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Search for "cell culture" in Full Text gives 182 result(s) in Beilstein Journal of Nanotechnology.
Evaluating the toxicity of TiO2-based nanoparticles to Chinese hamster ovary cells and Escherichia coli: a complementary experimental and computational approach
Beilstein J. Nanotechnol. 2017, 8, 2171–2180, doi:10.3762/bjnano.8.216
- , as described previously [53]. Toxicity to Chinese hamster ovary (CHO-K1) cells Nanoparticles were ground for 5 min using a mortar and pestle, suspended to a concentration of 1 mg/mL in complete cell culture medium with 0.1% pluronic F68 (cytotoxicity assay) or TSB (MIC determination) and sonicated in
- . Because Au nanoparticles absorb light in the visible region, the plates were centrifuged to avoid interference with the assay. At the next step, 100 µL of medium from each cell culture was transferred to a 96-well plate and the absorbance at 450 nm was measured. Cell viability was calculated as means of
Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics
Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188
- treated with only cell culture medium were used as a control. The cell viability percentage was above 80%, showing that CNOs exhibited moderate toxicity to the cells at the tested concentrations (Figure 6). The observed high viability of the HeLa cells treated with CNOs demonstrated their suitability for
- . Cell culture HeLa cells (obtained from a human cervix carcinoma) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies) supplemented with 10% fetal bovine serum (FBS) (Life Technologies), 100 IU/mL penicillin and 100 μg mL−1 (Life Technologies) in humidified atmosphere at 37 °C
Synthesis and functionalization of NaGdF4:Yb,Er@NaGdF4 core–shell nanoparticles for possible application as multimodal contrast agents
Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183
- (Figure 3c), indicating that the Tween 80 was successfully coated onto the UCNPs. Additionally, dynamic light scattering (DLS) was employed to measure the hydrodynamic diameter of Tween-coated UCNPs in the cell culture medium as well as their surface zeta potential. The measured mean hydrodynamic diameter
Luminescent supramolecular hydrogels from a tripeptide and nitrogen-doped carbon nanodots
Beilstein J. Nanotechnol. 2017, 8, 1553–1562, doi:10.3762/bjnano.8.157
- materials. Peptide self-assembled hydrogels are inherently biocompatible and biodegradable and thus are promising biomaterials for cell culture, regenerative medicine, tissue engineering, and drug delivery applications [22]. The identification of self-assembling peptides that are as short as possible is
Calcium fluoride based multifunctional nanoparticles for multimodal imaging
Beilstein J. Nanotechnol. 2017, 8, 1484–1493, doi:10.3762/bjnano.8.148
- cytotoxicity of the NPs was tested by a cell culture based viability assay. Results and Discussion Synthesis and characterization of the multifunctional nanoparticles The synthesis of the CaF2:(Tb3+,Gd3+) NPs was carried out in analogy to the reported wet-chemical procedure that is based on a co-precipitation
- without appropriate surface modification have a disposition to agglomerate and sediment subsequently under physiological conditions because of their pH value and salt content [39][40][41]. One crucial requirement for the application of NPs in cell-culture experiments or animal testing is the stabilization
- in physiological media. In contrast to an electrostatically stabilization of the NPs, for example by capping the CaF2 NPs surface with citrate groups [28], we ensure the stability of the NPs in serum-containing cell-culture media in an electrosterical way. To this end, a polymer consisting of a
Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery
Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145
- their drug encapsulation, release profile and anticancer activity on MCF-7 human breast cancer cell line in particular. Safety and apoptotic efficacy of blank and PCX-loaded cationic or anionic amphiphilic CD nanoparticles were evaluated with cell culture studies against a series of healthy and cancer
- therefore be suggested that the surface charge of nanoparticle is directly effective on the drug release profile. Cell culture studies In order to determine the safety of blank amphiphilic CD nanoparticles and the anticancer efficacy of PCX-loaded amphiphilic CD nanoparticles, L929 mouse fibroblast cells
- concentration dependent and that they are also non-hemolytic [24][45]. Therefore, these nanoparticles may be safe on healthy cells as drug carrying systems. To optimize the concentration of CD nanoparticles for cell culture studies, the inhibitory concentration 50 (IC50) value of PCX was calculated on MCF-7
Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment
Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144
- Background: Brain tumors are the most common tumors among adolescents. Although some chemotherapeutics are known to be effective against brain tumors based on cell culture studies, the same effect is not observed in clinical trials. For this reason, the development of drug delivery systems is important to
- particles, and the drug release rate from the nanoparticles was slowed down to 48 h by dispersing the nanoparticles in a hydroxypropyl cellulose film. Cell culture studies revealed that docetaxel-loaded nanoparticles cause higher cytotoxicity compared to the free docetaxel solution in DMSO. Conclusion
- recurrence during the first 2 days. Cell culture studies Cytotoxicity assay for blank nanoparticles Mouse fibroblast cell lines L929 (recommended by the USP for the cytotoxicity evaluation of polymeric systems) were used to determine the cytotoxicity of blank nanoparticles with MTT assay. According to MTT
Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers
Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141
- , Figure S1). Based upon previous experience, we limited exposure times to 6 hours in order to reduce the risk of released free dye and fragmentation of the nanoparticles, stemming from partial solubility in cell culture medium, which could confuse uptake and transport studies [42]. Nanoparticle size and
- injected before each measurement to calibrate the instrument, followed by 100 µL of the undiluted particle dispersion. Cell culture and exposure to nanoparticles Caco-2 epithelial cells (supplied by European Collection of Authenticated Cell Cultures) were cultured in complete Dulbecco's Modified Eagle
Micro- and nano-surface structures based on vapor-deposited polymers
Beilstein J. Nanotechnol. 2017, 8, 1366–1374, doi:10.3762/bjnano.8.138
- was generated, and a subsequent cell-culture study showed that cell-signaling adenovirus was correlated along the copolymer gradients [74]. A similar combinatorial approach has also been demonstrated to generate poly(diethylaminoethylacrylate) and poly(dimethylaminomethylstyrene) gradients using an
Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation
Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132
- previously described [27][28]. Cell culture The human PCa cell line DU-145 (HTB-81; ATCC, Rockville, MD, USA) was cultured in Dulbecco’s modified minimum essential medium supplemented with 10% fetal bovine serum, 1% 1 M HEPES buffer and 1% MEM non-essential amino acids (all from Invitrogen, Karlsruhe
- , Germany) at standard conditions (37 °C, humidified atmosphere containing 5% CO2). During treatment 1% streptomycin/penicillin (Invitrogen) was added to the cell culture medium. Cells were cultured in microplates and subsequently treated with the indicated agents. Individual treatments with carbon
- carbon nanomaterials alone for 22 h, afterwards the respective chemotherapeutic was added for another 2 h. Following incubation, the cells were washed twice with PBS and incubated with fresh cell culture medium for another 72 h followed by the conduction of the cellular assays. Cellular viability assay
Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy
Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130
- , but indicate a much higher photostability and brightness. As revealed by dynamic light scattering and ζ-potential measurements, all particle suspensions were stable in water and cell culture medium. In addition, uptake studies on A549 cells were performed, using confocal and stimulated emission
- makes the particles more stable against bleaching. Particle stability in cell culture medium To investigate the behaviour of nanoparticles under experimentally relevant conditions with regard to in vitro or in vivo applications, the particle stability was tested in cell culture media, which contain high
Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets
Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125
- ideal scenario would be a toolbox of commercially available Qdot-Abs that can be consistently used to label any biological structure of interest and not just tubulin and extracellular targets. Methods Cell culture Human cervix epithelioid carcinoma (HeLa, ECACC number 930210a3) cells were cultured in a
Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery
Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123
- induced to differentiate in vitro into adipogenic, osteogenic, chondrogenic and myogenic cells. Moreover, other cell types, such as neurons, glial cells and smooth muscle cells, could be obtained from MSCs under the appropriate cell culture conditions [9][10]. Among the broad variety of investigated NPs
- of 1 × 105 MSCs were first labelled with 16 nM QDs for 6 h in complete medium. After the primary labelling, the cell-culture supernatant was aspirated, the cells were rigorously rinsed and fresh complete or serum-free medium was added. The number of QD-positive cells was assessed using flow cytometry
- pathways. The loss of QD signal over time may possibly be explained by the excretion of QDs from MSCs, which could favour the use of MSCs as drug delivery vectors. These data validate the potential use of skin MSCs as NP delivery vectors for tumour-targeted therapies. Experimental Mesenchymal stem cell
Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification
Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120
- % humidity and 5% carbon dioxide in air. 75 cm2 cell culture flasks (658170; Greiner Bio-One GmbH, Germany) were used for cultivation of the cell lines. Every two or three days the medium was changed until approximately 100% confluence was reached. Cells were detached from the substrate by a 0.05% of trypsin
Silicon microgrooves for contact guidance of human aortic endothelial cells
Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72
- for its application in biotechnology and biomedicine [27][28]. Silicon dioxide is nontoxic and biocompatible, and based on these features it has been proposed as material for drug delivery in cell culture models and for tissue engineering [29]. In addition, silicon offers a flexible surface chemistry
- substrates for 2 days. After cell culture experiments, culture media were removed and cells were washed twice with PBS at 37 °C and afterwards fixed as previously described [31]. Afterwards, adhesion to silicon substrates, morphology and proliferation of HAECs were assessed using SEM (JEOL model JSM-6400
- ), as described further below. Staining on actin and nuclei and fluorescence confocal microscopy HAECs were cultured on the functionalized substrates for 2 and 7 days. After cell culture experiments, culture media were removed and cells were washed twice with PBS at 37 °C and afterwards fixed as
Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles
Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40
- functionalized nanoparticles per cell in the cell culture experiments was calculated accordingly. Cell line culture and imaging Human epithelial cervical cancer cells (HeLa) and human osteosarcoma cells (MG-63) cell lines were cultured in DMEM, supplemented with 10% fetal calf serum (FCS) under 37 °C, 5% CO2 and
- humidified atmosphere and cultivated according to standard cell culture protocols. A primary cell culture of human mesenchymal stem cells (hMSCs) was cultivated using mesenchymal stem cell (MSC) growth medium, supplemented according to the standard cultivation protocol. Approximately 12 h prior to the
- experiments, the cells were trypsinized and seeded in 24-well cell culture plates with 1·105 cells per well in 0.5 mL cell medium. The incubation with nanoparticles was carried out by adding a 50 µL aliquot of the particle dispersion to each well (i.e., the nanoparticle dispersion was diluted 1:11). The cells
Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling
Beilstein J. Nanotechnol. 2016, 7, 1905–1917, doi:10.3762/bjnano.7.182
- applications of O-dots for alive/fixed cell staining and labelling of layer-by-layer polyelectrolyte microcapsules were evaluated. Keywords: cell culture; citric acid; layer-by-layer (LbL)-microcapsules; luminescence; organic dots (O-dots); staining; toxicity; Introduction Luminescent nanosized semiconductor
Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring
Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179
- durable package compatible with cell culture. The LTCC-packaged sensor chip was integrated with a printed circuit board, data acquisition device, and measurement-controlling software. The packaged sensor chip functioned well in the presence of cell medium and cells, with output voltages depending on the
- due to mechanical disturbance caused by the procedure. Biocompatibility of the LTCC package The earlier reported [23] biocompatibility of the LTCC package to cell culture was also confirmed here for the new LTCC material by growing BEAS2B cells on a dummy chip and on the surrounding LTCC. The
- integration of microfluidics into the package was demonstrated. Normal cell morphology on packaged dummy chips demonstrated the feasibility of using the LTCC package for cell culture; no cytotoxicity was observed. Furthermore, it was possible to obtain sensor measurements in real time. The capacitance varied
Chitosan-based nanoparticles for improved anticancer efficacy and bioavailability of mifepristone
Beilstein J. Nanotechnol. 2016, 7, 1861–1870, doi:10.3762/bjnano.7.178
- cell culture A549 human lung cancer cells, human epithelial carcinoma Hela cells, human endometrial carcinoma RL95-2 cells, and human hepatocellular liver carcinoma HepG2 cells were purchased from Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). A549 and Hela was grown in
Nano- and microstructured materials for in vitro studies of the physiology of vascular cells
Beilstein J. Nanotechnol. 2016, 7, 1620–1641, doi:10.3762/bjnano.7.155
- techniques and the selection of materials for micro/nanostructured substrates as well as common geometries please refer to Table 1. 1.1 Fabrication methods In order to create tailored cell culture substrates with surface topographies established methods such as photolithography, electron- and focused-ion
- methods [55] process polymeric material and are also applied to fabricate cell culture substrates. However, the substrates resulting from these fabrication methods are in most cases (irregularly) porous, foam-like 3D structures rather than (symmetrical) surface-patterned substrates. 1.3 Nanofabrication
- as combined polymeric materials, they frequently lack a clearly defined architecture, and they have a variable chemical composition and often complex mechanical properties. These disadvantages and difficulties in using natural polymers for the fabrication of cell culture substrates strongly motivated
Influence of hydrothermal synthesis parameters on the properties of hydroxyapatite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1586–1601, doi:10.3762/bjnano.7.153
- cells and osteosarcoma cells) [10]. The cell culture experiment confirmed that in comparison to conventional HAp, cytophilicity of the nanophase mineral improved with nano-HAp. In addition, an increased viability and spread of stem cells was observed for nano-HAP, in particular for the smallest 20 nm
On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121
- , threshold setting and a final watershed algorithm. Particle detection was restricted between an upper and lower cut off in particle radius and to a circularity greater than 0.7. This way only separated particles were considered for measurement. Cell culture For TEM experiments, all different cell lines were
- suspension was added to the cell culture medium, yielding the final concentration for exposure. For transmission electron microscopy and for cytotoxicity assays, cells were incubated with SiNPs for 10 min to 24 h at 37 °C and 5% CO2 before further processing. To check if the observed particle engulfment is
- York City, U.S.A.) in triplicates and incubated for 20 h. After particle load and incubation, cells were centrifuged for 10 min at 600g. Then, 10 µL of cell culture supernatant was transferred into a fresh 96-well plate, mixed with 100 µL of the LDH reaction mix and incubated for 60 min at room
High antiviral effect of TiO2·PL–DNA nanocomposites targeted to conservative regions of (−)RNA and (+)RNA of influenza A virus in cell culture
Beilstein J. Nanotechnol. 2016, 7, 1166–1173, doi:10.3762/bjnano.7.108
- ). It was demonstrated that these nanocomposites exhibited a low toxicity and very high activity against IAV in the cell culture [18][19]. Only one site in the IAV 5 segment was used in our previous works. In this work, we examined the antiviral activity of DNA fragments in the proposed TiO2·PL–DNA
- inhibit the reproduction of IAV in cell culture. TiO2 nanoparticles (of ≈5 nm in diameter) are known to penetrate through cell membrane [23]. It was clearly demonstrated in our previous work [17][18] that they are good vehicles to transport DNA fragments into cells. There are literature data that show
Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles
Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84
- of both types of the nanoparticles occurred via macropinocytosis as confirmed by TEM. Recently, PLL-γ-Fe2O3 agglomeration properties were studied in biological cell culture media with or without common serum protein, which showed the increase of size and negative ζ-potential in comparison to
- , Great Britain). The agglomeration properties and the surface charge properties of PLL-γ-Fe2O3 nanoparticles in biological cell culture medium with and without addition of common serum protein were previously described [26]. The crystal structure of both types of nanoparticles was investigated using the
- . All experiments were carried out in accordance with the EU Directive 2010/63/EU on the protection of animals used for scientific purposes. Neural stem cell culture Neural stem cells (NSCs) were isolated from pregnant female mice as previously described [28][29]. Briefly, at gestation day 14.5, embryos
Novel roles for well-known players: from tobacco mosaic virus pests to enzymatically active assemblies
Beilstein J. Nanotechnol. 2016, 7, 613–629, doi:10.3762/bjnano.7.54
- , energy conversion, plasmonics or magnetic resonance imaging [82][92][100][114][115][116][117][118][119][120][121], and as carrier rods for effector peptides for distinct purposes from affinity binding, intravital targeting up to cell-culture supports [105][111][117][122][123][124], or as antigens for
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