Electrokinetic characterization of synthetic protein nanoparticles

• Daniel F. Quevedo,
• Cody J. Lentz,
• Adriana Coll de Peña,
• Yazmin Hernandez,
• Nahal Habibi,
• Rikako Miki,
• Joerg Lahann and
• Blanca H. Lapizco-Encinas

Beilstein J. Nanotechnol. 2020, 11, 1556–1567, doi:10.3762/bjnano.11.138

• variety of different particle and fiber architectures can be made [54][55]. Bovine serum albumin (BSA) SPNPs were synthesized via the EHD co-jetting method, with BSA as the protein and an oligomer composed of poly(ethylene glycol) (PEG, 2 kDa) terminally bifunctionalized with N-hydroxysuccinimide (NHS

Transient coating of γ-Fe₂O₃ nanoparticles with glutamate for its delivery to and removal from brain nerve terminals

• Konstantin Paliienko,
• Artem Pastukhov,
• Michal Babič,
• Daniel Horák,
• Olga Vasylchenko and
• Tatiana Borisova

Beilstein J. Nanotechnol. 2020, 11, 1381–1393, doi:10.3762/bjnano.11.122

• the nanoparticles [26]. Abakumov et al. revealed that nanoparticles coated with bovine serum albumin can be used for glioma visualization and drug delivery of anticancer therapeutics [34]. Analysis of the biocoating formation at the surface of nanoparticles is crucial for understanding the mechanisms

Thermophoretic tweezers for single nanoparticle manipulation

• Jošt Stergar and
• Natan Osterman

Beilstein J. Nanotechnol. 2020, 11, 1126–1133, doi:10.3762/bjnano.11.97

• with an aqueous solution of bovine serum albumin (Sigma-Aldrich) and washed to prevent nanoparticle adhesion to the glass. A collimated IR laser beam (Compass 1064-2500MN, Coherent, λ = 1064 nm) is deflected with a pair of acousto-optic deflectors (AODs). AODs enable the simultaneous generation of

Key for crossing the BBB with nanoparticles: the rational design

• Sonia M. Lombardo,
• Marc Schneider,
• Akif E. Türeli and
• Nazende Günday Türeli

Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72

• cationic bovine serum albumin (CBSA) allows them to cross the BBB by AMT, as described above [110][111][112][114]. PEG-PLA nanoparticles were labeled with coumarin-6 and conjugated with either CBSA or BSA in a study by Lu and co-workers [110]. Transcytosis assays were performed on a co-culture BBB in vitro

Identification of physicochemical properties that modulate nanoparticle aggregation in blood

• Ludovica Soddu,
• Duong N. Trinh,
• Eimear Dunne,
• Dermot Kenny,
• Giorgia Bernardini,
• Ida Kokalari,
• Arianna Marucco,
• Marco P. Monopoli and
• Ivana Fenoglio

Beilstein J. Nanotechnol. 2020, 11, 550–567, doi:10.3762/bjnano.11.44

• , custom parallel plate perfusion chambers were coated overnight with 100 μg/mL VWF, washed with phosphate-buffered saline and blocked with 1% bovine serum albumin for 1 h prior to use. Whole blood was labelled with 1 μM DiOC6 (Sigma-Aldrich, Ireland) for 5 min at 37 °C prior to perfusion through the

Luminescent gold nanoclusters for bioimaging applications

• Nonappa

Beilstein J. Nanotechnol. 2020, 11, 533–546, doi:10.3762/bjnano.11.42

• were observed in N-acetylglutathione (NAGSH) and N-formylglutathione (NFGSH) ligands. Xie and co-workers reported the synthesis of a bovine serum albumin (BSA)-protected water-soluble Au25 nanocluster (Au-BSA) having a red emission at ca. 640 nm with a PL quantum yield of 6% (Figure 1E,F) [63]. More

• : glutathione; NAC: N-acetylcysteine; MTU: 5-methyl-2-thiouracil). D) Structures of biomolecular ligands (HSA: human serum albumin, PDB ID IE78; BSA: bovine serum albumin, PDB ID 3VO3, and Lys: lysozyme, PDB ID 3WUN). E) Schematic representation showing Au-BSA NC synthesis. F) Absorbance and fluorescence

Multilayer capsules made of weak polyelectrolytes: a review on the preparation, functionalization and applications in drug delivery

• Varsha Sharma and
• Anandhakumar Sundaramurthy

Beilstein J. Nanotechnol. 2020, 11, 508–532, doi:10.3762/bjnano.11.41

• PE capsules by changing the open and closed state of the capsules. PAH/PMA microcapsules were successfully loaded with fluorescein isothiocyanate-bovine serum albumin (FITC-BSA) at pH < 4 [70]. Confocal microscopy was used to visualize the open and closed state of the capsules at pH 3 and 7

Preparation and in vivo evaluation of glyco-gold nanoparticles carrying synthetic mycobacterial hexaarabinofuranoside

• Gennady L. Burygin,
• Polina I. Abronina,
• Nikita M. Podvalnyy,
• Sergey A. Staroverov,
• Leonid O. Kononov and
• Lev A. Dykman

Beilstein J. Nanotechnol. 2020, 11, 480–493, doi:10.3762/bjnano.11.39

• -called weak antigens (haptens), i.e., a pronounced immune response against them is not developed (in general, substances with molecular mass ca. 1 kDa or less are not immunogenic). As a rule, haptens are conjugated with carrier proteins, such as bovine serum albumin, hemocyanin, ovalbumin, or

Rational design of block copolymer self-assemblies in photodynamic therapy

• Maxime Demazeau,
• Laure Gibot,
• Anne-Françoise Mingotaud,
• Patricia Vicendo,
• Clément Roux and
• Barbara Lonetti

Beilstein J. Nanotechnol. 2020, 11, 180–212, doi:10.3762/bjnano.11.15

• negatively charged catalase or bovine serum albumin and positively charged chitosan or poly(allylamine)-coated MnO2 have been exploited to obtain pH-sensitive nanovectors [68][69]. The low concentration of endogenous H2O2 together with the instability of catalase in physiological environments containing

Use of data processing for rapid detection of the prostate-specific antigen biomarker using immunomagnetic sandwich-type sensors

• Camila A. Proença,
• Tayane A. Freitas,
• Thaísa A. Baldo,
• Elsa M. Materón,
• Flávio M. Shimizu,
• Gabriella R. Ferreira,
• Frederico L. F. Soares,
• Ronaldo C. Faria and
• Osvaldo N. Oliveira Jr.

Beilstein J. Nanotechnol. 2019, 10, 2171–2181, doi:10.3762/bjnano.10.210

• . Reduced ʟ-glutathione (GSH, 99%), bovine serum albumin (BSA), HAuCl4·3H2O (99.9%), sodium borohydride (99%), horseradish peroxidase (HRP, MW = 44000; 250–330 u·mg−1), poly(diallydimethylammonium chloride) (PDDA, 20 wt % in H20), 1-(3-(dimethylamino)propyl)-3-ethylcarbodiimide hydrochloride (EDC), N

• PBS and incubated with 5 μL of bovine serum albumin (BSA) (2% w/w) diluted in PBS to avoid non-specific binding (NBS). Each step of the modification was monitored with polarization-modulated infrared reflection absorption spectroscopy (PM-IRRAS, see Figure S1F in Supporting Information File 1). The

• incubated with 5 μL of bovine serum albumin (BSA) (2% w/w) diluted in phosphate-buffered saline (PBS), to avoid non-specific binding. Preparation of the bioconjugate complex of Ab2 and HRP (Ab2-MNP-HRP). Illustration of the PSA capturing step. Comparison of various sandwich-type immunosensors and

Incorporation of doxorubicin in different polymer nanoparticles and their anticancer activity

• Sebastian Pieper,
• Hannah Onafuye,
• Dennis Mulac,
• Jindrich Cinatl Jr.,
• Mark N. Wass,
• Martin Michaelis and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 2062–2072, doi:10.3762/bjnano.10.201

• (vinyl alcohol) (PVA, 30,000–70,000 Da), bovine serum albumin (BSA), HSA, and glutaraldehyde were obtained from Sigma-Aldrich Chemie GmbH (Karlsruhe, Germany). Dulbecco's Phosphate buffered saline (PBS) was purchased from Biochrom GmbH (Berlin, Germany). Doxorubicin was obtained from LGC Standards GmbH

• % (m/v) bovine serum albumin (BSA) was shaken at 37 °C with 500 rpm. Nanoparticle suspensions were centrifuged (30,000g, 15 min) after 0, 0.5, 1, 2, 4, 6, 8, and 24 h, and an aliquot (250 µL) of the supernatant was diluted with 750 µL ethanol (96%, v/v) in order to precipitate BSA. After a second

Gold-coated plant virus as computed tomography imaging contrast agent

• Alaa A. A. Aljabali,
• Mazhar S. Al Zoubi,
• Khalid M. Al-Batanyeh,
• Ali Al-Radaideh,
• Mohammad A. Obeid,
• Abeer Al Sharabi,
• Walhan Alshaer,
• Bayan AbuFares,
• Tasnim Al-Zanati,
• Murtaza M. Tambuwala,
• Naveed Akbar and
• David J. Evans

Beilstein J. Nanotechnol. 2019, 10, 1983–1993, doi:10.3762/bjnano.10.195

• carbonate, sucrose, dimethyl sulfoxide (DMSO; 25%), 2-(N-morpholino)ethanesulfonic acid (MES) buffer, isopropanol, tetrachloroauric acid, trypsin, ethylenediaminetetraacetic acid (EDTA), HEPES sodium salt, Triton™ X-100, phosphate-buffered saline (PBS) tablets, and bovine serum albumin (BSA) were purchased

Magnetic properties of biofunctionalized iron oxide nanoparticles as magnetic resonance imaging contrast agents

• Natalia E. Gervits,
• Andrey A. Gippius,
• Alexey V. Tkachev,
• Evgeniy I. Demikhov,
• Sergey S. Starchikov,
• Igor S. Lyubutin,
• Alexander L. Vasiliev,
• Vladimir P. Chekhonin,
• Maxim A. Abakumov,
• Alevtina S. Semkina and
• Alexander G. Mazhuga

Beilstein J. Nanotechnol. 2019, 10, 1964–1972, doi:10.3762/bjnano.10.193

• functionalization of nanoparticles with bovine serum albumin (BSA) and human serum albumin (HSA) is one potential method to enhance their biocompatibility. There are several directions in the development of coating types: coating of the nanoparticles themselves [10], design of BSA microcapsules with iron oxide

Toxicity and safety study of silver and gold nanoparticles functionalized with cysteine and glutathione

• Barbara Pem,
• Igor M. Pongrac,
• Lea Ulm,
• Ivan Pavičić,
• Valerije Vrček,
• Darija Domazet Jurašin,
• Marija Ljubojević,
• Adela Krivohlavek and
• Ivana Vinković Vrček

Beilstein J. Nanotechnol. 2019, 10, 1802–1817, doi:10.3762/bjnano.10.175

• joined, centrifuged at 800g for 5 min and washed with PBS containing 2% bovine serum albumin (1 mL per sample). The cells were then stained using Muse® Annexin V and a dead cell assay kit (Merck KGaA, Darmstadt, Germany) according to the manufacturer’s instructions. After staining, the cells were washed

Enhanced inhibition of influenza virus infection by peptide–noble-metal nanoparticle conjugates

• Zaid K. Alghrair,
• David G. Fernig and
• Bahram Ebrahimi

Beilstein J. Nanotechnol. 2019, 10, 1038–1047, doi:10.3762/bjnano.10.104

• agarose overlay was prepared by mixing equal volumes of 2% (w/v) of pre-warmed (55 °C) low-melting agarose (Melford Laboratories Ltd, Blideston Road, Ipswich, UK) and the overlay solution (14 mL 10× MEM, 3.7 mL 7.5% (w/v) bovine serum albumin (fraction V, Sigma-Aldrich, UK), 1.4 mL L-glutamine, 2.6 mL 7.5

Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• (Karlsruhe, Germany). The dye Coomassie Brilliant Blue G-250 was purchased from VWR Life science AMRESCO (Solon, Ohio). Bovine serum albumin (BSA), DL-dithiothreitol (DTT), and iodoacetamide (IAA) were obtained from Sigma-Aldrich (Steinheim, Germany). Trypsin (sequencing grade) for protein digestion was

Experimental study of an evanescent-field biosensor based on 1D photonic bandgap structures

• Jad Sabek,
• Francisco Javier Díaz-Fernández,
• Luis Torrijos-Morán,
• Zeneida Díaz-Betancor,
• Ángel Maquieira,
• María-José Bañuls,
• Elena Pinilla-Cienfuegos and
• Jaime García-Rupérez

Beilstein J. Nanotechnol. 2019, 10, 967–974, doi:10.3762/bjnano.10.97

• to bovine serum albumin (BSA) using a UV-induced immobilization procedure. The use of half-antibodies allows one to reduce the thickness of the biorecognition volume down to ca. 2.5 nm, thus leading to a higher interaction with the evanescent field, as well as a proper orientation of their binding

• work we have used an off-flow spotting process for the immobilization of the half-antibodies, which yields a higher surface coverage and thus significantly higher sensing responses, as demonstrated in the reported bovine serum albumin (BSA) detection experiments. Results and Discussion PBG sensing

Ceria/polymer nanocontainers for high-performance encapsulation of fluorophores

• Kartheek Katta,
• Dmitry Busko,
• Yuri Avlasevich,
• Katharina Landfester,
• Stanislav Baluschev and
• Rafael Muñoz-Espí

Beilstein J. Nanotechnol. 2019, 10, 522–530, doi:10.3762/bjnano.10.53

• materials. The core–shell structure of the hybrid organic–inorganic nanoparticles allows for the independent molecular design of each part. For instance, the oxygen permeability of the shell material can be lowered drastically by using semicrystalline nanocellulose [31]. Furthermore, a bovine serum albumin

The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa

• Birgit Lengerer,
• Marie Bonneel,
• Mathilde Lefevre,
• Elise Hennebert,
• Philippe Leclère,
• Emmanuel Gosselin,
• Peter Ladurner and
• Patrick Flammang

Beilstein J. Nanotechnol. 2018, 9, 2071–2086, doi:10.3762/bjnano.9.196

• and rehydration, antigen retrieval with a solution of 0.05% trypsin and 0.1% CaCl2 was performed for 15 min on 37 °C. Footprints were collected on microscope glass slides and fixed in 4% PFA in PBS overnight at room temperature. All samples were blocked in PBS containing 3% (w/v) bovine serum albumin

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

Mechanistic insights into plasmonic photocatalysts in utilizing visible light

• Kah Hon Leong,
• Azrina Abd Aziz,
• Lan Ching Sim,
• Pichiah Saravanan,
• Min Jang and
• Detlef Bahnemann

Beilstein J. Nanotechnol. 2018, 9, 628–648, doi:10.3762/bjnano.9.59

• occurs in microorganisms because it is fast and cost effective and can be readily scaled for realistic applications [163]. A very recent study demonstrated that Ag/AgCl can be synthesized using ash gourd peel extract without using organic toxic solvents [157]. Bovine serum albumin (BSA) and Neem extract

Wafer-scale bioactive substrate patterning by chemical lift-off lithography

• Chong-You Chen,
• Chang-Ming Wang,
• Hsiang-Hua Li,
• Hong-Hseng Chan and
• Wei-Ssu Liao

Beilstein J. Nanotechnol. 2018, 9, 311–320, doi:10.3762/bjnano.9.31

• (TRIS) and tris(2-carboxyethyl)phosphine (TCEP) were obtained from Acros Organics (Geel, Belgium). Bovine serum albumin (BSA), and 10× phosphate buffered saline (PBS) containing 1.37 M NaCl, 0.027 M KCl, 0.10 M Na2HPO4, and 0.018 M KH2PO4 were purchased from Bioman Scientific Co., Ltd (Taipei, Taiwan

Strategy to discover full-length amyloid-beta peptide ligands using high-efficiency microarray technology

• Clelia Galati,
• Natalia Spinella,
• Lucio Renna,
• Danilo Milardi,
• Francesco Attanasio,
• Michele Francesco Maria Sciacca and
• Corrado Bongiorno

Beilstein J. Nanotechnol. 2017, 8, 2446–2453, doi:10.3762/bjnano.8.243

• our laboratory. Experimental Chemicals Phosphate-buffered saline, hydrogen peroxide (29%), ammonium hydroxide (25%), hydrochloric acid (37%), methanol, dimethyl sulfoxide (DMSO), anhydrous toluene and 3-glycidyloxypropyltrimethoxysilane (GOPs) were acquired from Sigma-Aldrich. Bovine serum albumin

Low uptake of silica nanoparticles in Caco-2 intestinal epithelial barriers

• Dong Ye,
• Mattia Bramini,
• Delyan R. Hristov,
• Sha Wan,
• Anna Salvati,
• Christoffer Åberg and
• Kenneth A. Dawson

Beilstein J. Nanotechnol. 2017, 8, 1396–1406, doi:10.3762/bjnano.8.141

• . Caco-2 cells were fixed and permeabilised with methanol for 4 min at −20 °C and then carefully washed with PBS three times. Antibody unspecific binding was prevented by treating the cells with a blocking buffer of 1% bovine serum albumin (BSA)-Tween PBS for 30 min (Tween 0.05%, v/v). To stain the

Evaluation of quantum dot conjugated antibodies for immunofluorescent labelling of cellular targets

• Jennifer E. Francis,
• David Mason and
• Raphaël Lévy

Beilstein J. Nanotechnol. 2017, 8, 1238–1249, doi:10.3762/bjnano.8.125

• × in PBS (5 min), permeabilised with 0.25% Triton X-100 in PBS for 60 min (except methanol fixation), washed again 3× in PBS (5 min), and incubated with 6% bovine serum albumin (BSA, Sigma-Aldrich, UK) in PBS (60 min). Primary antibodies produced in mouse (anti-β-tubulin TUB 2.1, Sigma-Aldrich, UK