Serum type and concentration both affect the protein-corona composition of PLGA nanoparticles

• Katrin Partikel,
• Robin Korte,
• Dennis Mulac,
• Hans-Ulrich Humpf and
• Klaus Langer

Beilstein J. Nanotechnol. 2019, 10, 1002–1015, doi:10.3762/bjnano.10.101

• remains stable over a wide range of FBS concentrations, while only the intensity of protein bands evolves until no further increase in staining intensity is visible. These findings reinforce the previously described assertion that the surface of PLGA NPs is more or less fully covered by proteins and a

• , the cells were covered with Vectashield® Mounting medium with DAPI (Vector Laboratories Inc., Burlingname, USA) for nuclear staining. All images were taken using a IX81 fluorescence microscope (Olympus, Hamburg, Germany) with filter systems including excitation at 360–370 nm, dichroic mirror at 400 nm

Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• Figure 4B. Neither Si- nor Au-NPs led to differences in activation or expression of NF-κB. Both forms could be detected for this protein (Figure 4C). Expression of tight-junction proteins in rBCEC4 cells Immunofluorescence staining and TEM were used to demonstrate the expression of important BBB

• -characteristics, namely tight junction (TJ) formation, in rBCEC4 cells. Both, the TJ-associated protein zonula occludens-1 (ZO-1) and the TJ protein occludin, resulted in positive staining (Figure 5A,B). TEM pictures corroborated the formation of TJs between single rBCEC4 cells in a cell monolayer (Figure 5C). A

• possible effect of NP exposure on TJ formation and established TJs was investigated using immunofluorescence staining for ZO-1 (Figure 5D–G). rBCEC4 cells were exposed to PLLA-NPs at a concentration of [24.9 µg/mL] at various time points during and after monolayer and barrier formation. No differences in

Tungsten disulfide-based nanocomposites for photothermal therapy

• Tzuriel Levin,
• Hagit Sade,
• Rina Ben-Shabbat Binyamini,
• Maayan Pour,
• Iftach Nachman and
• Jean-Paul Lellouche

Beilstein J. Nanotechnol. 2019, 10, 811–822, doi:10.3762/bjnano.10.81

• of 157 µm × 157 µm in the middle of each frame was irradiated with a 700 nm laser (Chameleon Vision II) at 123 mW for 1 min. The same frames were then imaged again. A dye exclusion test of cell viability was performed, using Trypan Blue for staining. A mixture of 0.5 wt % trypan blue solution and PBS

Gold nanoparticles embedded in a polymer as a 3D-printable dichroic nanocomposite material

• Lars Kool,
• Anton Bunschoten,
• Aldrik H. Velders and
• Vittorio Saggiomo

Beilstein J. Nanotechnol. 2019, 10, 442–447, doi:10.3762/bjnano.10.43

• , where craftsmen, unaware of the existence of surface plasmon resonance [3], used metallic nanoparticles for coloring mosaic tiles, pottery and glass [4][5]. Metallic nanoparticles were also used for staining glass during medieval times, examples of which can still be found in many churches and

Surface plasmon resonance enhancement of photoluminescence intensity and bioimaging application of gold nanorod@CdSe/ZnS quantum dots

• Siyi Hu,
• Yu Ren,
• Yue Wang,
• Jinhua Li,
• Junle Qu,
• Liwei Liu,
• Hanbin Ma and
• Yuguo Tang

Beilstein J. Nanotechnol. 2019, 10, 22–31, doi:10.3762/bjnano.10.3

• staining appearing in Figure 8 was due to the accumulation of functionalized nanoparticles in the cells, and there was no sign of any damage to the cell, demonstrating passive uptake in MCF-7 breast cancer cells using CdSe/ZnS@FA and GNR@CdSe/ZnS@FA. However, because the PL intensity and cell morphology of

Characterization and influence of hydroxyapatite nanopowders on living cells

• Przemyslaw Oberbek,
• Tomasz Bolek,
• Adrian Chlanda,
• Seishiro Hirano,
• Sylwia Kusnieruk,
• Julia Rogowska-Tylman,
• Ganna Nechyporenko,
• Viktor Zinchenko,
• Wojciech Swieszkowski and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2018, 9, 3079–3094, doi:10.3762/bjnano.9.286

• mitotic division on the left side of both images (scale bar = 10 μm). White arrows point at HAp nanoobjects. c) CLSM image of CHO cells and visible aggregates and agglomerates of F202 (100 µg/mL) hydroxyapatite, and d) image presenting the control CHO cells without any HAp additions. No staining was used

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• morphology of the incubated cells were analysed with calcein acetoxymethyl ester (calcein-AM, Calbiochem, Schwalbach, Germany) fluorescence staining. For this, the cells were incubated with 1 μM calcein-AM for 30 min at 37 °C under cell culture conditions and subsequently analysed by fluorescence microscopy

Size-selected Fe₃O₄–Au hybrid nanoparticles for improved magnetism-based theranostics

• Maria V. Efremova,
• Yulia A. Nalench,
• Eirini Myrovali,
• Anastasiia S. Garanina,
• Ivan S. Grebennikov,
• Polina K. Gifer,
• Maxim A. Abakumov,
• Marina Spasova,
• Makis Angelakeris,
• Alexander G. Savchenko,
• Michael Farle,
• Natalia L. Klyachko,
• Alexander G. Majouga and
• Ulf Wiedwald

Beilstein J. Nanotechnol. 2018, 9, 2684–2699, doi:10.3762/bjnano.9.251

• positive staining of 4T1 cells is found only on the periphery of the cell monolayer. However, ROS activation is observed at this point of time – as indicated by an increased number of H2DCFDA-positive cells in the culture (Figure S5C, Supporting Information File 1). Exposure to AMF for 30 min is sufficient

Cytotoxicity of doxorubicin-conjugated poly[N-(2-hydroxypropyl)methacrylamide]-modified γ-Fe₂O₃ nanoparticles towards human tumor cells

• Zdeněk Plichta,
• Yulia Kozak,
• Rostyslav Panchuk,
• Viktoria Sokolova,
• Matthias Epple,
• Lesya Kobylinska,
• Pavla Jendelová and
• Daniel Horák

Beilstein J. Nanotechnol. 2018, 9, 2533–2545, doi:10.3762/bjnano.9.236

• under Evolution 300 Trino microscope (Delta Optical; Mińsk Mazowiecki, Poland) after cell staining with 0.1% trypan blue. For long-term (72 h) cytotoxicity experiments, MTT assay was used. hMSC, HeLa, and MG-63 cells were plated (5 × 103) in 100 µL of medium per well in 96-well plates and allowed to

• fluorescence (617 nm) after excitation by green light (528 nm). All experiments were carried out in triplicate. Finally, DAPI staining was used for determination of chromatin hypercondensation in the B16 melanoma cells treated with the Dox and its complexes for 24 h. After the treatment with γ-Fe2O3@PHPMA

• (Dox+NP) towards human leukemia cells of K562, Jurkat, HL-60/wt and HL-60/vinc lines (seeded 106 per mL) and murine melanoma cells of B16F10/wt line (seeded 105 per mL); trypan blue staining. Data are relative to the untreated controls and represent the mean +/− SD of three independent experiments. * p

Enhanced antineoplastic/therapeutic efficacy using 5-fluorouracil-loaded calcium phosphate nanoparticles

• Shanid Mohiyuddin,
• Saba Naqvi and
• Gopinath Packirisamy

Beilstein J. Nanotechnol. 2018, 9, 2499–2515, doi:10.3762/bjnano.9.233

• -15) cancer cells at 48.5 μg/mL treatment. The apoptotic induction of CaP@5-FU NPs was confirmed with acridine orange/ethidium bromide (AO/EB) staining and by examining the morphological changes with Hoechst and rhodamine B staining in a time-dependent manner. The apparent membrane bleb formation was

• with dual staining of acridine orange/ethidium bromide (AO/EB) in the cells. The differential staining of the combination AO/EB reveals the different apoptotic phases. AO, with a fluorescence excitation/emission of 431/520 nm, can permeate the cells and yields a bright green fluorescence upon

• excitation. Likewise, EB, having an excitation/emission of 524/605 nm, provides an orange fluorescence upon intercalculation with dsDNA and can permeate into membrane-compromised cells only. The degree of EB staining in the cells dictates the proportion of the cell membrane compromised. During the early

Nanoscale characterization of the temporary adhesive of the sea urchin Paracentrotus lividus

• Ana S. Viana and
• Romana Santos

Beilstein J. Nanotechnol. 2018, 9, 2277–2286, doi:10.3762/bjnano.9.212

• footprints samples were imaged in each condition and representative data were selected. Histochemical staining Binding of the benzothiazole dye thioflavin-T (Sigma-Aldrich) to amyloid structures was detected by direct staining of the adhesive footprints collected on glass slides. Footprints were incubated

• ), moist (b) or under native (c) conditions. The data was obtained either with a ScanAsyst-Air probe (a, b) or a silicon nitride probe (SNL, Bruker) (c). Different coloured lines represent three independent trials. a) Histological staining of the adhesive material deposited by the tube feet of

The structural and chemical basis of temporary adhesion in the sea star Asterina gibbosa

• Birgit Lengerer,
• Marie Bonneel,
• Mathilde Lefevre,
• Elise Hennebert,
• Philippe Leclère,
• Emmanuel Gosselin,
• Peter Ladurner and
• Patrick Flammang

Beilstein J. Nanotechnol. 2018, 9, 2071–2086, doi:10.3762/bjnano.9.196

• homogenous granules. The footprints comprised a meshwork on top of a thin layer. This topography was consistently observed using various methods like scanning electron microscopy, 3D confocal interference microscopy, atomic force microscopy, and light microscopy with crystal violet staining. Additionally, we

• results, indicating the intensity of staining on the different tissues is given in Table 1. Details on the sugar moieties recognized by the lectins are listed in (Supporting Information File 1, Table S1). Out of the 24 tested lectins, 15 led to a labelling in the disc epidermis (Figure 6, Supporting

• Information File 1, Figure S2,S3). Concanavalin A (Con A) strongly reacted with most tissues of the tube feet, except the connective tissue (Figure 6A1). In the disc epidermis, the area of the gland cells was strongly labelled from the gland cell bodies to the secretory pores (Figure 6A2). This staining

Biomimetic and biodegradable cellulose acetate scaffolds loaded with dexamethasone for bone implants

• Aikaterini-Rafailia Tsiapla,
• Varvara Karagkiozaki,
• Veroniki Bakola,
• Foteini Pappa,
• Panagiota Gkertsiou,
• Eleni Pavlidou and
• Stergios Logothetidis

Beilstein J. Nanotechnol. 2018, 9, 1986–1994, doi:10.3762/bjnano.9.189

• . Cytotoxicity studies were performed by using MTT assay, methylene-blue staining and SEM fixation and showed very good cell adhesion and proliferation, indicating the cytocompatibility of these fibrous scaffolds. Drug-release kinetics was measured for the evaluation of a controllable and sustained release of

• staining: L929 mouse fibroblasts were used to examine the cytotoxicity levels due to their properties and biological characteristics (biological responses and reproducible growth rates). The samples were placed inside a well-plate and 1 mL of medium was added and the whole system was left in the incubator

• of PBS was added. Methylene blue is commonly used for the identification of cell viability as it stains the nuclei of living cells, making them more observable. The protocol for staining with methylene blue initially involves the addition of methanol to the samples, which were in the well-plates for

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

• Reika Makita,
• Tsukasa Akasaka,
• Seiichi Tamagawa,
• Yasuhiro Yoshida,
• Saori Miyata,
• Hirofumi Miyaji and
• Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

• patterns The cell viability of Saos-2 cells on the gelatin crosslinked with 20 mM genipin was easily estimated with live/dead double staining (Figure 4). The gelatin pillars, with diameters of 500 nm and heights of 500 nm, were used as gelatin patterns. The high ratio of green-stained cells on gelatin

• with a width or diameters of 500 nm. Immunofluorescence staining after 1 day of culturing Immunofluorescence images of Saos-2 cells on the gelatin patterns are shown in Figure 6. Vinculin (green), F-actin (red), and nuclei (blue) of the cells were stained after 1 day of culturing. The cells were

• . Immunofluorescence staining after 7 days of culturing Immunofluorescence images of Saos-2 cells on the gelatin patterns after 7 days of culturing are shown in Figure 9. The images of vinculin (green), F-actin (red), and nuclei (blue) were quite similar to the images obtained after 1 day of culturing (Figure 6

Humidity-dependent wound sealing in succulent leaves of Delosperma cooperi – An adaptation to seasonal drought stress

• Olga Speck,
• Mark Schlechtendahl,
• Florian Borm,
• Tim Kampowski and
• Thomas Speck

Beilstein J. Nanotechnol. 2018, 9, 175–186, doi:10.3762/bjnano.9.20

• (Technical Workshop, Institute of Biology II/III, University of Freiburg, Germany) was used to cut thin sections between 5 µm and 10 µm in thickness. Overview staining with toluidine blue (0.05% toluidine blue in distilled water) was used to give contrast to the various leaf tissues. Unlignified primary cell

Co-reductive fabrication of carbon nanodots with high quantum yield for bioimaging of bacteria

• Jiajun Wang,
• Xia Liu,
• Gesmi Milcovich,
• Tzu-Yu Chen,
• Edel Durack,
• Sarah Mallen,
• Yongming Ruan,
• Xuexiang Weng and
• Sarah P. Hudson

Beilstein J. Nanotechnol. 2018, 9, 137–145, doi:10.3762/bjnano.9.16

• . Currently, visual detection approaches are based on indirect methods related to bacterially secreted metabolites or imaging of bacterial colonies [4]. Furthermore, the staining techniques use either commercially available fluorescent dyes or semiconductor quantum dots [5]. Fluorescent dyes are expensive

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• liquid nitrogen. Analyses of in-tissue doxorubicin penetration depth (i.e., distance between luminal surface and innermost positive staining for doxorubicin accumulation) were performed by confocal laser microscopy (Leica TCS SP8, Leica Microsystems GmbH, Wetzlar, Germany) with immersion oil on ten

• charging. As expected, the even more homogeneously deposited ultrafine droplets of the extracavitary-charged HINAT-LAU aerosol showed neither a local methylene blue staining nor a visible staining of the whole peritoneum. In the case of intracavitary aerosol change, which goes along with an increased

• droplet deposition based on the formation of an electrical field between electrode and grounded peritoneum, a significant dye staining could be observed near the electrode as shown in Figure 10. In contrast to the extracavitary-charged HINAT-LAU aerosol, PIPAC-MIP operation with aqueous methylene blue

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

• Dimitri Vanhecke,
• Dagmar A. Kuhn,
• Dorleta Jimenez de Aberasturi,
• Sandor Balog,
• Ana Milosevic,
• Dominic Urban,
• Diana Peckys,
• Niels de Jonge,
• Wolfgang J. Parak,
• Alke Petri-Fink and
• Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

• × phosphate-buffered saline (PBS). After 1 h of staining (in the dark at RT) and three washing cycles with 1× PBS the cells were mounted using Glycergel mounting media (C0563, Dako, Baar, Switzerland). For live-cell imaging, cells were seeded in a Lab-TekTM II chambered cover glass four-well chamber (1.5

• in 0.15 M cacodylate buffer. Staining occurred with osmium tetraoxide in ddH2O. After the staining, membranes were transferred to 0.05 M maleate buffer and dehydrated with an increased ethanol series (30% and 70%). The membranes were embedded in epon and polymerized at 60 °C. The samples were

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging

• Joseph R. Pyle and
• Jixin Chen

Beilstein J. Nanotechnol. 2017, 8, 2296–2306, doi:10.3762/bjnano.8.229

• homogeneous staining as described by Carlsson et al. [42]. Approximately 200 µL of the YOYO–DNA solution was injected into the microfluidic channel using a syringe pump (New Era Pump Systems Inc., model NE-1000) at 0.40 mL/min. YOYO–DNA adhered to the surface of the amine-modified glass through electrostatic

• fluorescence lifetime of YOYO-1 in DNA is 2–5 ns [52], that is, much shorter than the photon flux interval, all laser powers studied in this work should not be high enough for two-photon absorption to occur. Thus, the error in the experiment is due to both inhomogeneous dye staining and DNA stretching quality

Fabrication of carbon nanospheres by the pyrolysis of polyacrylonitrile–poly(methyl methacrylate) core–shell composite nanoparticles

• Dafu Wei,
• Youwei Zhang and
• Jinping Fu

Beilstein J. Nanotechnol. 2017, 8, 1897–1908, doi:10.3762/bjnano.8.190

• solution under stirring. After about 20 h of stirring, the mixture was then used for the preparation of samples for TEM observation. The dried copper containing the above NaSCN-treated nanoparticles was further treated with gentle water washing to remove NaSCN salt and negative staining with the PTA

• adjusting the feeding monomer ratio. TEM images The TEM micrographs of the PAN nanoparticles and the two PAN–PMMA nanoparticles are displayed in Figure 4. As disclosed by Figure 4a, the staining agent phosphate tungsic acid (PTA) molecules could diffuse into and interact with the PAN nanoparticles. Thus

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• solutions. A) Confocal images of MDA-MB-231 cells after 24 h treatment with Tween 80-coated core–shell UCNPs (10 µg/mL); UCNPs are green, DAPI staining is blue, the red color represents excitation scattering from intracellular structures. Scale bar equals 10 µm. B) Viability of MCF-7 and MDA-MB-213 cells

Collembola cuticles and the three-phase line tension

• Håkon Gundersen,
• Hans Petter Leinaas and
• Christian Thaulow

Beilstein J. Nanotechnol. 2017, 8, 1714–1722, doi:10.3762/bjnano.8.172

• al demonstrated a lipid layer (epicuticular wax) covering all parts of the Collembola cuticle, using time-of-flight secondary ion mass spectrometry [30]. A lipophilic dye, such as Nile Red, will bind to any part of such a layer it came into contact with, thus staining the part of a surface wetted by

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• 10 min intervals. Viability of SAOS-2 cells incubated with HPHT NDs for three concentrations as a function of the mean particle diameter after 3 days. (Upper row) results of an MTS assay, (lower row) results of cell counting after cell staining. The results are given as the mean ± SD from 3

• row) results of the MTS assay; (lower row) results of cell counting after cell staining. The results are given as the mean ± SD from 3 experiments, each performed in sextuplicate. ANOVA, Tukey HSD post-hoc test. “*” indicates a significant difference from MR-18 at a concentration of 1000 µg/mL (p

• < 0.05). Viability of SAOS-2 cells incubated with NDs at three concentrations as a function of ND type and surface treatment after 3 days. (Upper row) results of the MTS assay; (lower row) results of cell counting after nuclei staining. The results are given as the mean ± SD from 3 experiments, each

A nanocomplex of C₆₀ fullerene with cisplatin: design, characterization and toxicity

• Svitlana Prylutska,
• Svitlana Politenkova,
• Kateryna Afanasieva,
• Volodymyr Korolovych,
• Kateryna Bogutska,
• Andriy Sivolob,
• Larysa Skivka,
• Maxim Evstigneev,
• Viktor Kostjukov,
• Yuriy Prylutskyy and
• Uwe Ritter

Beilstein J. Nanotechnol. 2017, 8, 1494–1501, doi:10.3762/bjnano.8.149

• compounds. The genotoxicity of С60 fullerene, Cis and their complex was evaluated in vitro with the comet assay using human resting lymphocytes and lymphocytes after blast transformation. The cytotoxicity of the mentioned compounds was estimated by Annexin V/PI double staining followed by flow cytometry

• determined using the image analysis software programs Comet Assay IV (Perspective Instruments, UK) and CometScore (TriTec Corp., USA). Cell-death assay Apoptosis was assessed by staining cells with Annexin V–fluorescein isothiocyanate (FITC) and counterstaining with propidium iodide (PI) with the use Annexin

• fullerene can either consume ROS or induce their generation [59]. Taking into account this fact we have hypothesized that C60 fullerene in the nanocomplex with Cis can affect mode of cell death induced by Cis. In order to testify this hypothesis, Annexin V/PI double staining of human healthy lymphocytes