Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease

• Niédja Fittipaldi Vasconcelos,
• Almerinda Agrelli,
• Rayane Cristine Santos da Silva,
• Carina Lucena Mendes-Marques,
• Isabel Renata de Souza Arruda,
• Priscilla Stela Santana de Oliveira,
• Mércia Liane de Oliveira and
• Giovanna Machado

Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120

• , thus destabilizing bilayer formation. Conversely, lecithins with a higher proportion of saturated phospholipids are more likely to organize into stable bilayer vesicles like liposomes. These structural features of lecithin play a crucial role in determining the physicochemical properties of the

• using R v.4.5.1 [60], specifically the packages “tidyverse” v.2.0.0 [61] and “rstatix v.0.7.2 [62]. Supplementary statistical data are provided in spreadsheets and can be accessed in Supporting Information File 2. Results and Discussion Physicochemical properties of linseed oil The identification and

• optimal surfactant utilization. Finally, the third phase (illustrated by pre-formulations O/W(L-4%) and O/W(L-5%)) manifests an overabundance of lecithin, resulting in the formation of micellar structures within the pre-formulations. As electrical conductivity is a physicochemical parameter modulated by

Prospects of nanotechnology and natural products for cancer and immunotherapy

• Jan Filipe Andrade Santos,
• Marcela Bernardes Brasileiro,
• Pamela Danielle Cavalcante Barreto,
• Ligiane Aranha Rocha and
• José Adão Carvalho Nascimento Júnior

Beilstein J. Nanotechnol. 2025, 16, 1644–1667, doi:10.3762/bjnano.16.116

• immunotherapy is nanotechnology, as it presents physicochemical benefits for drugs. Among the advantages, nanotechnology can eliminate the limitations of using bioactive compounds in cancer treatment formulations, leading to increased bioavailability and pharmacokinetics [55]. The mechanisms behind the

• containing immunomodulators and checkpoint inhibitors to achieve a synergistic effect and consequently expand the clinical potential of nanotechnology containing natural products. Patent KR20220169108 was discussed in the article published by Dhandapani et al., which addresses the synthesis, physicochemical

• compounds must have properties that contribute to a stable formulation, difficulty in controlling physicochemical characteristics, such as particle size, which can affect reproducibility and scalability, and problems related to rapid clearance and interaction with non-target tissues, mainly due to the

Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion

• Philippe de Castro Mesquita,
• Karla Samara Rocha Soares,
• Manoela Torres-Rêgo,
• Emanuell dos Santos-Silva,
• Mariana Farias Alves-Silva,
• Alianda Maira Cornélio,
• Matheus de Freitas Fernandes-Pedrosa and
• Arnóbio Antônio da Silva-Júnior

Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115

• physicochemical characterization of the nanoparticles revealed mean diameters below 230 nm, positive zeta potentials, and PDI below 0.3, corroborating other reports of PLA–PEI systems [14][38]. In addition, the high encapsulation efficiency can be attributed to the functionalizing agent (PEI), which demonstrates

• routes are very common for the administration of proteins with pharmacological activity and with particle sizes of around 200 nm [40]. Regarding stability, only minimal changes in physicochemical parameters were observed, indicating colloidal stability of the nanoparticle formulation. This is

• probably explain the immune response improvement since different nanoparticle types generated stronger Th1 and Th2 immune responses compared to other antigen types [45]. Although the present study provides physicochemical and immunological data supporting the potential of PLA–PEI nanoparticles as antigen

Nanotechnology-based approaches for the removal of microplastics from wastewater: a comprehensive review

• Nayanathara O Sanjeev,
• Manjunath Singanodi Vallabha and
• Rebekah Rubidha Lisha Rabi

Beilstein J. Nanotechnol. 2025, 16, 1607–1632, doi:10.3762/bjnano.16.114

• high water flux of 196.7 L·h−1·m−2·kPa−1 was recorded, surpassing or matching the performance of most membranes fabricated from unmodified two-dimensional nanomaterials. The material exhibits physicochemical stability, excellent permeability, and superior MP removal capabilities; hence, h-Ti3C2Tx

Laser processing in liquids: insights into nanocolloid generation and thin film integration for energy, photonic, and sensing applications

• Akshana Parameswaran Sreekala,
• Pooja Raveendran Nair,
• Jithin Kundalam Kadavath,
• Bindu Krishnan,
• David Avellaneda Avellaneda,
• M. R. Anantharaman and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 1428–1498, doi:10.3762/bjnano.16.104

• al. [2] in one of the first reviews on laser ablation published in 2004. Their discussion addresses the physicochemical insights into key stages of these processes, including the behavior of the laser target, the formation of the plume, and the initial laser–target interactions. It offers a

• obtained. These mechanisms play a crucial role in determining the characteristics of the final products, whether they be spherical NPs, QDs, or other nanostructures, depending on the specific goals of the synthesis. NPs exhibit distinct mechanical and physicochemical properties that are influenced by

Photochemical synthesis of silver nanoprisms via green LED irradiation and evaluation of SERS activity

• Tuan Anh Mai-Ngoc,
• Nhi Kieu Vo,
• Cong Danh Nguyen,
• Thi Kim Xuan Nguyen and
• Thanh Sinh Do

Beilstein J. Nanotechnol. 2025, 16, 1417–1427, doi:10.3762/bjnano.16.103

• -MBA) as a probe molecule. In this study, AgNPrs were synthesized photochemically using green LED 10 W irradiation. Seeds were prepared using 1 mM AgNO3 and stabilized with a trisodium citrate (TSC)/AgNO3 molar ratio of 5. Physicochemical analyses confirmed the successful formation of AgNPrs with high

The role of biochar in combating microplastic pollution: a bibliometric analysis in environmental contexts

• Tuan Minh Truong Dang,
• Thao Thu Thi Huynh,
• Guo-Ping Chang-Chien and
• Ha Manh Bui

Beilstein J. Nanotechnol. 2025, 16, 1401–1416, doi:10.3762/bjnano.16.102

• wastewater [18][19][20][21]. Recent studies exploring the use of BC for MP remediation have yielded promising results, further emphasizing its potential for addressing environmental contamination. Recent evaluations of MP removal methods have classified biological, physicochemical, and biochar-based

• 2022, reflecting efforts to mitigate MP pollution through biochar application in terrestrial and aquatic environments. From 2022 to 2024, terms like “community structure”, “agriculture”, and “impact” suggest a shift toward investigating biochar's influence on the physicochemical properties of soil and

• physicochemical properties, remediation efficiency, and environmental stability of MPs. Evaluating biochar’s potential for MP remediation in agricultural soils, focusing on improvements in soil properties, crop yield, gene expression, and microbial communities. Examining biochar’s adsorption mechanisms and MP

Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems

• Alejandro Llamedo,
• Marina Cano,
• Raquel G. Soengas and
• Francisco J. García-Alonso

Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98

• delivery mechanism are broadly categorized into passive and active targeting [38]. Passive targeting is controlled by size, charge, and composition of the nanoparticle, which influences the localization, cell penetration, and release of the drug as physicochemical features of pathogenic tissues facilitate

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• hydrophobic phospholipid bilayer and a hydrophilic core. Depending on the physicochemical properties of the drug, this type of structure allows the entrapment of both hydrophilic and hydrophobic drugs [1]. Liposomes, like other nanosystems, have many benefits, such as prolonged systemic blood circulation

• physicochemical properties of liposomes, including size, surface charge, EE, and stability, directly influence their biological interactions and therapeutic potential. These characteristics are particularly crucial in designing liposomes for ferroptosis induction, as they determine the efficiency of iron delivery

• delivery. Effective lipid binding of medications and genes is necessary to overcome this obstacle [126]. Moreover, the increasing number of physicochemical variables in liposomal formulations makes it difficult to evaluate their pharmacokinetics, pharmacodynamics, and toxicology after administration, which

Acrocomia aculeata oil-loaded nanoemulsion: development, anti-inflammatory properties, and cytotoxicity evaluation

• Verónica Bautista-Robles,
• Hady Keita,
• Edgar Julián Paredes Gamero,
• Layna Tayná Brito Leite,
• Jessica de Araújo Isaías Muller,
• Mônica Cristina Toffoli Kadri,
• Ariadna Lafourcade Prada and
• Jesús Rafael Rodríguez Amado

Beilstein J. Nanotechnol. 2025, 16, 1277–1288, doi:10.3762/bjnano.16.93

• , nutraceutical, and industrial applications. However, due to the chemical and physicochemical characteristics as well as the solubility and stability of this oil, it was decided to make a nanoemulsion to enhance its already known therapeutic benefits. Nanoemulsions are nanoemulsified systems, either oil-in-water

• ]. Physicochemical characterization of Acrocomia aculeata fruit pulp oil The physicochemical parameters of bocaiúva oil, such as acidity index, iodine index, and refractive index, were analyzed. The acidity index indicates the state of conservation of oils and fats and is related to the oxidation process. Our

• upon formulation with the surfactant system characterized by a hydrophilic–lipophilic balance (HLB) value of 12. These physicochemical parameters are critical for defining the kinetic stability and structural integrity of the nanoemulsion system. Surfactants or emulsifiers are characterized by their

Better together: biomimetic nanomedicines for high performance tumor therapy

• Imran Shair Mohammad,
• Gizem Kursunluoglu,
• Anup Kumar Patel,
• Hafiz Muhammad Ishaq,
• Cansu Umran Tunc,
• Dilek Kanarya,
• Mubashar Rehman,
• Omer Aydin and
• Yin Lifang

Beilstein J. Nanotechnol. 2025, 16, 1246–1276, doi:10.3762/bjnano.16.92

• physicochemical and biological properties, and incredible potential of being therapeutic agent carriers for biomedical applications [10][11]. They are capable to deliver a range of therapeutics including genes, vaccines, biological macromolecules, hydrophobic/hydrophilic drugs, and proteins to certain organs such

Functional bio-packaging enhanced with nanocellulose from rice straw and cinnamon essential oil Pickering emulsion for fruit preservation

• Tuyen B. Ly,
• Duong D. T. Nguyen,
• Hieu D. Nguyen,
• Yen T. H. Nguyen,
• Bup T. A. Bui,
• Kien A. Le and
• Phung K. Le

Beilstein J. Nanotechnol. 2025, 16, 1234–1245, doi:10.3762/bjnano.16.91

• and enhanced food safety [30]. Results and Discussion Physicochemical properties FTIR spectroscopy (Figure 1a) was used to study the PVA/glycerol biopackaging (BP), NC-reinforced BP (rBP), and PE-CEO-containing rBP (rCBP) composite films. BP exhibited characteristic peaks at 3330 cm−1 (O–H stretching

• physicochemical properties of the films. Thermogravimetric analysis (TGA) (Figure 2c) shows how increasing the PE-CEO concentration may affect the thermal stability of the sample. At lower PE-CEO concentration, almost no variation between the rCBP samples can be observed. At 10% (v/w) PE-CEO concentration, a

• cast onto a mold and dried at 70 °C for 12 h. All concentrations were based on the mass of PVA. Characterization Physicochemical properties Fourier transform infrared spectroscopy (FTIR) spectra were recorded in the range of 4000–500 cm−1 using a Bruker ALPHA II spectrometer (Germany) at a spectral

Hydrogels and nanogels: effectiveness in dermal applications

• Jéssica da Cruz Ludwig,
• Diana Fortkamp Grigoletto,
• Daniele Fernanda Renzi,
• Wolf-Rainer Abraham,
• Daniel de Paula and
• Najeh Maissar Khalil

Beilstein J. Nanotechnol. 2025, 16, 1216–1233, doi:10.3762/bjnano.16.90

• - Vila Carli, Guarapuava – PR, Brazil Helmholtz-Zentrum für Infektionsforschung, Inhoffenstrasse 7, 38124 Braunschweig, Germany 10.3762/bjnano.16.90 Abstract Drug delivery systems (DDSs) are an important tool for obtaining medicines with improved physicochemical properties, especially for drugs with

• of methodologies designed to obtain them. In addition to optimizing the physicochemical methods used to prepare nanogels and hydrogels, the application of precision engineering principles has proven essential to enhance the therapeutic performance of these formulations [63][64]. In developing

• thermodynamic activity of the system). Thus, the physicochemical properties related to the first approach involve only the interactions between drugs and vehicles. On the other hand, changing the drug solubility and/or its diffusion through SC is much more complex and comprises multiple interactions among drug

Investigation of the solubility of protoporphyrin IX in aqueous and hydroalcoholic solvent systems

• Michelly de Sá Matsuoka,
• Giovanna Carla Cadini Ruiz,
• Marcos Luciano Bruschi and
• Jéssica Bassi da Silva

Beilstein J. Nanotechnol. 2025, 16, 1209–1215, doi:10.3762/bjnano.16.89

• source, and tissue oxygen. Protoporphyrin IX (PpIX) is commonly used as a PS due to its tumor-targeting properties and phototoxicity. However, the physicochemical properties of PpIX foster self-aggregation, which is a challenge for its incorporation into pharmaceutical formulations. This study aimed to

Chitosan nanocomposite containing rotenoids: an alternative bioinsecticidal approach for the management of Aedes aegypti

• Maria A. A. Bertonceli,
• Vitor D. C. Cristo,
• Ivo J. Vieira,
• Francisco J. A. Lemos,
• Arnoldo R. Façanha,
• Raimundo Braz-Filho,
• Gustavo V. T. Batista,
• Luis G. M. Basso,
• Sérgio H. Seabra,
• Thalya S. R. Nogueira,
• Felipe F. Moreira,
• Arícia L. E. M. Assis,
• Antônia E. A. Oliveira and
• Kátia V. S. Fernandes

Beilstein J. Nanotechnol. 2025, 16, 1197–1208, doi:10.3762/bjnano.16.88

• potential values > ±30 mV are indicative of nanoparticle stability, as strong electrostatic repulsion prevents aggregation and ensures colloidal stability [20][21]. While these measurements provide important physicochemical insights, further evidence of the chemical incorporation and potential interactions

• into the chitosan-based nanocomposite and suggest molecular interactions that may contribute to the enhanced stability and bioactivity of the formulation. To complement the physicochemical characterization and confirm the efficiency of rotenoid encapsulation within the nanoparticles, a calibration

Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

• Sevin Adiguzel,
• Nilay Cicek,
• Zehra Cobandede,
• Feray B. Misirlioglu,
• Hulya Yilmaz and
• Mustafa Culha

Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78

• using a variety of techniques including FTIR, Raman, UV–vis, DLS, and PRFM to confirm their physicochemical properties. Characterization of hexagonal boron nitrides The TEM images of the synthesized hBNs are shown in Supporting Information File 1, Figure S3 (a) and (b). As seen, hBNs have platelet-like

Soft materials nanoarchitectonics: liquid crystals, polymers, gels, biomaterials, and others

• Katsuhiko Ariga

Beilstein J. Nanotechnol. 2025, 16, 1025–1067, doi:10.3762/bjnano.16.77

Serum heat inactivation diminishes ApoE-mediated uptake of D-Lin-MC3-DMA lipid nanoparticles

• Demian van Straten,
• Luuk van de Schepop,
• Rowan Frunt,
• Pieter Vader and
• Raymond M. Schiffelers

Beilstein J. Nanotechnol. 2025, 16, 740–748, doi:10.3762/bjnano.16.57

• proteins, forms on its surface. This so-called protein corona significantly affects the physicochemical properties of the nanoparticle, such as size, charge and stability [2][3][4][5]. In turn, the composition of the protein corona is influenced by the physicochemical properties of the pristine

• supplemented with HI or NHI FCS. Uptake by cells cultured in medium with HI FCS supplemented with 1 µg/mL recombinant ApoE3 was included as positive control. An overview of the physicochemical characteristics of the LNP formulations can be found in Supporting Information File 1 (Figure S1). Three cell lines

• < 0.05 and were annotated as ns = non-significant, * = p ≤ 0.05, ** = p ≤ 0.01, *** = p ≤ 0.001 and **** = p ≤ 0.0001. Supporting Information Supporting Information File 26: Physicochemical characteristics of MC3 and C12 LNPs. Acknowledgements The graphical abstract was generated in Microsoft

Colloidal few layered graphene–tannic acid preserves the biocompatibility of periodontal ligament cells

• Teissir Ben Ammar,
• Naji Kharouf,
• Dominique Vautier,
• Housseinou Ba,
• Nivedita Sudheer,
• Philippe Lavalle and
• Vincent Ball

Beilstein J. Nanotechnol. 2025, 16, 664–677, doi:10.3762/bjnano.16.51

• synthesized through a straightforward, bio-based methodology. Physicochemical characterizations elucidated the structural and morphological attributes of the biocomposite. By incorporating antioxidant TA molecules onto the FLG surface, the biocomposite dynamically mitigated reactive oxygen species

• effective strategy for enhancing their intrinsic antioxidant properties. Herein we report the synthesis of a biocompatible FLG–TA colloidal system with dual functionality. First, we examine whether TA enables the formation of a stable FLG–TA colloid; thus, various physicochemical characterizations were

• Previous findings have shown that TA modifies the physicochemical and structural characteristics of GBMs [33]. The previously observed preservation of metabolic activity in FLG–TA likely stems from two main factors. First, the biocomposite’s ability to protect cells from ROS, which can originate from

Aprepitant-loaded solid lipid nanoparticles: a novel approach to enhance oral bioavailability

• Mazhar Hussain,
• Muhammad Farooq,
• Muhammad Asad Saeed,
• Muhammad Ijaz,
• Sherjeel Adnan,
• Zeeshan Masood,
• Muhammad Waqas,
• Wafa Ishaq and
• Nabeela Ameer

Beilstein J. Nanotechnol. 2025, 16, 652–663, doi:10.3762/bjnano.16.50

• analysis. APT-loaded SLNs were prepared by the precipitation method and characterized by physicochemical studies including particle size and zeta potential measurements, drug content, encapsulation efficiency and solubility studies, Fourier-transform infrared spectroscopy (FTIR), scanning electron

• , or applying cyclodextrin complexation or salt formation. However, these techniques depend on the physicochemical features of the drug in each case. SLNs are the easiest and most suitable scalable of the above approaches for developing stable commercially viable dosage forms [11]. Various methods such

• , and zeta potential measurements. Also Fourier-transform infrared (FTIR) spectroscopy, X-ray diffraction (XRD), solubility, in vitro dissolution, and in vivo and stability studies were carried out. Result and Discussion Physicochemical evaluation The solubility of APT in the SLNs was 24-fold higher

A formulation containing Cymbopogon flexuosus essential oil: improvement of biochemical parameters and oxidative stress in diabetic rats

• Ailton Santos Sena-Júnior,
• Cleverton Nascimento Santana Andrade,
• Pedro Henrique Macedo Moura,
• Jocsã Hémany Cândido dos Santos,
• Cauãn Torres Trancoso,
• Eloia Emanuelly Dias Silva,
• Deise Maria Rego Rodrigues Silva,
• Ênio Pereira Telles,
• Luiz André Santos Silva,
• Isabella Lima Dantas Teles,
• Sara Fernanda Mota de Almeida,
• Daniel Alves de Souza,
• Jileno Ferreira Santos,
• Felipe José Aidar Martins,
• Ana Mara de Oliveira e Silva,
• Sandra Lauton-Santos,
• Guilherme Rodolfo Souza de Araujo,
• Cristiane Bani Correa,
• Rogéria De Souza Nunes,
• Lysandro Pinto Borges and
• Ana Amélia Moreira Lira

Beilstein J. Nanotechnol. 2025, 16, 617–636, doi:10.3762/bjnano.16.48

• and one NTS were also obtained. Among the TLS, point 7 (M7-EOCF) was selected for subsequent tests, as it had the lowest amount of surfactants and the highest proportion of water in its composition. Physicochemical characterization of the selected system Some parameters are fundamental for

• containing 10% EOCF were then prepared individually (Table 1), and the dispersions were evaluated macroscopically and characterized 48 h after they were obtained. Physicochemical characterization of the selected microemulsions The polydispersity index and average hydrodynamic radius of the droplets were

Polyurethane/silk fibroin-based electrospun membranes for wound healing and skin substitute applications

• Iqra Zainab,
• Zohra Naseem,
• Syeda Rubab Batool,
• Muhammad Waqas,
• Ahsan Nazir and
• Muhammad Anwaar Nazeer

Beilstein J. Nanotechnol. 2025, 16, 591–612, doi:10.3762/bjnano.16.46

• antibacterial properties of PU nanofibrous membranes [162]. PU/gelatin and PU/cobalt nitrate were used to create mixed nanofibers scaffolds used to develop wound dressings [162][163]. It was shown that cobalt nitrate added to the PU wound dressing resulted in improved physicochemical properties, blood

Nanomaterials in targeting amyloid-β oligomers: current advances and future directions for Alzheimer's disease diagnosis and therapy

• Shiwani Randhawa,
• Trilok Chand Saini,
• Manik Bathla,
• Rahul Bhardwaj,
• Rubina Dhiman and
• Amitabha Acharya

Beilstein J. Nanotechnol. 2025, 16, 561–580, doi:10.3762/bjnano.16.44

• detection and treatment of AβOs in AD. Because of their nanoscale size, NMs can interact with biological systems in ways that traditional treatments cannot. Their unique properties such as high surface area, quantum effects, and specific physicochemical traits make them ideal for developing advanced

• physicochemical properties, diverse structural forms, and potential applications in combating NDs. The unique characteristics of CNMs, including their hydrophobic surfaces and variable dimensions, enable them to interact effectively with biomolecules, making them valuable tools in biomedical research and

Functionalized gold nanoflowers on carbon screen-printed electrodes: an electrochemical platform for biosensing hemagglutinin protein of influenza A H1N1 virus

• Carlos Enrique Torres-Méndez,
• Sharmilee Nandi,
• Klara Martinovic,
• Patrizia Kühne,
• Yifan Liu,
• Sam Taylor,
• Maria Lysandrou,
• Maria Ines Berrojo Romeyro Mascarenhas,
• Viktoria Langwallner,
• Javier Enrique Sebastián Alonso,
• Ivana Jovanovic,
• Maike Lüftner,
• Georgia-Vasiliki Gkountana,
• David Bern,
• Abdul-Raouf Atif,
• Ehsan Manouchehri Doulabi,
• Gemma Mestres and
• Masood Kamali-Moghaddam

Beilstein J. Nanotechnol. 2025, 16, 540–550, doi:10.3762/bjnano.16.42

• infected patients at an early stage to improve treatment options, recovery time, and economic cost. Biosensors are widely used to detect and quantify different analytes. They incorporate a biorecognition element for detection of the analyte of interest in a sample and a physicochemical transducer to

Synthetic-polymer-assisted antisense oligonucleotide delivery: targeted approaches for precision disease treatment

• Ana Cubillo Alvarez,
• Dylan Maguire and
• Ruairí P. Brannigan

Beilstein J. Nanotechnol. 2025, 16, 435–463, doi:10.3762/bjnano.16.34

• activity of a series of transferrin-conjugated LPNs with varied physicochemical properties, designed to deliver a phosphorothioate ASO targeting ribonucleotide nuclease R1 [114]. Experimental and in vitro studies indicated that optimal biostability and cytocompatibility were achieved with particles bearing

• process of PPI-Mal G4 and PPI-Mal-III G4 dendrimers conjugated with multiple anti-HIV ASOs. Dendriplexes with diverse sizes and morphologies, including 1D rod-like and 3D diamond-shaped structures, were successfully prepared by altering the physicochemical properties of the carriers and the