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Search for "trypsin" in Full Text gives 69 result(s) in Beilstein Journal of Nanotechnology.
Graphene oxide–chloroquine conjugate induces DNA damage in A549 lung cancer cells through autophagy modulation
Beilstein J. Nanotechnol. 2025, 16, 316–332, doi:10.3762/bjnano.16.24
- ). Phosphate-buffered saline (Ca+2, Mg+2 free; PBS; Cat. No. 14200166), Dulbecco’s modified Eagle’s medium: nutrient mixture F-12 (Ham) (1:1) powder (DMEM F-12; Cat. No. 11320033), trypsin–EDTA (Cat. No. 25200056), fetal bovine serum (FBS; Cat. No. A5256701), antibiotic and antimycotic solution (100X; 10,000 U
- to 25 μg/mL of GO–Chl for 24 h and washed with 1× PBS to remove any GO–Chl nanoconjugates not uptaken by the cells. Cells were then harvested using trypsin–EDTA, washed with 1× PBS followed by fixation with 2.5% glutaraldehyde for 4 h. Then, fixed cells were washed using 0.1 M of sodium cacodylate
- with 1× PBS, harvested using 0.25% trypsin, and centrifuged at 1000 rpm for 10 min to remove excess GO–Chl nanoconjugates. Furthermore, cells were resuspended in 100 μL of 1× PBS and incubated with the PI dye (stock: 1 mg/mL; working solution: 2 μL/100 μL) for 10 min at room temperature in the dark
Radiosensitizing properties of dual-functionalized carbon nanostructures loaded with temozolomide
Beilstein J. Nanotechnol. 2025, 16, 229–251, doi:10.3762/bjnano.16.18
Mechanistic insights into endosomal escape by sodium oleate-modified liposomes
Beilstein J. Nanotechnol. 2024, 15, 1667–1685, doi:10.3762/bjnano.15.131
- No. 15250061), and trypsin-EDTA solution (Catalog No. 25200056) were supplied by Gibco, Thermo Fisher Scientific (Waltham, MA, USA). Fluorescent dyes LysoSensor Green DND-189 (Catalog No. L7535) and Hoechst 33342 (Catalog No. H3570) were also procured from Thermo Fisher Scientific. The stearylated
A biomimetic approach towards a universal slippery liquid infused surface coating
Beilstein J. Nanotechnol. 2024, 15, 1376–1389, doi:10.3762/bjnano.15.111
- was added to each well of a 96-well plate blocked with BSA. After incubation for 60 min, 90 μL of protein solution was removed and added to a new plate containing 5 μL 4.7mM soybean trypsin inhibitor (Sigma). 5 μL of chromogenic substrate S-2302 (Chromogenix) was added to each well and the absorbance
Dual-functionalized architecture enables stable and tumor cell-specific SiO2NPs in complex biological fluids
Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100
- performed with dithiothreitol and iodoacetamide, respectively, followed by in-gel trypsin digestion. The peptides were desalted on a C18 Stage Tips column and an aliquot containing 2 µg of each sample was analyzed using an LTQ Orbitrap Velos (Thermo Fisher Scientific) coupled to a nanoflow EASY-nLC (Proxeon
- , released in 2022) using the Andromeda search engine. Carbamidomethylation of cysteines (+57.02 Da) was set as fixed modifications, and protein N-terminal acetylation (+42.01 Da) and methionine oxidation (+15.99 Da) were set as variable modifications. Trypsin/P was set as the proteolytic enzyme, with a
- the cells were washed 3 times with PBS and removed from culture plates by trypsinization. Next, the cells were centrifuged to remove trypsin and subsequently washed with PBS. Following that, 4% paraformaldehyde was added for 20 min. Finally, the cells were centrifuged again, washed with PBS, and
Therapeutic effect of F127-folate@PLGA/CHL/IR780 nanoparticles on folate receptor-expressing cancer cells
Beilstein J. Nanotechnol. 2024, 15, 954–964, doi:10.3762/bjnano.15.78
- ) (MT35010CV), antibiotic (15-240-062), and trypsin (25-200-056) were purchased from Gibco, Fisher Scientific. Conjugation of pluronic F127 and folic acid The conjugation of folic acid to Pluronic F127 was done as described in our previous study [34]. Folic acid (0.4 mmol) was activated by 1,1
Electrospun polysuccinimide scaffolds containing different salts as potential wound dressing material
Beilstein J. Nanotechnol. 2024, 15, 781–796, doi:10.3762/bjnano.15.65
- , USA); trypsin/EDTA solution (Sigma-Aldrich, USA); cell proliferation reagent WST-1 (Roche, Switzerland); and ultra-purified water (Zineer Power I Water Purification System). All reagents were used without any further purification. Polysuccinimide synthesis The PSI was produced by thermal
- % CO2 in a humidified atmosphere, and subcultivated at a subconfluent state by using trypsin/EDTA (0.05%). The medium used for the MG-63 cell line was composed of MEM supplemented with 10% of fetal bovine serum, 1% of non-essential amino acids, 2 mM of ʟ-glutamine, 100 IU/mL of penicillin, and 100 µg/mL
Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent
Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46
- , USA). CytoPainter Phalloidin-iFluor 488 was purchased from Abcam plc. (UK). Endothelial Growth Medium-2 (EGM-2) was obtained from Lonza (Swiss). Roswell Park Memorial Institute 1640 (RPMI), penicillin–streptomycin, glutamine, sodium pyruvate, and trypsin/ethylenediamine tetraacetic acid were purchased
Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics
Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17
- Sigma-Aldrich. Dulbecco's Modified Eagle Medium (DMEM) (11965092), fetal bovine serum (FBS) (MT35010CV), antibiotic (15-240-062), and trypsin (25-200-056) were purchased from Gibco, Fisher Scientific. All other solvents and reagents were of chemical grade. Synthesis of iron oxide nanoparticles Iron
Development and characterization of potential larvicidal nanoemulsions against Aedes aegypti
Beilstein J. Nanotechnol. 2024, 15, 104–114, doi:10.3762/bjnano.15.10
- 10% bovine fetal serum and 100 μg/mL of penicillin G/streptomycin. Maintained at 37 °C with 5% CO2, the cells were grown until they reached a subconfluent density. To detach the cells, a 5 min trypsin treatment with TrypLE™ Express at 37 °C was performed, followed by inactivation using 0.3 mg/mL
- trypsin inhibitor. The cells were then centrifuged at 500g for 5 min, resuspended in DMEM, and placed overnight in 96-well microplates (200 μL/well, 1 × 106 cells/mL) at 37 °C with 5% CO2. After incubation, nanoemulsions, free terpenes, and surfactant solutions were administered at concentrations from 0.1
Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency
Beilstein J. Nanotechnol. 2024, 15, 71–82, doi:10.3762/bjnano.15.7
- scaffolds The cell culture medium was prepared by mixing 150 mL of Dulbecco’s modified eagle medium (DMEM) with 600 μL of 0.5 mg/mL trypsin in a Schott bottle. MA-104 cells were distributed into the wells of a 96-well plate in DMEM supplemented with 10% fetal bovine serum. The BBR NPs/PLA nanofiber scaffold
Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies
Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66
- 50 µM) or the free drug were added. After 72 h of treatment, the cells were harvested with a trypsin/EDTA solution and processed for flow cytometry analysis using a BD Biosciences FACSCANTO II Flow Cytometer (Franklin Lakes, NJ, USA). Propidium iodide (Sigma, St. Louis, USA) was added to the cell
In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124
- solution made of trypsin-EDTA was added and incubated at 37 °C for 3 min in a humidified incubator with 5% CO2 to produce a cell suspension. Trypsinized cells were reseeded in fresh medium at 105 cells/mL and incubated at 37 °C in a 5% CO2 humidified incubator. All reagents were purchased from Biowest
Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects
Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123
- quantity of 0.25% trypsin/0.02% EDTA. The cells were transferred in a 75 cm2 flask and cultured in mesenchymal stem cell expansion medium under the same conditions, changing the cell culture medium every second day, until 70–90% cellular confluence. After detachment, the cells were split and cultured in
Gelatin nanoparticles with tunable mechanical properties: effect of crosslinking time and loading
Beilstein J. Nanotechnol. 2022, 13, 778–787, doi:10.3762/bjnano.13.68
- degradation of the particles using trypsin for 6 h. The results revealed a crosslinking-dependent loading, with increased FITC-dextran loading for a longer crosslinking. The initial incorporation of FITC-dextran had a significant influence on Young’s moduli. The correlation between Young’s moduli and the
- loading of FITC-dextran was determined by dissolving the gelatin network using enzymatic degradation. The fluorescence intensity of the resulting solution was measured [18]. Therefore, 5 mg of freeze-dried GNPs were dispersed in 5 mL PBS containing 2.5 mg trypsin at room temperature. After 6 h of
Effects of substrate stiffness on the viscoelasticity and migration of prostate cancer cells examined by atomic force microscopy
Beilstein J. Nanotechnol. 2022, 13, 560–569, doi:10.3762/bjnano.13.47
- solution, respectively. In order to allow for the acclimatisation of cells to different substrates, cells were inoculated and cultured on hydrogel substrates with stiffness values of 3, 19, and 35 kPa. Next, cells were digested with 0.25% trypsin after 48 h and continued in the hydrogel on the same
- substrate. This was done for three repeated passages. Cells were digested with trypsin and counted before other experiments were carried out [44]. Preparation of polyacrylamide (PAA) gel substrates The preparation of polyacrylamide hydrogels with different stiffness referred to the experimental method of
Stimuli-responsive polypeptide nanogels for trypsin inhibition
Beilstein J. Nanotechnol. 2022, 13, 538–548, doi:10.3762/bjnano.13.45
- inflammatory mediator trypsin. Two types of nanogels were prepared from linear synthetic polypeptides based on biocompatible and biodegradable poly[N5-(2-hydroxyethyl)-ʟ-glutamine-ran-N5-propargyl-ʟ-glutamine-ran-N5-(6-aminohexyl)-ʟ-glutamine]-ran-N5-[2-(4-hydroxyphenyl)ethyl)-ʟ-glutamine] (PHEG-Tyr) or
- different loading capacities for AAT with the maximum (20%) achieved with Nα-Lys-NG nanogel. In both cases, the nanogel depots demonstrated a burst release of AAT during the first 6 h, which could be favorable for quick inhibition of trypsin. A consequent pilot in vitro inhibition study revealed that both
- PHEG-Tyr and Nα-Lys-NG nanogels loaded with AAT successfully inhibited the enzymatic activity of trypsin. Furthermore, the inhibitory efficiency of the AAT-loaded nanogels was higher than that of only AAT. Interestingly, also non-loaded PHEG-Tyr and Nα-Lys-NG nanogels were shown to effectively inhibit
Ethosomal (−)-epigallocatechin-3-gallate as a novel approach to enhance antioxidant, anti-collagenase and anti-elastase effects
Beilstein J. Nanotechnol. 2022, 13, 491–502, doi:10.3762/bjnano.13.41
- (−)-Epigallocatechin-3-gallate, 3-(4,5-dimethyldiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), SPC, trypsin-EDTA solution, dimethyl sulfoxide (DMSO) for cell culture, and penicillin/streptomycin solution were purchased from Sigma, USA. Carbopol 980 was purchased from the Abdi İbrahim Pharmaceutical, Industry and
Theranostic potential of self-luminescent branched polyethyleneimine-coated superparamagnetic iron oxide nanoparticles
Beilstein J. Nanotechnol. 2022, 13, 82–95, doi:10.3762/bjnano.13.6
- with 10% of FBS and 1% of penicillin solution. The cells were kept at 37 °C under 5% CO2 and were subcultured three times per week with 0.25% trypsin/EDTA. The cells were seeded onto 96-well microtiter plates (Greiner) at a concentration of 5 × 103 cells/well (HeLa and MCF7) and 10 × 103 cells/well
Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles
Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99
- Institute (RPMI-1640, 99.9%) medium, GPPS (2 mM ʟ-glutamine, 1 mM Na pyruvate, 100 U/mL penicillin, 100 µg/mL streptomycin, 98%), Triton X-100 (lab grade), Trizma base, trypsin/EDTA (lab grade), sulforhodamine B (dye content 75%), ethidium bromide (lab grade), sodium dodecyl sulfate (SDS, lab grade), 2',7
- ) with 10% CO2. The cells were harvested using trypsin/EDTA (0.5 mM) for 1 min at room temperature. In vitro cytotoxicity screening of drug-loaded MFe2O4 (M = Fe, Co, Ni, Zn) NPs The cytotoxicity screening of colloidal drug-loaded MFe2O4 (M = Fe, Co, Ni, Zn) NPs was performed by using the SRB assay in
pH-driven enhancement of anti-tubercular drug loading on iron oxide nanoparticles for drug delivery in macrophages
Beilstein J. Nanotechnol. 2021, 12, 1127–1139, doi:10.3762/bjnano.12.84
- /nanoparticle treatment the cells were trypsinized using 1X trypsin-EDTA (HiMedia, India). The cell counts of the trypsinized cells were then determined using the trypan blue assay. Briefly, a fixed volume of animal cell sample was taken and diluted using a 0.4% solution of trypan blue (HiMedia, India). The
Silver nanoparticles induce the cardiomyogenic differentiation of bone marrow derived mesenchymal stem cells via telomere length extension
Beilstein J. Nanotechnol. 2021, 12, 786–797, doi:10.3762/bjnano.12.62
- by PBS and trypsinized with trypsin-EDTA. The 4 × 103 cells were seeded in each well of a 96-well plate with a total volume of 200 μL of cardiomyogenic differentiation medium of different concentrations (0, 1.25, 2.5, and 5 µg/mL) of Ag-NPs and incubated for 14 days at 37 °C in a humidified
PEG/PEI-functionalized single-walled carbon nanotubes as delivery carriers for doxorubicin: synthesis, characterization, and in vitro evaluation
Beilstein J. Nanotechnol. 2020, 11, 1728–1741, doi:10.3762/bjnano.11.155
- were obtained from Beijing Solarbio Science & Technology Co., Ltd (Beijing, China). Trypsin–EDTA solution (0.25% trypsin with 0.02% EDTA) was supplied by Beyotime Biotechnology (Beijing, China). 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) was purchased from Sigma-Aldrich (St
- wells, and the cells were trypsinized with trypsin–EDTA and washed with cold PBS for three times to remove the nanocarriers outside the cells. The cells were then collected and resuspended in 200 μL of PBS by centrifugation. The mean fluorescence intensity (MFI) was determined using FCM (BD Biosciences
Identification of physicochemical properties that modulate nanoparticle aggregation in blood
Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44
- -PAGE) instrument for 10 min before the protein bands were excised from the gel in order to allow the whole corona proteins to migrate into a single gel band. The proteins in the gel pieces were reduced with dithiothreitol, alkylated with iodoacetamide and digested with trypsin (Promega Corporation
- and protein N-terminal acetylation. A maximum of two missed trypsin cleavages were allowed in the database search. The false discovery rate for both peptides and proteins was set at 1%. After that, the ProteinGroup file from Maxquant was processed, filtered and analysed with Perseus software to
Luminescent gold nanoclusters for bioimaging applications
Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42
- electrostatic interaction was ruled out. Instead, the authors assumed that it might be a specific peptide motif of HSA that interacts with the bacterial cell wall. The trypsin digestion of Au-HSA NCs was studied and various fragments were identified using MALDI–MS. To confirm further whether the peptides can
- imaging [91]. Recently Duan et al. reported the synthesis of NIR-luminescent AuNCs capped with N-acetyl-ʟ-cysteine (NAC-CS) for long-time imaging [92]. The Au-NAC-CS NCs were insensitive to hydrogen peroxide and trypsin in contrast to Au NCs coated with BSA or other proteins, allowing for extended imaging
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