano.9.100

• characterisation measurements of fluorescence microscopy, correlation measurements, PL spectra and photodynamics are presented. Experimental Electron-beam deposition of TiO2 thin films The films were fabricated via e-beam deposition in high vacuum with the substrate temperature set to 200 °C during deposition

Wafer-scale bioactive substrate patterning by chemical lift-off lithography

• Chong-You Chen,
• Chang-Ming Wang,
• Hsiang-Hua Li,
• Hong-Hseng Chan and
• Wei-Ssu Liao

Beilstein J. Nanotechnol. 2018, 9, 311–320, doi:10.3762/bjnano.9.31

• fluorescence image represents a multiplexed surface spatially anchored with different probes including (I) Hg2+-specific, (II) adenosine-specific, and (III) cocaine-specific probes. The scale bar is 20 μm. Fluorescence microscopy images (A–H) of FAM-labelled DNA patterned surfaces with various features and

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• spectrometry, scintigraphic peritoneography and fluorescence microscopy. All experiments were performed contemporaneous with conventional PIPAC for the purpose of comparison. Furthermore, a first proof of concept was simulated in anesthetised German Landrace pigs. Results: HINAT provides a nanometre-sized (63

Patterning of supported gold monolayers via chemical lift-off lithography

• Liane S. Slaughter,
• Kevin M. Cheung,
• Sami Kaappa,
• Huan H. Cao,
• Qing Yang,
• Thomas D. Young,
• Andrew C. Serino,
• Sami Malola,
• Jana M. Olson,
• Stephan Link,
• Hannu Häkkinen,
• Anne M. Andrews and
• Paul S. Weiss

Beilstein J. Nanotechnol. 2017, 8, 2648–2661, doi:10.3762/bjnano.8.265

• the nanometer-scale heights of the supported Au monolayers through scanning probe microscopy, as well as the exploration of spatially encoded functionality using fluorescence microscopy. Otherwise, when topographically patterned PDMS stamps are used to pattern Au monolayers, the traits of the latter

• regions containing Au–alkanethiolates appeared bright in fluorescence microscopy images (Figure 2). Using this straightforward functionalization and visualization method, we investigated patterns of lifted-off Au monolayers on PDMS as substrates for DNA recognition. Upon hybridization of dye-labeled

• of the lifted-off monolayers were preserved from the Au-on-Si masters when patterning the lifted-off monolayers on PDMS, as determined by AFM and SEM imaging. These patterns of Au monolayers were recognizable in fluorescence microscopy when functionalized with thiolated DNA that was hybridized with

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• fluorescent dye (Hoechst 33232). Bright-field transmission images after fluo-CNO treatment confirmed that the HeLa cells were viable throughout all the experiments. From the fluorescence microscopy images (Figure 7), it was observed that cells treated with fluo-CNOs and maintained at a physiological pH (7.4

• 1 h, pH 4.5; Scale bars = 20 μm. Cellular uptake and localization of fluo-CNOs in HeLa cells in acidic conditions (PBS, pH 4.5) observed by confocal fluorescence microscopy after incubation for 2 h (A), 12 h (B) and 48 h (C), respectively. Scale bars = 20 μm. Three-dimensional reconstruction by

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• limitations of conventional fluorescence microscopy because FLIM results are insensitive to fluorophore concentration and excitation power of the laser. The growth of tumor in mice, inoculated with highly tumorigenic TC-1 cells immortalized using HPV type 16 proteins, was studied as a model of cervical cancer

• the challenge of reliable differentiation of all four types of cervical tissue by fluorescence endomicroscopy alone because of structural similarities of HSIL and stromal/columnar tissues in confocal endomicroscopic images. However, the capacity of confocal fluorescence microscopy to accurately

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• (UC) emission intensity of Tween 80-coated nanoparticles in comparison with oleic acid coated UCNPs. In addition, the nonspecific uptake and distribution of non-targeted Tween 80-coated UCNPs in human MCF-7 and MDB-MA-231 breast cancer cells was visualized by using confocal fluorescence microscopy

Collembola cuticles and the three-phase line tension

• Håkon Gundersen,
• Hans Petter Leinaas and
• Christian Thaulow

Beilstein J. Nanotechnol. 2017, 8, 1714–1722, doi:10.3762/bjnano.8.172

• the dye. The parts of the cuticle that were wetted by the dye can then be visualized with fluorescence microscopy. Results and Discussion Collembola cuticles were dyed with a water–acetone solution of Nile Red and imaged with fluorescence microscopy, a selection of cuticles are shown in Figure 4. The

• model of Zheng et al. [16] for . A system with θ0 = 105°, S = 0.1 μm and f = 0.25 is shown as a red line in each graph. Stained samples imaged with fluorescence microscopy, showing the tops of primary and (present in d and g) secondary granules. The light areas are those where the lipophilic dye has

• bonded with the surface, indicating wetting contact between the dye solution and a lipid layer. The images were obtained with confocal fluorescence microscopy using incidental light of 488 nm wavelength and a bandpass filter (565–615 nm). a) C. clavatus (winter-acclimated), b) C. clavatus (summer

Luminescent supramolecular hydrogels from a tripeptide and nitrogen-doped carbon nanodots

• Maria C. Cringoli,
• Slavko Kralj,
• Marina Kurbasic,
• Massimo Urban and
• Silvia Marchesan

Beilstein J. Nanotechnol. 2017, 8, 1553–1562, doi:10.3762/bjnano.8.157

• long term, a supramolecular hydrogel composed of a peptide and luminescent nanodots could be valuable for tissue regeneration based on a bioactive scaffold that can be also visualized in vivo by fluorescence microscopy. Alternatively, other potential applications could be developed in the future for

Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• observations, both using TEM and fluorescence microscopy (Supporting Information File 1, Figure S11). Not wishing to overstate these observations, such vesicular events may suggest rare events of transcytosis. Further studies need to be performed in order to fully address whether or not rare transcytosis

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

• Jennifer E. Francis,
• David Mason and
• Raphaël Lévy

Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125

• , United Kingdom 10.3762/bjnano.8.125 Abstract Semiconductor quantum dots (Qdots) have been utilised as probes in fluorescence microscopy and provide an alternative to fluorescent dyes and fluorescent proteins due to their brightness, photostability, and the possibility to excite different Qdots with a