Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• mentioned spice-derived C-dots was evaluated in vitro in human glioblastoma cells (LN-229 cancer cell line) and in human kidney cells (HK-2 non-cancerous cell line). Bioimaging and viability studies were performed with different C-dot concentrations from 0.1 to 2 mg·mL−1, exhibiting a higher uptake of C

• -dots in the cancer cultures compared to the non-cancerous cells. Results showed that the spice-derived C-dots inhibited cell viability dose-dependently after a 24 h incubation period, displaying a higher toxicity in LN-229, than in HK-2 cells. As a control, C-dots synthesized from citric acid did not

• instance, piperine, a major chemical compound present in black pepper, has shown anti-inflammatory, anti-angiogenic, and anti-arthritic effects [15]. Moreover, it has been reported that black pepper is capable to reduce breast cancer cell proliferation [16][17][18]. Turmeric is an abundant medicinal herb

Nanoparticle delivery to metastatic breast cancer cells by nanoengineered mesenchymal stem cells

• Liga Saulite,
• Karlis Pleiko,
• Ineta Popena,
• Dominyka Dapkute,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2018, 9, 321–332, doi:10.3762/bjnano.9.32

• Liga Saulite Karlis Pleiko Ineta Popena Dominyka Dapkute Ricardas Rotomskis Una Riekstina Faculty of Medicine, University of Latvia, Raina Blvd. 19, LV-1586 Riga, Latvia Biomedical Physics Laboratory, National Cancer Institute, P. Baublio Street 3b, LT-08406 Vilnius, Lithuania Life Science Center

• metastatic breast cancer cell line MDA-MD-231 and primary breast cancer cell line MCF7 to explore the transfer of quantum dots (QDs) to cancer cells. First, the optimal conditions for high-content QD loading in MSCs were established. Then, QD uptake in breast cancer cells was assessed after 24 h in a 3D co

• -culture with nanoengineered MSCs. We found that incubation of MSCs with QDs in a serum-free medium provided the best accumulation results. It was found that 24 h post-labelling QDs were eliminated from MSCs. Our results demonstrate that breast cancer cells efficiently uptake QDs that are released from

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• is, in most cases, still the ultimate cause of death once it is diagnosed. Similar to the treatment of other types of cancer, PC treatment suffers on an accurate and efficient application of active substances to affected regions. In order to treat PC with adequately high doses of chemotherapeutic

• to PIPAC-MIP, HINAT provides the opportunity to perform further studies concerning the treatment of peritoneal carcinomatosis or other aerosol-accessible kinds of cancer (e.g., lung cancer) on animals even smaller than pigs due to the minimal required access space for feeding nanometre-sized aerosols

Fabrication of gold-coated PDMS surfaces with arrayed triangular micro/nanopyramids for use as SERS substrates

• Jingran Zhang,
• Yongda Yan,
• Peng Miao and
• Jianxiong Cai

Beilstein J. Nanotechnol. 2017, 8, 2271–2282, doi:10.3762/bjnano.8.227

• detection of chemical molecules and biological species at low concentrations. SERS has recently attracted wide-ranging attention from researchers in many fields, including biosensing and bioanalysis [1][2][3], cancer cell work [4], virus identification [5][6], food safety [7][8] and gas vapor research [9

Evaluating the toxicity of TiO₂-based nanoparticles to Chinese hamster ovary cells and Escherichia coli: a complementary experimental and computational approach

• Alicja Mikolajczyk,
• Natalia Sizochenko,
• Ewa Mulkiewicz,
• Anna Malankowska,
• Michal Nischk,
• Przemyslaw Jurczak,
• Seishiro Hirano,
• Grzegorz Nowaczyk,
• Adriana Zaleska-Medynska,
• Jerzy Leszczynski,
• Agnieszka Gajewicz and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2017, 8, 2171–2180, doi:10.3762/bjnano.8.216

• a water bath for 30 min at 37 °C. Cytotoxicity was determined using the Chinese hamster ovary cell line (CHO-K1) (ATCC® CCL-61™). The sensitivity of three different cell lines: CHO-K1 and two human lung (cancer and normal) cell lines (A549, BEAS-2B) to the tested nanomaterials was studied in a

Synthesis and characterization of noble metal–titania core–shell nanostructures with tunable shell thickness

• Bartosz Bartosewicz,
• Marta Michalska-Domańska,
• Malwina Liszewska,
• Dariusz Zasada and
• Bartłomiej J. Jankiewicz

Beilstein J. Nanotechnol. 2017, 8, 2083–2093, doi:10.3762/bjnano.8.208

• tunable optical properties depending on the ratio of core radius and shell thickness are an excellent example of such structures [3][4]. The CSNs, with either a silica core or shell, have found many applications due to their useful properties, including surface enhanced spectroscopy or cancer therapy [4

Carbon nano-onions as fluorescent on/off modulated nanoprobes for diagnostics

• Stefania Lettieri,
• Marta d’Amora,
• Adalberto Camisasca,
• Alberto Diaspro and
• Silvia Giordani

Beilstein J. Nanotechnol. 2017, 8, 1878–1888, doi:10.3762/bjnano.8.188

• changes in the cells and tissues. Some of these events include cell proliferation and apoptosis [1], ion transport [2] and other cellular process and diseases such as cancer [3][4][5], Parkinson's, and Alzheimer's disease [6]. Despite the large number of nanotechnology platforms available to date for

• recent reports have shown that CNOs exhibit weak inflammatory potential and low cytotoxicity [16], and they are readily internalized by cancer cells and localize in the lysosomes [18][19]. Moreover, our in vivo studies performed on zebrafish (Danio Rerio) during the development stage demonstrated their

Optical techniques for cervical neoplasia detection

• Tatiana Novikova

Beilstein J. Nanotechnol. 2017, 8, 1844–1862, doi:10.3762/bjnano.8.186

• emerging technologies. Using colposcopy, a visual inspection of the uterine cervix with a colposcope (a binocular microscope with 3- to 15-fold magnification), has proven to be an efficient approach for the detection of invasive cancer. Nevertheless, the development of a reliable and cost-effective

• endomicroscopy; Mueller polarimetry; nanotheranostics; optical coherence tomography; optical spectroscopy; Raman spectroscopy; Review Introduction Cervical cancer remains one of the major health issues, causing 266000 deaths of women worldwide in 2012 [1]. While the highest incidence rate of cervical cancers

• (approximately 70%) is observed in developed countries, the cervical cancer mortality rate is highest in low-income countries, where the regular screening by Papanicolaou (Pap) test, colposcopy, biopsy and curative treatment are not routinely available because of lack of health infrastructure, trained

Synthesis and functionalization of NaGdF₄:Yb,Er@NaGdF₄ core–shell nanoparticles for possible application as multimodal contrast agents

• Dovile Baziulyte-Paulaviciene,
• Vitalijus Karabanovas,
• Marius Stasys,
• Greta Jarockyte,
• Vilius Poderys,
• Simas Sakirzanovas and
• Ricardas Rotomskis

Beilstein J. Nanotechnol. 2017, 8, 1815–1824, doi:10.3762/bjnano.8.183

• Dovile Baziulyte-Paulaviciene Vitalijus Karabanovas Marius Stasys Greta Jarockyte Vilius Poderys Simas Sakirzanovas Ricardas Rotomskis Faculty of Chemistry and Geosciences, Vilnius University, Naugarduko str. 24, Vilnius LT-03225, Lithuania Biomedical Physics Laboratory, National Cancer Institute

• , Tween 80-coated core–shell nanoparticles presented enhanced optical and MR signal intensity, good colloidal stability, low cytotoxicity and nonspecific internalization into two different breast cancer cell lines, which indicates that these nanoparticles could be applied as an efficient, dual-modal

• contrast probe for in vivo bioimaging. Keywords: cancer theranostics; core–shell structure; luminescence; multimodal; nanoparticles; upconverting nanoparticles; upconversion; Introduction Lanthanide-doped multimodal upconverting nanoparticles (UCNPs), which can convert near-infrared (NIR) radiation into

Methionine-mediated synthesis of magnetic nanoparticles and functionalization with gold quantum dots for theranostic applications

• Arūnas Jagminas,
• Agnė Mikalauskaitė,
• Vitalijus Karabanovas and
• Jūrate Vaičiūnienė

Beilstein J. Nanotechnol. 2017, 8, 1734–1741, doi:10.3762/bjnano.8.174

• Arunas Jagminas Agne Mikalauskaite Vitalijus Karabanovas Jurate Vaiciuniene State Research Institute Center for Physical Sciences and Technology, Sauletekio Ave. 3, LT- 10222, Vilnius, Lithuania National Cancer Institute, Baublio 3b, LT- 08406, Vilnius, Lithuania 10.3762/bjnano.8.174 Abstract

• conjugated with targeting and chemotherapy agents, such as cancer stem cell-related antibodies and the anticancer drug doxorubicin, for early detection and improved treatment. In order to verify our findings, high-resolution transmission electron microscopy (HRTEM), atomic force microscopy (AFM), FTIR

• nanomedicine, biocompatible iron oxide-based NPs have attracted particular interest due to their size-dependent magnetic, optical and chemical properties that allow for the design of NPs for multimodal imaging and photothermal therapy of cancer cells [1]. Dual-imaging probes, capable to perform simultaneously

Near-infrared-responsive, superparamagnetic Au@Co nanochains

• Varadee Vittur,
• Arati G. Kolhatkar,
• Shreya Shah,
• Irene Rusakova,
• Dmitri Litvinov and
• T. Randall Lee

Beilstein J. Nanotechnol. 2017, 8, 1680–1687, doi:10.3762/bjnano.8.168

• -thermal cancer therapy [10][11][12][13]. Results and Discussion Morphology and composition of the Au@Co nanochains The synthesis of the Au@Co nanochains proceeded using wet-chemical methods via a galvanic replacement reaction [27]. Cobalt nanoparticles were used as templates for the nanochain synthesis

• -thermal cancer therapy. In addition, these nanochains are superparamagnetic at room temperature, which offers the capacity for manipulation under an external magnetic field. Because of their dual magneto-optical properties, our Au@Co nanochains can be used for both delivery and treatment, making them a

Process-specific mechanisms of vertically oriented graphene growth in plasmas

• Subrata Ghosh,
• Shyamal R. Polaki,
• Niranjan Kumar,
• Sankarakumar Amirthapandian,
• Mohamed Kamruddin and
• Kostya (Ken) Ostrikov

Beilstein J. Nanotechnol. 2017, 8, 1658–1670, doi:10.3762/bjnano.8.166

• properties of VGNs. The culturing rate of cancer cervical cells was found to strongly depend on the density of VGNs by Watanabe and co-workers [13]. Recently, Bo et al. [14] reported the tunability of wetting properties from hydrophobic to hydrophilic by reducing the intersheet spacing of VGNs for enhanced

Uptake and intracellular accumulation of diamond nanoparticles – a metabolic and cytotoxic study

• Antonín Brož,
• Lucie Bačáková,
• Pavla Štenclová,
• Alexander Kromka and
• Štěpán Potocký

Beilstein J. Nanotechnol. 2017, 8, 1649–1657, doi:10.3762/bjnano.8.165

• impaired the radio-resistance of cancer cells and potentiated radiation-caused DNA damage and the generation of cytotoxic reactive oxygen species [47]. Thus, the positive charge of our as-received DNDs could, at least partly, explain their more pronounced cytotoxic effect than that observed in negatively

A nanocomplex of C₆₀ fullerene with cisplatin: design, characterization and toxicity

• Svitlana Prylutska,
• Svitlana Politenkova,
• Kateryna Afanasieva,
• Volodymyr Korolovych,
• Kateryna Bogutska,
• Andriy Sivolob,
• Larysa Skivka,
• Maxim Evstigneev,
• Viktor Kostjukov,
• Yuriy Prylutskyy and
• Uwe Ritter

Beilstein J. Nanotechnol. 2017, 8, 1494–1501, doi:10.3762/bjnano.8.149

• , cisplatin (cis-[Pt(II)(NH3)2Cl2], Cis), is currently one of the most effective therapeutic agents used against cancer deceases, in particular, ovarian cancer, bladder cancer, esophagus cancer, lung cancer, and cancer of head and neck [1]. As an antitumor metal-containing agent Cis exerts an alkylating

• concentrations), exerting strong antioxidant properties due to their high activity as free radical acceptors [14][15]. Water-soluble pristine С60 fullerenes penetrate through plasma membranes and are located in the central part of tumor cells [16]. Thereby, C60 fullerenes can be used for treatment of cancer [17

• complexation could induce biological synergy for other drugs administered together with C60 fullerene as well [19][23]. Taking into account the importance of Cis in chemotherapy of cancer, this drug could be a candidate molecule for study. A recent extended physico-chemical study has confirmed the formation of

Development of polycationic amphiphilic cyclodextrin nanoparticles for anticancer drug delivery

• Gamze Varan,
• Juan M. Benito,
• Carmen Ortiz Mellet and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1457–1468, doi:10.3762/bjnano.8.145

• -cytotoxic against L929 mouse fibroblast cell line. In addition, paclitaxel-loaded nanoparticles have a significant anticancer effect against MCF-7 human breast cancer cell line as compared with a paclitaxel solution in DMSO. Conclusion: According to the results of this study, both amphiphilic cyclodextrin

• of chemotherapy with PCX. A promising step was taken with the FDA approval of albumin nanoparticle bound PCX (Abraxane®) in 2005 for breast cancer treatment with a significantly lower dose [8]. This was considered a breakthrough in PCX formulation development as it avoided the use of solubilizers

• their drug encapsulation, release profile and anticancer activity on MCF-7 human breast cancer cell line in particular. Safety and apoptotic efficacy of blank and PCX-loaded cationic or anionic amphiphilic CD nanoparticles were evaluated with cell culture studies against a series of healthy and cancer

Cationic PEGylated polycaprolactone nanoparticles carrying post-operation docetaxel for glioma treatment

• Cem Varan and
• Erem Bilensoy

Beilstein J. Nanotechnol. 2017, 8, 1446–1456, doi:10.3762/bjnano.8.144

• , facilitating chemotherapy administration to prevent recurrence of the tumor at the time of tumor tissue removal by surgical operation. Among the anticancer drugs that are used in clinics, the taxane family of drugs such as paclitaxel and docetaxel are known to be highly effective against a variety of cancer

• often cause serious side effects. Thus, the necessity of a safe and effective formulation and drug delivery approach emerges for these potent anticancer drugs from the taxane family [10][11][12][13]. Successful treatment of brain cancer is dependent on the efficient and safe delivery of chemotherapeutic

• that active targeted PEG/PCL nanoparticles enhanced tumor penetration [22]. Besides that, Ungaro et al. obtained docetaxel-loaded core–shell PEO/PCL nanoassemblies for passive targeting of the anticancer drug to cancer cells. Their results showed that docetaxel-loaded PEO/PCL nanoparticles were more

Carbon nanomaterials sensitize prostate cancer cells to docetaxel and mitomycin C via induction of apoptosis and inhibition of proliferation

• Kati Erdmann,
• Jessica Ringel,
• Silke Hampel,
• Manfred P. Wirth and
• Susanne Fuessel

Beilstein J. Nanotechnol. 2017, 8, 1307–1317, doi:10.3762/bjnano.8.132

• , Dresden 01171, Germany 10.3762/bjnano.8.132 Abstract We have previously shown that carbon nanofibers (CNFs) and carbon nanotubes (CNTs) can sensitize prostate cancer (PCa) cells to platinum-based chemotherapeutics. In order to further verify this concept and to avoid a bias, the present study

• in combination with DTX and MMC evoked additive to partly synergistic anti-tumor effects. CNFs and CNTs possess the ability to sensitize cancer cells to a wide range of structurally diverse chemotherapeutics and thus represent an interesting option for the development of multimodal cancer therapies

• . Co-administration of chemotherapeutics with carbon nanomaterials could result in a reduction of the chemotherapeutic dosage and thus limit systemic side effects. Keywords: carbon nanomaterials; chemosensitization; docetaxel; mitomycin C; prostate cancer cells; Introduction According to the global

Bright fluorescent silica-nanoparticle probes for high-resolution STED and confocal microscopy

• Isabella Tavernaro,
• Christian Cavelius,
• Henrike Peuschel and
• Annette Kraegeloh

Beilstein J. Nanotechnol. 2017, 8, 1283–1296, doi:10.3762/bjnano.8.130

• drug carriers [3][4][5], as transfection agents [6][7], for cancer treatment by local hyperthermia [8][9], for labelling [10][11] and for bioimaging [12][13][14]. The detection of cell-associated and internalised nanoparticles and the analysis of their interactions with extracellular or subcellular

Nano-engineered skin mesenchymal stem cells: potential vehicles for tumour-targeted quantum-dot delivery

• Liga Saulite,
• Dominyka Dapkute,
• Karlis Pleiko,
• Ineta Popena,
• Simona Steponkiene,
• Ricardas Rotomskis and
• Una Riekstina

Beilstein J. Nanotechnol. 2017, 8, 1218–1230, doi:10.3762/bjnano.8.123

• Liga Saulite Dominyka Dapkute Karlis Pleiko Ineta Popena Simona Steponkiene Ricardas Rotomskis Una Riekstina Faculty of Medicine, University of Latvia, Raina blvd. 19, LV-1586, Riga, Latvia Biomedical Physics Laboratory, National Cancer Institute, P. Baublio Street 3b, LT-08406 Vilnius, Lithuania

• suggest that QD-labelled MSCs could be used for targeted drug delivery studies. Keywords: endocytosis; mesenchymal stem cells; quantum dots; stem cell differentiation; Introduction Despite remarkable advances in targeted therapies of various human malignancies, cancer is one of the leading causes of

• QDs indicate their great potential in cancer diagnostics. The photoluminescence spectrum of carboxyl QD655 makes them ideal candidates for cancer theranostics as it overlaps with the optical transparency window of biological tissue [11]. Additionally, large and easily altered surfaces facilitate

Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification

• Mohamed Hassoun,
• Iwan W.Schie,
• Tatiana Tolstik,
• Sarmiza E. Stanca,
• Christoph Krafft and
• Juergen Popp

Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120

• to allow interaction between nanoparticles and bacterial biomolecules [24]. This gave very reproducible SERS spectra. The current study transfers this SERS approach to distinguish four human cancer cell lines. These cell lines are two liver cancer cell lines (HepG2 isolated from liver tissue of a

• male patient with well differentiated hepatocellular carcinoma and SK-Hep1 received from ascetic fluid of a patient with adenocarcinoma of the liver), one breast cancer cell line (MCF-7 obtained from a female patient) and one human pancreatic ductal adenocarcinoma cell line (Capan-1). A protocol was

• , specificities and accuracies of the SVM model were calculated by cross-validation schemes. This proof-of-principle demonstrates that non-functionalized, easy-to-prepare silver nanoparticles give reproducible SERS spectra that can be used for the identification of human cancer cells. Results and Discussion The

Needs and challenges for assessing the environmental impacts of engineered nanomaterials (ENMs)

• Michelle Romero-Franco,
• Hilary A. Godwin,
• Muhammad Bilal and
• Yoram Cohen

Beilstein J. Nanotechnol. 2017, 8, 989–1014, doi:10.3762/bjnano.8.101

• information, and the metrics include the establishment of reference doses (e.g., to protect the susceptible population from harmful effects), hazard index and cancer potency factors. By contrast, the bulk of available toxicity data for ENMs are mostly based on in vitro assays and modeling approaches (e.g

3D Nanoprinting via laser-assisted electron beam induced deposition: growth kinetics, enhanced purity, and electrical resistivity

• Brett B. Lewis,
• Robert Winkler,
• Xiahan Sang,
• Pushpa R. Pudasaini,
• Michael G. Stanford,
• Harald Plank,
• Raymond R. Unocic,
• Jason D. Fowlkes and
• Philip D. Rack

Beilstein J. Nanotechnol. 2017, 8, 801–812, doi:10.3762/bjnano.8.83

• detection [4], the study of biological systems important in determining cancer treatment options [5], and reliable, low cost, high performance magnetic hard disk drives [6]. A variety of fabrication techniques have been used to construct multi-dimensional nanostructures [7][8][9][10] with differing degrees

Modeling adsorption of brominated, chlorinated and mixed bromo/chloro-dibenzo-p-dioxins on C₆₀ fullerene using Nano-QSPR

• Piotr Urbaszek,
• Agnieszka Gajewicz,
• Celina Sikorska,
• Maciej Haranczyk and
• Tomasz Puzyn

Beilstein J. Nanotechnol. 2017, 8, 752–761, doi:10.3762/bjnano.8.78

• applications, including sorbents, cancer therapeutics, drug delivery systems, computer sensors, etc. [16][17][18][19]. With the further development of nanotechnology, C60 will be produced and used in large amounts. Over time, fullerene structures will be found in the environment more often and in higher

Phospholipid arrays on porous polymer coatings generated by micro-contact spotting

• Sylwia Sekula-Neuner,
• Monica de Freitas,
• Lea-Marie Tröster,
• Tobias Jochum,
• Pavel A. Levkin,
• Michael Hirtz and
• Harald Fuchs

Beilstein J. Nanotechnol. 2017, 8, 715–722, doi:10.3762/bjnano.8.75

• of prostate cancer [19]. AR contributes to breast cancer progression and development [20] and its mutation is involved in the progression of neurodegenerative diseases, such as the X-linked spinal and bulbar muscular atrophy [21]. In order to better understand and characterize the behavior and

• the therapeutic treatment for breast and prostate cancer patients. Keeping in mind previous applications of HEMA polymer in creating superhydrophilic–superhydrophobic micropatterned surfaces for cell patterning [28] and cell-screening applications [29][30], the addition of lipid arraying to the

Dispersion of single-wall carbon nanotubes with supramolecular Congo red – properties of the complexes and mechanism of the interaction

• Anna Jagusiak,
• Barbara Piekarska,
• Tomasz Pańczyk,
• Małgorzata Jemioła-Rzemińska,
• Elżbieta Bielańska,
• Barbara Stopa,
• Grzegorz Zemanek,
• Janina Rybarska,
• Irena Roterman and
• Leszek Konieczny

Beilstein J. Nanotechnol. 2017, 8, 636–648, doi:10.3762/bjnano.8.68

• nanotubes (SWNTs) are currently intensely studied as promising drug delivery systems for cancer therapies due to such their properties as: the ability to penetrate cell membrane [16][17], high drug capacity [8][9], selective retention in the tumour [21], reduced toxic effects of the drug [5][20]. The major