Supramolecular hydration structure of graphene-based hydrogels: density functional theory, green chemistry and interface application

• Hon Nhien Le,
• Duy Khanh Nguyen,
• Minh Triet Dang,
• Huyen Trinh Nguyen,
• Thi Bang Tam Dao,
• Trung Do Nguyen,
• Chi Nhan Ha Thuc and
• Van Hieu Le

Beilstein J. Nanotechnol. 2025, 16, 806–822, doi:10.3762/bjnano.16.61

• exothermic heat increased the reactor temperature to above 90 °C). After 2 h of agitation, the reaction was mixed with 150 mL of a 5% H2O2 solution and kept stirring for one day. After washing to neutral pH, the material was dried and ground to produce a GrO powder (moisture ≈20%). Next, the GrO powder was

A formulation containing Cymbopogon flexuosus essential oil: improvement of biochemical parameters and oxidative stress in diabetic rats

• Ailton Santos Sena-Júnior,
• Cleverton Nascimento Santana Andrade,
• Pedro Henrique Macedo Moura,
• Jocsã Hémany Cândido dos Santos,
• Cauãn Torres Trancoso,
• Eloia Emanuelly Dias Silva,
• Deise Maria Rego Rodrigues Silva,
• Ênio Pereira Telles,
• Luiz André Santos Silva,
• Isabella Lima Dantas Teles,
• Sara Fernanda Mota de Almeida,
• Daniel Alves de Souza,
• Jileno Ferreira Santos,
• Felipe José Aidar Martins,
• Ana Mara de Oliveira e Silva,
• Sandra Lauton-Santos,
• Guilherme Rodolfo Souza de Araujo,
• Cristiane Bani Correa,
• Rogéria De Souza Nunes,
• Lysandro Pinto Borges and
• Ana Amélia Moreira Lira

Beilstein J. Nanotechnol. 2025, 16, 617–636, doi:10.3762/bjnano.16.48

Zeolite materials with Ni and Co: synthesis and catalytic potential in the selective hydrogenation of citral

• Inocente Rodríguez-Iznaga,
• Yailen Costa Marrero,
• Tania Farias Piñeira,
• Céline Fontaine,
• Lexane Paget,
• Beatriz Concepción Rosabal,
• Arbelio Penton Madrigal,
• Vitalii Petranovskii and
• Gwendoline Lafaye

Beilstein J. Nanotechnol. 2025, 16, 520–529, doi:10.3762/bjnano.16.40

• SEM image (Figure 2). To remove excess solution, water washes are typically applied. The materials obtained via IE underwent extensive washing with distilled water, while those obtained via Imp were only lightly washed, leading to the observed differences in chloride content. According to this, there

• for materials obtained by IE are higher than those of ZSA. This increase is consistent with expectations [17][18], as the ion exchange and water washing treatments effectively clean the materials’ surface, thereby enhancing the available surface area and porosity (microporosity) for adsorption. The

N₂⁺-implantation-induced tailoring of structural, morphological, optical, and electrical characteristics of sputtered molybdenum thin films

• Usha Rani,
• Kafi Devi,
• Divya Gupta and
• Sanjeev Aggarwal

Beilstein J. Nanotechnol. 2025, 16, 495–509, doi:10.3762/bjnano.16.38

• minimizing the reflected power. Before deposition, the target surface was pre-sputtered for 15 min to remove any surface contamination. The silicon substrates were meticulously cleaned by washing them with distilled water and isopropyl alcohol and rinsing them with acetone. The vacuum chamber was evacuated

Quantification of lead through rod-shaped silver-doped zinc oxide nanoparticles using an electrochemical approach

• Ravinder Lamba,
• Gaurav Bhanjana,
• Neeraj Dilbaghi,
• Vivek Gupta and
• Sandeep Kumar

Beilstein J. Nanotechnol. 2025, 16, 422–434, doi:10.3762/bjnano.16.33

• 2854 cm−1 relate to the stretching vibration of carbon and hydrogen bonds. The peaks at 1030 and 1380 cm−1 are related to vibrational or in-plane bending of residual ethanol which was used for washing the nanoparticles and KBr pellet die set (used for pellet making for FTIR) [18][19]. Optical study of

• stirring for 2 h at room temperature. Once the stirring was complete, the deposited precipitate was formed. These precipitates were subjected to centrifugation and the pellet was collected; further washing was done multiple times using distilled water and ethanol. The obtained nanoparticles were oven-dried

Development of a mucoadhesive drug delivery system and its interaction with gastric cells

• Ahmet Baki Sahin,
• Serdar Karakurt and
• Deniz Sezlev Bilecen

Beilstein J. Nanotechnol. 2025, 16, 371–384, doi:10.3762/bjnano.16.28

• interaction were 4.5 ± 2.7 and 7.4 ± 0.3 for 6 × 104 and 1.2 × 105 cells/well, respectively. As expected, the nanoparticle adsorption onto the cells depends on the cell concentration. Since the fluorescence measurements were carried out on the cell pellets after washing and centrifugation, the increase in

• administration (control A) and (ii) empty wells with nanoparticle administration (control B). The relative mucus interaction was calculated through a formula similar to that used in other studies [77]: Here, F1 is the fluorescence obtained from the cell pellet treated with NPs after incubation, washing, and

• centrifugation. F1′ is the fluorescence obtained from the cell pellet of control A. F0 is the fluorescence of nanoparticles administered onto the cells (input). Fx is the fluorescence obtained from control B after incubation, washing, and centrifugation. And F0x is the fluorescence of nanoparticles administered

Graphene oxide–chloroquine conjugate induces DNA damage in A549 lung cancer cells through autophagy modulation

• Braham Dutt Arya,
• Sandeep Mittal,
• Prachi Joshi,
• Alok Kumar Pandey,
• Jaime E. Ramirez-Vick,
• Govind Gupta and
• Surinder P. Singh

Beilstein J. Nanotechnol. 2025, 16, 316–332, doi:10.3762/bjnano.16.24

• were collected using successive centrifugation steps at 13,000 rpm for 30 min and washing with deionized (DI) water several times, until the hydrolysis of covalent sulfates formed during oxidation [26][27]. The final product of exfoliated GO nanosheets were extracted through freeze-drying. Binding of

• factors such as the source of graphite, the weight equivalent of KMnO4, reaction time, and washing conditions [26][37]. Most importantly, the degree of oxidation of graphene oxide plays a crucial role in controlling the cytotoxicity of the material [38][39]. To achieve higher oxidation, six times weight

Instance maps as an organising concept for complex experimental workflows as demonstrated for (nano)material safety research

• Benjamin Punz,
• Maja Brajnik,
• Joh Dokler,
• Jaleesia D. Amos,
• Litty Johnson,
• Katie Reilly,
• Anastasios G. Papadiamantis,
• Amaia Green Etxabe,
• Lee Walker,
• Diego S. T. Martinez,
• Steffi Friedrichs,
• Klaus M. Weltring,
• Nazende Günday-Türeli,
• Claus Svendsen,
• Christine Ogilvie Hendren,
• Mark R. Wiesner,
• Martin Himly,
• Iseult Lynch and
• Thomas E. Exner

Beilstein J. Nanotechnol. 2025, 16, 57–77, doi:10.3762/bjnano.16.7

• supernatant upon several washing steps. “In Vitro Sedimentation, Diffusion and Dosimetry” studies were undertaken to determine the cell-delivered dose for all culture conditions based on the specific density and size parameters of the bio-nano conjugates [52]. Finally, comprehensive physicochemical

Bioinspired nanofilament coatings for scale reduction on steel

• Siad Dahir Ali,
• Mette Heidemann Rasmussen,
• Jacopo Catalano,
• Christian Husum Frederiksen and
• Tobias Weidner

Beilstein J. Nanotechnol. 2025, 16, 25–34, doi:10.3762/bjnano.16.3

• , and dried after the first three hours of coating. When the coating was finished after a total of six hours, the samples were washed in toluene and left in Milli-Q water for approximately 12 hours. The last washing step ensures that all produced HCl is removed from the coating which will otherwise

Attempts to preserve and visualize protein corona on the surface of biological nanoparticles in blood serum using photomodification

• Julia E. Poletaeva,
• Anastasiya V. Tupitsyna,
• Alina E. Grigor’eva,
• Ilya S. Dovydenko and
• Elena I. Ryabchikova

Beilstein J. Nanotechnol. 2024, 15, 1654–1666, doi:10.3762/bjnano.15.130

• and washing (90 min with UC at 100,000g) deprived photomodified bio-NPs of the ability to preserve the protein corona. The study presented in this article is a pioneering one. We used the photomodification method that we developed earlier, which allowed us to obtain and visualize a complete protein

Ultrablack color in velvet ant cuticle

• Vinicius Marques Lopez,
• Wencke Krings,
• Juliana Reis Machado,
• Stanislav Gorb and
• Rhainer Guillermo-Ferreira

Beilstein J. Nanotechnol. 2024, 15, 1554–1565, doi:10.3762/bjnano.15.122

• –2.00 hPa. For sample preparation, transversely sectioned T. bifurca specimens were fixed in Karnovsky fixative solution + 0.1% ruthenium red for 12 h. Following buffer washing, a post-fixative solution (osmium tetroxide + 0.1% ruthenium red) was applied at room temperature. After rinsing with alcohol

Green synthesis of carbon dot structures from Rheum Ribes and Schottky diode fabrication

• Muhammed Taha Durmus and
• Ebru Bozkurt

Beilstein J. Nanotechnol. 2024, 15, 1369–1375, doi:10.3762/bjnano.15.110

• substrate was cut to approximately 1 cm2 in size and cleaned by washing it in acetone, methanol, and pure water for 10 min each. An ohmic contact was made with aluminum on the cleaned sample at 1 × 10−7 Torr vacuum in a PVD thermal evaporation device. Approximately 30 μL of the CDs stock solution was taken

Nanoarchitectonics with cetrimonium bromide on metal nanoparticles for linker-free detection of toxic metal ions and catalytic degradation of 4-nitrophenol

• Akash Kumar and
• Raja Gopal Rayavarapu

Beilstein J. Nanotechnol. 2024, 15, 1312–1332, doi:10.3762/bjnano.15.106

• samples, nearly the same amount of CTAB is present. In addition to this, the as-prepared CTAB-AuNR1 and CTAB-AuNR2 were also evaluated regarding heavy metal detection. Although the nanoparticle concentration has a big impact, washing nanoparticles is crucial in detecting heavy metals. As-prepared

• nanoparticles of the same concentration and size were evaluated to understand the impact of washing. As-prepared AuNR1 at 0.5 and 1 OD did not detect any metal through significant changes in color or plasmon resonance (Supporting Information File 1, Figure S15 and Figure S16). However, a slight blueshift of as

Dual-functionalized architecture enables stable and tumor cell-specific SiO₂NPs in complex biological fluids

• Iris Renata Sousa Ribeiro,
• Raquel Frenedoso da Silva,
• Romênia Ramos Domingues,
• Adriana Franco Paes Leme and
• Mateus Borba Cardoso

Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100

• DMEM culture medium (10% FBS) at 37 °C for 10 min, using the thermoblock Thermomixer C (Eppendorf). Posteriorly, the precipitate was washed with PBS (pH 7.4, 10 mM) to remove excess proteins weakly adsorbed on SiO2NPs. The washing procedure was performed by centrifugation (14000 rpm for 10 min at 4 °C

• ) and ii) human plasma (5.5% in PBS) at 37 °C for 10 min, using the thermoblock Thermomixer C (Eppendorf). After this period, the precipitate was washed with PBS (pH 7.4, 10 mM) to remove excess proteins weakly adsorbed on the surface of SiO2NPs. The washing procedure was performed by centrifugation

Effect of wavelength and liquid on formation of Ag, Au, Ag/Au nanoparticles via picosecond laser ablation and SERS-based detection of DMMP

• Sree Satya Bharati Moram,
• Chandu Byram and
• Venugopal Rao Soma

Beilstein J. Nanotechnol. 2024, 15, 1054–1069, doi:10.3762/bjnano.15.86

• and cut into 1 cm × 1 cm pieces. The targets were thoroughly cleaned in an ultrasonic bath using ethanol, acetone, and DW for 10 min. After washing, the targets were fixed at the bottom of the glass beaker filled with 5 mL of DW and aqueous NaCl solution (1 mM). The setup was mounted on the motorized

Radiofrequency enhances drug release from responsive nanoflowers for hepatocellular carcinoma therapy

• Yanyan Wen,
• Ningning Song,
• Yueyou Peng,
• Weiwei Wu,
• Qixiong Lin,
• Minjie Cui,
• Rongrong Li,
• Qiufeng Yu,
• Sixue Wu,
• Yongkang Liang,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2024, 15, 569–579, doi:10.3762/bjnano.15.49

• @MnO2 NFs The CUR-Fe@MnO2 NFs uptake by Huh-7 cells was observed under a fluorescence microscope. Huh-7 cells were incubated with 50 µg/mL NFs for 24 h. Then, 4% paraformaldehyde was used to fix the Huh-7 cells for 15 min, followed by washing with PBS. Fixed cells were stained by the Prussian Blue kit

Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent

• Horacio Emanuel Jerez,
• Yamila Roxana Simioni,
• Kajal Ghosal,
• Maria Jose Morilla and
• Eder Lilia Romero

Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46

• HUVECs were seeded and incubated with nanovesicles as stated before. After incubation, supernatants were discarded, and cells were washed once with 100 µL PBS and incubated with 2.5 μg/mL JC-1 staining mixture for 15 min at 37 °C. Upon supernatant removal and PBS washing, the fluorescence intensity of

Vinorelbine-loaded multifunctional magnetic nanoparticles as anticancer drug delivery systems: synthesis, characterization, and in vitro release study

• Zeynep Özcan and
• Afife Binnaz Hazar Yoruç

Beilstein J. Nanotechnol. 2024, 15, 256–269, doi:10.3762/bjnano.15.24

• amounts: 100, 200, and 400 mg; dopamine hydrochloride/Fe3O4 ratios: 1:1, 2:1, and 4:1) and stirred at room temperature for 15 h at 1000 rpm. Following this stage, the obtained PDA-coated Fe3O4 NPs were separated from the solution using a magnet. Subsequently, unreacted material was removed by washing

• particles were removed by washing with distilled water. The PEGylation PDA/Fe3O4 NPs were then dried in a vacuum oven at 45 °C. The PDA-coated Fe3O4 nanoparticles were modified with SH-PEG to facilitate their accumulation in tumour regions. In similar studies, the conjugation of SH-PEG onto the surface of

Study of the reusability and stability of nylon nanofibres as an antibody immobilisation surface

• Inés Peraile,
• Matilde Gil-García,
• Laura González-López,
• Nushin A. Dabbagh-Escalante,
• Juan C. Cabria-Ramos and
• Paloma Lorenzo-Lozano

Beilstein J. Nanotechnol. 2024, 15, 83–94, doi:10.3762/bjnano.15.8

• immobilized on the surface of the NFs containing protein A/G through 1 h of incubation. Then, RPE-labelled BSA (10 µL 100 µg/mL in blocking buffer) was immunocaptured by the anchored antibodies over a period of 1 h. Washing steps were carried out between each step in order to remove non-linked excess reagents

• (immediately after antibody anchoring; it is considered the reference value) and 7 days, 30 days, and 90 days after antibody anchoring, bringing the immunocapture systems to room temperature only covered by aluminum foil. Washing steps were carried out between each step above in order to remove non-linked

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• ). After washing three times with distilled water and adding 5 mL of water, an aliquot of 0.5 mL of particle suspension (a volume concentration of 8%) was then incubated with 2 mg/mL CUR in DMSO (final concentration of CUR and DMSO were 1 mg/mL and 50% (v/v), respectively) for 1 h at RT and protected from

Recognition mechanisms of hemoglobin particles by monocytes – CD163 may just be one

• Jonathan-Gabriel Nimz,
• Pichayut Rerkshanandana,
• Chiraphat Kloypan,
• Ulrich Kalus,
• Saranya Chaiwaree,
• Axel Pruß,
• Radostina Georgieva,
• Yu Xiong and
• Hans Bäumler

Beilstein J. Nanotechnol. 2023, 14, 1028–1040, doi:10.3762/bjnano.14.85

• with the particles for 5 min. After washing in 0.9% NaCl solution, particles were centrifuged (3000g, 3 min), and the supernatant was decanted. Particles were then resuspended in 0.9% NaCl. For crosslinking, 0.02% glutaraldehyde (GA) was added to the particle suspension, which was then incubated for 1

• h at room temperature under stirring. After another washing in aqua dest. the MnCO3 matrix was dissolved using 0.25 M EDTA. After 30 min incubation time, 0.2 mg/mL NaBH4 in 0.1 M NaOH was added to prevent oxidation of Hb to met-Hb. Incubation for another 30 min followed. After triplicate washing in

• a washing solution of 0.9% NaCl containing 0.2% HSA, particles were resuspended in 0.9% NaCl, checked via light microscopy for aggregation, and stored at 4 °C. Particle characterization To ensure that the subsequent experiments would be carried out on particles that also met the quality requirements

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

• Brunno R. F. Verçoza,
• Robson R. Bernardo,
• Luiz Augusto S. de Oliveira and
• Juliany C. F. Rodrigues

Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73

• passed when they reached confluence in the bottles. In addition, primary cultures of murine macrophages were obtained from the peritoneal cavity of CF1 mice by washing with Hanks’ balanced solution. Then, they were plated on coverslips in a 24-well culture plate and placed to adhere for 24 h at 37 °C in

Industrial perspectives for personalized microneedles

• Remmi Danae Baker-Sediako,
• Benjamin Richter,
• Matthias Blaicher,
• Michael Thiel and
• Martin Hermatschweiler

Beilstein J. Nanotechnol. 2023, 14, 857–864, doi:10.3762/bjnano.14.70

• on a “Quantum X shape” lithography system (Nanoscribe GmbH & Co, Karlsruhe, Germany) following printing and post-printing protocols from NanoGuide. Post-printing protocols begin by removing unpolymerized material via two-step washing with either propylene glycol methyl ether acetate or mr-Dev for 15

In situ magnesiothermic reduction synthesis of a Ge@C composite for high-performance lithium-ion batterie anodes

• Ha Tran Huu,
• Ngoc Phi Nguyen,
• Vuong Hoang Ngo,
• Huy Hoang Luc,
• Minh Kha Le,
• Minh Thu Nguyen,
• My Loan Phung Le,
• Hye Rim Kim,
• In Young Kim,
• Sung Jin Kim,
• Van Man Tran and
• Vien Vo

Beilstein J. Nanotechnol. 2023, 14, 751–761, doi:10.3762/bjnano.14.62

• ) water, before being dried in a vacuum oven at 110 °C for 12 h. The product was further heated under air at 300 °C for 3 h. The activated carbon was obtained after washing with 2 M HCL and DI water. It was dried under vacuum and denoted as BC-800. In situ fabrication of Ge/C-iM750 composite material A

A graphene quantum dots–glassy carbon electrode-based electrochemical sensor for monitoring malathion

• Sanju Tanwar,
• Aditi Sharma and
• Dhirendra Mathur

Beilstein J. Nanotechnol. 2023, 14, 701–710, doi:10.3762/bjnano.14.56

• the 40 mL suspension at 200 °C for 5 h in a 50 mL poly(tetrafluoroethylene)-lined autoclave. Washing with DI water was carried out in order to remove the acid from the resulting black suspension once it had been cooled to room temperature. NaOH solution was subsequently used to neutralize the solid