Comparative study on 3D morphologies of delignified, single tracheids and fibers of five wood species

• Helen Gorges,
• Felicitas von Usslar,
• Cordt Zollfrank,
• Silja Flenner,
• Imke Greving,
• Martin Müller,
• Clemens F. Schaber,
• Chuchu Li and
• Stanislav N. Gorb

Beilstein J. Nanotechnol. 2026, 17, 239–250, doi:10.3762/bjnano.17.16

• largely absent from scientific literature. Although previous studies have examined wood anatomy using various imaging techniques, such as X-ray micro-computed tomography in addition to scanning and transmission electron microscopy [22][23][24][25][26][27], high-resolution 3D reconstructions of single

• , Pdet = 6.5 µm pixel size, 2048 × 2048 pixel, 16 bit image depth) with a 10 µm Gadox scintillator was used as the detector. For high-contrast and low-dose imaging, holotomography was applied as the phase contrast technique. Here, a gold Fresnel zone plate with a diameter of 300 µm was used [30]. By

• arrangements of single fibers and tracheids with unprecedented 3D details in spatial distribution compared to traditional methods such as SEM, which, however, show more details of the pit structures [40][41][42]. Unlike those conventional imaging methods, nano-CT 3D models provide a comprehensive view of pit

Gold nanoparticle-decorated reduced graphene oxide as a highly effective catalyst for the selective α,β-dehydrogenation of N-alkyl-4-piperidones

• Brenda Flore Kenyim,
• Mihir Tzalis,
• Marilyn Kaul,
• Robert Oestreich,
• Aysenur Limon,
• Chancellin Pecheu Nkepdep and
• Christoph Janiak

Beilstein J. Nanotechnol. 2026, 17, 218–238, doi:10.3762/bjnano.17.15

• , the successful AuNP deposition on the carbon materials was validated through TEM imaging of Au-Cit/AC (Figure 5a), Au-Cit/rGO (Figure 5b), and Au-Cit/CB (Figure 5c). Upon deposition, the average AuNP particle sizes slightly grew to 13 nm within a range of 10–16 nm determined by TEM images and the

• , explaining the observed 100% selectivity. Because the Au-SiW9/rGO composite exhibited the most promising activity among the tested catalysts, its structural robustness was examined through leaching experiments and TEM imaging after successive reaction cycles. To confirm the solid-state (heterogeneous) nature

Time of flight secondary ion mass spectrometry imaging of contaminant species in chemical vapour deposited graphene on copper

• Barry Brennan,
• Vlad-Petru Veigang-Radulescu,
• Philipp Braeuninger-Weimer,
• Stephan Hofmann and
• Andrew J. Pollard

Beilstein J. Nanotechnol. 2026, 17, 200–213, doi:10.3762/bjnano.17.13

• resolving power, and imaging capability of ToF-SIMS allow us to explore variations in the chemical species present on the graphene surface, as well as in three dimensions under the graphene. In this way, we can observe the impact that variations in the chemical composition of the copper foil have on the

• characterisation techniques with the ability to distinguish different chemical species present, combined with surface sensitivity and suitably high spatial resolution. We here focus on time-of-flight secondary ion mass spectrometry (ToF-SIMS) imaging of Cu-catalysed graphene CVD samples. ToF-SIMS offers high

• . For 3D imaging, each depth profile was acquired by cyclically analysing a 150 µm × 150 µm area (with a pixel density of 128 × 128) from the centre of a 400 µm × 400 µm sputtered region during depth profiling, to mitigate crater edge effects on the generated spectra. 10 keV Cs+ ions with an ion current

From shield to spear: Charge-reversible nanocarriers in overcoming cancer therapy barriers

• Madhuri Yeduvaka,
• Pooja Mittal,
• Ameer Boyalakuntla,
• Usman Bee Shaik,
• Himanshu Sharma,
• Thakur Gurjeet Singh,
• Siva Nageswara Rao Gajula and
• Lakshmi Vineela Nalla

Beilstein J. Nanotechnol. 2026, 17, 159–175, doi:10.3762/bjnano.17.10

• -reversal system (CDs-Pt (IV)@PEG-PAH/DMA) for imaging-guided drug delivery, which enhanced cancer therapeutic effects [85]. 4 Preclinical and clinical insights of charge-reversible nanocarriers Preclinical studies have revealed that CRNs significantly improve antitumour performance by enhancing tumour

Influence of surface characteristics on the in vitro stability and cell uptake of nanoliposomes for brain delivery

• Dushko Shalabalija,
• Ljubica Mihailova,
• Nikola Geskovski,
• Andreas Zimmer,
• Otmar Geiss,
• Sabrina Gioria,
• Diletta Scaccabarozzi and
• Marija Glavas Dodov

Beilstein J. Nanotechnol. 2026, 17, 139–158, doi:10.3762/bjnano.17.9

Development and in vitro evaluation of liposomes and immunoliposomes containing 5-fluorouracil and R-phycoerythrin as a potential phototheranostic system for colorectal cancer

• Raissa Rodrigues Camelo,
• Vivianne Cortez Sombra Vandesmet,
• Octavio Vital Baccallini,
• José de Brito Vieira Neto,
• Thais da Silva Moreira,
• Luzia Kalyne Almeida Moreira Leal,
• Claudia Pessoa,
• Daniel Giuliano Cerri,
• Maria Vitória Lopes Badra Bentley,
• Josimar O. Eloy,
• Ivanildo José da Silva Júnior and
• Raquel Petrilli

Beilstein J. Nanotechnol. 2026, 17, 97–121, doi:10.3762/bjnano.17.7

• tumor cells, enhancing the internalization of the system and promoting greater cytotoxic activity compared to conventional therapies [10]. By combining selective cytotoxicity with the potential to label cancer tissue using an imaging probe, EGFR-targeted immunoliposomes represent an integrated approach

Reduced graphene oxide paper electrode for lithium-ion cells – towards optimized thermal reduction

• Agata Pawłowska,
• Magdalena Baran,
• Stefan Marynowicz,
• Aleksandra Izabela Banasiak,
• Adrian Racki,
• Adrian Chlanda,
• Tymoteusz Ciuk,
• Marta Wolczko and
• Andrzej Budziak

Beilstein J. Nanotechnol. 2026, 17, 24–37, doi:10.3762/bjnano.17.3

• and thickness were determined with scanning electron microscopy imaging. This paper also reveals electrical and electrochemical properties of the material. The conductivity of the material obtained at 800 °C reached ≈70 S/cm, and the discharge capacity reached ≈160 mAh/g at 100 mA/g current density

• . SEM imaging The morphological properties of the rGO paper were described based on SEM imaging (Phenom ProX). The thicknesses of the obtained sheets were determined from SEM images of the samples’ cross sections as an averaged value calculated over 50 length readings. TGA TGA Q5000 equipment was

Quantitative estimation of nanoparticle/substrate adhesion by atomic force microscopy

• Aydan Çiçek,
• Markus Kratzer,
• Christian Teichert and
• Christian Mitterer

Beilstein J. Nanotechnol. 2026, 17, 1–14, doi:10.3762/bjnano.17.1

• deposited at a bias voltage of 10 V with a scan angle of 90° and a z-scale of 14 nm. The red, green, and white circles highlight three NP manipulation examples: (a) the initial scan for imaging Cu NPs, (b) the second scan where the NPs start to be pushed, and (c) the third scan showing the removal of NPs

Internal 3D temperature mapping in biological systems using ratiometric light-sheet imaging and lipid-coated upconversion nanothermometers

• Dannareli Barron-Ortiz,
• Enric Pérez-Parets,
• Rubén D. Cadena-Nava,
• Emilio J. Gualda,
• Jacob Licea-Rodríguez,
• Juan Hernández-Cordero,
• Pablo Loza-Álvarez and
• Israel Rocha-Mendoza

Beilstein J. Nanotechnol. 2025, 16, 2306–2316, doi:10.3762/bjnano.16.159

• estimate temperature through ratiometric imaging of two thermally coupled fluorescence bands. This work demonstrates the feasibility of volumetric temperature mapping in internal biological systems using light-sheet fluorescence microscopy and lipid-coated UCNPs as nanothermometry markers. This approach

• enables real-time thermal mapping with both high spatial and temporal resolution at the cellular and subcellular levels. To validate the method, we performed 3D temperature imaging on fixed Caenorhabditis elegans (C. elegans) after UCNP ingestion. The proposed technique represents a cutting-edge method

• for accurate 3D analysis of temperature-driven biological processes. It holds significant potential for applications in living organisms, offering a non-invasive tool to monitor intracellular and organ-specific temperature dynamics. Keywords: C. elegans; 3D imaging; fluorescent intensity ratio; light

Improving magnetic properties of Mn- and Zn-doped core–shell iron oxide nanoparticles by tuning their size

• Dounia Louaguef,
• Ghouti Medjahdi,
• Sébastien Diliberto,
• Klaus M. Seemann,
• Thomas Gries,
• Joelle Bizeau,
• Damien Mertz,
• Eric Gaffet and
• Halima Alem

Beilstein J. Nanotechnol. 2025, 16, 2285–2295, doi:10.3762/bjnano.16.157

• unique magnetic properties, small size, and biocompatibility, which enable them to be used in a wide range of biomedical applications. These applications include magnetic resonance imaging, magnetic separation, targeted drug delivery, and hyperthermia [1][2]. Magnetic hyperthermia has been extensively

Visualizing nanostructures in supramolecular hydrogels: a correlative study using confocal and cryogenic scanning electron microscopy

• Shaun M. Smith,
• Ferdinando Malagreca,
• Jacqueline Hicks,
• Giuseppe Mantovani,
• David B. Amabilino,
• Christopher Parmenter and
• Lluïsa Pérez-García

Beilstein J. Nanotechnol. 2025, 16, 2274–2284, doi:10.3762/bjnano.16.156

• visualization of the native fibrous network. These results demonstrate the complementary nature of CLSM and cryo-SEM and highlight the value of cryo-SEM as a very useful tool for imaging soft nanomaterials with low fluorescence or limited optical contrast. Keywords: anion binding; colloid; fluorophore

• forms of microscopy have naturally been undertaken, as comprehensive characterization of such systems requires the application of multiple imaging methods. Within the realm of hydrogels, it is typical to see comparative fluorescence and electron microscopy imaging being used to characterize a given

• material. The application of confocal microscopy, stimulated emission depletion (STED) microscopy, and conventional transmission electron microscopy (TEM) to a supramolecular peptide material has been shown to allow for sub-diffraction resolution imaging of fibre morphology in situ without covalent

Chiral plasmonic nanostructures fabricated with circularly polarized light

• Tian Qiao and
• Ming Lee Tang

Beilstein J. Nanotechnol. 2025, 16, 2245–2264, doi:10.3762/bjnano.16.154

Optical bio/chemical sensors for vitamin B₁₂ analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks

• Seyed Mohammad Taghi Gharibzahedi and
• Zeynep Altintas

Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153

Ultrathin water layers on mannosylated gold nanoparticles

• Maiara A. Iriarte Alonso,
• Jorge H. Melillo,
• Silvina Cerveny,
• Yujin Tong and
• Alexander M. Bittner

Beilstein J. Nanotechnol. 2025, 16, 2183–2198, doi:10.3762/bjnano.16.151

• good contrast [25]. The STEM images in dark-field mode (imaging scattered electrons) show the gold cores as bright structures, whereas the thin organic layers are darker. The carbon grid yields practically no scattering and appears essentially black, such that both the core and shell of the particles

• sensing, imaging, or drug delivery systems. The success of these platforms stems from their dispersion in water, stability, and biocompatibility in fully hydrated states, as well as in biological fluids. Our investigation shows a novel approach to these particles by testing the hydration properties under

• for ppp polarization (all beams polarized normal to the surface) and 2 min for ssp (laser beams polarized parallel to the surface, sum frequency emission and up-conversion polarized perpendicular and infrared parallel to the plane of incidence). Electron microscopy imaging SEM and STEM images were

Microplastic pollution in Himalayan lakes: assessment, risks, and sustainable remediation strategies

• Sameeksha Rawat,
• S. M. Tauseef and
• Madhuben Sharma

Beilstein J. Nanotechnol. 2025, 16, 2144–2167, doi:10.3762/bjnano.16.148

• than SEM. It is particularly useful in morphological examination, size grading, and rapid particle identification. The combination of optical microscopes and modern digital imaging techniques allows for automatic particle classification and counting, which greatly decreases the extent of human

Rapid synthesis of highly monodisperse AgSbS₂ nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense

• Funda Ulusu,
• Adem Sarilmaz,
• Yakup Ulusu,
• Faruk Ozel and
• Mahmut Kus

Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145

• , these materials have also been used in various imaging applications for the detection of cancer cells. Ag2Te and Ag2S nanocrystals (NCs) were used in cancer imaging studies by Nieves and colleagues. In this study, computed tomography contrasts changes of NCs injected into mice were examined at 2 and 24

• findings were consistent across various imaging modalities. Analyzing the HRTEM image in Figure 2c revealed a lattice d-spacing of 3.214 Å, corresponding precisely to the (111) crystallographic planes. The SAED patterns in Figure 2d exhibited distinct, discontinuous diffraction rings with sharp points

Toward clinical translation of carbon nanomaterials in anticancer drug delivery: the need for standardisation

• Michał Bartkowski,
• Francesco Calzaferri and
• Silvia Giordani

Beilstein J. Nanotechnol. 2025, 16, 2092–2104, doi:10.3762/bjnano.16.144

• energy sectors (Figure 1). NMs have seen use as antimicrobial agents [1], catalysts [2], bioimaging agents [3][4][5][6], magnetic particle imaging agents [7], nanofluids [8], antiviral agents [9], photothermal convertors [10], and in environmental remediation [11]. Topically, the biomedical applications

Multifrequency AFM integrating PeakForce tapping and higher eigenmodes for heterogeneous surface characterization

• Yanping Wei,
• Jiafeng Shen,
• Yirong Yao,
• Xuke Li,
• Ming Li and
• Peiling Ke

Beilstein J. Nanotechnol. 2025, 16, 2077–2085, doi:10.3762/bjnano.16.142

• ) technique that synergistically integrates PeakForce tapping mode with higher eigenmode vibrations to achieve simultaneous high-resolution topographical imaging and to access additional contrast channels for distinguishing material regions or compositions. Unlike conventional multimodal AFM, our method

• signals can be correlated with specific interaction types, such as conservative and dissipative processes [10][11][12]. Despite these advances, critical limitations persist. The inherent nonlinearity of tip–sample dynamics in tapping and multimodal AFM often introduces imaging artifacts. Typical examples

• traditional dynamic modes, PFT eliminates the need for intricate cantilever tuning by real-time regulation of the interaction force at each scan point. This innovation not only simplifies operation but also enables high-resolution topographic imaging with exceptional stability, even in challenging

Stereodiscrimination of guests in chiral organosilica aerogels studied by ESR spectroscopy

• Sebastian Polarz,
• Yasar Krysiak,
• Martin Wessig and
• Florian Kuhlmann

Beilstein J. Nanotechnol. 2025, 16, 2034–2054, doi:10.3762/bjnano.16.140

• measurements more difficult. There are other valuable methods for investigating transport in porous media such as gas-adsorption methods, X-ray tomography [24][25], neutron imaging techniques [26][27], optical imaging techniques [27][28], or impedance spectroscopy [29][30]. For all of them, it is difficult to

• ), quantitative evaluation of the spectra allowed for investigating various confinement effects. Our focus was on understanding how a particular functionalization of the surfaces in combination with 3CP and the solvent influences transport properties, and novel ESR imaging techniques were used to determine

Beyond the shell: exploring polymer–lipid interfaces in core–shell nanofibers to carry hyaluronic acid and β-caryophyllene

• Aline Tavares da Silva Barreto,
• Francisco Alexandrino-Júnior,
• Bráulio Soares Arcanjo,
• Paulo Henrique de Souza Picciani and
• Kattya Gyselle de Holanda e Silva

Beilstein J. Nanotechnol. 2025, 16, 2015–2033, doi:10.3762/bjnano.16.139

• -sectional imaging, and attenuated total reflectance with Fourier transform infrared (ATR-FTIR) spectroscopy provided compelling evidence for the successful formation of the intended core–shell structure. The resulting nanofibers exhibited surface hydrophobicity, suggesting potential for anti-adhesive

• SEM imaging of the cross-section of the HA+NE2/PLA sample was performed before and after core removal to observe the core–shell structure. For core removal, a portion of the sample was washed multiple times with distilled water, followed by ethanol, and then dried at room temperature. Samples, both

• distribution of the core within nanofibers produced via coaxial electrospinning. A disk scanning unit confocal microscope (Olympus, Japan) was utilized for this analysis. Fluorescein was added to the core solution (1% HA + 2% NE) to enable fluorescence imaging during microscopic observation. Thermogravimetric

The cement of the tube-dwelling polychaete Sabellaria alveolata: a complex composite adhesive material

• Emilie Duthoo,
• Aurélie Lambert,
• Pierre Becker,
• Carla Pugliese,
• Jean-Marc Baele,
• Arnaud Delfairière,
• Matthew J. Harrington and
• Patrick Flammang

Beilstein J. Nanotechnol. 2025, 16, 1998–2014, doi:10.3762/bjnano.16.138

• electron imaging, the anterior parts of a few worms as well as some reconstructed tube fragments were fixed in Bouin’s fluid for 24 h, dehydrated in graded ethanol, dried by the critical-point method, and mounted on aluminium stubs using carbon adhesive tabs. The samples were then coated with gold

• unfixed, air-dried tube fragment that was subsequently broken, SEM imaging and microanalysis of a fractured cement spot showed one NaCl crystal within each of the pores (Figure 2E, Supporting Information File 1, Figure S1). A decalcified cement spot that held two glass beads together was also observed in

Laser ablation in liquids for shape-tailored synthesis of nanomaterials: status and challenges

• Natalie Tarasenka

Beilstein J. Nanotechnol. 2025, 16, 1963–1997, doi:10.3762/bjnano.16.137

• , Figure 2 demonstrates the correlation of the shape of laser-induced plasma generated in the electric field applied directly to a Zn target with NP structure. The plasma imaging (Figure 2b,c) demonstrates clear differences in shape depending on the applied field direction. In case of cathode ablation, the

Mechanical property measurements enabled by short-term Fourier-transform of atomic force microscopy thermal deflection analysis

• Thomas Mathias,
• Roland Bennewitz and
• Philip Egberts

Beilstein J. Nanotechnol. 2025, 16, 1952–1962, doi:10.3762/bjnano.16.136

• ); mechanical property measurements; surface science; Introduction Atomic force microscopy (AFM) has become an indispensable tool for imaging the surface topography on a variety of surfaces [1]. Since the invention of the AFM [2], several other modes of AFM have been developed, including friction force

Evaluating metal-organic precursors for focused ion beam-induced deposition through solid-layer decomposition analysis

• Benedykt R. Jany,
• Katarzyna Madajska,
• Aleksandra Butrymowicz-Kubiak,
• Franciszek Krok and
• Iwona B. Szymańska

Beilstein J. Nanotechnol. 2025, 16, 1942–1951, doi:10.3762/bjnano.16.135

• the thickness of the precursor layer on the substrate. (2) Performing SEM imaging of the growth layers, which provide detailed information about the surface structure and composition of the precursor layer. They are essential for understanding how the precursors are decomposed under the following FIB

• island-like features become more apparent. The precursor layer underwent decomposition leading to the development of surface features enriched with metallic element of the primary film. The quantitative changes are presented in Figure 2b, which shows the mean BSE signal intensity, acquired while imaging

PEGylated lipids in lipid nanoparticle delivery dynamics and therapeutic innovation

• Peiyang Gao

Beilstein J. Nanotechnol. 2025, 16, 1914–1930, doi:10.3762/bjnano.16.133

• , respectively, 18.9-fold and 17.3-fold higher luciferase expression compared to 5 mol % PEG LNPs. Fundus imaging and immunohistochemistry confirmed robust tdTomato fluorescence in the retinal pigment epithelium (RPE), with a twofold to 3.4-fold increase in signal, showing the 0.5% PEG LNPs consistently

• . Imaging and histological analysis showed that maleimide-LNPs remained localized near the injection site, implying enhanced interaction with the tumor microenvironment. In contrast, carboxylic acid-LNPs showed a reduced cellular association, likely due to electrostatic repulsion [52]. PDP-LNPs had minimal