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Search for "imaging" in Full Text gives 237 result(s) in Beilstein Journal of Organic Chemistry. Showing first 200.
Development of a fluorogenic small substrate for dipeptidyl peptidase-4
Beilstein J. Org. Chem. 2017, 13, 2690–2697, doi:10.3762/bjoc.13.267
- the reaction medium. The results suggest that 1 could be used as fluorophore in OFF–ON-type fluorogenic probes. Keywords: dipeptidyl peptidase-4; fluorogenic substrate; fluorometry; small fluorescent molecule; Introduction Fluorescent organic probes have become indispensable for bio-imaging or
- molecular imaging to visualize the presence of species such as particular enzymes and biologically important inorganic ions. In biomedicine, these probes are used for the detection of a wide range of significant biomarkers and are essential for the diagnosis of critical diseases [1][2][3]. Selective and
Electrophilic trifluoromethylselenolation of terminal alkynes with Se-(trifluoromethyl) 4-methylbenzenesulfonoselenoate
Beilstein J. Org. Chem. 2017, 13, 2626–2630, doi:10.3762/bjoc.13.260
- Clement Ghiazza Anis Tlili Thierry Billard Institute of Chemistry and Biochemistry, Univ Lyon, Université Lyon 1, CNRS, 43 Bd du 11 novembre 1918, F-69622 Villeurbanne, France CERMEP-In vivo Imaging, Groupement Hospitalier Est, 59 Bd Pinel, F-69003 Lyon, France 10.3762/bjoc.13.260 Abstract Herein
Regioselective decarboxylative addition of malonic acid and its mono(thio)esters to 4-trifluoromethylpyrimidin-2(1H)-ones
Beilstein J. Org. Chem. 2017, 13, 2617–2625, doi:10.3762/bjoc.13.259
- Organofluorine compounds now play an essential role in the development of new materials for solar cells [1][2][3], radiotracers for PET imaging [4], agrochemicals [5][6], sensitive chemical probes for 19F nuclear magnetic resonance investigation of biological experiments [7][8], and are most widely used in the
Pyrene–nucleobase conjugates: synthesis, oligonucleotide binding and confocal bioimaging studies
Beilstein J. Org. Chem. 2017, 13, 2521–2534, doi:10.3762/bjoc.13.249
- investigated towards application as fluorescent cell imaging bioprobes so far. Furthermore, self-assembly studies of pyrene–nucleobases on oligonucleotide templates have not been reported as well. The work presented herein addresses these two problems. Accordingly, in this contribution we report on the
- that, due to significant phototoxic activity, compounds 2 and 3 were not compatible with live cell imaging. On the other hand, accumulation of 4 and 5 was detectable with confocal microscopy, following 15 min incubation of live human cancer HeLa cells with 200 nM of each compound. Both 4 and 5 produced
- results substantiate further studies on pyrene–nucleobase conjugates as nucleic acid fluorescent probes and as cell imaging agents. Experimental General All preparations were carried out using standard Schlenk techniques. Chromatographic separations were carried out using silica gel 60 (Merck, 230–400
Hydrolysis, polarity, and conformational impact of C-terminal partially fluorinated ethyl esters in peptide models
Beilstein J. Org. Chem. 2017, 13, 2442–2457, doi:10.3762/bjoc.13.241
- development of peptide-based contrast agents for 19F imaging [22]. In polypeptides, the incorporation of fluorine can significantly alter the properties of the native molecule. The hydrophobicity [23][24], conformational equilibria [25], and the thermodynamic [26][27][28][29] and kinetic [30][31] folding
Asymmetric synthesis of propargylamines as amino acid surrogates in peptidomimetics
Beilstein J. Org. Chem. 2017, 13, 2428–2441, doi:10.3762/bjoc.13.240
- proteases [7], cruzain 20 [8][9], caspases [10] and peptidyl aminopeptidases [11]. These protease inhibitors show potential for the treatment of Chagas disease [2][9], Huntington’s disease [10], malaria [11], autoimmune diseases [6] and the imaging of tumor associated macrophages [2]. Whereas the carboxylic
Exploring mechanochemistry to turn organic bio-relevant molecules into metal-organic frameworks: a short review
Beilstein J. Org. Chem. 2017, 13, 2416–2427, doi:10.3762/bjoc.13.239
- ], luminescence [67], non-linear optics [68] and magnetism [69], as well as contrast agents for magnetic resonance imaging (MRI) [70] and as drug carriers in systems for controlled drug delivery and release [64][71][72][73][74][75][76][77][78][79][80]. Also under development are new systems with potential use in
- anticancer drugs have also shown that these have potential to be used in fluorescence imaging. The number of reports on MOFs synthesized by mechanochemistry [8][28][50][99][100][101] has been increasing and some in situ studies on the mechanosynthesis of MOFs and coordination polymers are already being
Structure–property relationships and third-order nonlinearities in diketopyrrolopyrrole based D–π–A–π–D molecules
Beilstein J. Org. Chem. 2017, 13, 2374–2384, doi:10.3762/bjoc.13.235
- investigated for DPP derivatives. The most common applications of organic molecules with THG activity are directed towards all-optical signal processing and optical imaging [30][31]. Hence, as a continuation of our research activity on incorporating heteroaromatic moieties in push–pull molecules [9][10], we
One-pot syntheses of blue-luminescent 4-aryl-1H-benzo[f]isoindole-1,3(2H)-diones by T3P® activation of 3-arylpropiolic acids
Beilstein J. Org. Chem. 2017, 13, 2340–2351, doi:10.3762/bjoc.13.231
- pattern of the naphthalene scaffold. Even the smallest polarity change in the solvent system effects their absorption and emission behavior [34][40]. Very recent investigations on naphthaleneimide derivatives revealed enormous phosphorescence lifetimes that are particularly interesting for imaging
Synthesis and application of trifluoroethoxy-substituted phthalocyanines and subphthalocyanines
Beilstein J. Org. Chem. 2017, 13, 2273–2296, doi:10.3762/bjoc.13.224
- –vis spectrum, do not aggregate in solution. These dyes are expected to be developed into medical imaging probes. Phthalocyanine is expected to be used not only as a biological imaging agent but also in cancer treatment. Photodynamic therapy (PDT) is a laser cancer therapy that introduces organic dyes
Phosphonic acid: preparation and applications
Beilstein J. Org. Chem. 2017, 13, 2186–2213, doi:10.3762/bjoc.13.219
- bone targeting, for the design of supramolecular or hybrid materials, for the functionalization of surfaces, for analytical purposes, for medical imaging or as phosphoantigen. These applications are covering a large panel of research fields including chemistry, biology and physics thus making the
- develop bone targeting which was assessed for therapy [96] and imaging [97][98]. Finally, the phosphonic acid group was also employed to immobilize organic or organometallic molecules on the surface of metal oxide [99][100] (Al2O3 [101], TiO2 [102][103], SnO2 [104], Fe3O4 [105], ZnO [106]), CdSe quantum
- dots [107] and used as anchoring group for dye-sensitized solar cells [108][109][110] or for the immobilization of organocatalyst [111][112]. Phosphonic acid was also used for coating superparamagnetic iron oxide (e.g., magnetite) assessed as contrast agent in magnetic resonance imaging [113] or to
Superstructures with cyclodextrins: Chemistry and applications IV
Beilstein J. Org. Chem. 2017, 13, 2157–2159, doi:10.3762/bjoc.13.215
- magnetic resonance imaging (MRI) [22]. Well-defined fluorescent polyrotaxanes with alternating, threaded cucurbit[6]uril and CD rings were assembled by Fraser Stoddart’s group via an alkyne–azide click reaction exploiting supramolecular catalysis [23][24]. The concurrent radical copolymerization of 1,3
Direct catalytic arylation of heteroarenes with meso-bromophenyl-substituted porphyrins
Beilstein J. Org. Chem. 2017, 13, 1524–1532, doi:10.3762/bjoc.13.152
- fields of modern organic chemistry due to their unique structure and electronic properties. These molecules have already proved to be powerful tools in phototherapy, radioimmunotherapy and imaging [1], for cancer treatment [2], and actually sophisticated theranostic porphyrin-based nanodevices have been
- application of such medicaments are antitumor remedies and non-invasive diagnostic imaging agents for PET, SPECT and MRI methods [5]. The combination of porphyrin and heterocyclic moieties in one molecule is perspective for the design of new compounds with potential powerful pharmaceutical properties. Up to
BODIPY-based fluorescent liposomes with sesquiterpene lactone trilobolide
Beilstein J. Org. Chem. 2017, 13, 1316–1324, doi:10.3762/bjoc.13.128
- . Based on this approach, multimodal agents delivered using a vehicle containing a drug capable of both imaging and curing were developed [4]. A meaningful information about biomolecule/drug localization and action can be gained employing fluorescence imaging, since it provides non-invasiveness
- of globular structures, that prepared liposomes were uniform in shape (high variability in shape or inhomogeneous peak structure would extensively increase the roughness value) and the cover over the surface was also homogeneous. Live-cell imaging of construct 6 and its liposomal formulation The
Glycoscience@Synchrotron: Synchrotron radiation applied to structural glycoscience
Beilstein J. Org. Chem. 2017, 13, 1145–1167, doi:10.3762/bjoc.13.114
- proportional to Z4). This phenomenon produces the contrast that is used in X-ray imaging techniques. The synchrotron light spectrum is polychromatic, with a spectral bandwidth that depends on the type and configuration of the sources. Many experiments require a narrower bandwidth, or a monochromatic beam, and
Glyco-gold nanoparticles: synthesis and applications
Beilstein J. Org. Chem. 2017, 13, 1008–1021, doi:10.3762/bjoc.13.100
- resulting biological processes [3][4]. The increasing ability to manipulate material at the nanosize allowed the development of many glyco nanoparticles helpful in a wide range of applications, from the drug delivery to the imaging [5][6][7]. Among the large number of nanomaterials, gold is one of the main
- demonstrated that GLUT1-targeting GAuNPs are well compatible with blood and do not significantly interact with ovine red blood cells [93]. In the last years, numerous compounds containing Gd3+ have been developed and used as magnetic resonance imaging (MRI) contrast agents. Recently, gold based nanosystems
Expression, purification and structural analysis of functional GABA transporter 1 using the baculovirus expression system
Beilstein J. Org. Chem. 2017, 13, 874–882, doi:10.3762/bjoc.13.88
- % phosphotungstic acid or 2% ammonium molybdate in the presence of 0.1% trehalose) was added, blotted again and air-dried. Imaging was performed using a Tecnai F20 FEG (FEI Company, Oregon) at an accelerating voltage of 160 kV under low-dose conditions. Micrographs were recorded according to the low-dose protocol
- and -stage (Model 626). Samples were constantly cooled by LN2 during imaging to maintain a sample temperature of T = 93 K. Imaging was performed at an accelerating voltage of 160 kV with a defocus value of 600 nm, which corresponds to the first zero of the CTF at 13 Å (Cs = 2.0 mm). Micrographs were
Fluorescent carbon dots from mono- and polysaccharides: synthesis, properties and applications
Beilstein J. Org. Chem. 2017, 13, 675–693, doi:10.3762/bjoc.13.67
- traditional semiconductor quantum dots (QDs). Particularly in the areas of live imaging and drug delivery, due to their water solubility, low toxicity and photo- and chemical stability. Carbohydrates are readily available chiral biomolecules in nature which offer an attractive and cheap starting material from
- fluorophores, in addition to bright visible emission, have become particularly popular for their versatility as non-isotopic detection labels which are amenable to live cell imaging and immunoassay applications [7]. In particular, cadmium-based QDs (e.g., CdS, CdSe, CdSe/ZnS) are commonly used for in vitro
- fluorescent nanomaterials combined with their myriad of applications, has led to CDs being hailed as the potential non-toxic successors to traditional semiconductor QDs, particularly in the areas of live imaging and drug delivery. Synthetic approaches to access CDs can be classified into two broad categories
Fast and efficient synthesis of microporous polymer nanomembranes via light-induced click reaction
Beilstein J. Org. Chem. 2017, 13, 558–563, doi:10.3762/bjoc.13.54
- conductivity. All specimen were imaged under high-vacuum conditions (1.0 Torr), using an acceleration voltage of 20 keV. Atomic force microscopy (AFM): AFM-imaging was performed using an Asylum Research Atomic Force Microscope, MFP-3D BIO. The AFM was operated at 25 °C in an isolated chamber in alternating
Novel β-cyclodextrin–eosin conjugates
Beilstein J. Org. Chem. 2017, 13, 543–551, doi:10.3762/bjoc.13.52
- preservation of the photophysical properties of the dye. In fact, this molecular hybrid exhibits satisfactory fluorescence and 1O2 photogeneration quantum yields making it a suitable candidate for biomedical research studies in the field of imaging and PDT applications. The possibility to exploit the CD cavity
Investigation of the action of poly(ADP-ribose)-synthesising enzymes on NAD+ analogues
Beilstein J. Org. Chem. 2017, 13, 495–501, doi:10.3762/bjoc.13.49
- labelling approaches comprising different reporter-modified NAD+ building blocks have stimulated and enriched proteomic studies and imaging applications of ADP-ribosylation processes. Herein, we compare the substrate scope and applicability of different NAD+ analogues for the investigation of the polymer
- activity [15][16][17][18], the identification of PARylation sites and targets [15][19][20] and the real-time imaging [21] of PARylation processes. In this report, we systematically compare the substrate scope of the four poly(ADP-ribose)-synthesising enzymes ARTD1, ARTD2, ARTD5 and ARTD6. For this purpose
A postsynthetically 2’-“clickable” uridine with arabino configuration and its application for fluorescent labeling and imaging of DNA
Beilstein J. Org. Chem. 2017, 13, 127–137, doi:10.3762/bjoc.13.16
- yield. Especially mixed combinations, that means donor dyes with arabino-configured anchor with acceptor dyes with ribo-configured anchor, and vice versa, showed significantly improved fluorescence properties. Those were successfully applied for fluorescent imaging of DNA after transport into living
- traffic lights” [17][19][25] that are energy transfer-based nucleic acid probes that can be used in molecular beacons [26], especially for vesicular microRNA imaging in living cancer cells [27], and for siRNA transport imaging [28]. Donor and acceptor dyes are combined in an interstrand and diagonal
- -green contrast of 86). In order to test the functionality of the respective dyes as FRET pairs in DNA duplexes for imaging in cells, four representative duplexes, DNA2aD1–DNA3rD5, DNA2rD1–DNA3aD8, DNA2aD2–DNA3aD8 and DNA2rD4–DNA3aD8, were tested in HeLa cells. 5 × 104 HeLa cells were transiently
Versatile synthesis of end-reactive polyrotaxanes applicable to fabrication of supramolecular biomaterials
Beilstein J. Org. Chem. 2016, 12, 2883–2892, doi:10.3762/bjoc.12.287
- modified with dibenzylcyclooctyne (DBCO)-conjugated fluorescent molecules via a copper-free click reaction; this fluorescently labeled PRX was utilized for intracellular fluorescence imaging. The method of synthesizing end-reactive PRXs described herein is simple and versatile for the design of diverse
- -catalyzed click reactions are performed. Additionally, fluorescence labeling of the PRX and intracellular fluorescence imaging were performed as an example application. Results and Discussion Synthesis of azide-terminated PRXs using bis(2-amino-3-phenylpropyl) poly(ethylene glycol) and 4-substituted benzoic
- fluorescence imaging Terminal reactive groups in the PRXs can be utilized in various biomaterials applications, such as the fabrication of cross-linked materials (e.g., hydrogels) [29], direct surface immobilization onto alkyne-immobilized surfaces [30], and the modification of other functional molecules for
Biochemical and structural characterisation of the second oxidative crosslinking step during the biosynthesis of the glycopeptide antibiotic A47934
Beilstein J. Org. Chem. 2016, 12, 2849–2864, doi:10.3762/bjoc.12.284
- Biochemistry and Molecular Biology and ARC Centre of Excellence in Advanced Molecular Imaging, Monash University, Clayton, Victoria 3800, Australia 10.3762/bjoc.12.284 Abstract The chemical complexity and biological activity of the glycopeptide antibiotics (GPAs) stems from their unique crosslinked structure
Chemical probes for competitive profiling of the quorum sensing signal synthase PqsD of Pseudomonas aeruginosa
Beilstein J. Org. Chem. 2016, 12, 2784–2792, doi:10.3762/bjoc.12.277
- selectively targeting the active site of an enzyme and a reporter group that allows in-gel imaging and/or affinity enrichment of target enzymes [22]. We thus synthesized a small library of chemical probes with electrophilic α-chloroacetamide, α,β-unsaturated amide, and α,β-unsaturated ketone moieties as
- and excess reporter tag were removed by SDS-polyacrylamide gel electrophoreses (SDS-PAGE), and labelling of the protein was visualized by fluorescence imaging. Consistency of protein levels was checked by coomassie staining (Supporting Information File 1, Figures S2 and S3). While the α,β-unsaturated
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