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Search for "fluorescence" in Full Text gives 422 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.
Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent
Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46
- nanovesicles, RhPE at 0.4 μg per mg of lipids was added to the mixture of lipids, and lipid films were hydrated with Tris buffer as stated above. RhPE was quantified by spectrofluorometry (λex = 561 nm and λem = 580 nm) with an LS55 fluorescence spectrometer (PerkinElmer Inc. MA, USA) upon complete disruption
- of 1 volume of nanovesicles in 10 volumes of methanol. The fluorescence intensity of the sample was compared with a standard curve prepared with RhPE in methanol. The standard curve was linear in the range of 0.075–0.5 μg/mL RhPE. Cells and culture conditions Human umbilical vein endothelial cells
- monocytes and trypsinized macrophages were washed once with 300 µL PBS, and a total of 1 × 104 cells were analyzed by flow cytometry (BD FACSCalibur™; BD Biosciences, San Jose, CA, USA). The fluorescence was normalized to the RhPE/TL ratio of each formulation. Data were analyzed using Flowing Software 2.5.1
Photocatalytic degradation of methylene blue under visible light by cobalt ferrite nanoparticles/graphene quantum dots
Beilstein J. Nanotechnol. 2024, 15, 475–489, doi:10.3762/bjnano.15.43
- /GQDs nanoparticles. Magnetic properties, morphology, structure, and fluorescence of the nanocomposites were studied, and the photocatalytic degradation of methylene blue as a dye model and the mechanism of methylene degradation were also addressed. Experimental Materials Cobalt(II) nitrate hexahydrate
Potential of a deep eutectic solvent in silver nanoparticle fabrication for antibiotic residue detection
Beilstein J. Nanotechnol. 2024, 15, 426–434, doi:10.3762/bjnano.15.38
- scholars, the rod-like morphology is better than a spherical one at increasing the extinction coefficient, about 109 to 1011 M−1·cm−1 higher [43][44], which proves the applicability of Ag NPs-DES in SERS biosensors. Furthermore, X-ray fluorescence mapping was used to evaluate the presence of silver in the
- the nanostructure and surface morphology of the nanoparticles, as well as the elemental distribution of silver on the substrate, a S4800 field-emission scanning electron microscope purchased from Hitachi, Japan, and an M4 TORNADOPlus Micro X-ray fluorescence spectrometer with a Rh tube at 30 W micro
Classification and application of metal-based nanoantioxidants in medicine and healthcare
Beilstein J. Nanotechnol. 2024, 15, 396–415, doi:10.3762/bjnano.15.36
- radiotherapy efficacy. Also, using Au/AgNDs for fluorescence images could distinguish tumors from normal tissue, thus, assisting surgeons during tumor resection, which is a novel strategy and vision for the clinical diagnosis and treatment of cancer. Cardiovascular diseases Cardiovascular diseases (CVDs
Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics
Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17
- formulations of the three nanoparticles were F127-folate@PLGA/IO/CHL/IR780 (F127-folate@NP), F127@PLGA/IO/CHL/IR780 (F127@NP), and PVA@PLGA/IO/CHL/IR780 (PVA@NP). Coumarin-6 (0.2 mg) was added to the organic phase to create F127-folate@NP/Cou-6, F127@NP/Cou-6, and PVA@NP/Cou-6 for the fluorescence assay in
- . Then, the signals were normalized to the signal of PVA@NP/Cou-6 for comparison. The fluorescence image of the NPs in the cells were displayed in Supporting Information File 1, Supplementary data 2 and Figure S2. Cytotoxicity effects of nanoparticles The cells were seeded onto 96-well plates at 5,000
- stronger fluorescence signals than cells treated with PVA@NP/Cou-6 (Figure 3). This was true for all four types of cells. In 3T3, HEK, and MCF-7 cells, the signal of F127-folate@NP/Cou-6 and F127@NP/Cou-6 was 1.4 times stronger than that of PVA@NP/Cou-6. However, in HepG2 cells, it was only 1.2 times
CdSe/ZnS quantum dots as a booster in the active layer of distributed ternary organic photovoltaics
Beilstein J. Nanotechnol. 2024, 15, 144–156, doi:10.3762/bjnano.15.14
- nm for QD580, 596 nm for QD600, and 636 nm for QD640. The full width at half maximum values of the fluorescence peaks increase, and they are about 30–40 nm. The luminescence peak for QD640 is significantly wider at about 50 nm. Figure 4 shows the absorption and luminescence spectra of mixtures of
- refractive index, which decreases with the increasing fluorescence wavelength of the quantum dots studied. The paper by Dement et al. [45] also presents elipsometric studies, however in a narrower scope. Obtaining quantum dots was done by a different method. Nonetheless, they obtained absorption features not
Assessing phytotoxicity and tolerance levels of ZnO nanoparticles on Raphanus sativus: implications for widespread adoptions
Beilstein J. Nanotechnol. 2024, 15, 115–125, doi:10.3762/bjnano.15.11
- Fusarium wilt in Solanum melongena L. while enhancing its carbohydrates and chlorophyll contents [13]. In contrast, a significant reduction of chlorophyll fluorescence of Hordeum sativum (barley) treated with 2000 mg/L of ZnO NPs compared to 300 mg/L of ZnO NPs has also been reported [41]. These pieces of
Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface
Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8
- antibody fraction after stripping treatment (group 2) was only 11.4% compared to the reference group 1, which is 100% (Figure 1). The amount of bound antibody is indicated by the amount of fluorescein (FITC) fluorescence detected as this fluorochrome is associated with the antibody in question. It is
- measured as relative fluorescence unit (RFU) (index explained in the Experimental section). Results are expressed as the mean RFU of the replicates, and the variation is expressed as the standard error of the mean (SEM). When the immunocapture system had been reconstituted after the stripping procedure
- affected, we also wanted to determine how the immunocapture capacity of these immobilised antibodies was affected. This was determined by assessing the fluorescence associated with the immunocaptured antigen, which, in this study, was the protein toxin simulant BSA. The BSA-associated fluorochrome was
Curcumin-loaded nanostructured systems for treatment of leishmaniasis: a review
Beilstein J. Nanotechnol. 2024, 15, 37–50, doi:10.3762/bjnano.15.4
- proven by the strong intracellular fluorescence levels. Furthermore, NLCs increased the in vitro leishmanicidal activity of curc (IC50: 105.49 ± 3.71 μg/mL) when compared to curc-free NLCs (IC50: 165.06 ± 4.64 μg/mL). Similar performance occurred in vivo in axenic amastigotes, (IC50 of the curc-NLC
Fluorescent bioinspired albumin/polydopamine nanoparticles and their interactions with Escherichia coli cells
Beilstein J. Nanotechnol. 2023, 14, 1208–1224, doi:10.3762/bjnano.14.100
- and RhBITC-BSA/PDA NPs penetrated and accumulated in both cell wall and inner compartments of Escherichia coli (E. coli) cells. The fluorescence signals were diffuse or displayed aggregate-like patterns with both labelled NPs and free dyes. RhBITC-BSA/PDA NPs led to the most intense fluorescence in
- labelled BSA/PDA NPs to track bacteria and carry drugs in the core of bacterial cells. Keywords: accumulation; albumin; antibacterial; Escherichia coli; fluorescence; nanoparticles; penetration; polydopamine; Introduction Organic nanoparticles (ONPs) are used to target and deliver drugs to tissue and
- ) of poly(lactic-co-glycolic acid) (PLGA) [7], polycaprolactone [8], and chitosan [9]. Furthermore, fluorescent ONPs are a promising way to facilitate the localization of NPs in cells through fluorescence imaging. They can also be used for fluorescent labelling of cells, especially for live cell
Hierarchically patterned polyurethane microgrooves featuring nanopillars or nanoholes for neurite elongation and alignment
Beilstein J. Nanotechnol. 2023, 14, 1157–1168, doi:10.3762/bjnano.14.96
- nanopillar substrate having the smallest CAs (CA ≈ 30°). Based on confocal fluorescence microscopy of immunostained samples (Figure 1J–L), laminin successfully adsorbed onto the O2 plasma-treated PU samples. There was good laminin coverage on all of the samples, even on the nanostructures, as indicated by
- the fluorescence patterns conforming to the structure shapes. Laminin was also successfully coated on the flat areas surrounding the pillars and holes, as shown in the corresponding confocal slices in Supporting Information File 1, Figure S5. O2 plasma treatment of PU enables strong laminin adsorption
- values after plasma treatment, the lower CA [∥] values (<60°) indicate that the surface was hydrophilic enough along the groove direction, and good solution coverage could be achieved during laminin coating. We also confirmed laminin adsorption on the grooved PU samples using confocal fluorescence
Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study
Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93
- upon excitation at 488 nm (Figure 2E). This blue shift in emission maximum could be due to the binding of CUR to the hydrophobic core of HSA-MPs, confirming successful entrapment of CUR within the particles. Moreover, HSA-MPs showed no obvious emission maximum, indicating that the fluorescence came
- cellular uptake of HSA-MPs and CUR-HSA-MPs. The intrinsic fluorescence of both particle types excited at 488 nm allowed for their visualization, as the fluorescence properties were preserved upon encapsulation (Figure 2E). Quantitative analysis of cellular uptake, performed through flow cytometry, revealed
- a 1.6-fold increase in the uptake of CUR-HSA-MPs compared to HSA-MPs (Figure 8B,C). However, the mean intrinsic fluorescence intensity of HSA-MPs is significantly lower than that of CUR-HSA-MPs when excited at 488 nm, as shown in Figure 2E. Therefore, confirming the preferential uptake of CUR-HSA
Properties of tin oxide films grown by atomic layer deposition from tin tetraiodide and ozone
Beilstein J. Nanotechnol. 2023, 14, 1085–1092, doi:10.3762/bjnano.14.89
- , the cycle times for SnO2 were kept at 5-2-5-5 s, respectively, for the following sequence: metal precursor pulse, N2 purge pulse, O3 pulse, and N2 purge pulse. The films were grown on Si(100) cleansed and etched prior to the growth. An X-ray fluorescence spectrometer Rigaku ZSX 400 with the program
Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one
Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85
- (DAPI). The closely spaced emission maxima of DAPI and FITC, or PI and APC, necessitated the use of different dyes for DNA staining depending on which signal, FITC or APC, was of interest in each sample. In each run, 2000 monocytes were analyzed. The mean fluorescence intensity (MFI) of the reference
- within one run (expressed as percentages). Then the arithmetic means over three experimental runs were calculated from these normalized MFI values. Furthermore, to identify the monocyte population in fluorescence-activated cell sorting (FACS) analysis, whole blood samples were each incubated with FITC
- showed no aggregation within the particle suspension. Exclusion of confounders, indirect phagocytosis test HbMPs showed a low autofluorescence in the FITC channel in FACS analysis, which did not influence the fluorescence of DAPI in the PI channel. Whole blood (wb) generated a very low FITC signal as
Nanoarchitectonics of photothermal materials to enhance the sensitivity of lateral flow assays
Beilstein J. Nanotechnol. 2023, 14, 988–1003, doi:10.3762/bjnano.14.82
- spectroscopy (SERS), fluorescence, ultrasound, and photothermal methods, have been tried in LFAs [4][13][14]. For example, fluorescence-based LFAs exhibited a 100- to 1000-fold higher sensitivity than conventional LFAs [15]. However, working with fluorescence-based LFAs is complex because of photoinstability
- , SERS, fluorescence, and photothermal applications [45]. For instance, compared to spherical architectures, anisotropic nanoscale architectures, such as nanostars, nanoflowers, nanopopcorns, nanorods, and nanocages, exhibit superior SERS and photothermal conversion activities [46][47]. Therefore, it is
- reduced graphene oxide (rGO), SERS imaging can be done along with photothermal therapy [84]. Recently, our group developed a multifunctional rGO–Au nanoscale architecture loaded with Raman dye and anticancer drugs for fluorescence/SERS imaging-guided breast cancer therapy. Under activation of a laser at
Isolation of cubic Si3P4 in the form of nanocrystals
Beilstein J. Nanotechnol. 2023, 14, 971–979, doi:10.3762/bjnano.14.80
- nanoparticles with a defective zinc blende structure under mild conditions through thermal annealing of hydrogenated silicon nanoparticles with red phosphorus. The synthesized Si3P4 nanoparticles were analyzed using FTIR, XRD, electron diffraction, EDX, TEM, Raman spectroscopy, X-ray fluorescence spectrometry
- “WinXPow”. Elemental analysis was carried out on an X-ray fluorescence spectrometer S2 Picofox (Bruker) with total external reflection. Transmission electron microscopy (TEM) samples were dispersed in an ultrasonic bath with hexane and then deposited on copper grids with lacey carbon film (SPI Supplies
- -ray fluorescence analyses and XRD analyses of samples annealed with phosphorus at 670, 400, and 900 °C. Calculated frequencies of optical phonons (cm−1) and preliminary designation of the experimentally observed vibrational frequencies of Si3P4 NPs. Supporting Information Supporting Information File
Silver-based SERS substrates fabricated using a 3D printed microfluidic device
Beilstein J. Nanotechnol. 2023, 14, 793–803, doi:10.3762/bjnano.14.65
- [40][41][42]. Silicon nanostructures with high specific areas are especially popular because they have no fluorescence properties. In this work, we report on the synthesis of a highly sensitive SERS substrate for detecting rhodamine B (RhB) and melamine (MLM) and on the analytical properties of the
A graphene quantum dots–glassy carbon electrode-based electrochemical sensor for monitoring malathion
Beilstein J. Nanotechnol. 2023, 14, 701–710, doi:10.3762/bjnano.14.56
- obtained, which will be used as an electrochemical nanosensor in further studies for malathion detection (Figure 1). Characterization FTIR, Raman, UV–vis, and fluorescence spectroscopy measurements were carried out to determine the optical properties of GQDs. A Perkin Elmer LAMBDA 750 spectrophotometer was
- used to record the UV–visible absorbance spectrum. The luminescence characteristics of the GQDs were investigated using a Perkin Elmer LS 55 fluorescence spectrometer. On a Perkin Elmer FT-IR Spectrum 2, FTIR spectra were measured in the range of 500–4000 cm−1 by making KBr pellets of the sample. At
Metal-organic framework-based nanomaterials as opto-electrochemical sensors for the detection of antibiotics and hormones: A review
Beilstein J. Nanotechnol. 2023, 14, 631–673, doi:10.3762/bjnano.14.52
- that are time-consuming and require experienced professionals. Metal-organic frameworks (MOFs) with variable porosity, active functional sites, and fluorescence capacity are attractive materials for developing opto-electrochemical sensors. Herein, the insights into the capabilities of electrochemical
- , multistep separation procedure. A fluorescence polarisation immunoassay, which does not require a separation step, is one development that has been made to get around this restriction [22]. Furthermore, chromatographic techniques such as thin-layer chromatography (TLC), liquid chromatography-coupled mass
- as the necessity for technical experts with the necessary training, the limited selectivity and reusability of substrates, and the degradation of substrates with time, which reduces the signal. Several portable electrochemical and optical (chemiluminescence, fluorescence, and electrochemiluminescence
Titania nanoparticles for photocatalytic degradation of ethanol under simulated solar light
Beilstein J. Nanotechnol. 2023, 14, 616–630, doi:10.3762/bjnano.14.51
- fluorescent compound, namely umbelliferone, was monitored with a Carry Eclipse fluorescence spectrometer, slits set to 10 nm in excitation and emission, λexc = 330 nm. The experimental procedure for photocatalytic tests started with dispersing a uniform layer of 0.01 g of titania photocatalyst nanopowder on
Carbon nanotube-cellulose ink for rapid solvent identification
Beilstein J. Nanotechnol. 2023, 14, 535–543, doi:10.3762/bjnano.14.44
- fluorescence spectroscopy, and inductively coupled plasma mass spectrometry (ICP-MS). These techniques are complex, expensive, and require experts to carry them out. Also, they often require several pretreatment steps with high-cost materials [10][11]. Electronic tongues are a category of liquid sensors that
The origin of black and white coloration of the Asian tiger mosquito Aedes albopictus (Diptera: Culicidae)
Beilstein J. Nanotechnol. 2023, 14, 496–508, doi:10.3762/bjnano.14.41
- analysed using scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. Reflectance spectra of the white areas are measured. No clear difference is present in the morphology of micro- and nanostructures of black and white scales in SEM and TEM, but black scales contain a
- . The ultrastructure of the white and black scales on the hindlegs of Ae. albopictus is analysed using scanning electron microscopy, transmission electron microscopy, and fluorescence microscopy. Moreover, reflectance spectra of the white areas are measured. The scales are present also on other body
- dry adults using a fluorescence microscope revealed that the white areas on the tarsi show UV-induced fluorescence (Figure 8). The excitation occurs at 365 nm (UV light) and the emission from 397 nm (blue light). Discussion Multifunctional role of the scales The present investigation reveals that the
A mid-infrared focusing grating coupler with a single circular arc element based on germanium on silicon
Beilstein J. Nanotechnol. 2023, 14, 478–484, doi:10.3762/bjnano.14.38
- , transparent, and portable wrist strap sensor and a mechano-based transductive sensor in 2017 and 2018, respectively [16][17]. They have good application prospects in healthcare. In 2020, Xue et al. reported a bismuthene-enabled fluorescence quenching biosensor to detect microRNA, which is relevant to the
The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy
Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30
- ) [16]. Unfortunately, confocal imaging is limited by relatively low throughput (even with automation) and can be ambiguous when determining internalization within 500 nm of the cell membrane [17]. However, widefield fluorescence microscopy is still widely used when it comes to observing the expression
- after image capture (26%, Figure 1a2). Flow cytometry, also a fluorescence-based method, is an alternate assessment of uptake and transfection. It is commonly used for high-throughput cell studies and is known for both rapid data acquisition and large data sets [17]. Flow cytometry can generate large
- surface-bound and internalized NPs. Commonly used techniques are far from optimal, but include non-specific methods for removing surface-bound NPs or quenching surface-bound fluorescence [17]. In addition to the distinction between membrane binding and internalization, there are other concerns associated
Polymer nanoparticles from low-energy nanoemulsions for biomedical applications
Beilstein J. Nanotechnol. 2023, 14, 339–350, doi:10.3762/bjnano.14.29
- evaporation protocols allowed Generalova et al. [39] to prepare ethyl cellulose nanoparticles containing both hydrophobic and hydrophilic fluorescent nanocrystals. Size measurements showed that nanoparticles were in the range of 128–132 nm. Additionally, the corresponding fluorescence-labeled nanoparticles
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