Graphene oxide–chloroquine conjugate induces DNA damage in A549 lung cancer cells through autophagy modulation

• Braham Dutt Arya,
• Sandeep Mittal,
• Prachi Joshi,
• Alok Kumar Pandey,
• Jaime E. Ramirez-Vick,
• Govind Gupta and
• Surinder P. Singh

Beilstein J. Nanotechnol. 2025, 16, 316–332, doi:10.3762/bjnano.16.24

• single/double strand DNA breaks, DNA crosslinks, base damages, and apoptotic nuclei [57]. Figure 7 reveals the DNA damage analysis in GO–Chl-exposed A549 cells. In Figure 7a, the digital images of DNA comets obtained by florescence microscopy indicates that GO–Chl exposure induces a strong genotoxic

• and animal models [23][64]. To elucidate the role of autophagy in DNA damage in GO–Chl-exposed A549 cells, we performed a set of experiments focusing on autophagosomes. Figure 8a reveals the florescence-microscopy-based MDC staining for labeling autophagic vacuoles in GO–Chl-exposed A549 cells. The

Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension

• Khosro Adibkia,
• Ali Ehsani,
• Asma Jodaei,
• Ezzatollah Fathi,
• Raheleh Farahzadi and
• Mohammad Barzegar-Jalali

Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62

• incubated with CD31, CD34, CD73, and CD44 antibodies for 30 min at 4 °C. Next, a FACS instrument was used to quantify the florescence intensity of cells. Also, the multilineage differentiation potential was assessed. BM-MSCs were cultured in adipogenic and osteogenic induction medium for 21 days. At the end

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• , which stains apoptotic cells or nuclei and yields blue florescence when bound to dsDNA in the cell nucleus [42] with excitation/emission wavelength at 352/455 nm. Rhodamine B, with an excitation/emission wavelength of 543/565 nm, provides a red fluorescence emission. A drastic decrease in the Hoechst

Electrospun one-dimensional nanostructures: a new horizon for gas sensing materials

• Muhammad Imran,
• Nunzio Motta and
• Mahnaz Shafiei

Beilstein J. Nanotechnol. 2018, 9, 2128–2170, doi:10.3762/bjnano.9.202

Strategy to discover full-length amyloid-beta peptide ligands using high-efficiency microarray technology

• Clelia Galati,
• Natalia Spinella,
• Lucio Renna,
• Danilo Milardi,
• Francesco Attanasio,
• Michele Francesco Maria Sciacca and
• Corrado Bongiorno

Beilstein J. Nanotechnol. 2017, 8, 2446–2453, doi:10.3762/bjnano.8.243

• well as the low sample consumption and the high potential for miniaturization, suggests that the proposed combination of peptide microarrays and highly efficient slides would be a very effective technology for molecule profiling in AD drug discovery. Keywords: Alzheimer's disease; florescence; high

• the different behavior of the two peptides, the florescence images were acquired with different laser and gain conditions. For incubated KLVFF spots, a lower laser power than for Semax was used. Otherwise, saturation of the detector would have been reached, which is not useful for quantitative

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• to remove adherent protein and then analysed with light and florescence microscopy (Figure 2). The only case where a dissolved protein was taken up by cells was for HTRA1 with MG-63 cells. In all other cases, no significant uptake of the dissolved proteins was observed. To deliver the proteins HTRA1