Prospects of nanotechnology and natural products for cancer and immunotherapy

• Jan Filipe Andrade Santos,
• Marcela Bernardes Brasileiro,
• Pamela Danielle Cavalcante Barreto,
• Ligiane Aranha Rocha and
• José Adão Carvalho Nascimento Júnior

Beilstein J. Nanotechnol. 2025, 16, 1644–1667, doi:10.3762/bjnano.16.116

• scattering (DLS), and UV–visible (UV–vis) spectroscopy were used for characterization. The nanoparticles showed increased cellular uptake compared to free PD-L1, suppression of the NF-κB pathway as indicated by reduced PHO-P65 protein expression, and enhanced tumor inhibition due to immune activation and

Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion

• Philippe de Castro Mesquita,
• Karla Samara Rocha Soares,
• Manoela Torres-Rêgo,
• Emanuell dos Santos-Silva,
• Mariana Farias Alves-Silva,
• Alianda Maira Cornélio,
• Matheus de Freitas Fernandes-Pedrosa and
• Arnóbio Antônio da Silva-Júnior

Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115

• polyethylenimine for loading peptides and proteins of T. serrulatus venom, and their use as a potential immunoadjuvant was evaluated. The protein loading efficiency of about 100% and the polyacrylamide gel electrophoresis assay confirmed the success of venom loading. Dynamic light scattering and zeta potential

• shape, uniform aspects, and sizes compatible with the results obtained by dynamic light scattering. These morphological features are known to influence the biological activity of nanoparticles loaded with bioactive molecules, directly affecting their release. The release profile of a substance depends

• , United States of America), equipped with a 90 Plus/BI-MAS apparatus, at a wavelength of 659 nm with a scattering angle of 90°. For the analysis, 100 μL of the nanoparticle suspension was diluted in 900 μL of deionized water (1:10 dilution) to ensure dispersions within a suitable experimental range (100

Influence of laser beam profile on morphology and optical properties of silicon nanoparticles formed by laser ablation in liquid

• Natalie Tarasenka,
• Vladislav Kornev,
• Alena Nevar and
• Nikolai Tarasenko

Beilstein J. Nanotechnol. 2025, 16, 1533–1544, doi:10.3762/bjnano.16.108

• directions depending on the material and laser parameters. As shown previously [33], contrary to conventional optical traps using Gaussian beams, Bessel beam traps exhibit significant gradients only in the transverse directions, which leads to particle confinement in two dimensions with a scattering force in

Laser processing in liquids: insights into nanocolloid generation and thin film integration for energy, photonic, and sensing applications

• Akshana Parameswaran Sreekala,
• Pooja Raveendran Nair,
• Jithin Kundalam Kadavath,
• Bindu Krishnan,
• David Avellaneda Avellaneda,
• M. R. Anantharaman and
• Sadasivan Shaji

Beilstein J. Nanotechnol. 2025, 16, 1428–1498, doi:10.3762/bjnano.16.104

• mechanism of NPs in PLAL has been investigated extensively, primarily through the use of laser-induced fluorescence analysis and shadowgraph analysis [17][18][19][20][21]. Use of small-angle X-ray scattering, wide-angle X-ray scattering, and X-ray imaging techniques has enabled a more comprehensive

Photochemical synthesis of silver nanoprisms via green LED irradiation and evaluation of SERS activity

• Tuan Anh Mai-Ngoc,
• Nhi Kieu Vo,
• Cong Danh Nguyen,
• Thi Kim Xuan Nguyen and
• Thanh Sinh Do

Beilstein J. Nanotechnol. 2025, 16, 1417–1427, doi:10.3762/bjnano.16.103

• candidates for surface-enhanced Raman scattering (SERS) due to their strong localized surface plasmon resonance and sharp tip geometry. In this study, AgNPrs were synthesized through a photochemical method by irradiating spherical silver nanoparticle seeds with 10 W green light-emitting diodes (LEDs; 520

• applications. Keywords: light-emitting diodes (LEDs); photochemical synthesis; silver nanoprisms; surface-enhanced Raman scattering (SERS); trisodium citrate; Introduction Anisotropic silver nanoparticles (ASNPs) have attracted increasing attention from research groups worldwide due to their potential

• applications in optical sensing, particularly in surface-enhanced Raman scattering (SERS) [1]. Among ASNPs, silver nanoprisms (AgNPrs) are of particular interest because of their broad absorption in the visible range (400–900 nm), enabling them to display a wide spectrum of colors such as yellow, red, orange

Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems

• Alejandro Llamedo,
• Marina Cano,
• Raquel G. Soengas and
• Francisco J. García-Alonso

Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98

• by dynamic light scattering. The antibacterial activity of these nanoparticles was evaluated against Gram-positive and Gram-negative bacterial strains (S. aureus, S. epidermidis, E. coli, and P. aeruginosa) and compared to the free complex 18. While neither free drug 18 nor the encapsulated

Ferroptosis induction by engineered liposomes for enhanced tumor therapy

• Alireza Ghasempour,
• Mohammad Amin Tokallou,
• Mohammad Reza Naderi Allaf,
• Mohsen Moradi,
• Hamideh Dehghan,
• Mahsa Sedighi,
• Mohammad-Ali Shahbazi and
• Fahimeh Lavi Arab

Beilstein J. Nanotechnol. 2025, 16, 1325–1349, doi:10.3762/bjnano.16.97

• light scattering (DLS) is commonly used to determine liposome size and size distribution. Transmission electron microscopy (TEM) and atomic force microscopy (AFM) can be used to image liposome morphology and determine lamellarity. Zeta potential measurements assess the surface charge of liposomes, which

• liposomes is preserved [110]. The surface charge of liposomes, often expressed as zeta potential, is an important parameter that influences their stability and interaction with biological systems. Electrophoretic light scattering is often used to measure the zeta potential of liposomes [110][125]. Cryo-TEM

Wavelength-dependent correlation of LIPSS periodicity and laser penetration depth in stainless steel

• Nitin Chaudhary,
• Chavan Akash Naik,
• Shilpa Mangalassery,
• Jai Prakash Gautam and
• Sri Ram Gopal Naraharisetty

Beilstein J. Nanotechnol. 2025, 16, 1302–1315, doi:10.3762/bjnano.16.95

• density coherently oscillates, coupled to both media. Usually, LIPSS formation is a multipulse phenomenon, as pulse after pulse create a different roughness on the surface or feedback mechanism to form certain SPP modes. The interference of incoming light with scattering from the SPP modes can lead to the

• absorption in the metal and scattering in other directions, which depend on the properties of the metal [43]. The penetration depth of SPPs into the metal measures how far the electric field of the SPP decays exponentially inside the metal. Ash and colleagues reported the impact of wavelength and beam width

Enhancing the photoelectrochemical performance of BiOI-derived BiVO₄ films by controlled-intensity current electrodeposition

• Huu Phuc Dang,
• Khanh Quang Nguyen,
• Nguyen Thi Mai Tho and
• Tran Le

Beilstein J. Nanotechnol. 2025, 16, 1289–1301, doi:10.3762/bjnano.16.94

• . Larger grains typically result in fewer grain boundaries, reducing phonon scattering and enhancing vibrational coherence. Raman spectra also provide insights into the influence of fabrication conditions on surface chemistry. The relative intensities of the peaks suggest that higher deposition currents

• spectrum, (b) bandgap energies of BiVO4(146), BiVO4(224), BiVO4(226), BiVO4(324), and BiVO4(326) photoanodes. Minor fluctuations above 500 nm are due to light scattering from porous films. Raman spectrum of BiVO4(146), BiVO4(224), BiVO4(226), BiVO4(324), and BiVO4(326) photoanodes. (a) LSV curves, (b) ABPE

Acrocomia aculeata oil-loaded nanoemulsion: development, anti-inflammatory properties, and cytotoxicity evaluation

• Verónica Bautista-Robles,
• Hady Keita,
• Edgar Julián Paredes Gamero,
• Layna Tayná Brito Leite,
• Jessica de Araújo Isaías Muller,
• Mônica Cristina Toffoli Kadri,
• Ariadna Lafourcade Prada and
• Jesús Rafael Rodríguez Amado

Beilstein J. Nanotechnol. 2025, 16, 1277–1288, doi:10.3762/bjnano.16.93

• , maintaining consistent zeta potential and droplet size parameters over a 180-day storage period at 25 ± 2 °C. Dynamic light scattering analysis revealed a mean nanodroplet size (by intensity) of 173.6 ± 0.70 nm (Figure 2A). The nanoemulsion, composed of 0.28 parts of Span 80® and 0.72 parts of Tween 80

• the smallest droplet size was selected as the (HLBr) to emulsify bocaiúva oil [63]. Droplet size and zeta potential Droplet size and polydispersity index (PDI) were measured by dynamic light scattering (DLS) with a Zetasizer Nano-ZS instrument (Malvern, UK). Zeta potential was determined by

• electrophoretic light scattering with a Zetasizer Nano-ZS instrument (Malvern, UK). AANE was diluted with Milli-Q water (1:25, v/v). All measurements were performed in triplicate, and results were presented as the mean ± standard deviation [42]. Shelf stability The selected AANE was transferred to an amber vial

Investigation of the solubility of protoporphyrin IX in aqueous and hydroalcoholic solvent systems

• Michelly de Sá Matsuoka,
• Giovanna Carla Cadini Ruiz,
• Marcos Luciano Bruschi and
• Jéssica Bassi da Silva

Beilstein J. Nanotechnol. 2025, 16, 1209–1215, doi:10.3762/bjnano.16.89

• , complementary methods may be performed together, such as dynamic light scattering [26] and turbidimetry [27]. Furthermore, TEM analysis can provide a more accurate understanding of the incorporation of PpIX into the micellar systems, overcoming the limitations associated with spectrophotometric estimations

• . These limitations include potential overestimation of solubility due to light scattering by aggregates, turbidity-related interference, and the inability to distinguish between molecularly dissolved and aggregated species [28]. Therefore, TEM micrographs of PpIX samples in different media containing

Chitosan nanocomposite containing rotenoids: an alternative bioinsecticidal approach for the management of Aedes aegypti

• Maria A. A. Bertonceli,
• Vitor D. C. Cristo,
• Ivo J. Vieira,
• Francisco J. A. Lemos,
• Arnoldo R. Façanha,
• Raimundo Braz-Filho,
• Gustavo V. T. Batista,
• Luis G. M. Basso,
• Sérgio H. Seabra,
• Thalya S. R. Nogueira,
• Felipe F. Moreira,
• Arícia L. E. M. Assis,
• Antônia E. A. Oliveira and
• Kátia V. S. Fernandes

Beilstein J. Nanotechnol. 2025, 16, 1197–1208, doi:10.3762/bjnano.16.88

• , controls), while the other corresponded to nanocomposites containing rotenoids (CS/TPP rot and CS/TPP–β-CD rot). Size, polydispersity, and zeta potential The average size (in nm) and PDI of the nanoparticles were measured using dynamic light scattering (DLS) on the Litesizer DLS 500 instrument (Anton Paar

Mechanical stability of individual bacterial cells under different osmotic pressure conditions: a nanoindentation study of Pseudomonas aeruginosa

• Lizeth García-Torres,
• Idania De Alba Montero,
• Eleazar Samuel Kolosovas-Machuca,
• Facundo Ruiz,
• Sumati Bhatia,
• Jose Luis Cuellar Camacho and
• Jaime Ruiz-García

Beilstein J. Nanotechnol. 2025, 16, 1171–1183, doi:10.3762/bjnano.16.86

• . Using forward light scattering experiments, Çetiner et al. reported that the time required for PA to reach an equilibrium state after osmolyte release when exposed to drastic osmotic downshocks was about 150 ms [14]. In our experiments, PA was tested in different conditions after the solution was

Crystalline and amorphous structure selectivity of ignoble high-entropy alloy nanoparticles during laser ablation in organic liquids is set by pulse duration

• Robert Stuckert,
• Felix Pohl,
• Oleg Prymak,
• Ulrich Schürmann,
• Christoph Rehbock,
• Lorenz Kienle and
• Stephan Barcikowski

Beilstein J. Nanotechnol. 2025, 16, 1141–1159, doi:10.3762/bjnano.16.84

Towards a quantitative theory for transmission X-ray microscopy

• James G. McNally,
• Christoph Pratsch,
• Stephan Werner,
• Stefan Rehbein,
• Andrew Gibbs,
• Jihao Wang,
• Thomas Lunkenbein,
• Peter Guttmann and
• Gerd Schneider

Beilstein J. Nanotechnol. 2025, 16, 1113–1128, doi:10.3762/bjnano.16.82

• equations for a sphere of given diameter and homogeneous refractive index n = 1 – δ + iβ [22], an approach well suited to the spherical nanoparticles examined here. Mie theory is one example of scattering theory in which the time-independent part of the final field is given by the sum of the incident and

• scattered fields: At a sufficient distance from the scattering object (in our case, the distance between the nanosphere and the zone plate), the scattered field is well approximated by a spherical wave, and can be written as: where S is the scattering amplitude, ψ the scattering angle from the sphere to a

• problem reduces to determination of the scattering amplitude S(ψ). The Mie solution S(ψ) is given by an infinite series of vector spherical harmonics that for numerical computations is truncated based on the accuracy required. Conventional Mie theory is based on plane waves parallel to the optical axis

Influence of ion beam current on the structural, optical, and mechanical properties of TiO₂ coatings: ion beam-assisted vs conventional electron beam evaporation

• Agata Obstarczyk and
• Urszula Wawrzaszek

Beilstein J. Nanotechnol. 2025, 16, 1097–1112, doi:10.3762/bjnano.16.81

• showed that the annealed film was composed of big, elongated grains with a length from 160 to 350 nm (Figure 3a). It is worth noting that the coatings had a milky color. This was probably the result of the size of grains, which could lead to a significant scattering of light. In contrast, the use of the

• high refractive index and thickness up to 400 nm are required. Although the density and the refractive index of crystalline TiO2 are higher than those of the amorphous phase, it is often preferred in optical devices due to its lower surface roughness and light scattering [65]. Nanoindentation

Fabrication of metal complex phthalocyanine and porphyrin nanoparticle aqueous colloids by pulsed laser fragmentation in liquid and their potential application to a photosensitizer for photodynamic therapy

• Taisei Himeda,
• Risako Kunitomi,
• Ryosuke Nabeya,
• Tamotsu Zako and
• Tsuyoshi Asahi

Beilstein J. Nanotechnol. 2025, 16, 1088–1096, doi:10.3762/bjnano.16.80

• within the nanoparticles. The hydrodynamic diameter of the colloidal dispersion was examined by dynamic light scattering (DLS) measurements, and the Z-average value was 96 nm (see Figure S2, Supporting Information File 1). The fabricated nanoparticles remained stably dispersed over a period of one month

• microcrystalline powder into small nanoparticles with high efficiency. The particle size was determined by dynamic light scattering measurements (Zetasizer nanoS, Malvern Instruments). In this analysis, the prepared colloids were diluted more than tenfold with ion-exchanged water. Dispersion stability Dispersion

Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

• Sevin Adiguzel,
• Nilay Cicek,
• Zehra Cobandede,
• Feray B. Misirlioglu,
• Hulya Yilmaz and
• Mustafa Culha

Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78

• -Vis-NIR) measured optical properties across the 200–800 nm range, while dynamic light scattering (DLS, Malvern Panalytical) provided data on hydrodynamic size and zeta potential after dispersing 1 mg of material in 1 mL of deionized water and sonicating for 30 min. Finally, piezoresponse force

Soft materials nanoarchitectonics: liquid crystals, polymers, gels, biomaterials, and others

• Katsuhiko Ariga

Beilstein J. Nanotechnol. 2025, 16, 1025–1067, doi:10.3762/bjnano.16.77

• (Figure 5) [237]. The compounds exhibited ferroelectric chiral smectic C, and ordered smectic phases. The purpose of this research was to examine how these chiral units contribute to electrical functions. A specific, non-scattering photocurrent decrease was observed in the organized smectic phase of

A calix[4]arene-based supramolecular nanoassembly targeting cancer cells and triggering the release of nitric oxide with green light

• Cristina Parisi,
• Loredana Ferreri,
• Tassia J. Martins,
• Francesca Laneri,
• Samantha Sollima,
• Antonina Azzolina,
• Antonella Cusimano,
• Nicola D’Antona,
• Grazia M. L. Consoli and
• Salvatore Sortino

Beilstein J. Nanotechnol. 2025, 16, 1003–1013, doi:10.3762/bjnano.16.75

• typical features of the ABF chromophore [55][56][57][58][59][62][63][64] with a maximum at ca. 480 nm (Figure 1A). However, dynamic light scattering (DLS) measurements evidenced that, in line with its amphiphilic character, compound 1 is not present in the monomeric form but as nanoaggregates ca. 180 nm

• equation: Dynamic light scattering measurements were performed on a ZetaSizer NanoZS90 (Malvern Instrument, UK), equipped with a 633 nm laser, at a scattering angle of 90° and at 25 °C. In a manner analogous to [70], photolysis experiments were performed by irradiating the samples in solution in a

Time-resolved probing of laser-induced nanostructuring processes in liquids

• Maximilian Spellauge,
• David Redka,
• Mianzhen Mo,
• Changyong Song,
• Heinz Paul Huber and
• Anton Plech

Beilstein J. Nanotechnol. 2025, 16, 968–1002, doi:10.3762/bjnano.16.74

• scattering; Review Introduction Laser synthesis and processing of materials with emphasis on structure formation on the nanoscale offers a multitude of pathways to desired structure-related functions [1]. In green processes, nanostructures and nanoparticles (NPs) can be produced that serve applications in

• verifying proposed pathways, we describe spatially and temporally resolved optical reflectivity and short-wavelength scattering methods that are able to directly reveal structural motifs. Ultimately, it is possible to investigate single NPs with a single shot before complete destruction. With these methods

• -particle imaging” describes the processes initiated by laser-excitation of an individual NP probed by X-ray scattering up to delay times of a few tens of picoseconds. The second chapter “Structural dynamics in liquids” reports on the application of ultrafast time-resolved electron scattering to investigate

Characterization of ion track-etched conical nanopores in thermal and PECVD SiO₂ using small angle X-ray scattering

• Shankar Dutt,
• Rudradeep Chakraborty,
• Christian Notthoff,
• Pablo Mota-Santiago,
• Christina Trautmann and
• Patrick Kluth

Beilstein J. Nanotechnol. 2025, 16, 899–909, doi:10.3762/bjnano.16.68

• nanopores in thermal and plasma-enhanced chemical vapor-deposited (PECVD) SiO2 using synchrotron-based small-angle X-ray scattering (SAXS). The nanopores were fabricated by irradiating the samples with 89 MeV, 185 MeV, and 1.6 GeV Au ions, followed by hydrofluoric acid etching. We present a new approach for

• nanopores in different materials, which is essential for optimizing membrane performance in applications that require precise pore geometry. Keywords: etched ion tracks; SiO2; small angle X-ray scattering (SAXS); swift heavy ion irradiation; track-etched nanopores; Introduction Solid-state nanopores have

• , including selectivity, throughput, and molecular capture. Small-angle X-ray scattering (SAXS) has proven to be an invaluable tool for characterizing nanopore membranes, offering nondestructive analytical capabilities that yield statistical information of more than 106 pores [6][13][29][40]. With a beam size

Heat-induced transformation of nickel-coated polycrystalline diamond film studied in situ by XPS and NEXAFS

• Olga V. Sedelnikova,
• Yuliya V. Fedoseeva,
• Dmitriy V. Gorodetskiy,
• Yuri N. Palyanov,
• Elena V. Shlyakhova,
• Eugene A. Maksimovskiy,
• Anna A. Makarova,
• Lyubov G. Bulusheva and
• Aleksandr V. Okotrub

Beilstein J. Nanotechnol. 2025, 16, 887–898, doi:10.3762/bjnano.16.67

• (Figure 5a, Supporting Information File 1, Figure S5a,b). They demonstrate the main diamond peak at 1333 cm−1 corresponding to the first-order scattering of the F2g symmetry. The high intensity and small full width at half maximum (FWHM) of 4 cm−1 of this band and the absence of other Raman features

• the annealed Ni-PCD film only demonstrate the Raman peaks from sp2-carbon and the absence of the diamond peak at 1333 cm−1 (Figure 5b, Supporting Information File 1, Figure S5c-e). The probing depth of Raman scattering is estimated to be about 90 nm (Supporting Information File 1, Table S1). This

• D and 2D bands. The D band represents the disordered vibration modes of graphitic hexagonal layers, and the 2D band originates from the second-order double-resonant scattering process. In general, the quality of graphene layers can be evaluated by the ratio of the intensities of the D and G peaks

Ar⁺ implantation-induced tailoring of RF-sputtered ZnO films: structural, morphological, and optical properties

• Manu Bura,
• Divya Gupta,
• Arun Kumar and
• Sanjeev Aggarwal

Beilstein J. Nanotechnol. 2025, 16, 872–886, doi:10.3762/bjnano.16.66

• disorder-induced band can be ascribed to an increase in dpa, which leads to lattice disorder [14]. The peak corresponding to the A1 (LO) mode of implanted samples is deconvoluted as shown in Figure 6. This type of scattering from the K–M point of the Brillouin zone is symmetrically forbidden. Gupta et al

• reflectance by the following relation [41]: Here R is the diffuse reflectance of the samples; s and α correspond to scattering and absorption coefficients, respectively. The scattering coefficient does not depend on the wavelength. Thus, F(R) becomes proportional to α. It has been observed that with the rise

• . Correlations This anomalous behavior of Raman modes can be attributed to the fact that the incorporation of lattice defects and disorder by energetic ions leads to translational symmetry loss. This results in the breaking of the wave vector k = 0 selection rule required for Raman scattering from different

Insights into the electronic and atomic structures of cerium oxide-based ultrathin films and nanostructures using high-brilliance light sources

• Paola Luches and
• Federico Boscherini

Beilstein J. Nanotechnol. 2025, 16, 860–871, doi:10.3762/bjnano.16.65

• shown as dashed lines. Reprinted from [49] with permission of the Royal Society of Chemistry (“Electronic properties of epitaxial cerium oxide films during controlled reduction and oxidation studied by resonant inelastic X-ray scattering” by G. Gasperi et al., Phys. Chem. Chem. Phys. vol. 18, issue 30