Dual-functionalized architecture enables stable and tumor cell-specific SiO₂NPs in complex biological fluids

• Iris Renata Sousa Ribeiro,
• Raquel Frenedoso da Silva,
• Romênia Ramos Domingues,
• Adriana Franco Paes Leme and
• Mateus Borba Cardoso

Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100

• exhibit heightened efficacy and reduced toxicity for medical purposes. Keywords: colloidal stability; complex media; functionalized nanoparticles; hemolysis; targeting tumor; Introduction In recent years, there has been a growing search for developing high-efficiency nanomedicines for cancer treatment

Identification of structural features of surface modifiers in engineered nanostructured metal oxides regarding cell uptake through ML-based classification

• Indrasis Dasgupta,
• Totan Das,
• Biplab Das and
• Shovanlal Gayen

Beilstein J. Nanotechnol. 2024, 15, 909–924, doi:10.3762/bjnano.15.75

• iron oxide nanocore decorated with organic compounds and investigated their cellular uptake across various human cell types [18]. However, determining the cellular absorption of functionalized nanoparticles in different human cell types is a laborious, expensive, and time-consuming task. Computational

Curcumin-loaded nanostructured systems for treatment of leishmaniasis: a review

• Douglas Dourado,
• Thayse Silva Medeiros,
• Éverton do Nascimento Alencar,
• Edijane Matos Sales and
• Fábio Rocha Formiga

Beilstein J. Nanotechnol. 2024, 15, 37–50, doi:10.3762/bjnano.15.4

• observe a general decrease in IC50 when compared to free curc, which was mainly attributed to the cell uptake of these structures. Indeed, studies that functionalized nanostructures with mannose for an increase in macrophage phagocytosis evidenced that functionalized nanoparticles decreased IC50 when

Polymer nanoparticles from low-energy nanoemulsions for biomedical applications

• Santiago Grijalvo and
• Carlos Rodriguez-Abreu

Beilstein J. Nanotechnol. 2023, 14, 339–350, doi:10.3762/bjnano.14.29

• wt % PLGA in ethyl acetate/ethanol) (O) system were functionalized with a cell-penetrating peptide (Tat) using carbodiimide chemistry [57]. The hydrodynamic diameter ranged from 48 to 65 nm depending on the Tat fraction. The functionalized nanoparticles showed dose-dependent cytotoxicity on Hela and

• composed of carbosilane dendron functionalized nanoparticles prepared from nanoemulsions as non-viral carriers for antisense oligonucleotides”, pages 113-123, Copyright (2014), with permission from Elsevier. This content is not subject to CC BY 4.0. (a) Partial phase diagram of the system PBS/Polysorbate

Two-step single-reactor synthesis of oleic acid- or undecylenic acid-stabilized magnetic nanoparticles by thermal decomposition

• Mykhailo Nahorniak,
• Pamela Pasetto,
• Jean-Marc Greneche,
• Volodymyr Samaryk,
• Sandy Auguste,
• Anthony Rousseau,
• Nataliya Nosova and
• Serhii Varvarenko

Beilstein J. Nanotechnol. 2023, 14, 11–22, doi:10.3762/bjnano.14.2

• contrast to oleic acid, when using undecylenic acid in the synthesis, dispersions of magnetite particles of larger sizes (11–16 nm) are obtained, which have a large organic shell (up to 65% of the particle mass) protecting them from oxidation. Such stable dispersions of functionalized nanoparticles can be

Biocompatibility and cytotoxicity in vitro of surface-functionalized drug-loaded spinel ferrite nanoparticles

• Sadaf Mushtaq,
• Khuram Shahzad,
• Tariq Saeed,
• Anwar Ul-Hamid,
• Bilal Haider Abbasi,
• Nafees Ahmad,
• Waqas Khalid,
• Muhammad Atif,
• Zulqurnain Ali and
• Rashda Abbasi

Beilstein J. Nanotechnol. 2021, 12, 1339–1364, doi:10.3762/bjnano.12.99

• side effects of conventional therapeutic agents [4]. Functionalized nanoparticles have the potential to improve the therapeutic performance of drugs by regulating pharmacokinetics and pharmacodynamics [5]. Moreover, water compatibility of nanocarriers provides better chemical stability and

The impact of molecular tumor profiling on the design strategies for targeting myeloid leukemia and EGFR/CD44-positive solid tumors

• Nikola Geskovski,
• Nadica Matevska-Geshkovska,
• Simona Dimchevska Sazdovska,
• Marija Glavas Dodov,
• Kristina Mladenovska and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2021, 12, 375–401, doi:10.3762/bjnano.12.31

• localization of the functionalized nanoparticles relative to the non-functionalized nanoparticles. Additionally, the loaded PLGA–PEG–alendronate nanoparticles demonstrated satisfactory results in myeloma growth inhibition and overall survival of the murine models. Considering the toxicity of bortezomib

• addition, the treated B220+/Mac1− cells were injected in immunodeficient mice, and the median time for leukemia onset in the animals was observed for daunorubicin, daunorubicin-loaded non-functionalized nanoparticles, and anti-B220 antibody-functionalized nanoparticles loaded with daunorubicin. The

A review on the biological effects of nanomaterials on silkworm (Bombyx mori)

• Sandra Senyo Fometu,
• Guohua Wu,
• Lin Ma and
• Joan Shine Davids

Beilstein J. Nanotechnol. 2021, 12, 190–202, doi:10.3762/bjnano.12.15

• triethoxycaprylylsilane: 130 nm, size of non-functionalized nanoparticles: 100 nm). Conversely, mice exposed to 100 mg/mL of Ag NM (<20 nm) exhibited no adverse effects on the lungs [38]. Also, anatase TiO2 NM was reported to induce pulmonary inflammation in mice models [39]. Kim et al. reported that male Sprague-Dawley

Key for crossing the BBB with nanoparticles: the rational design

• Sonia M. Lombardo,
• Marc Schneider,
• Akif E. Türeli and
• Nazende Günday Türeli

Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72

• also accumulate in other organs [128][129]. Influence of size and zeta potential It has been shown that surface functionalization or surface coating are the most important determinants for BBB crossing. For functionalized nanoparticles, size seems to have little impact and nanoparticles in a large size

Internalization mechanisms of cell-penetrating peptides

• Ivana Ruseska and
• Andreas Zimmer

Beilstein J. Nanotechnol. 2020, 11, 101–123, doi:10.3762/bjnano.11.10

• different set of interactions that could induce the macropinocytosis of CPPs. In this case, the research group investigated the uptake of TAT-peptide functionalized nanoparticles and they proposed the CendR (C-end-rule) pathway as a possible mechanism that activates macropinocytosis. This route involves the

Advanced hybrid nanomaterials

• Andreas Taubert,
• Fabrice Leroux,
• Pierre Rabu and
• Verónica de Zea Bermudez

Beilstein J. Nanotechnol. 2019, 10, 2563–2567, doi:10.3762/bjnano.10.247

• and then embedded into a silicone polymer. These coatings deliver white-light emission when placed above a blue LED. Health Functionalized nanoparticles are highly investigated as possible platforms for disease diagnosis and therapy, leading to potential applications in nanomedicine. The state-of-the

Mannosylated brush copolymers based on poly(ethylene glycol) and poly(ε-caprolactone) as multivalent lectin-binding nanomaterials

• Stefania Ordanini,
• Wanda Celentano,
• Anna Bernardi and
• Francesco Cellesi

Beilstein J. Nanotechnol. 2019, 10, 2192–2206, doi:10.3762/bjnano.10.212

• nanoparticles. The ability of these glyco-functionalized nanoparticles to efficiently expose mannose to lectins was investigated with the model Concanavalin A, through turbidity assays. While the monovalent macromolecule was not able to induce Con A clustering, the linear divalent glycopolymer had a binding

Magnetic properties of biofunctionalized iron oxide nanoparticles as magnetic resonance imaging contrast agents

• Natalia E. Gervits,
• Andrey A. Gippius,
• Alexey V. Tkachev,
• Evgeniy I. Demikhov,
• Sergey S. Starchikov,
• Igor S. Lyubutin,
• Alexander L. Vasiliev,
• Vladimir P. Chekhonin,
• Maxim A. Abakumov,
• Alevtina S. Semkina and
• Alexander G. Mazhuga

Beilstein J. Nanotechnol. 2019, 10, 1964–1972, doi:10.3762/bjnano.10.193

• due to the separation of the MNPs by nonmagnetic HSA protein molecules. In particular, this leads to the transition of coated nanoparticles to the paramagnetic state at a lower temperature. The NMR spectra of uncoated, functionalized nanoparticles revealed no changes in the hyperfine parameters of the

Polydopamine-coated Au nanorods for targeted fluorescent cell imaging and photothermal therapy

• Boris N. Khlebtsov,
• Andrey M. Burov,
• Timofey E. Pylaev and
• Nikolai G. Khlebtsov

Beilstein J. Nanotechnol. 2019, 10, 794–803, doi:10.3762/bjnano.10.79

• -functionalized nanoparticles in folate-positive HeLa cells in contrast to the folate-negative HEK 293 cells using fluorescent microscopy. The replacement of folic acid with polyethylene glycol (PEG) leads to a decrease in nanoparticle uptake by both folate-positive and folate-negative cells. We performed NIR

• that folate-functionalized nanoparticles can selectively accumulate in folate-positive HeLa but not in folate-negative HEK 293. The targeted NIR light-mediated phototherapy by using AuNR-PDA-folate showed remarkable cancer cell killing efficiency in vitro in comparison with AuNR-PEG nanoparticles. The

Surface plasmon resonance enhancement of photoluminescence intensity and bioimaging application of gold nanorod@CdSe/ZnS quantum dots

• Siyi Hu,
• Yu Ren,
• Yue Wang,
• Jinhua Li,
• Junle Qu,
• Liwei Liu,
• Hanbin Ma and
• Yuguo Tang

Beilstein J. Nanotechnol. 2019, 10, 22–31, doi:10.3762/bjnano.10.3

• staining appearing in Figure 8 was due to the accumulation of functionalized nanoparticles in the cells, and there was no sign of any damage to the cell, demonstrating passive uptake in MCF-7 breast cancer cells using CdSe/ZnS@FA and GNR@CdSe/ZnS@FA. However, because the PL intensity and cell morphology of

Heavy-metal detectors based on modified ferrite nanoparticles

• Urszula Klekotka,
• Ewelina Wińska,
• Elżbieta Zambrzycka-Szelewa,
• Dariusz Satuła and
• Beata Kalska-Szostko

Beilstein J. Nanotechnol. 2018, 9, 762–770, doi:10.3762/bjnano.9.69

• stirred for 4 h at room temperature. Then, the functionalized nanoparticles were separated from the solutions using a permanent magnet, washed three times with ethanol and left overnight for drying. Modification of nanoparticles with 3-PPA and 16-PHDA Another group of compounds tested and selected for the

The role of ligands in coinage-metal nanoparticles for electronics

• Ioannis Kanelidis and
• Tobias Kraus

Beilstein J. Nanotechnol. 2017, 8, 2625–2639, doi:10.3762/bjnano.8.263

• suggested resulting in films with a conductivity of 4545 S/cm. Assemblies of phosphine- or thiol-functionalized nanoparticles created compact nanoparticle arrangements. Electron transport was dominated by the tunnel barrier imposed by the organic ligands. The conductivities of 0.333 and 0.625 S/cm obtained

Surface-enhanced Raman spectroscopy of cell lysates mixed with silver nanoparticles for tumor classification

• Mohamed Hassoun,
• Iwan W.Schie,
• Tatiana Tolstik,
• Sarmiza E. Stanca,
• Christoph Krafft and
• Juergen Popp

Beilstein J. Nanotechnol. 2017, 8, 1183–1190, doi:10.3762/bjnano.8.120

• immuno-functionalized nanoparticles turned out to be challenging for cell identification because they require complex preparation procedures. Here, a new SERS strategy is presented for cell classification using non-functionalized silver nanoparticles and potassium chloride to induce aggregation. To

• multiplex capability due to narrow band widths. Enhancement of Raman signal of cells can be realized by (1) various techniques of nanoparticles delivery into cells, such as spontaneous uptake, microinjection, electroporation [15][16][17][18][19][20] or (2) binding of antibody-functionalized nanoparticles to

• mixing of solvents and acquisition of SERS spectra, this approach will be transferred to a droplet-based microfluidic lab-on-chip device [24]. After delivery of non-functionalized nanoparticles into cells [20], the SERS approach can also increase the throughput of tumor cell recognition in microfluidic

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• functionalized nanoparticles per cell in the cell culture experiments was calculated accordingly. Cell line culture and imaging Human epithelial cervical cancer cells (HeLa) and human osteosarcoma cells (MG-63) cell lines were cultured in DMEM, supplemented with 10% fetal calf serum (FCS) under 37 °C, 5% CO2 and

• either HTRA1, HTRA2, or BSA. BSA served as a nonfunctional control protein. These protein-functionalized nanoparticles had an almost spherical morphology and a diameter between 30 and 150 nm as shown by SEM (Figure 1). Initially, the functionalized nanoparticles were physicochemically characterized

• dispersion in biological media containing proteins (like RPMI/10% FCS) is much better due to the formation of a protein corona [48][49]. The concentration of the labelled proteins was determined by UV–vis spectroscopy (Table 2). Table 3 gives the quantification data of the functionalized nanoparticles, where

Functionalized TiO₂ nanoparticles by single-step hydrothermal synthesis: the role of the silane coupling agents

• Antoine R. M. Dalod,
• Lars Henriksen,
• Tor Grande and
• Mari-Ann Einarsrud

Beilstein J. Nanotechnol. 2017, 8, 304–312, doi:10.3762/bjnano.8.33

• 700 °C leads to the formation of a nanometric amorphous SiO2 layer, preventing growth and phase transition of the in situ functionalized nanoparticles. Keywords: core–shell nanoparticles; functionalized nanoparticles; hydrothermal synthesis; oriented attachment; silane coupling agent; Introduction

• functionalized nanoparticles with silane coupling agents showed only negligible growth of the crystallites after the heat treatment. Calculated crystallite sizes of the heat-treated samples are listed in Table 2. Additionally, in TiO2-HT, the heat treatment induced a partial phase transition from brookite and

• areas of the heat-treated in situ surface-functionalized nanoparticles and the pure TiO2 samples (Figure S3 of Supporting Information File 1) confirm the presence and the absence of silicon, respectively. Removal of the organic part of the silane coupling agent during the heat treatment induces the

Streptavidin-coated gold nanoparticles: critical role of oligonucleotides on stability and fractal aggregation

• Roberta D'Agata,
• Pasquale Palladino and
• Giuseppe Spoto

Beilstein J. Nanotechnol. 2017, 8, 1–11, doi:10.3762/bjnano.8.1

• adlayer thickness, which is consistent with the contribution of the 11-mer BiotinDNA to the hydrodynamic size of the functionalized nanoparticles [38][39]. The AuNPs hydrodynamic diameter is larger compared to TEM-measured diameter. This evidence, combined with the PDI value of 0.22 ± 0.07 indicating a

• compared to citrate-stabilized AuNPs ζ-potential (Table 1) contributes to improving the stability of functionalized nanoparticles dispersions. Nanoparticles modified with biotinylated oligonucleotides are spontaneously redispersed because of steric and electrostatic repulsion provided by the introduction

• functionalized nanoparticles, the interaction of BiotinDNA with bare AuNPs was also investigated. In fact, the direct interaction of the displaced BiotinDNA with the AuNP surface is to be taken into account if the whole SA-BiotinDNA complex instead of only BiotinDNA desorbs from AuNP-SA-BiotinDNA. The

Antitumor magnetic hyperthermia induced by RGD-functionalized Fe₃O₄ nanoparticles, in an experimental model of colorectal liver metastases

• Oihane K. Arriortua,
• Eneko Garaio,
• Borja Herrero de la Parte,
• Maite Insausti,
• Luis Lezama,
• Fernando Plazaola,
• Jose Angel García,
• Jesús M. Aizpurua,
• Maialen Sagartzazu,
• Mireia Irazola,
• Nestor Etxebarria,
• Ignacio García-Alonso,
• Alberto Saiz-López and
• José Javier Echevarria-Uraga

Beilstein J. Nanotechnol. 2016, 7, 1532–1542, doi:10.3762/bjnano.7.147

• intermediate which could fulfill the “click criteria” to add RGD by means of 1,3-dipolar cycloaddition of azides with the terminal alkynes. The preparation, together with both structural and magnetic characterization of RGD-functionalized nanoparticles, are also presented in this paper. The ultimate goal would

A facile method for the preparation of bifunctional Mn:ZnS/ZnS/Fe₃O₄ magnetic and fluorescent nanocrystals

• Houcine Labiadh,
• Tahar Ben Chaabane,
• Romain Sibille,
• Lavinia Balan and
• Raphaël Schneider

Beilstein J. Nanotechnol. 2015, 6, 1743–1751, doi:10.3762/bjnano.6.178

• clearly confirmed the growth of the Fe3O4 shell around the Mn:ZnS/ZnS core. Note also that these functionalized nanoparticles could be easily redispersed in aqueous medium after purification and drying (Figure 4a). The room temperature, magnetic harvesting of the heterostructured nanocrystals could be

Comparative evaluation of the impact on endothelial cells induced by different nanoparticle structures and functionalization

• Lisa Landgraf,
• Ines Müller,
• Peter Ernst,
• Miriam Schäfer,
• Christina Rosman,
• Isabel Schick,
• Oskar Köhler,
• Hartmut Oehring,
• Vladimir V. Breus,
• Thomas Basché,
• Carsten Sönnichsen,
• Wolfgang Tremel and
• Ingrid Hilger

Beilstein J. Nanotechnol. 2015, 6, 300–312, doi:10.3762/bjnano.6.28

• endothelial cells. These findings are attributed to a rapid internalization of the NH2-functionalized nanoparticles in combination with the damage of intracellular membranes. Interestingly, the endocytotic pathway seems to be a size-dependent process whereas nanoparticles with a size of 20 nm are internalized

• nanoparticles. The uptake rate was normalized as the percentage of cells with nanoparticles. Impact of different shaped and functionalized nanoparticles on the cellular ATP-level of different endothelial cells after 24 h and 72 h of incubation. Relative cellular ATP-levels were detected by ATPLite assay. (a

The fate of a designed protein corona on nanoparticles in vitro and in vivo

• Denise Bargheer,
• Julius Nielsen,
• Gabriella Gébel,
• Markus Heine,
• Sunhild C. Salmen,
• Roland Stauber,
• Horst Weller,
• Joerg Heeren and
• Peter Nielsen

Beilstein J. Nanotechnol. 2015, 6, 36–46, doi:10.3762/bjnano.6.5

• fate of functionalized nanoparticles also in vivo. Results Particle synthesis and characterization Radiolabelling of both the nanoparticle cores and of adsorbed proteins offers a way to follow and quantify the fate of a designed protein corona not only in vitro but also in vivo. For this purpose, we