Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• monitored by dynamic light scattering and the dose deposited onto the cell surface was determined by a modelling approach. The murine macrophage cell line J774A.1 was then used to study uptake and intracellular fate by means of laser scanning microscopy (LSM), environmental scanning electron microscopy

• ). Depolarized dynamic light scattering measurements show that all particles analysed remained stable in both single and co-exposure experiments over 24 h in complete cell culture media (not shown). See Figures S1–S13 (Supporting Information File 1) for a full description of particle synthesis and

• ., AuNPs with 2% DY505 fluorophore, and FeOxNPs with 2% DY615 fluorophore integrated into the polymer shell were synthesized as previously described [44][45]. For both NPs the hydrodynamic radius (dh) was measured by dynamic light scattering (DLS) and a corresponding core diameter (dc = 2·rc) was extracted

Synthesis and characterization of noble metal–titania core–shell nanostructures with tunable shell thickness

• Bartosz Bartosewicz,
• Marta Michalska-Domańska,
• Malwina Liszewska,
• Dariusz Zasada and
• Bartłomiej J. Jankiewicz

Beilstein J. Nanotechnol. 2017, 8, 2083–2093, doi:10.3762/bjnano.8.208

• prevent it. In addition, the confirmation of the shell formation (in almost all reported cases) has been based only on electron microscopy (mainly TEM) images and UV–vis spectroscopy, with a few exceptions of the additional use of either static or dynamic light scattering (SLS or DLS) [21][27]. Here, we

High-stress study of bioinspired multifunctional PEDOT:PSS/nanoclay nanocomposites using AFM, SEM and numerical simulation

• Alfredo J. Diaz,
• Hanaul Noh,
• Tobias Meier and
• Santiago D. Solares

Beilstein J. Nanotechnol. 2017, 8, 2069–2082, doi:10.3762/bjnano.8.207

• Cloisite Na+ and that irregularities must be prevented to reduce detrimental light scattering within the film [11]. It has also been previously shown that the addition of nanoclay helps in the formation of ordered phases in the polymer [25][31], which may lead in PEDOT:PSS nanocomposites to morphologies

Growth and characterization of textured well-faceted ZnO on planar Si(100), planar Si(111), and textured Si(100) substrates for solar cell applications

• Chin-Yi Tsai,
• Jyong-Di Lai,
• Shih-Wei Feng,
• Chien-Jung Huang,
• Chien-Hsun Chen,
• Fann-Wei Yang,
• Hsiang-Chen Wang and
• Li-Wei Tu

Beilstein J. Nanotechnol. 2017, 8, 1939–1945, doi:10.3762/bjnano.8.194

• regarding the subject of granular ZnO thin films grown on crystalline silicon substrates. It is known that due to the formation of nanometer ZnO grains, granular ZnO could increase the light-scattering capability (i.e., the haze) of the film and thus function as a light-trapping structure by enhancing the

Fabrication of carbon nanospheres by the pyrolysis of polyacrylonitrile–poly(methyl methacrylate) core–shell composite nanoparticles

• Dafu Wei,
• Youwei Zhang and
• Jinping Fu

Beilstein J. Nanotechnol. 2017, 8, 1897–1908, doi:10.3762/bjnano.8.190

• concentrations of MB, respectively, V (L) is the volume of MB aqueous solution, and m (g) is the weight of carbon nanospheres. Characterization The z-average hydrodynamic diameter of the various nanoparticles was determined by dynamic light scattering (DLS) using a Malvern Zeta-sizer (DTS1060, Malvern, UK). The

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• laser which can alter the measurements due to background signal. Dynamic light scattering (DLS) was carried out to determine the hydrodynamic radius of the dispersed CNOs (Table 2, Figure 5). DLS experiments were performed on CNOs samples dispersed in 0.01 M PBS to partially mimic the biological

• concentrations of 20, 10 and 5 μg mL−1. Dynamic light scattering and zeta potential measurements DLS measurements were performed on the Malvern Nano-ZS instrument operating in backscattering (173°) mode and analyzed with the Zetasizer software, with automatic selection of the optimal detector position and number

• emission maximum (λem). Effective hydrodynamic diameter (0.01 M PBS) obtained from dynamic light scattering (DLS) measurements and zeta potential (phosphate buffer) of oxi-CNOs and fluo-CNOs at a concentration of 5 µg mL−1 in PBS. Supporting Information Supporting Information File 82: Additional

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• Mueller polarimetric images in clinical settings will be sensitive not to precancerous epithelial transformations but rather to stromal modifications induced by CIN [111]. Hence, the decrease of depolarization power in CIN zones of thick tissue can be attributed to both a decrease of light scattering and

• tissue. This technique makes use of low-coherence interferometry with a near-infrared light source to create two-dimensional images of tissue cross-sections by exploring elastic light scattering from internal tissue microstructures [117]. OCT provides depth-resolved images, where the contrast results

Self-assembly of chiral fluorescent nanoparticles based on water-soluble L-tryptophan derivatives of p-tert-butylthiacalix[4]arene

• Pavel L. Padnya,
• Irina A. Khripunova,
• Olga A. Mostovaya,
• Timur A. Mukhametzyanov,
• Vladimir G. Evtugyn,
• Vyacheslav V. Vorobev,
• Yuri N. Osin and
• Ivan I. Stoikov

Beilstein J. Nanotechnol. 2017, 8, 1825–1835, doi:10.3762/bjnano.8.184

• stable, positively charged aggregates of 86–134 nm in diameter in water at a concentration of 1 × 10−4 M as confirmed by dynamic light scattering, scanning electron microscopy and transmission electron microscopy. It was established that these aggregates are fluorescently active and chiral. A distinctive

• structure of L-tryptophan, the resulting compounds 8–11 are water soluble. The structure and composition of the synthesized compounds 8–11 were determined by 1H and 13C NMR, IR spectroscopy, ESI mass spectrometry and elemental analysis. The method of dynamic light scattering (DLS) is widely used to study

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• (Figure 3c), indicating that the Tween 80 was successfully coated onto the UCNPs. Additionally, dynamic light scattering (DLS) was employed to measure the hydrodynamic diameter of Tween-coated UCNPs in the cell culture medium as well as their surface zeta potential. The measured mean hydrodynamic diameter

• equations: where ni and Di are the number and diameter of the particle, respectively. Dynamic light scattering (DLS) was used to determine the hydrodynamic particle diameter and zeta potential. These experiments were performed with Brookhaven ZetaPALS zeta potential analyzer (Brookhaven Instruments, USA

• potential. The results representing hydrodynamic size distribution of UCNPs and their zeta potential that were measured using dynamic light scattering method (DLS). Acknowledgements This work was supported by the grant “Study of optical properties and biocompatibility of Na(Gd/Y)F4 nanoparticles” (No.MIP

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• diamond nanoparticles The size of the ND particles and their zeta potential was determined by dynamic light scattering (DLS) measurements in water at 25 °C using a Nano-ZetaSizer (Malvern, UK) equipped with an He-Ne laser. A separate disposable folded capillary cell was used for each set of ND

A nanocomplex of C₆₀ fullerene with cisplatin: design, characterization and toxicity

• Svitlana Prylutska,
• Svitlana Politenkova,
• Kateryna Afanasieva,
• Volodymyr Korolovych,
• Kateryna Bogutska,
• Andriy Sivolob,
• Larysa Skivka,
• Maxim Evstigneev,
• Viktor Kostjukov,
• Yuriy Prylutskyy and
• Uwe Ritter

Beilstein J. Nanotechnol. 2017, 8, 1494–1501, doi:10.3762/bjnano.8.149

• ., 25, 98693 Ilmenau, Germany 10.3762/bjnano.8.149 Abstract The self-organization of C60 fullerene and cisplatin in aqueous solution was investigated using the computer simulation, dynamic light scattering and atomic force microscopy techniques. The results evidence the complexation between the two

• lymphocytes and reduces the fraction of necrotic cells. Keywords: atomic force microscopy; C60 fullerene; cisplatin; comet assay; computer simulation; dynamic light scattering; flow cytometry; human lymphocytes; toxicity in vitro; Introduction The water-soluble inorganic bi-valent platinum derivative

• of the hydrodynamic size distribution was performed by dynamic light scattering (DLS) on a Zetasizer Nano ZS (Malvern Ins. Ltd) with upload of multiple narrow modes (high resolution) at room temperature. The instrument is equipped with a He–Ne gas laser (max. output power 5 mW) operating at a

Calcium fluoride based multifunctional nanoparticles for multimodal imaging

• Marion Straßer,
• Joachim H. X. Schrauth,
• Sofia Dembski,
• Daniel Haddad,
• Bernd Ahrens,
• Stefan Schweizer,
• Bastian Christ,
• Alevtina Cubukova,
• Marco Metzger,
• Heike Walles,
• Peter M. Jakob and
• Gerhard Sextl

Beilstein J. Nanotechnol. 2017, 8, 1484–1493, doi:10.3762/bjnano.8.148

• because of the low solubility of NH4F in an ethanol solution and therefore the particle growth is slower [26]. The inset in Figure 1 shows the TEM micrograph of the CaF2:(Tb3+,Gd3+) NPs. The NPs possess a spherical shape with an average diameter of 5–10 nm. The results of the dynamic light scattering (DLS

• decreases within 2 h because of light scattering on NP agglomerates. Light-microscopy images of dispersions of stabilized and non-stabilized CaF2:(Tb3+,Gd3+) NPs in FCS-containing cell-culture medium are given in Figure S3 (Supporting Information File 1). Finally, the viability of human dermal fibroblasts

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• mL ultrapure water using the conditions previously given. Mean particle size distribution and surface charge The mean particle diameter (nm), PDI and zeta potential (mV) of amphiphilic CD nanoparticles were determined by dynamic light scattering (DLS) (NanoZS, Malvern Instruments, UK). All

Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

• Cem Varan and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

• was removed from the surface of the PET film to obtain the final product. Nanoparticle characterization Particle size distribution and surface charge analysis: the mean particle diameter and polydispersity index of the nanoparticles were determined by dynamic light scattering (DLS) technique using a

Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• , with no signs of agglomeration, though naturally serum protein adsorption increases the sizes. Table 1 also shows auxiliary dispersion characterisation by dynamic light scattering (DLS), leading to the same conclusions. Nanoparticle dispersions were prepared with and without FBS protein in order to

• serum (FBS) in Hank's balanced salt solution (HBSS) buffer (with 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES), pH 7.4) at 25 °C, using a Malvern Zetasizer Nano ZS90 (Worcestershire, UK) in order to measure the hydrodynamic diameter by dynamic light scattering (DLS). The 150 nm

• . Nanoparticle dispersion (100 µg/mL) in water, serum free HBSS and HBSS supplemented with 10% foetal bovine serum (FBS) were characterised by differential centrifugal sedimentation (DCS) and dynamic light scattering (DLS) as described in the Experimental section. The table shows the apparent diameter and peak

Miniemulsion copolymerization of (meth)acrylates in the presence of functionalized multiwalled carbon nanotubes for reinforced coating applications

• Bertha T. Pérez-Martínez,
• Lorena Farías-Cepeda,
• Víctor M. Ovando-Medina,
• José M. Asua,
• Lucero Rosales-Marines and
• Radmila Tomovska

Beilstein J. Nanotechnol. 2017, 8, 1328–1337, doi:10.3762/bjnano.8.134

• by quasielastic light scattering (QLS) with a ZEN1600 apparatus (Malvern Instruments). The samples were prepared by diluting one drop of latex in deionized water. The reported diameters are the average of two subsequent measurements. It should be noted that the content of MWCNTs after dilution is

Synthesis of [Fe(L_eq)(L_ax)]_n coordination polymer nanoparticles using blockcopolymer micelles

• Christoph Göbel,
• Ottokar Klimm,
• Florian Puchtler,
• Sabine Rosenfeldt,
• Stephan Förster and
• Birgit Weber

Beilstein J. Nanotechnol. 2017, 8, 1318–1327, doi:10.3762/bjnano.8.133

• dynamic light scattering (DLS) in solution, transmission electron microscopy (TEM) and powder X-ray diffraction (PXRD) in the solid. The hydrodynamic diameter of the polymeric micelles loaded with the CP measured by DLS is constant within the error of the measurement throughout all measured samples with

• were corrected for the diamagnetism of the sample holder, the polymer matrix (measured values) and the ligand (tabulated Pascal constants). Dynamic light scattering: The samples were measured using a Malvern Instruments Zetasizer Nano ZS90 in glass cuvettes from Carl Roth GmbH + Co. KG at 25 °C. One

• particles. b) Autocorrelation function from dynamic light scattering of 3e in THF (43 wt %) with size histogram. c) Size histogram of the core from the TEM picture given in panel a). Characterisation of the magnetic properties of 1d and 3e Top: Mössbauer spectra of 1d (left) and 3e (right). Bottom: Magnetic

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

• Isabella Tavernaro,
• Christian Cavelius,
• Henrike Peuschel and
• Annette Kraegeloh

Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130

• , but indicate a much higher photostability and brightness. As revealed by dynamic light scattering and ζ-potential measurements, all particle suspensions were stable in water and cell culture medium. In addition, uptake studies on A549 cells were performed, using confocal and stimulated emission

• (5% size distribution; FD15_Atto647N) or 25 nm (4%; FD25_Atto647N) could be obtained (Figure 1). Hydrodynamic diameters determined by dynamic light scattering (DLS) were in accordance with diameters obtained from SEM or TEM image analysis (Table 1). These results show that no agglomerates of the

• . See Supporting Information File 1 for the synthesis of pure silica nanoparticles and different dye embedded nanoparticles. Characterisation To determine the particle diameters, the morphology and the agglomeration state, the samples were analysed by dynamic light scattering measurements (DLS

Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification

• Mohamed Hassoun,
• Iwan W.Schie,
• Tatiana Tolstik,
• Sarmiza E. Stanca,
• Christoph Krafft and
• Juergen Popp

Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120

• cells with subsequent microscopic assessment by pathologists. Complementary tools are immunocytochemistry, which uses fluorescence-labeled antibodies against cellular antigens, and flow cytometry, which combines several detection channels based on light scattering, absorption and fluorescence with

• microfluidic flow systems. Raman spectroscopy has been proposed as promising technique for cell characterization and cell identification because of its high chemical specificity under label-free and non-destructive conditions [1][2]. Raman spectroscopy is based on inelastic light scattering from molecular

Growth, structure and stability of sputter-deposited MoS₂ thin films

• Reinhard Kaindl,
• Bernhard C. Bayer,
• Roland Resel,
• Thomas Müller,
• Viera Skakalova,
• Gerlinde Habler,
• Rainer Abart,
• Alexey S. Cherevan,
• Dominik Eder,
• Maxime Blatter,
• Fabian Fischer,
• Jannik C. Meyer,
• Dmitry K. Polyushkin and
• Wolfgang Waldhauser

Beilstein J. Nanotechnol. 2017, 8, 1115–1126, doi:10.3762/bjnano.8.113

• , electron beam and light scattering, scanning and spectroscopic methods as well as electrical device characterization. We find that room-temperature-deposited MoS2 films are amorphous, of smooth surface morphology and easily degraded upon moderate laser-induced annealing in ambient conditions. In contrast

Study of the correlation between sensing performance and surface morphology of inkjet-printed aqueous graphene-based chemiresistors for NO₂ detection

• F. Villani,
• C. Schiattarella,
• T. Polichetti,
• R. Di Capua,
• F. Loffredo,
• B. Alfano,
• M. L. Miglietta,
• E. Massera,
• L. Verdoliva and
• G. Di Francia

Beilstein J. Nanotechnol. 2017, 8, 1023–1031, doi:10.3762/bjnano.8.103

• LPE graphene dispersion has been investigated by dynamic light scattering (DLS) to determine the size distribution profile of the suspended graphene flakes. An almost monomodal distribution characterized by a low polydispersity has been detected, so indicating the homogeneity of the sample. The

• of five measurements, have been evaluated by the Owen, Wendt, Rabel and Kaelble (OWRK) method [21]. Both disperse and polar components of SE have been estimated. Dynamic light scattering (DLS) analysis has been performed at T = 25 °C by means of a Zetasizer Nano (Malvern Instruments). DLS

Selective detection of Mg²⁺ ions via enhanced fluorescence emission using Au–DNA nanocomposites

• Tanushree Basu,
• Khyati Rana,
• Niranjan Das and
• Bonamali Pal

Beilstein J. Nanotechnol. 2017, 8, 762–771, doi:10.3762/bjnano.8.79

• (λmax varied from 524 to 637 nm) [18][19][20][21]. The sample AuNS-4 exhibited a maximum shift (λmax ≈ 15 nm) relative to other nanospheres as these larger sized particles exhibit more light scattering. The AuNS-1 sample resulted in a shift from 524 to 538 nm upon addition of DNA, while DNA does not

• AuNPs. The TEM images of AuNSs and AuNRs are shown in Figure 3, which reveal the formation of Au–DNA NCs [29]. These AuNSs were found to be separated from each other due to the CTAB coating on their surface, which renders them to have a positive charge [30]. The dynamic light scattering (DLS) results

• nanocomposites. Dynamic light scattering particle size distribution of bare and DNA modified (a,b) AuNS-1, (c,d) AuNRs and (e) their comparative average diameter data. AFM images of (a,b) AuNS 1-DNA nanocomposites and (c,d) AuNR–DNA nanocomposites showing height and amplitude mode. 3D AFM images of Au–DNA

Dispersion of single-wall carbon nanotubes with supramolecular Congo red – properties of the complexes and mechanism of the interaction

• Anna Jagusiak,
• Barbara Piekarska,
• Tomasz Pańczyk,
• Małgorzata Jemioła-Rzemińska,
• Elżbieta Bielańska,
• Barbara Stopa,
• Grzegorz Zemanek,
• Janina Rybarska,
• Irena Roterman and
• Leszek Konieczny

Beilstein J. Nanotechnol. 2017, 8, 636–648, doi:10.3762/bjnano.8.68

• . The aim of the study was to explain the mechanism of this interaction. The interaction of CR and carbon nanotubes was studied using spectral analysis of the SWNT–CR complex, dynamic light scattering (DLS), differential scanning calorimetry (DSC) and microscopic methods: atomic force microscopy (AFM

• complexes at increased temperature allows the aggregation of nanotubes, followed by the reassociation of CR with the surface of SWNT bundles (thus the supernatant is colourless). Dynamic light scattering analyses Dynamic light scattering (DLS) applied to SWNT–CR complexes analysis does not provide precise

• buffers). Each set of data was analysed using NanoAnalyze software supplied by TA Instruments. Dynamic light scattering (DLS) The interaction of CR with carbon nanotubes was studied using the DynaPro NanoStar (Wyatt Technology). The measurements were done after 3 min incubation at 25 °C within the

α-((4-Cyanobenzoyl)oxy)-ω-methyl poly(ethylene glycol): a new stabilizer for silver nanoparticles

• Jana Lutze,
• Miguel A. Bañares,
• Marcos Pita,
• Andrea Haase,
• Andreas Luch and
• Andreas Taubert

Beilstein J. Nanotechnol. 2017, 8, 627–635, doi:10.3762/bjnano.8.67

• synthesis, that is, no dilution or other sample processing was performed prior to Raman analysis. NMR spectroscopy. 1H NMR spectra were recorded on a Bruker Avance 300 MHz or 500 MHz spectrometer in D2O with TMS as an internal standard. Light scattering and zeta potential. The hydrodynamic diameter Rh and

• distribution. CBAmPEG-modified SNPs (CBAmPEG@SNPs) Citrate-coated SNPs (citrate@SNP) [46] were reacted with CBAmPEG at room temperature to obtain SNPs coated with CBAmPEG (CBAmPEG@SNP). The particles were characterized with Raman spectroscopy, dynamic light scattering (DLS), and transmission electron

• dispersion at pH 7 via dynamic light scattering (DLS) yields a hydrodynamic radius Rh of around 60 nm after dialysis; often the samples have a bimodal size distribution (Table 1). Figure 2C shows Raman spectra of a dispersion of CBAmPEG@SNP and of an aqueous solution of the pure polymer CBAmPEG. The intense

Investigation of the photocatalytic efficiency of tantalum alkoxy carboxylate-derived Ta₂O₅ nanoparticles in rhodamine B removal

• Subia Ambreen,
• Mohammad Danish,
• Narendra D. Pandey and
• Ashutosh Pandey

Beilstein J. Nanotechnol. 2017, 8, 604–613, doi:10.3762/bjnano.8.65

• nanoparticles efficiently removed rhodamine B under UV light irradiation. Keywords: alkoxy carboxylates; band gap; dynamic light scattering (DLS); rhodamine B; scanning electron microscopy (SEM); tantalum(V) oxide (Ta2O5) nanoparticles; TEM; X-ray diffraction (XRD); Introduction Nowadays, the purification of

• nanoparticles samples are found to be in the range of 120–170 nm. The oxide nanoparticles are moderately evenly dispersed in all cases. DLS measurements To study the properties of nanoparticles in dispersion dynamic light scattering (DLS) experiments were carried out. Stable dispersions of Ta2O5 nanoparticles