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Search for "confocal microscopy" in Full Text gives 87 result(s) in Beilstein Journal of Nanotechnology.
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130
- depletion (STED) microscopy. Our approach allows for a step-by-step formation of dye-doped silica nanoparticles in the form of dye-incorporated spheres, which can be used as versatile fluorescent probes in confocal and STED imaging. Keywords: bioimaging; confocal microscopy; multistep synthesis approach
Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery
Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123
- by solubilizing the stain in 50 µL of 6 M guanidine hydrochloride (Sigma-Aldrich, USA) overnight at room temperature. Absorbance was measured at 620 nm directly in a 96-well plate using an Infinite 200 PRO plate reader and i-control software. Confocal microscopy For confocal microscopy analysis, 1
- Phalloidin Alexa Fluor488 (Thermo Fisher Scientific, USA) as previously described and analysed using confocal microscopy. Quantification of the QD fluorescent signal was achieved using Nis-Elements C 4.13 software. Single cell borders were defined according to the Phalloidin Alexa488 staining. The mean
Silicon microgrooves for contact guidance of human aortic endothelial cells
Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72
- statistically significant changes were observed. Cell adhesion The adhesion of HAECs on flat and grooved silicon substrates functionalized with collagen was assessed with ESEM and confocal microscopy after two days of culture. The results revealed that the number of adhered cells on groove substrates tended to
- be different to that on flat substrate. Compared to the flat substrate, the number of cells adhered was lower in slope-shaped grooves and higher in V-shaped grooves irrespective of the ridge width (Figure 4a). Moreover, confocal microscopy images provide also information on the location of cells in
- ), as described further below. Staining on actin and nuclei and fluorescence confocal microscopy HAECs were cultured on the functionalized substrates for 2 and 7 days. After cell culture experiments, culture media were removed and cells were washed twice with PBS at 37 °C and afterwards fixed as
Copper atomic-scale transistors
Beilstein J. Nanotechnol. 2017, 8, 530–538, doi:10.3762/bjnano.8.57
- confocal microscopy image shown in Figure 7a was taken during the initial point-contact formation between source and drain. By comparing Figure 7a with Figure 4a we conclude, that the additives strongly influence the morphology of the deposited copper film and result in lower roughness. The sample used in
- -scale transistor. Confocal microscopy images taken before electrochemical copper deposition, after deposition, and during transistor operation are shown (from left to right). The operation of a copper atomic-scale transistor driven by a function generator. (a) Schematic diagram of the function-generator
Active and fast charge-state switching of single NV centres in diamond by in-plane Al-Schottky junctions
Beilstein J. Nanotechnol. 2016, 7, 1727–1735, doi:10.3762/bjnano.7.165
- centre sheet density in of [NV] = 106–107 cm−2. We used confocal microscopy with an excitation laser of 520 nm to detect the NV centre photoluminescence in the depletion region of the Schottky junction. Distinct spectral characteristics can be used to identify the charge state: the NV+ centre does not
Fabrication of hybrid graphene oxide/polyelectrolyte capsules by means of layer-by-layer assembly on erythrocyte cell templates
Beilstein J. Nanotechnol. 2015, 6, 2310–2318, doi:10.3762/bjnano.6.237
- and show the presence and location of the GO by Raman confocal microscopy. Another potential application of these capsules is for the production of PE/GO films with a high concentration of GO, which was achievable in the present study by employing a high concentration of exfoliated GO during the layer
PLGA nanoparticles as a platform for vitamin D-based cancer therapy
Beilstein J. Nanotechnol. 2015, 6, 1306–1318, doi:10.3762/bjnano.6.135
- calcitriol remained stable at release conditions throughout the experiment period. Cellular uptake of PLGA NPs and calcitriol-induced morphological changes The internalization of fluorescent C6–calcitriol–PLGA NPs by S2-013, hTERT-HPNE and A549 cells was evaluated by confocal microscopy. Counterstaining of
- experiments were performed in triplicate. Cellular imaging studies Laser scanning confocal microscopy (LSCM) was used to evaluate the NP in vitro uptake and morphological changes in S2-013, hTERT-HPNE and A549 cells. The cells were seeded in µ-chamber 12-well plates (ibidi, Germany) at a density of 1000 cells
Protein corona – from molecular adsorption to physiological complexity
Beilstein J. Nanotechnol. 2015, 6, 857–873, doi:10.3762/bjnano.6.88
- confocal microscopy. In these experiments, all QDs were found to accumulate at the cell membrane within minutes after exposure. However, the kinetic analysis of this process showed characteristic times for QD association to the membrane to differ by more than one order of magnitude. Rate coefficients were
In situ observation of biotite (001) surface dissolution at pH 1 and 9.5 by advanced optical microscopy
Beilstein J. Nanotechnol. 2015, 6, 665–673, doi:10.3762/bjnano.6.67
- by employing laser confocal microscopy with differential interference contrast microscopy (LCM-DIM, Figure 6a). This advanced optical system is a combination of two microscopy techniques: a confocal system (FV300, Olympus) is attached to an inverted optical microscope (IX70, Olympus) and a Nomarski
Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development
Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66
- µg/mL) showed no effect on the oocyte maturation (Figure 5). Despite the lack of toxicity, the particles were found to have been internalized in great numbers into the oocytes as observed by confocal microscopy (Figure 6). The directly adjacent cumulus cells incorporated gold nanoparticles to a
Silica micro/nanospheres for theranostics: from bimodal MRI and fluorescent imaging probes to cancer therapy
Beilstein J. Nanotechnol. 2015, 6, 546–558, doi:10.3762/bjnano.6.57
- cytometry, confocal microscopy and MRI studies suggested that the prepared nanocomposites can be used for targeting cancer cells that overexpress folic acid. Similar strategies were also used by Peng et al. [22] by using an iridium(III) complex as fluorescent agent. Hu et al. [23] reported the synthesis of
- TEM analysis. It was observed that after the addition of QDs, the average diameter of the particles increased by about 20 nm. The silica-coated QDs showed a red shift of 5 nm in the emission spectra when these were compared with free QDs. The confocal microscopy images of CHO cells treated with hybrid
- with folic acid for targeting two different pancreatic cancer cell lines, namely PANC-1 and BxPC3. The confocal microscopy studies suggested that the nanocomposites were indeed internalized by the cells and not simply bound on the surface membrane. The T2-weighted images suggested that the NPs can be
Overview about the localization of nanoparticles in tissue and cellular context by different imaging techniques
Beilstein J. Nanotechnol. 2015, 6, 263–280, doi:10.3762/bjnano.6.25
- life science applications but not in routine toxicopathology so far. FLIM setups usually combine conventional laser scanning confocal microscopy with time-correlated single photon counting, thus, enabling the recording of fluorescence lifetime decay traces for each pixel. The fluorescence lifetime
Tailoring the ligand shell for the control of cellular uptake and optical properties of nanocrystals
Beilstein J. Nanotechnol. 2015, 6, 232–242, doi:10.3762/bjnano.6.22
- viability of the cells [25][30]. The properties of a representative selection concerning the cellular uptake was investigated by incubating A549 cells under biologically relevant conditions for up to 4 h and under more radical conditions in serum free media for 20 h and analyzed via confocal microscopy. The
- samples, bearing the amino functions showed no unspecific interaction with the cell membrane, which qualifies the nanocontainers in a first step as versatile tools for specific targeting, since no unspecific background has to be expected. Figure 10 shows exemplarily the confocal microscopy images for the
- . Different applied monomers for the functionalization during the seeded emulsion polymerization (upper part); strategy for the introduction of a PEG linker between the functional group or the functional molecule and a polymerizable alkene function (lower part) [40]. Confocal microscopy images of A549 cells
Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells
Beilstein J. Nanotechnol. 2015, 6, 167–176, doi:10.3762/bjnano.6.16
- . The iron content of the samples was then determined by a photometric assay (Spectroquant®, Merck) and by ICP–MS (iCAP 6000, Thermo Scientific). Experiments were repeated three times. Fluorescence microscopy Spinning disk confocal microscopy was used to detect SCIONs inside cells. The applied system
Increasing throughput of AFM-based single cell adhesion measurements through multisubstrate surfaces
Beilstein J. Nanotechnol. 2015, 6, 157–166, doi:10.3762/bjnano.6.15
- coating was not demonstrated, we coated PDMS wells with fluorescently labeled BSA (FITC), collagen I (FITC), fibronectin (rhodamine) and laminin (HiLyte488) and imaged the glass (E–H, respectively) and PDMS (E’–F’) surfaces. Using confocal microscopy, a 20 × 20 µm2 area was bleached with maximum laser
The distribution and degradation of radiolabeled superparamagnetic iron oxide nanoparticles and quantum dots in mice
Beilstein J. Nanotechnol. 2015, 6, 111–123, doi:10.3762/bjnano.6.11
- the Zn pool was observed. Confocal microscopy of rat liver cryosections (prepared 2 h after intravenous injection of polymer-coated Qdots) revealed a colocalization with markers for Kupffer cells and liver sinusoidal endothelial cells (LSEC), but not with hepatocytes. In J774 macrophages, fluorescent
- < 0.05), indicating an incomplete degradation of particles in the liver. Confocal microscopy of a cryosection of a rat liver 2h after intravenous injection of polymer-coated Qdots. The nuclei are stained with DAPI. Immunostaining of Kupffer cells (KCs, anti-CD31) and liver sinusoidal endothelial cells
Synthesis and characterization of fluorescence-labelled silica core-shell and noble metal-decorated ceria nanoparticles
Beilstein J. Nanotechnol. 2014, 5, 2413–2423, doi:10.3762/bjnano.5.251
- As principal means of investigation by our physicochemical and medicinal partners, confocal microscopy and other fluorescence-based methods were envisaged. Consequently, a proper choice of the fluorescent label is crucial. We first experimented with commercial dyes Cy3 and Cy5 having an emission in a
Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions
Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250
- new drug delivery systems and predict functionalization-dependent health hazards that nanoparticles might exhibit. Polystyrene synthesis. Spinning disc confocal microscopy of acute monocytic leukemia THP-1 cells, differentiated THP-1 cells, and human macrophages. Cell membranes are stained with
- -COOH or PS-NH2 nanoparticles (each 100 µg/mL) and analyzed by flow cytometry or confocal microscopy (24 h). Results are given as mean ± SEM, n = 3, *p < 0.05, **p < 0.01. Nuclei are labeled with HCS NuclearMask™ (blue), nanoparticles are stained with PMI (green), lysosomes are labeled with LysoTracker
Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects
Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248
- . By using spinning disk confocal microscopy in combination with quantitative image analysis, we studied the time courses of NP association with the cell membrane and subsequent internalization. NPs with diameters of less than 10 nm were observed to accumulate at the plasma membrane before being
- capable of providing enough ligand–receptor binding strength to initiate internalization. As observed with our experiments (Figure 4), accumulation on the membrane is not required. Conclusion By using spinning disk confocal microscopy, we have systematically investigated the in situ internalization
- ; cationic and NPS, 7.5 µg/mL). AuNCs were suspended in serum-free DMEM at 20 µg/mL. Live cell imaging was performed for up to 2 h with our spinning disk laser scanning confocal microscopy systems [29][31]. Images were acquired in two separate color channels. NP emission was collected through a bandpass
Interaction of dermatologically relevant nanoparticles with skin cells and skin
Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245
- that enable studies on the whole skin, ideally under in vivo conditions, e.g., in vivo confocal microscopy and multiphoton microscopy [9]. In earlier studies using mice, we were able to monitor the penetration of fluorescent 200 nm particles in hair follicles and diffusion into perifollicular tissues
Anticancer efficacy of a supramolecular complex of a 2-diethylaminoethyl–dextran–MMA graft copolymer and paclitaxel used as an artificial enzyme
Beilstein J. Nanotechnol. 2014, 5, 2293–2307, doi:10.3762/bjnano.5.238
- cellular distribution of micelles is required to enable the selective delivery of a drug to a specific target at the subcellular level [4]. By means of triple-labeling confocal microscopy of living cells, Savic et al. identified the exact cellular targets of block copolymer micelles, i.e., several
Nanomanipulation and environmental nanotechnology
Beilstein J. Nanotechnol. 2014, 5, 2079–2080, doi:10.3762/bjnano.5.216
- based on polyethylene oxide. The surface reactivity of minerals in contact with aqueous solutions can be investigated by laser confocal microscopy, as shown on the example of dissolution of the mineral biotite in solutions with acid and basic pH. Recent nanofiltration techniques are reviewed with
Carbon nano-onions (multi-layer fullerenes): chemistry and applications
Beilstein J. Nanotechnol. 2014, 5, 1980–1998, doi:10.3762/bjnano.5.207
- 2011 John Wiley and Sons. High-resolution TEM images of pristine CNOs (left). AFM topographs of pristine CNOs deposited on mica (center). Confocal microscopy of C57BL/6 BMDCs incubated in the presence of fluorescein-labelled-CNOs and stained with wheat germ agglutinin-Alexa Fluor594 (red), fluorescein
Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183
- -Fe2O3. The beneficial effect of coating (diminishing particle cytotoxic activity for the cells) was thus demonstrated. By using confocal microscopy, D-mannose- and PDMAAm-coated γ-Fe2O3 nanoparticles were detected in vacuoles inside the cytoplasm of the cells (Figure 3a,c). PDMAAm-coated particles were
Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy
Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173
- are normally assessed via qualitative and semi-quantitative analyses of images. The need for a method to rapidly quantify the absolute number of nanoparticles internalized by cells led us to the development of a highly innovative method that integrates high resolution confocal microscopy with
- measured through confocal microscopy in a time series between 1 and 24 h. The concentration of nanoparticles was 39.5 µg·mL−1 (or 30000 nanoparticles per cell) in all experiments. We found that within the first 4 h of incubation the number of intracellular particles was up to 10 times higher for HUVEC than
- 417 nm and 316 nm agglomerates, respectively. Nanoparticles at a concentration of 10 µg·mL−1 were incubated with human microvascular endothelial cells (HMEC-1) for 3, 24, 48 and 72 h and imaged through live-cell confocal microscopy. Cytotoxicity assays performed on similar nanoparticles have shown
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