Selective detection of Mg²⁺ ions via enhanced fluorescence emission using Au–DNA nanocomposites

• Tanushree Basu,
• Khyati Rana,
• Niranjan Das and
• Bonamali Pal

Beilstein J. Nanotechnol. 2017, 8, 762–771, doi:10.3762/bjnano.8.79

• bromide (CTAB) and DL-dithiothreitol solution (DTT) were purchased from Sigma Aldrich. Sodium borohydride (NaBH4) and silver nitrate (AgNO3) were purchased from Rankem and Fisher Scientific, respectively. Chloroauric acid (HAuCl4·H2O), ascorbic acid, mercaptopropionic acid (MPA) and magnesium acetate (Mg

α-((4-Cyanobenzoyl)oxy)-ω-methyl poly(ethylene glycol): a new stabilizer for silver nanoparticles

• Jana Lutze,
• Miguel A. Bañares,
• Marcos Pita,
• Andrea Haase,
• Andreas Luch and
• Andreas Taubert

Beilstein J. Nanotechnol. 2017, 8, 627–635, doi:10.3762/bjnano.8.67

• -ascorbic acid (≥99%, Roth), N,N’-dicyclohexylcarbodiimide (99%, Aldrich), poly(ethylene glycol) methyl ether (Mn = 4830 g·mol−1, Aldrich), silver nitrate (≥99%, Sigma-Aldrich), sodium hydroxide (>99%, Roth), and trisodium citrate dihydrate (99.7%, Merck) were used as received. Water with a resistivity of

• 0.5 M sodium hydroxide solution. The mixture was heated to 30 °C and a silver nitrate solution (0.1 M, 80 µL, 8.0 µmol) was added under vigorous stirring. After ten minutes stirring at room temperature the mixture was refluxed for two hours, resulting in an orange dispersion, which was allowed to cool

Comparison of four methods for the biofunctionalization of gold nanorods by the introduction of sulfhydryl groups to antibodies

• Xuefeng Wang,
• Zhong Mei,
• Yanyan Wang and
• Liang Tang

Beilstein J. Nanotechnol. 2017, 8, 372–380, doi:10.3762/bjnano.8.39

• trihydrate (HAuCl4·3H2O; 99%), cetyltrimethylammonium bromide (CTAB), sodium borohydride (NaBH4; 99%), silver nitrate (AgNO3; 99%), L-ascorbic acid (AA), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimidehydrochloride (EDC), sodium oleate (NaOL; >97%), hydrochloric acid (HCl, 37%), (3-mercaptopropyl

Microfluidic setup for on-line SERS monitoring using laser induced nanoparticle spots as SERS active substrate

• Oana-M. Buja,
• Ovidiu D. Gordan,
• Nicolae Leopold,
• Andreas Morschhauser,
• Jörg Nestler and
• Dietrich R. T. Zahn

Beilstein J. Nanotechnol. 2017, 8, 237–243, doi:10.3762/bjnano.8.26

• continuous flow of a mixture of silver nitrate or gold chloride and sodium citrate. The described microfluidic setup enables within a few minutes the monitoring of several processes: the synthesis of the SERS active spot, MG adsorption to the metal surface, detection of the analyte when saturation of the

• detection of the analyte, as shown schematically in Figure 1a. SERS monitoring of MG adsorbed on silver nanoparticle spots The synthesis of the SERS active silver nanoparticles spot was carried out by laser-induced spot synthesis [24] inside the glass capillary. The silver nitrate and sodium citrate

• beam (λ = 514.7 nm) on 5 μL of silver/citrate mixture for 60 s. The scanning electron microscopy (SEM) image was obtained with a FEI NovaNanoSEM 200 scanning electron microscope with a working distance of 5.9 mm. The following chemical reagents were used: silver nitrate (Alfa Aesar), hydrogen

Hydrophilic silver nanoparticles with tunable optical properties: application for the detection of heavy metals in water

• Paolo Prosposito,
• Federico Mochi,
• Erica Ciotta,
• Mauro Casalboni,
• Fabio De Matteis,
• Iole Venditti,
• Laura Fontana,
• Giovanna Testa and
• Ilaria Fratoddi

Beilstein J. Nanotechnol. 2016, 7, 1654–1661, doi:10.3762/bjnano.7.157

• procedure (synthesis b) for the synthesis of the AgNPs. In this case, the molar ratio between silver nitrate, sodium borohydride and 3-MPS was varied. The reaction was conducted at 3 °C and the final purification was avoided in order to prevent possible aggregation with a consequent broadening of the SPR

• the development of an analytical sensor system based on optical methods to detect metal ions in actual water samples. Experimental Materials and sample preparation Silver nitrate (AgNO3, Sigma-Aldrich, 99.5%), sodium borohydride (NaBH4, Sigma-Aldrich, 98%) and 3-mercapto-1-propanesulfonic acid sodium

• water several times (10 min, 15 000 rpm). Synthesis b: sodium borohydride and silver nitrate solution (in deionized water) was mixed with concentration ratio NaBH4/AgNO3 2:1. The NaBH4 solution was cooled to 3 °C under vigorous stirring, then the AgNO3 solution was added dropwise at approximately one

Templated green synthesis of plasmonic silver nanoparticles in onion epidermal cells suitable for surface-enhanced Raman and hyper-Raman scattering

• Marta Espina Palanco,
• Klaus Bo Mogensen,
• Marina Gühlke,
• Zsuzsanna Heiner,
• Janina Kneipp and
• Katrin Kneipp

Beilstein J. Nanotechnol. 2016, 7, 834–840, doi:10.3762/bjnano.7.75

• silver nitrate solution (10−3 M concentration from 99.9% pure AgNO3, Sigma-Aldrich Denmark A/S). After 20 h of incubation at room temperature and in darkness, the pieces were removed, rinsed with tap water and placed on a glass slide to dry for several hours, also in darkness. After drying, the samples

Controlled graphene oxide assembly on silver nanocube monolayers for SERS detection: dependence on nanocube packing procedure

• Martina Banchelli,
• Bruno Tiribilli,
• Roberto Pini,
• Luigi Dei,
• Paolo Matteini and
• Gabriella Caminati

Beilstein J. Nanotechnol. 2016, 7, 9–21, doi:10.3762/bjnano.7.2

• produces a large fraction of particle clusters with small interparticle distance, which generates an efficient hot spot distribution. Experimental Materials. Ethylene Glycol (EG, ≥99%) was obtained from Scharlab. Sodium sulfide nonahydrate, PVP (Mw = 55000), silver nitrate and GO solution (4 mg mL−1) were

• . Then, 0.175 mL of a 0.72 mg mL−1 sodium sulfide solution and 3.75 mL of a 20 mg mL−1 PVP solution in EG were subsequently added to the flask. The flask was thermostated for additional 10 min, until a temperature of 150 °C was again established. A silver nitrate solution (1.25 mL) in EG with a

Ultrastructural changes in methicillin-resistant Staphylococcus aureus induced by positively charged silver nanoparticles

• Dulce G. Romero-Urbina,
• Humberto H. Lara,
• J. Jesús Velázquez-Salazar,
• M. Josefina Arellano-Jiménez,
• Eduardo Larios,
• Anand Srinivasan,
• Jose L. Lopez-Ribot and
• Miguel José Yacamán

Beilstein J. Nanotechnol. 2015, 6, 2396–2405, doi:10.3762/bjnano.6.246

• widely known. Around the 1800s silver nitrate was commonly applied topically to treat burns and ulcerations or infected wounds, although its use declined following the introduction of antibiotics. Fox revived its use in the form of silver sulfadiazine, which is applied topically in burn therapy [23]. An

• + ion leaching. Therefore, the combination of all three species is more efficient in binding and lysing bacteria. Experimental Chemicals and Materials: Silver nitrate, AgNO3 (99.99%), was purchased from Sigma-Aldrich and used as received. Distilled water was purified using Whatman® 0.2 µm filters. A

• film for further analysis. The use of microwaves to synthesize silver nanoparticles has been shown to work in the presence of an eco-friendly reducing agent [54]. Silver nitrate can decompose into metallic silver, NO2 gas and O2 by the addition of heat as represented in Equation 1 [55]: In our study

In situ SU-8 silver nanocomposites

• Søren V. Fischer,
• Basil Uthuppu and
• Mogens H. Jakobsen

Beilstein J. Nanotechnol. 2015, 6, 1661–1665, doi:10.3762/bjnano.6.168

• the composite matrix. At higher precursor concentrations, larger agglomerated NPs are dominant and large islands of phase separated Ag are formed in the composite. Experimental Preparation of Ag NPs in cyclopentanone Materials Silver nitrate (AgNO3, ≥99.0%) and sodium borohydride (NaBH4, ≥98.0%) was

• bought from Sigma-Aldrich. Luviskol® VA 64, a poly(vinylpyrrolidone-co-vinyl acetate) (PVP/VA) mixture was kindly gifted by BASF. Method 1 g of PVP/VA and 0.1 g of silver nitrate is dissolved in 50 mL of absolute ethanol. 0.02 g of sodium borohydride and 0.2 g of PVP/VA dissolved in 10 mL of absolute

Formation of substrate-based gold nanocage chains through dealloying with nitric acid

• Ziren Yan,
• Ying Wu and
• Junwei Di

Beilstein J. Nanotechnol. 2015, 6, 1362–1368, doi:10.3762/bjnano.6.140

• Suzhou NSG Electronics Co. Ltd. (Suzhou, China). Prior to use, it was divided into the strips (50 × 6 mm). All chemicals were of analytical grade and used as received without any further purification. Silver nitrate (AgNO3), hydrogen tetrachloroaurate (HAuCl4), nitric acid (HNO3), hydrogen peroxide (H2O2

Synthesis, characterization and in vitro effects of 7 nm alloyed silver–gold nanoparticles

• Simon Ristig,
• Svitlana Chernousova,
• Wolfgang Meyer-Zaika and
• Matthias Epple

Beilstein J. Nanotechnol. 2015, 6, 1212–1220, doi:10.3762/bjnano.6.124

• ablation was reported [25][26][27]. Alloying of presynthesized silver core/gold shell nanoparticles by refluxing with oleylamine [28] or ultrasonication of separate gold and silver nanoparticles [29] was also described. Here, an aqueous co-reduction of silver nitrate and tetrachloroauric acid with a

• is possible that a passivating effect from the alloyed gold is responsible for these observations. Future studies on the time-dependent dissolution of such alloyed nanoparticles in biological media may help to better understand this effect. Experimental Chemicals We used silver nitrate (Roth, p.a

Influence of gold, silver and gold–silver alloy nanoparticles on germ cell function and embryo development

• Ulrike Taylor,
• Daniela Tiedemann,
• Christoph Rehbock,
• Wilfried A. Kues,
• Stephan Barcikowski and
• Detlef Rath

Beilstein J. Nanotechnol. 2015, 6, 651–664, doi:10.3762/bjnano.6.66

• . Shown are percentage of spermatozoa, which differ compared to the control [values are mean ± SD]. Reproduced with permission from [50]. Copyright 2014 Royal Society of Chemistry. Oocyte maturation rates after 46 h of in vitro maturation in the presence of various nanoparticle types or silver nitrate in

• displayed sperm section in total. Above each section the relevant nanoparticle type is displayed schematically. PM = plasma membrane; Ac = acrosome; Nu = nucleus (adapted from [49][50]). Sperm viability parameters after co-incubation of sperm for 2 h at 37 °C with various nanoparticle types and a silver

• nitrate control. Nanoparticle concentration was 10 µg/mL. (A) Motility assessed with Computer Assissted Sperm Analysis, (B) Membrane integrity assessed with propidium iodide stain and flow cytometer, (C) morphology assessed with phase contrast microscope and evaluation of 200 sperm cells per group per day

Novel ZnO:Ag nanocomposites induce significant oxidative stress in human fibroblast malignant melanoma (Ht144) cells

• Syeda Arooj,
• Samina Nazir,
• Akhtar Nadhman,
• Nafees Ahmad,
• Bakhtiar Muhammad,
• Ishaq Ahmad,
• Kehkashan Mazhar and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2015, 6, 570–582, doi:10.3762/bjnano.6.59

• ), pyruvic acid, silver nitrate, NaN3, sodium chloride, sodium dodecyl sulfate (SDS), sodium hydroxide (NaOH), sodium sarcosinate, streptomycin sulfate, sulforhodamine B (SRB), 1,1,3,3-tetramethoxypropane, MTT, thiobarbituric acid (TBA), trichloroacetic acid (TCA), Triton X-100, trizma-Base, trypsin/EDTA (5

• reported procedure with some modifications [29]. Briefly, zinc nitrate hexahydrate and the required amount of silver nitrate (1, 3, 5, 10, 20 and 30 mol %) were dissolved in 5% v/v Tween 80 to achieve 50 mM concentration. The resulting substrate solution was titrated against 100 mM NaOH through a drop-wise

Green preparation and spectroscopic characterization of plasmonic silver nanoparticles using fruits as reducing agents

• Jes Ærøe Hyllested,
• Marta Espina Palanco,
• Nicolai Hagen,
• Klaus Bo Mogensen and
• Katrin Kneipp

Beilstein J. Nanotechnol. 2015, 6, 293–299, doi:10.3762/bjnano.6.27

• capability to reduce silver and gold salts and to create silver and gold nanoparticles. We report the preparation of silver nanoparticles with sizes between 10 and 300 nm from silver nitrate using fruit extract collected from pineapples and oranges as reducing agents. The evolvement of a characteristic

• surface plasmon extinction spectrum in the range of 420 nm to 480 nm indicates the formation of silver nanoparticles after mixing silver nitrate solution and fruit extract. Shifts in plasmon peaks over time indicate the growth of nanoparticles. Electron microscopy shows that the shapes of the

• enhanced Raman scattering (SERS). Extracts from these two fruits have been used for preparing silver and gold nanoparticles [12][15][16][17][18][19]. Here we explore the formation of nanoparticles by varying conditions in the preparation process such as ratios of the mixtures of silver nitrate and fruit

Mechanical properties of MDCK II cells exposed to gold nanorods

• Anna Pietuch,
• Bastian Rouven Brückner,
• David Schneider,
• Marco Tarantola,
• Christina Rosman,
• Carsten Sönnichsen and
• Andreas Janshoff

Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21

• mL of 0.1 M CTAB, 7 μL of 0.04 M silver nitrate (AgNO3), and 105 μL of 0.08 M ascorbic acid. Nanoparticle size was controlled by transmission electron microscopy (TEM). We determined a length of 38 ± 6.5 nm and a width of 17 ± 3 nm for nanorod and a diameter of 43 nm for spheres [25]. Concentrations

Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating

• Christina Rosman,
• Sebastien Pierrat,
• Marco Tarantola,
• David Schneider,
• Eva Sunnick,
• Andreas Janshoff and
• Carsten Sönnichsen

Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257

• solution consisting of HAuCl4 (75 µL, 0.1 M), CTAB (10 mL, 0.1 M), silver nitrate (AgNO3, 7 µL, 0.04 M), and ascorbic acid (AA, 105 µL, 0.0788 M). Shortly before the experiment, the nanoparticles were washed in two centrifugation steps with water to remove unbound CTAB. Particle characterization Particles

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• dihydrate (Fluka, p.a.), silver nitrate (Fluka, p.a.), and D-(+)-glucose (Baker) were used. Ultrapure water was prepared with an ELGA Purelab ultra instrument. Ag-NP were stored under argon to prevent partial oxidative dissolution (which drastically influences nanoparticle toxicity) prior to cell culture

PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments

• Sebastian Ahlberg,
• Alexandra Antonopulos,
• Jörg Diendorf,
• Ralf Dringen,
• Matthias Epple,
• Rebekka Flöck,
• Wolfgang Goedecke,
• Christina Graf,
• Nadine Haberl,
• Jens Helmlinger,
• Fabian Herzog,
• Frederike Heuer,
• Stephanie Hirn,
• Christian Johannes,
• Stefanie Kittler,
• Manfred Köller,
• Katrin Korn,
• Wolfgang G. Kreyling,
• Fritz Krombach,
• Jürgen Lademann,
• Kateryna Loza,
• Eva M. Luther,
• Marcelina Malissek,
• Martina C. Meinke,
• Daniel Nordmeyer,
• Anne Pailliart,
• Jörg Raabe,
• Fiorenza Rancan,
• Barbara Rothen-Rutishauser,
• Eckart Rühl,
• Carsten Schleh,
• Andreas Seibel,
• Christina Sengstock,
• Lennart Treuel,
• Annika Vogt,
• Katrin Weber and
• Reinhard Zellner

Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205

• silver nanoparticles with defined shapes and sizes is extensively described in the literature, with more than 50 publications alone by the group of Xia et al. [21]. The most common and best examined method is the polyol process during which an ionic silver salt (typically silver nitrate or silver

• quantities over a large number of experiments. Because of this, we decided to synthesize our particles by the reduction of silver nitrate with glucose in the presence of PVP according to Wang et al. [28]. This leads to high yields of spherical silver nanoparticles with diameters of around 70–120 nm and a few

• nanoparticles neither caused toxicity nor oxidative stress, while an incubation for 4 h with 100 µM (10.8 µg mL−1) silver in the form of silver nitrate strongly damaged cultured astrocytes and deprived these cells almost completely of the important antioxidant glutathione [108]. The high resistance of cultured

Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity

• Dickson Joseph,
• Nisha Tyagi,
• Christian Geckeler and
• Kurt E.Geckeler

Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158

• and drug delivery due to their pH sensitivity, high stability and biocompatible surfaces. Experimental Materials and characterization Potassium tetrachloroaurate (KAuCl4) was purchased from Aldrich. Silver nitrate (AgNO3) was obtained from Kojima Chemicals Co. Ltd., Korea. All of the proteins, calf

Mimicking exposures to acute and lifetime concentrations of inhaled silver nanoparticles by two different in vitro approaches

• Fabian Herzog,
• Kateryna Loza,
• Sandor Balog,
• Martin J. D. Clift,
• Matthias Epple,
• Peter Gehr,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2014, 5, 1357–1370, doi:10.3762/bjnano.5.149

• weight 40,000 g/mol), silver nitrate (Roth, p.a.), and D-(+)-glucose (Sigma-Aldrich) were used. Ultrapure water was prepared with an ELGA Purelab ultra instrument. Suspensions for exposure were adjusted to 24, 240 and 2400 µg Ag/mL by dilution in double-distilled H2O or via ultrafiltration by using 30

Self-organization of mesoscopic silver wires by electrochemical deposition

• Sheng Zhong,
• Thomas Koch,
• Stefan Walheim,
• Harald Rösner,
• Eberhard Nold,
• Aaron Kobler,
• Torsten Scherer,
• Di Wang,
• Christian Kübel,
• Mu Wang,
• Horst Hahn and
• Thomas Schimmel

Beilstein J. Nanotechnol. 2014, 5, 1285–1290, doi:10.3762/bjnano.5.142

• electrochemical environments as well as for the fabrication of highly-ordered, single-crystalline metal nanowires. Keywords: crystal growth; electrochemistry; electrodeposition; mesowires; nanoelectrochemistry; nanowires; self-organization; silver nanowires; silver nitrate; stability; Introduction Nanoscale and

• measurements presented in this article). STEM micrographs were acquired using an HAADF (High-Angle Annular Dark-Field) detector. Schematic diagrams of the experimental procedure. (a) By slowly freezing the silver nitrate electrolyte a thin aqueous layer of electrolyte forms between the glass slide and the ice

Injection of ligand-free gold and silver nanoparticles into murine embryos does not impact pre-implantation development

• Ulrike Taylor,
• Wiebke Garrels,
• Annette Barchanski,
• Svea Peterson,
• Laszlo Sajti,
• Andrea Lucas-Hahn,
• Lisa Gamrad,
• Ulrich Baulain,
• Sabine Klein,
• Wilfried A. Kues,
• Stephan Barcikowski and
• Detlef Rath

Beilstein J. Nanotechnol. 2014, 5, 677–688, doi:10.3762/bjnano.5.80

• were cultured in M16 medium (Sigma-Aldrich) at 37 °C in a humidified incubator with 5% CO2 in air for 3 days. In order to distinguish whether possible effects are caused by the nanoparticles as such or by Ag+-ions released from the nanoparticles, additional embryos were co-incubated with 25 µM silver

• nitrate. To exclude the influence of the NO3−-ions on the embryo development 25 µM potassium nitrate controls were also run. Embryo development was assessed on a daily basis and documented by using a stereo-microscope (Olympus SZX16, Olympus, Hamburg, Germany) equipped with a camera (Olympus DP72, Olympus

Enhanced photocatalytic activity of Ag–ZnO hybrid plasmonic nanostructures prepared by a facile wet chemical method

• Sini Kuriakose,
• Vandana Choudhary,
• Biswarup Satpati and
• Satyabrata Mohapatra

Beilstein J. Nanotechnol. 2014, 5, 639–650, doi:10.3762/bjnano.5.75

• ) were used as the starting materials for the synthesis of ZnO nanostructures. Silver nitrate (AgNO3, Spectrochem, India) and trisodium citrate (Na3C6H5O7, CDH, India) were used for the photodeposition of Ag nanoparticles onto ZnO nanostructures. Methylene blue (MB, SRL India) was used as dye for

• continuously stirred overnight. Silver nitrate of concentrations varying from 0.1 to 2 mM was added into these solutions under stirring for 30 min in the dark. The [Ag+]/[citrate] concentration ratios in these solutions were chosen to be 1:1 and 1:10 for different AgNO3 concentrations. Photoreduction of Ag

In vitro toxicity and bioimaging studies of gold nanorods formulations coated with biofunctional thiol-PEG molecules and Pluronic block copolymers

• Tianxun Gong,
• Douglas Goh,
• Malini Olivo and
• Ken-Tye Yong

Beilstein J. Nanotechnol. 2014, 5, 546–553, doi:10.3762/bjnano.5.64

• have minimal cytotoxicity and they can be used for long term in vitro and in vivo imaging study. Experimental Materials: Hydrogen tetrachloroaurate(III) trihyrate (HAuCl4·3H2O), cetylmethylammonium bromide (CTAB), sodium borohydride (NaBH4), silver nitrate (AgNO3), L-ascorbic acid, trisodium citrate

One pot synthesis of silver nanoparticles using a cyclodextrin containing polymer as reductant and stabilizer

• Arkadius Maciollek and
• Helmut Ritter

Beilstein J. Nanotechnol. 2014, 5, 380–385, doi:10.3762/bjnano.5.44

• using silver nitrate and a copolymer 1 from N-isopropylacrylamide (NIPAM) and mono-(1H-triazolylmethyl)-2-methylacryl-β-cyclodextrin acting as reductant and stabilizer without using any additional reducing agent is reported. The reduction was carried out in aqueous solution under pH neutral conditions

• at room temperature. The results of dynamic light scattering analysis and transmission electron microscopy show adjustable particle sizes from 30–100 nm, due to variation of silver nitrate concentration, the polymeric reducing and stabilisation agent concentration or reaction time. The spherical

• -β-cyclodextrin [20] as reduction and steric stabilization agent without any additional reduction agent or energy source. Silver nitrate has been reduced under pH neutral conditions at room temperature. In this green one pot synthesis neither commonly used toxic reduction agent nor additional energy