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Search for "cell viability" in Full Text gives 169 result(s) in Beilstein Journal of Nanotechnology.

Preparation of micro/nanopatterned gelatins crosslinked with genipin for biocompatible dental implants

  • Reika Makita,
  • Tsukasa Akasaka,
  • Seiichi Tamagawa,
  • Yasuhiro Yoshida,
  • Saori Miyata,
  • Hirofumi Miyaji and
  • Tsutomu Sugaya

Beilstein J. Nanotechnol. 2018, 9, 1735–1754, doi:10.3762/bjnano.9.165

Graphical Abstract
  • change was observed at the highest concentration of genipin (20 mM). Because of its high stability in cell culture medium, we used gelatin patterned using 20 mM genipin as a crosslinker to subsequently examine cell attachment and proliferation. Live/dead cell viability assay of Saos-2 cells on gelatin
  • patterns The cell viability of Saos-2 cells on the gelatin crosslinked with 20 mM genipin was easily estimated with live/dead double staining (Figure 4). The gelatin pillars, with diameters of 500 nm and heights of 500 nm, were used as gelatin patterns. The high ratio of green-stained cells on gelatin
  • attachment. Moreover, the live/dead cell viability assay demonstrated that gelatin crosslinked with genipin showed low cytotoxicity (Figure 4). The low cytotoxicity for Saos-2 cells on our gelatin patterns was in agreement with the low cytotoxicity for fibroblasts on genipin-crosslinked gelatin [66
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Published 11 Jun 2018

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

  • Nagamalai Vasimalai,
  • Vânia Vilas-Boas,
  • Juan Gallo,
  • María de Fátima Cerqueira,
  • Mario Menéndez-Miranda,
  • José Manuel Costa-Fernández,
  • Lorena Diéguez,
  • Begoña Espiña and
  • María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

Graphical Abstract
  • -dots in the cancer cultures compared to the non-cancerous cells. Results showed that the spice-derived C-dots inhibited cell viability dose-dependently after a 24 h incubation period, displaying a higher toxicity in LN-229, than in HK-2 cells. As a control, C-dots synthesized from citric acid did not
  • yield among all of the spice-derived C-dots. A summary of the characteristic parameters studied for each spice C-dot is collected in Table 1. Cell viability measurements and cell imaging using the C-dots Concentrations varying from 0.1 mg·mL−1 to 2 mg·mL−1 (and up to 4 mg·mL−1 in the case of black
  • during the in vitro cell viability studies match very well with those assayed in previous works using other types of carbon dots [27]. The highest tested C-dot concentrations correspond to approximately 15% of water; in all cases, cell death due to the water vehicle was excluded by testing cell viability
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Published 13 Feb 2018

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

  • Liga Saulite,
  • Karlis Pleiko,
  • Ineta Popena,
  • Dominyka Dapkute,
  • Ricardas Rotomskis and
  • Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

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  • -dependent endocytosis, whereas the clathrin/caveolae-dependent pathway dominated in MDA-MB-231 cells in monocultures (Supporting Information File 1, Figure S2). Cell viability in 3D culture Cells in a 3D culture formed floating and dense spheroids. Therefore, we sought to analyse the effect of the 3D
  • culture conditions on cell viability (Figure 5). MSC and breast cancer cell populations were distinguished by CD90 expression, thus allowing viability estimations in each cell type separately. Cell viability in 2D culture was greater than 95% (data not shown). MSCs cultivated in 3D monocultures were fully
  • viable after 24 h; nevertheless, a distinct decrease in viability of 26% was observed after 48 h (Figure 5A). MCF7 and MDA-MB-231 cell viability was not changed after 24 h. However, after 48 h, the viability of MCF7 cells was reduced by 31% (Figure 5A). The viability of MDA-MB-231 cells remained
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Published 29 Jan 2018

Co-reductive fabrication of carbon nanodots with high quantum yield for bioimaging of bacteria

  • Jiajun Wang,
  • Xia Liu,
  • Gesmi Milcovich,
  • Tzu-Yu Chen,
  • Edel Durack,
  • Sarah Mallen,
  • Yongming Ruan,
  • Xuexiang Weng and
  • Sarah P. Hudson

Beilstein J. Nanotechnol. 2018, 9, 137–145, doi:10.3762/bjnano.9.16

Graphical Abstract
  • . Different concentrations of C-dots (0, 2.5, 5.0, 10 and 20 μg mL−1) were added into Erlenmeyer flasks and shaken for 2 min at 180 rpm. Furthermore, 0.2 mL of culture broth was collected at different time points (0–72 h) and their optical density was measured at 600 nm in order to calculate the cell
  • viability. Quantification is reported as relative values to the negative control, where the negative control (untreated) is set to 100% viability. TEM and HRTEM (inset) images of (A) Sa, (B) Sb, (C) Se samples, and corresponding size (diameter) distribution ranges for (D) Sa, (E) Sb, and (F) Se. (A–C) UV
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Published 12 Jan 2018

Involvement of two uptake mechanisms of gold and iron oxide nanoparticles in a co-exposure scenario using mouse macrophages

  • Dimitri Vanhecke,
  • Dagmar A. Kuhn,
  • Dorleta Jimenez de Aberasturi,
  • Sandor Balog,
  • Ana Milosevic,
  • Dominic Urban,
  • Diana Peckys,
  • Niels de Jonge,
  • Wolfgang J. Parak,
  • Alke Petri-Fink and
  • Barbara Rothen-Rutishauser

Beilstein J. Nanotechnol. 2017, 8, 2396–2409, doi:10.3762/bjnano.8.239

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  • environment and if so, on which time scale. None of the exposure conditions impaired the cell viability of J774A.1 after 24 h as shown by the LDH and trypan blue exclusion assays (Figure S15 and Figure S16, Supporting Information File 1). Live-cell LSM data revealed that both particle types appeared
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Published 14 Nov 2017

Evaluating the toxicity of TiO2-based nanoparticles to Chinese hamster ovary cells and Escherichia coli: a complementary experimental and computational approach

  • Alicja Mikolajczyk,
  • Natalia Sizochenko,
  • Ewa Mulkiewicz,
  • Anna Malankowska,
  • Michal Nischk,
  • Przemyslaw Jurczak,
  • Seishiro Hirano,
  • Grzegorz Nowaczyk,
  • Adriana Zaleska-Medynska,
  • Jerzy Leszczynski,
  • Agnieszka Gajewicz and
  • Tomasz Puzyn

Beilstein J. Nanotechnol. 2017, 8, 2171–2180, doi:10.3762/bjnano.8.216

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  • preliminary experiment. CHO-K1 proved to be the most sensitive, allowed for the determination of EC50 values for all tested nanomaterials and therefore was selected for the main experiment. A colorimetric assay with WST-8 reagent was used for the cell viability tests: CHO-K1 cells were pre-cultured in F12
  • . Because Au nanoparticles absorb light in the visible region, the plates were centrifuged to avoid interference with the assay. At the next step, 100 µL of medium from each cell culture was transferred to a 96-well plate and the absorbance at 450 nm was measured. Cell viability was calculated as means of
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Published 17 Oct 2017

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

  • Stefania Lettieri,
  • Marta d’Amora,
  • Adalberto Camisasca,
  • Alberto Diaspro and
  • Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

Graphical Abstract
  • treated with only cell culture medium were used as a control. The cell viability percentage was above 80%, showing that CNOs exhibited moderate toxicity to the cells at the tested concentrations (Figure 6). The observed high viability of the HeLa cells treated with CNOs demonstrated their suitability for
  • a positive control for cytotoxicity, the cells were incubated with 5% DMSO. Cell viability was determined using the cell proliferation reagent WST-1 (Roche Applied Sciences). After aspiration of the culture medium, a mixture of DMEM and WST1 reagent (1/10 volume) was added to each well. After 2 h of
  • µg mL−1. Cellular viability of HeLa cells treated with different concentrations (1, 2, 5, 10 and 20 µg mL−1) of fluo-CNOs for 12, 24, 28 and 72 h in DMEM (pH 7.4), revealed by the WST 1 assay. Cell viability (%) was evaluated for the CNO-treated samples against a non-treated control. As a positive
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Published 07 Sep 2017

Synthesis and functionalization of NaGdF4:Yb,Er@NaGdF4 core–shell nanoparticles for possible application as multimodal contrast agents

  • Dovile Baziulyte-Paulaviciene,
  • Vitalijus Karabanovas,
  • Marius Stasys,
  • Greta Jarockyte,
  • Vilius Poderys,
  • Simas Sakirzanovas and
  • Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

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  • . The similar localization of Tween 80-coated nanoparticles was observed in MCF-7 cells as well. The same results of endocytic NP accumulation in cells was demonstrated in different studies with UCNPs [31], quantum dots [32], magnetic nanomaterials [33] and noble metal nanoparticles [34]. Cell viability
  • detectors from reflected and scattered NIR light. Cell viability assay: MCF-7 and MDA-MB-231 human breast cancer cells were seeded on a 96-well plate at a density of 20,000 cells/well. After 24 h, the old medium was replaced with a fresh medium containing 5, 10, 20, 50 and 100 µg/mL core–shell UCNPs. 12
  • , treated with different concentrations of UCNPs for 24 h. Toxicity of UCNPs was investigated using XTT cell viability assay. Formation of water-soluble core and core–shell UCNPs by coating with Tween 80. Supporting Information Supporting Information File 134: The hydrodynamic particle size and zeta
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Published 01 Sep 2017

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

  • Antonín Brož,
  • Lucie Bačáková,
  • Pavla Štenclová,
  • Alexander Kromka and
  • Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

Graphical Abstract
  • 5 nm, H-terminated), and the same detonation NDs further oxidized by annealing at 450 °C. The influence of the NDs on cell viability and cell count was measured by the mitochondrial metabolic activity test and by counting cells with stained nuclei. The interaction of NDs with cells was monitored by
  • phase contrast live-cell imaging in real time. For both types of oxygen-terminated HPHT NDs, the cell viability and the cell number remained almost the same for concentrations up to 100 µg/mL within the whole range of ND diameters tested. The uptake of hydrogen-terminated detonation NDs caused the
  • types. Keywords: cell viability; FTIR; live-cell imaging; MTS; nanodiamond; SAOS-2 cells; Introduction Carbon-based materials in the form of nanostructures are showing great promise as engineering and biomedical materials [1]. Moreover, diamond represents a new class of material with properties that
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Published 10 Aug 2017

Calcium fluoride based multifunctional nanoparticles for multimodal imaging

  • Marion Straßer,
  • Joachim H. X. Schrauth,
  • Sofia Dembski,
  • Daniel Haddad,
  • Bernd Ahrens,
  • Stefan Schweizer,
  • Bastian Christ,
  • Alevtina Cubukova,
  • Marco Metzger,
  • Heike Walles,
  • Peter M. Jakob and
  • Gerhard Sextl

Beilstein J. Nanotechnol. 2017, 8, 1484–1493, doi:10.3762/bjnano.8.148

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  • for all batches show a decrease of the relaxivity of about 11.6% after nine months. Finally, the cell viability of the NPs stabilized with Melpers®2450 was evaluated in hdF and we can show that the NP system is biocompatible and non-toxic. Overall, we have developed a very promising particle system
  • cell toxicity of the CaF2:(Tb3+,Gd3+) NPs was investigated in 96-well plates on a subconfluent monolayer culture of hdF. With the CellTiter-Glo luminescent cell viability assay (Promega), based on the quantification of the ATP concentration, the cell viability was examined. The cell line was seeded
  • -containing cell-culture medium and b) absorbance measurement (λabs = 700 nm) of the samples over a period of 24 h. a) Representative microscopic image of hdF 24 h after treatment with the NPs (c = 1 mg·mL−1). b) Cell viability 24 h after adding CaF2:(Tb3+,Gd3+) NPs at concentrations between 0.5 and 1 mg·mL−1
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Published 18 Jul 2017

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

  • Gamze Varan,
  • Juan M. Benito,
  • Carmen Ortiz Mellet and
  • Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

Graphical Abstract
  • assay. This cell line is recommended by the U.S. Pharmacopeial Convention (USP) for the cytotoxicity evaluation of polymeric systems and was therefore used. According to MTT assay, cell viability for L929 cells is given in Figure 6. It is clearly shown that all blank amphiphilic CD nanoparticle
  • -loaded nanoparticles was determined on MCF-7 cell lines. After an incubation period, cell viability was calculated, as shown in Figure 8. According to the results of anticancer activity studies on MCF-7, PCX-loaded amphiphilic CD nanoparticles have higher cytotoxicity than PCX solution in DMSO (p < 0.05
  • ). The amphiphilic CD nanoparticles and the drug solution carry an equivalent amount of PCX (250 nM) during the cell culture study. The cell viability in loaded CD nanoparticles is significantly different from the PCX solution (p < 0.05). Moreover, the effect of surface charge on viability of cancer
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Published 13 Jul 2017

Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

  • Cem Varan and
  • Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

Graphical Abstract
  • assay, cell viability for L929 cells is given in Figure 6 for 24 h and 48 h. When compared with the control group, the blank formulations were found to have no cytotoxic effect on L929 fibroblast cells (the differences between groups were statistically insignificant, p > 0.05), and it can be suggested
  • . Then, DMEM was replaced with fresh medium containing blank nanoparticle formulations and incubated for 48 h. MTT assay was applied to determine cell viability. 20 µL of MTT solution in PBS (5 mg/mL) were added in each well and incubated for 4 h. 80 µL of MTT lysis solution containing SDS (23% w/v) and
  • DMF (45% v/v) in ultrapure water were added in plates and incubated overnight. The optical density (OD) was determined by a microplate reader (Molecular Devices, USA) at 450 nm (n = 3). The results were expressed in terms of cell viability (%) according to the equation: Statistical analysis All
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Published 12 Jul 2017

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

  • Liga Saulite,
  • Dominyka Dapkute,
  • Karlis Pleiko,
  • Ineta Popena,
  • Simona Steponkiene,
  • Ricardas Rotomskis and
  • Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

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  • quantum dots QD655 were studied in human skin MSCs. The effect of QD on MSCs was examined using a cell viability assay, Ki67 expression analysis, and tri-lineage differentiation assay. The optimal conditions for QD uptake in MSCs were determined using flow cytometry. The QD uptake route in MSCs was
  • in skin MSCs was clathrin-mediated endocytosis. QDs were mainly localized in early endosomes after 6 h as well as in late endosomes and lysosomes after 24 h. QDs in concentrations ranging from 0.5 to 64 nM had no effect on cell viability and proliferation. The expression of MSC markers, CD73 and CD90
  • , comparing unlabelled and labelled cell populations. Cell-viability assay The impact of carboxyl-coated QD655 on the viability of MSCs was analysed using the Cell Counting Kit 8 (CCK-8) (Sigma-Aldrich, USA). A total of 5 × 103 cells per well were seeded onto 96-well plates in 100 μL of complete medium. The
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Published 07 Jun 2017

Silicon microgrooves for contact guidance of human aortic endothelial cells

  • Sara Fernández-Castillejo,
  • Pilar Formentín,
  • Úrsula Catalán,
  • Josep Pallarès,
  • Lluís F. Marsal and
  • Rosa Solà

Beilstein J. Nanotechnol. 2017, 8, 675–681, doi:10.3762/bjnano.8.72

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  • , Spain) with an atmosphere containing 5% CO2. Cell viability and cytotoxicity Cell viability was assessed by morphology using phase-contrast microscopy and by trypan blue dye exclusion test (Merck). Viability 97% was required for the thawed HAECs in order to guarantee the viability of the cells before
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Published 22 Mar 2017

Facile fabrication of luminescent organic dots by thermolysis of citric acid in urea melt, and their use for cell staining and polyelectrolyte microcapsule labelling

  • Nadezhda M. Zholobak,
  • Anton L. Popov,
  • Alexander B. Shcherbakov,
  • Nelly R. Popova,
  • Mykhailo M. Guzyk,
  • Valeriy P. Antonovich,
  • Alla V. Yegorova,
  • Yuliya V. Scrypynets,
  • Inna I. Leonenko,
  • Alexander Ye. Baranchikov and
  • Vladimir K. Ivanov

Beilstein J. Nanotechnol. 2016, 7, 1905–1917, doi:10.3762/bjnano.7.182

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  • effect might have been due to the passivation of the particle surface by DMSO molecules [48]. Biological properties of O-dots Cellular toxicity The results of studying the effect of O-dots samples on cell viability and metabolic activity of NADP-H-dependent oxidoreductases are shown in Figure 4 (for more
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Published 02 Dec 2016

Low temperature co-fired ceramic packaging of CMOS capacitive sensor chip towards cell viability monitoring

  • Niina Halonen,
  • Joni Kilpijärvi,
  • Maciej Sobocinski,
  • Timir Datta-Chaudhuri,
  • Antti Hassinen,
  • Someshekar B. Prakash,
  • Peter Möller,
  • Pamela Abshire,
  • Sakari Kellokumpu and
  • Anita Lloyd Spetz

Beilstein J. Nanotechnol. 2016, 7, 1871–1877, doi:10.3762/bjnano.7.179

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  • Organization, Intel Corporation, Hillsboro, USA Division of Applied Sensor Science, Department of Physics, Chemistry and Biology, Linköping University, SE-58183 Linköping, Sweden 10.3762/bjnano.7.179 Abstract Cell viability monitoring is an important part of biosafety evaluation for the detection of toxic
  • effects on cells caused by nanomaterials, preferably by label-free, noninvasive, fast, and cost effective methods. These requirements can be met by monitoring cell viability with a capacitance-sensing integrated circuit (IC) microchip. The capacitance provides a measurement of the surface attachment of
  • medium above the capacitors. Moreover, the manufacturing of microfluidic channels in the LTCC package was demonstrated. Keywords: capacitance sensing; cell viability; lab-on-a-chip; low temperature co-fired ceramic (LTCC); Introduction Biosafety regulations require ethical, simple, rapid, and cost
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Published 29 Nov 2016

Antitumor magnetic hyperthermia induced by RGD-functionalized Fe3O4 nanoparticles, in an experimental model of colorectal liver metastases

  • Oihane K. Arriortua,
  • Eneko Garaio,
  • Borja Herrero de la Parte,
  • Maite Insausti,
  • Luis Lezama,
  • Fernando Plazaola,
  • Jose Angel García,
  • Jesús M. Aizpurua,
  • Maialen Sagartzazu,
  • Mireia Irazola,
  • Nestor Etxebarria,
  • Ignacio García-Alonso,
  • Alberto Saiz-López and
  • José Javier Echevarria-Uraga

Beilstein J. Nanotechnol. 2016, 7, 1532–1542, doi:10.3762/bjnano.7.147

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  • could seriously compromise tumor cell viability. For such tumor destruction it is necessary to combine the heat capacity of the magnetic system with its localization in tumor tissues. However, the creation of high quality magnetic nanosystems that meet both requirements is still a great challenge
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Published 28 Oct 2016

On the pathway of cellular uptake: new insight into the interaction between the cell membrane and very small nanoparticles

  • Claudia Messerschmidt,
  • Daniel Hofmann,
  • Anja Kroeger,
  • Katharina Landfester,
  • Volker Mailänder and
  • Ingo Lieberwirth

Beilstein J. Nanotechnol. 2016, 7, 1296–1311, doi:10.3762/bjnano.7.121

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  • . Similar results were reported by Zhang et al. comparing 80 nm SiNPs with 500 nm SiNPs on HepG2 cells [40]. Those investigated particles affect cell viability and the proliferation potential in a size-ascending-dependent manner. Nevertheless, these data were only focusing on large differences in size and
  • surface-area dependent cytotoxicity and focused on long incubation times of SiNPs in the order of some 24 h. In our experiments we applied high concentrations of SiNPs and could observe effects on cell viability and membrane integrity already after a few hours. When inspecting the LDH release experiments
  • the following formula (Abs: Absorbance): Measurement of intracellular ATP content Intracellular ATP content was measured according to the kit instructions of CellTiter-Glo® Luminescent Cell Viability Assay (Promega, U.S.A.). Briefly, 15,000 HeLa cells·cm−2 were seeded on a 96-well plate. After
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Published 16 Sep 2016

Straightforward and robust synthesis of monodisperse surface-functionalized gold nanoclusters

  • Silvia Varela-Aramburu,
  • Richard Wirth,
  • Chian-Hui Lai,
  • Guillermo Orts-Gil and
  • Peter H. Seeberger

Beilstein J. Nanotechnol. 2016, 7, 1278–1283, doi:10.3762/bjnano.7.118

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  • for one day with the mouse cell line L929 for a proof-of-principle study. Cell viability was measured using the MTS [3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium inner salt] assay [30]. The cytotoxicity of Glc-NCs, CTAB-NCs and THPC-NCs was compared. CTAB
  • lectin ConA (4). Cell viability of Glc-NCs A) purified and B) without purification incubated for one day with L929 cells. The Glc-NCs were not toxic at any of the concentrations studied. C) Cellular uptake of Glc-NCs when incubated with L929 cells for one day. Gold concentration taken up by the cells was
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Published 08 Sep 2016

Reasons and remedies for the agglomeration of multilayered graphene and carbon nanotubes in polymers

  • Rasheed Atif and
  • Fawad Inam

Beilstein J. Nanotechnol. 2016, 7, 1174–1196, doi:10.3762/bjnano.7.109

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  • , as well as to different methods to measure cell viability and different CNT sources. More efforts are needed to solve these issues prior to the incorporation of MLG/CNT–polymer nanocomposites into the human body. Therefore, it is a prerequisite to master the production of MLG- and CNT-based polymer
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Published 12 Aug 2016

Multiwalled carbon nanotube hybrids as MRI contrast agents

  • Nikodem Kuźnik and
  • Mateusz M. Tomczyk

Beilstein J. Nanotechnol. 2016, 7, 1086–1103, doi:10.3762/bjnano.7.102

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  • the in vivo behavior of nanohybrids is essential in order to judge their applicability in MRI. Several types of studies were performed. The first group was to study cytotoxicity by determining cell viability upon incubation with the nanohybrids (Table 2). In some cases it was possible to indicate
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Review
Published 27 Jul 2016

Improved biocompatibility and efficient labeling of neural stem cells with poly(L-lysine)-coated maghemite nanoparticles

  • Igor M. Pongrac,
  • Marina Dobrivojević,
  • Lada Brkić Ahmed,
  • Michal Babič,
  • Miroslav Šlouf,
  • Daniel Horák and
  • Srećko Gajović

Beilstein J. Nanotechnol. 2016, 7, 926–936, doi:10.3762/bjnano.7.84

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  • counterstained with 0.1% Nuclear Fast Red (Sigma-Aldrich) for 1 min, mounted with HistoMount (Invitrogen) and covered using coverslip. After drying, the cells were analyzed under bright field using light microscope (ECLIPSE E200, Nikon Instruments, Japan). MTT cell viability assay After NSC labeling MTT (methyl
  • labeled with different concentrations of PLL-γ-Fe2O3 (A) and nanomag®-D-spio (B) nanoparticles (N = 5). The asterisk indicates a statistically significant difference (P < 0.05) versus other concentrations of the same nanoparticle. PLL-γ-Fe2O3 nanoparticles did not affect NSC proliferation. MTT cell
  • viability assay of NSCs labeled with PLL-γ-Fe2O3 and nanomag®-D-spio nanoparticles (N = 12). The statistically significant diferences versus Control were depicted by asterisks, *: P < 0.05; **: P < 0.005; ***: P < 0.001. PLL-γ-Fe2O3 nanoparticles had low NSC cytotoxicity. Flow cytometry analysis showed the
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Published 27 Jun 2016

Tight junction between endothelial cells: the interaction between nanoparticles and blood vessels

  • Yue Zhang and
  • Wan-Xi Yang

Beilstein J. Nanotechnol. 2016, 7, 675–684, doi:10.3762/bjnano.7.60

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  • induce brain dysfunction and pathology [25] and in some cases have an impact on gene expression in neural cells [26]. CuO NPs reduce cell viability and also cause oxidative stress in human bronchial epithelial cells [27]. Interaction between NPs and blood circulatory system The circulatory system or
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Review
Published 06 May 2016

Unraveling the neurotoxicity of titanium dioxide nanoparticles: focusing on molecular mechanisms

  • Bin Song,
  • Yanli Zhang,
  • Jia Liu,
  • Xiaoli Feng,
  • Ting Zhou and
  • Longquan Shao

Beilstein J. Nanotechnol. 2016, 7, 645–654, doi:10.3762/bjnano.7.57

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  • -2 levels, indicated that mitochondria- and endoplasmic reticulum-mediated signaling pathways were involved in the apoptotic process. TiO2 NPs were also shown to decrease cell viability by inducing apoptosis in the microglia N9 [36] and human astrocytes-like astrocytoma U87 cell lines [37]. Direct
  • toxic effects on cell structures Cell components, such as the cell membrane and mitochondria, can be targets of TiO2 NPs. TiO2 NPs can decrease cell viability of primary rat astrocytes. Herein, the mitochondrial morphology was changed and mitochondrial membrane potential (MMP) was reduced, suggesting
  • found that prenatal exposure to TiO2 NPs could alter the expression of neurotransmitter genes as well as genes associated with apoptosis, OS, and psychiatric disorders. TiO2 NPs decreased cell viability in PC12 cells in a dose- and time-dependent manner by increasing the level of ROS and proportion of
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Published 29 Apr 2016

An ISA-TAB-Nano based data collection framework to support data-driven modelling of nanotoxicology

  • Richard L. Marchese Robinson,
  • Mark T. D. Cronin,
  • Andrea-Nicole Richarz and
  • Robert Rallo

Beilstein J. Nanotechnol. 2015, 6, 1978–1999, doi:10.3762/bjnano.6.202

Graphical Abstract
  • business rule no. 10 (see section 4 and Supporting Information File 4 for an in-depth explanation). The lethal cytotoxicity Assay file template (“a_InvID_cytotoxicity.cell-viability_Method.xls”) was designed to record data corresponding to a reduction in cellviability” (typically interpreted as an
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Published 05 Oct 2015
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