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Search for "cell viability" in Full Text gives 180 result(s) in Beilstein Journal of Nanotechnology.
Oxygen-plasma-modified biomimetic nanofibrous scaffolds for enhanced compatibility of cardiovascular implants
Beilstein J. Nanotechnol. 2015, 6, 254–262, doi:10.3762/bjnano.6.24
- performed to study the resistance of the plasma-treated scaffolds to plastic deformation. Lastly, the cell studies indicated that all scaffolds were cytocompatible, with the plasma-treated ones expressing a more pronounced cell viability and adhesion. All the above findings demonstrate the great potential
- scaffolds. Cell viability assay MTT cell viability assay was performed to the unmodified along with the plasma-treated (P = 20 W) scaffold, in order to evaluate the effect of the surface modification on the cellular performance of the fabricated samples in terms of cell viability. The cells used in the
- the fabricated systems were found to be cytocompatible after a period of seven days and exhibited cell viability levels similar to those of the control group. An enhancement in the cell viability (ca. 10%) can be observed in the case of the treated PCL scaffolds after seven days. The improved cellular
Mechanical properties of MDCK II cells exposed to gold nanorods
Beilstein J. Nanotechnol. 2015, 6, 223–231, doi:10.3762/bjnano.6.21
- /bjnano.6.21 Abstract Background: The impact of gold nanoparticles on cell viability has been extensively studied in the past. Size, shape and surface functionalization including opsonization of gold particles ranging from a few nanometers to hundreds of nanometers are among the most crucial parameters
- (DNA, proteins, antibodies) with functional groups using self-assembly techniques relying on gold–thiol interaction. Since these NPs are engineered to interact with living cells it is essential to prove if there is no adverse impact on cell viability [5][10]. Prerequisite for successful medical
- applications is the design of biocompatible NPs that do not impair with cell viability, proliferation, and adhesion. Therefore assessing the cytotoxicity of nanoparticles is pivotal for nanoparticle research in general [11]. In vitro nanocytotoxicity studies are therefore necessary to minimize possible risks
Caveolin-1 and CDC42 mediated endocytosis of silica-coated iron oxide nanoparticles in HeLa cells
Beilstein J. Nanotechnol. 2015, 6, 167–176, doi:10.3762/bjnano.6.16
- numbers were observed, which confirmed no severe impact of the treatment on cell viability within the observation period. Quantitative iron analysis For quantitative determination of iron, which was taken up by cells and not attached to the plastic surface or to the outer cell membrane, cells were washed
Synthesis of boron nitride nanotubes and their applications
Beilstein J. Nanotechnol. 2015, 6, 84–102, doi:10.3762/bjnano.6.9
- , 100 µg/mL of GC–BNNTs significantly decreased the cell viability. It was also found that the ROS production was not significant [74]. The hemolytic and cytotoxic effects of pure BNNTs on the malignant U87 (wild type p53), T98 (mutant p53) glioblastoma, MCF-7 adenocarcinoma mammary gland cells and
- related to the cell type and the ability to perform endocytosis [77]. The morphology and viability of the GC–BNNTs-exposed HUVECs cells were also investigated [71]. The cells were incubated at increasing concentrations of GC–BNNTs for 48 and 72 h. The cell morphology and cell viability by amido black
- for such an improvement was attributed to the formation of BNNT bridges among the polymeric structures. Figure 7 shows such structures as marked in red. When the osteoblast cell viability was evaluated with respect to the BNNT–PLC composite, and compared only to PLC, an increased cell viability was
Mammalian cell growth on gold nanoparticle-decorated substrates is influenced by the nanoparticle coating
Beilstein J. Nanotechnol. 2014, 5, 2479–2488, doi:10.3762/bjnano.5.257
- , cell adherence alone was not an adequate marker of cell viability, because due to the complexation properties of CTAB, the outer cell structure might have been fixed by the surfactant, which preserved the shape, while proliferation was prohibited. In order to have a reliable measure on viability, a
Proinflammatory and cytotoxic response to nanoparticles in precision-cut lung slices
Beilstein J. Nanotechnol. 2014, 5, 2440–2449, doi:10.3762/bjnano.5.253
- polyvinylpyrrolidone (PVP)-coated Ag-NPs under submerged culture conditions in vitro. ZnO-NPs (NM110) served as ‘soluble’ and quartz particles (Min-U-Sil) as ‘non-soluble’ control particles. After 4 and 24 h, the cell viability and the release of proinflammatory cytokines was measured. In addition, multiphoton
- microscopy was employed to assess the localization of Ag-NPs in PCLS after 24 h of incubation. Exposure of PCLS to ZnO-NPs for 4 and 24 h resulted in a strong decrease in cell viability, while quartz particles had no cytotoxic effect. Moreover, only a slight cytotoxic response was detected by LDH release
- staining, and WST-1 assay. As LDH is present in the cytoplasm of cells, detection of LDH in the culture medium of PCLS indicates a loss of cell membrane integrity. Therefore, LDH release is a direct measure of a cytotoxic response or an indirect measure of cell viability. As displayed in Figure 1, the LDH
Functionalized polystyrene nanoparticles as a platform for studying bio–nano interactions
Beilstein J. Nanotechnol. 2014, 5, 2403–2412, doi:10.3762/bjnano.5.250
- treatment with positively charged PS-NH2 particles (Figure 3). We have previously shown that neither PS-COOH nor PS-NH2 polystyrene nanoparticles affect cell viability when added to cells only for one day [43]. In addition, PS-COOH particles exhibit no toxic effect on macrophages, THP-1 or differentiated
- stained with acridine orange (AO) and analyzed by flow cytometry. M1 gating was used to assess the number of AOlow cells with leaky lysosomes. (B) Analysis of cell viability. Cells were treated as in (A) and analyzed by XTT assay. Results are mean ± SEM, n = 3, *p < 0.05, **p < 0.01. Acknowledgements
Nanoparticle interactions with live cells: Quantitative fluorescence microscopy of nanoparticle size effects
Beilstein J. Nanotechnol. 2014, 5, 2388–2397, doi:10.3762/bjnano.5.248
- cells [20]. Typically, live cells are immersed in a medium supplemented with blood serum to ensure cell viability. NPs in the medium form a biomolecular adsorption layer that depends on the composition of the medium. Therefore, the outcome of these experiments strongly depends on the choice of medium
Interaction of dermatologically relevant nanoparticles with skin cells and skin
Beilstein J. Nanotechnol. 2014, 5, 2363–2373, doi:10.3762/bjnano.5.245
- cellular uptake, including optical microscopy, electron microscopy, X-ray microscopy on cells and tissue sections, flow cytometry of isolated skin cells as well as Raman microscopy on whole tissue blocks. In order to assess the biological relevance of such findings, cell viability and free radical
- influence of AgNP exposure on cell viability, we investigated the influence of AgNP on cell metabolism in HaCaT cells by the XTT assay based on 2,3-bis(2-methoxy-4-nitro-5-sulfophenyl)-5-((phenylamino)carbonyl)-2H-tetrazolium hydroxid (Figure 4a). Interestingly, the XTT signal increased in cells incubated
- with low concentrations of Ag (<20 µg/mL) but it decreased for cells incubated with 40 µg/mL. When the serum concentration in the cell culture media was reduced below the required amount of 9%, all concentrations of AgNP induced a reduction in cell viability. This finding is relevant with regard to
Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells
Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239
- and to the environment have been of interest for some time [3][4][5]. Also, numerous in vitro, less in vivo studies and some case reports described adverse effects caused by NPs on cell viability, protein functions and DNA stability as well as other cell and tissue impairments [6][7][8][9][10]. In the
- , the transwell filters were embedded in Mowiol-Dabco (10% (w/w) Mowiol 4-88, 25% (w/w) glycerol, 2.5% (w/w) 1,4-diazabicyclo[2.2.2]octane, 0.1 M Tris-HCl, pH 8.5) on object slides. In all binding studies, cell viability was assessed by microscopical inspection of cell morphology after every incubation
Effect of silver nanoparticles on human mesenchymal stem cell differentiation
Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214
- in hMSCs at an early time-point (day 10) after osteogenic induction in the presence of Ag-NP [49]. The authors found no differences with respect to control cells without Ag-NP exposure, although a decrease in cell viability was also observed at Ag-NP concentrations ≥10 μg·mL−1. Pauksch et al
- were compared with cells cultured without differentiation medium in the presence of RPMI/10% FCS and Ag-NP/Ag+ ions and with cells incubated without Ag-NPs/ Ag+ ions in the presence of the differentiation medium alone (100% differentiation). Measurement of cell viability The viability of the incubated
PVP-coated, negatively charged silver nanoparticles: A multi-center study of their physicochemical characteristics, cell culture and in vivo experiments
Beilstein J. Nanotechnol. 2014, 5, 1944–1965, doi:10.3762/bjnano.5.205
Imaging the intracellular degradation of biodegradable polymer nanoparticles
Beilstein J. Nanotechnol. 2014, 5, 1905–1917, doi:10.3762/bjnano.5.201
- specified concentrations. Flow cytometry Flow cytometry was used for quantification of intracellular nanoparticles and for the analysis of cell viability. Similar to the procedures previously described [26], adherent cells were detached by trypsin (Gibco, Germany) and seeded in α-MEM at a density of 100 000
- , flow cytometry measurements revealed that the same magnetite-labeled PLLA particles did not affect the viability of the MSCs. This has been shown in our previous works [27]. PLLA particles with an even higher iron concentration did not affect the cell viability over a period of 6 days. Confocal laser
Carbon-based smart nanomaterials in biomedicine and neuroengineering
Beilstein J. Nanotechnol. 2014, 5, 1849–1863, doi:10.3762/bjnano.5.196
- slightly affected cell viability, even for a concentration of about 400 μg/mL. Schrand and co-workers [70][71][72] extensively studied this issue through standard in vitro cell viability assays (i.e., MTT) and also monitored adenosine triphosphate (ATP) production and ROS generation. They found that
Biocompatibility of cerium dioxide and silicon dioxide nanoparticles with endothelial cells
Beilstein J. Nanotechnol. 2014, 5, 1795–1807, doi:10.3762/bjnano.5.190
- ng/mL, 10 µg/mL, 100 µg/mL) for defined incubation times (3, 24, 48 and 72 h). Afterwards, the cells were washed with Hank’s BSS (PAA Laboratories GmbH, Austria) and the CellTiter-Glo® Luminescent Cell Viability Assay (Promega GmbH, Germany) was carried out according to the manufacturer’s
The surface properties of nanoparticles determine the agglomeration state and the size of the particles under physiological conditions
Beilstein J. Nanotechnol. 2014, 5, 1774–1786, doi:10.3762/bjnano.5.188
- were shown to exhibit a dose-dependent cytotoxicity, whereas PEGylated poly(organosiloxane) NPs do not reduce the cell viability [16]. As emphasized in the introductory part of the results section, verification of the nanomaterial properties in biologically relevant media is crucial to correlate the
Influence of surface-modified maghemite nanoparticles on in vitro survival of human stem cells
Beilstein J. Nanotechnol. 2014, 5, 1732–1737, doi:10.3762/bjnano.5.183
- [30]. The concentration-dependent effect of D-mannose- and PDMAAm-coated γ-Fe2O3 nanoparticles on the cell viability was determined after incubation for 72 h and compared with the cells in the absence of nanoparticles (control experiment). All investigated γ-Fe2O3 nanoparticles exhibited a cytotoxic
Precise quantification of silica and ceria nanoparticle uptake revealed by 3D fluorescence microscopy
Beilstein J. Nanotechnol. 2014, 5, 1616–1624, doi:10.3762/bjnano.5.173
- properties have been described as beneficial applications in nanomedicine [17][18][19]. On the other hand, oxidative stress and impaired cell viability were shown to be a function of the particle dose and the exposure time [1][20]. However, most of the studies concerning the interaction of silica and ceria
In vitro interaction of colloidal nanoparticles with mammalian cells: What have we learned thus far?
Beilstein J. Nanotechnol. 2014, 5, 1477–1490, doi:10.3762/bjnano.5.161
- geometry, in which the culture medium is on top of the cells, a reduced colloidal stability leads to the precipitation of NP agglomerates onto the cells and, thus, to enhanced uptake, which can influence the cell viability negatively [89]. Such different exposure scenarios are highly relevant for the
Protein-coated pH-responsive gold nanoparticles: Microwave-assisted synthesis and surface charge-dependent anticancer activity
Beilstein J. Nanotechnol. 2014, 5, 1452–1462, doi:10.3762/bjnano.5.158
- also observed in the UV–vis studies for the blank proteins (Figure S3, Supporting Information File 1). Cell viability after exposure to AuNPs To investigate the cell viability after the exposure to AuNPs, MTT assays were conducted with mouse embryonic fibroblasts (NIH-3T3) by treating them with AuNPs
- samples prepared by using different proteins (Figure 4A). Exposure to AuNPs resulted in different cell viability values based on the protein used for capping. The OVA-coated AuNPs had the highest IC50 value, and HIS-AuNPs had the lowest. From the zeta potential titration studies (Figure 3), HIS-AuNPs had
- of the different AuNPs revealed that the cell viabilities after exposure to the AuNPs decreased with increasing IEP values. Therefore, the HIS-AuNPs, which had an IEP value of 10.58, resulted in the lowest cell viability, leading to high toxicity. The OVA-AuNPs, which had an IEP value of 5.08
The protein corona protects against size- and dose-dependent toxicity of amorphous silica nanoparticles
Beilstein J. Nanotechnol. 2014, 5, 1380–1392, doi:10.3762/bjnano.5.151
- established a dual-color fluorescence cell vitality assay. By employing fluorescent probes that recognize cell viability by measuring intracellular esterase activity (calcein-AM; green) as well as plasma membrane integrity (ethidium homodimer-1/EthD-1; red), the assay allows for the simultaneous quantitation
- vitality of Caco-2, automated high-throughput microscopy revealed that cell death was almost completely prevented upon treatment in the presence of serum or human plasma proteins (Figure 4, right panel). Similar results were obtained by assessing the cell viability using independent methods, such as the
- living cells, image analysis and presentation were performed as described in detail in [53]. Measurement of cell viability Cell viability was determined by using the electric sensing zone method (CASY® TT Cell Counter; Schärfe SystemGmbH, Reutlingen, Germany) or by the mitochondria-dependent reduction of
Model systems for studying cell adhesion and biomimetic actin networks
Beilstein J. Nanotechnol. 2014, 5, 1193–1202, doi:10.3762/bjnano.5.131
- shape and for maintaining cell viability, migration, and tissue integrity [3]. Cell–cell and cell–substrate adhesion are mediated by different proteins, the cell adhesion molecules (CAMs). A very important group of CAMs is the integrin family, which functions both as cell–substrate and cell–cell
Nanodiamond-DGEA peptide conjugates for enhanced delivery of doxorubicin to prostate cancer
Beilstein J. Nanotechnol. 2014, 5, 937–945, doi:10.3762/bjnano.5.107
- effects of the drug delivery system were due to the combination of enhanced targeting and drug delivery instead of potential toxicity of ND or DGEA peptide, PC3 cells were also exposed to ND, ND-DGEA, and free DGEA. The cell viability was quantified with a 3-(4,5-dimethylthiazol-2-yl)-5-(3
- -carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS, Promega, Madison, WI) assay. Briefly, MTS assay reagent was added to the cells, and the plates were incubated for 2 h. The absorbance at 490 nm was read with a microplate reader. Cell viability was represented as percentages in reference to the control
- exposed to these treatments for 32 h, and MTS cell viability assay was performed. As shown in Figure 6a, there were no significant differences in cell viability for any of the treatments; the cell viabilities for NDs, DGEA, and ND-DGEA conjugates were all comparable to the control. With the demonstration
Optimizing the synthesis of CdS/ZnS core/shell semiconductor nanocrystals for bioimaging applications
Beilstein J. Nanotechnol. 2014, 5, 919–926, doi:10.3762/bjnano.5.105
- studies, a cell viability (MTS) assay was carried out for F127-CdS/ZnS QDs. As shown in Figure 10, we tested the cell viability of Panc-1 cells, treating them with various concentrations of ternary nanocrystal formulations for 48 h. The cell viability remained at 84% even at a concentration as high as 500
- for in vitro and in vivo studies. In addition, from our study of cell viability, the prepared ternary nanocrystals were shown to have a low cytotoxicity. Given their biocompatibility and the high efficiency in relation to targeted delivery, these nanoparticles have great potential to serve as a new
- (FBS, Hyclone) and cultured at 37 °C in a humidified atmosphere with 5% CO2. For cell imaging, cells were treated with F127-CdS/ZnS micelles formulations for 2 h. The cells were then washed with PBS for three times and imaged using a Leica confocal microscopy system (TCS SP2). For the cell viability
Antimicrobial nanospheres thin coatings prepared by advanced pulsed laser technique
Beilstein J. Nanotechnol. 2014, 5, 872–880, doi:10.3762/bjnano.5.99
- secondary electron beams with energies of 30 keV on samples covered with a thin gold layer. Cell viability Human endothelial cells (EAhy926 cell line, ATCC, USA) were grown in Dulbecco's Modified Eagle Medium (DMEM) culture medium containing 10% Fetal Bovine Serum (FBS), and 1% penicillin and neomycin
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