Graphene oxide–chloroquine conjugate induces DNA damage in A549 lung cancer cells through autophagy modulation

• Braham Dutt Arya,
• Sandeep Mittal,
• Prachi Joshi,
• Alok Kumar Pandey,
• Jaime E. Ramirez-Vick,
• Govind Gupta and
• Surinder P. Singh

Beilstein J. Nanotechnol. 2025, 16, 316–332, doi:10.3762/bjnano.16.24

• were obtained from American Type Cell Culture (Cat. No. CCL-185; ATCC, Manassas, VA, USA). Primary antibodies anti-β-actin (Cat. No. 4970), anti-LC-3-I/II (Cat. No. 12741), anti-ATG-7 (Cat. No. 88577) and anti-beclin-1 (Cat. No. 3495) were obtained from Cell Signaling Technology (Danvers, MA, USA). The

• GFP-LC3 plasmid was a kind gift from Dr. Soumya Sinha Roy, CSIR-IGIB, India. The antifade mounting media Vectashield was purchased from Vector Laboratories (Burlingame, CA, USA). All other chemicals were locally obtained and were of analytical reagent grade. Cell culture plastic wares were obtained

• -resolution transmission electron microscope (Technai G2 F30 STWIN, Japan), field emission scanning electron microscope (FEI, Quanta FEG 450, USA), and atomic force microscope (Nanoscope, Veeco V, USA) [28][29][30]. Cell culture A549 cells from the American Type Culture Collection (ATCC, Manassas, VA, USA

Radiosensitizing properties of dual-functionalized carbon nanostructures loaded with temozolomide

• Radmila Milenkovska,
• Nikola Geskovski,
• Dushko Shalabalija,
• Ljubica Mihailova,
• Petre Makreski,
• Dushko Lukarski,
• Igor Stojkovski,
• Maja Simonoska Crcarevska and
• Kristina Mladenovska

Beilstein J. Nanotechnol. 2025, 16, 229–251, doi:10.3762/bjnano.16.18

A nanocarrier containing carboxylic and histamine groups with dual action: acetylcholine hydrolysis and antidote atropine delivery

• Elina E. Mansurova,
• Andrey A. Maslennikov,
• Anna P. Lyubina,
• Alexandra D. Voloshina,
• Irek R. Nizameev,
• Marsil K. Kadirov,
• Anzhela A. Mikhailova,
• Polina V. Mikshina,
• Albina Y. Ziganshina and
• Igor S. Antipin

Beilstein J. Nanotechnol. 2025, 16, 11–24, doi:10.3762/bjnano.16.2

• Cytell Cell Imaging multifunctional system (GE Healthcare Life Science, Sweden) [38]. The experiments utilized a Chang liver cell line (human liver cells) from the N.F. Gamaleya National Research Center for Epidemiology and Microbiology and a cell culture of WI-38 VA 13 subline 2RA (human embryo lung

• ) from the collection of the Institute of Cytology RAS (St. Petersburg, Russia). Standard culture medium Igla, produced by the Moscow Institute of Poliomyelitis and Viral Encephalitis, was employed for cell culture, supplemented with 1% nonessential amino acids (NEAA) and 10% fetal bovine serum. The

Polymer lipid hybrid nanoparticles for phytochemical delivery: challenges, progress, and future prospects

• Iqra Rahat,
• Pooja Yadav,
• Aditi Singhal,
• Mohammad Fareed,
• Jaganathan Raja Purushothaman,
• Mohammed Aslam,
• Raju Balaji,
• Sonali Patil-Shinde and
• Md. Rizwanullah

Beilstein J. Nanotechnol. 2024, 15, 1473–1497, doi:10.3762/bjnano.15.118

• testosterone-induced alopecia suggested that QCT-PLHNPs exhibited significantly higher regrowth of hairs than free QCT. Yin and co-workers developed QCT-encapsulated cholate-modified PLHNPs (QCT-cPLHNPs) to achieve better therapeutic efficacy against leukemia [92]. Cell culture studies suggested that the QCT

• environments. Because of the excellent pharmaceutical attributes and mucoadhesive characteristics, the THQ-PLHNPs show controlled release characteristics and manyfold enhanced intestinal permeation compared to free THQ suspension. Cell culture experiments suggested that the fabricated THQ-PLHNPs significantly

• (TNBC) [120]. IQN-PLHNPs exhibited high stability in the harsh GI milieu. Cell culture experiment suggested that the IQN-PLHNPs showed a nearly twofold increase in cell uptake in MDA-MB-231 cells thereby better cytotoxicity was achieved. IQN-PLHNPs are effectively absorbed from the GI tract and showed a

Dual-functionalized architecture enables stable and tumor cell-specific SiO₂NPs in complex biological fluids

• Iris Renata Sousa Ribeiro,
• Raquel Frenedoso da Silva,
• Romênia Ramos Domingues,
• Adriana Franco Paes Leme and
• Mateus Borba Cardoso

Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100

• improved colloidal stability [25][26]. Remarkably, functionalized NPs were stable in a complex medium (cell culture medium and human plasma) and showed greater potential for recognition by tumor cells. Material and Methods Materials Tetraethyl orthosilicate (TEOS, 98%), (3-aminopropyl)triethoxysilane

• 1.0 mg·mL–1) and later used for the quantification of captured SiO2NPs-ZW-FO. The calculations were performed using the value obtained at the maximum of the emission band. Stability of SiO2NPs in cell culture medium and human plasma Dynamic light scattering (DLS) measurements were performed to

• corona formation in cell culture medium and human plasma To verify the adsorption of proteins on the functionalized SiO2NPs, sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) experiment was performed. For this, the nanomaterials (in concentrations of 2 to 5 mg·mL–1) were incubated in

Synthesis, characterization and anticancer effect of doxorubicin-loaded dual stimuli-responsive smart nanopolymers

• Ömür Acet,
• Pavel Kirsanov,
• Burcu Önal Acet,
• Inessa Halets-Bui,
• Dzmitry Shcharbin,
• Şeyda Ceylan Cömert and
• Mehmet Odabaşı

Beilstein J. Nanotechnol. 2024, 15, 1189–1196, doi:10.3762/bjnano.15.96

• incubation times was studied. Experimental Materials Doxorubicin and all other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). The water utilized in the experiments was purified by a Barnstead (Dubuque, IA, USA) ROpure LP® reverse osmosis unit. Cell culture Experiments were conducted using

Introducing third-generation periodic table descriptors for nano-qRASTR modeling of zebrafish toxicity of metal oxide nanoparticles

• Supratik Kar and
• Siyun Yang

Beilstein J. Nanotechnol. 2024, 15, 1142–1152, doi:10.3762/bjnano.15.93

• ’ kinetics, migration, and transformation than in vitro cell culture assays [14]. Meanwhile, it is considered an equivalent model for investigating developmental toxicity and genotoxicity because around 85% of its genes are comparable to those found in humans [15]. The potential harm to human health posed by

Interface properties of nanostructured carbon-coated biological implants: an overview

• Mattia Bartoli,
• Francesca Cardano,
• Erik Piatti,
• Stefania Lettieri,
• Andrea Fin and
• Alberto Tagliaferro

Beilstein J. Nanotechnol. 2024, 15, 1041–1053, doi:10.3762/bjnano.15.85

• proliferation. The authors reported a significant improvement of cytocompatibility with the creation of a preosteoblastic monolayer on the coated surface after one week of cell culture. Chen and co-workers [95] investigated the effect of GO coating as antifibrotic on metal implants. They controlled the

Therapeutic effect of F127-folate@PLGA/CHL/IR780 nanoparticles on folate receptor-expressing cancer cells

• Thi Ngoc Han Pham,
• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan Thang Cao,
• Thanh-Danh Nguyen,
• Vy Tran Anh and
• Hieu Vu_Quang

Beilstein J. Nanotechnol. 2024, 15, 954–964, doi:10.3762/bjnano.15.78

• pH 5.4. After that, the nanoparticles were centrifuged at 12,000 rpm, and the pellets were collected and freeze-dried. The drugs that remained in the nanoparticle were determined as described above. Cell culture The human breast carcinoma MCF7 cell line, the human hepatoma HepG2 cell line, and the

• form with a core size of around 100 nm (Figure 1B). For this reason, the nanoprecipitation–diffusion technique was utilized to produce the particles employed in the study. Furthermore, after dispersing the nanoparticles in cell culture medium with 10% FBS, the size and PDI of the nanoparticles

• increased, respectively, to 372 nm and 0.486 for F127-folate@PLGA/CHL/IR780, and, respectively, to 288 nm and 0.663 for F127@PLGA/CHL/IR780. The increase in nanoparticle size could be the result of protein absorption the nanoparticle surface [38]. However, the absorption of protein from the cell culture

Electrospun polysuccinimide scaffolds containing different salts as potential wound dressing material

• Veronika Pálos,
• Krisztina S. Nagy,
• Rita Pázmány,
• Krisztina Juriga-Tóth,
• Bálint Budavári,
• Judit Domokos,
• Dóra Szabó,
• Ákos Zsembery and
• Angela Jedlovszky-Hajdu

Beilstein J. Nanotechnol. 2024, 15, 781–796, doi:10.3762/bjnano.15.65

• incubated in cell culture medium for 24 h (37 °C and 5% CO2). The mass of the discs varied, so the applied volume of culture medium was proportionally adjusted (0.1 mg of scaffold in 1 mL of medium). The control medium containing the same salt concentration as the scaffolds were sterile filtered by 0.2 µm

Radiofrequency enhances drug release from responsive nanoflowers for hepatocellular carcinoma therapy

• Yanyan Wen,
• Ningning Song,
• Yueyou Peng,
• Weiwei Wu,
• Qixiong Lin,
• Minjie Cui,
• Rongrong Li,
• Qiufeng Yu,
• Sixue Wu,
• Yongkang Liang,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2024, 15, 569–579, doi:10.3762/bjnano.15.49

• (Solarbio Life Sciences, Beijing, China) at 37 °C for 30 min. Cytotoxicity assays The Huh-7 cells were seeded onto 4-chamber cell culture slides (Nalge Nunc International, Rochester, NY, USA) at a density of 1.0 × 106 cells/chamber. The cells were incubated at 37 °C in 5% CO2 for 24 h. The DMEM medium was

• replaced with fresh medium containing various concentrations of CUR-Fe@MnO2 NFs (10, 25, 50, and 100 µg/mL), and the 4-chamber cell culture slides were placed in a 37 °C water bath. Then, a 0.035 inch heating guidewire (HG) was attached to the bottom of the first of the 4-chamber cell culture slides and

• temperature of each chamber was recorded by a 0.9 mm optical fiber temperature probe (FL-2000, Anritsu Meter Co., Ltd., Tokyo, Japan). The 2.0 × 105 and 1.0 × 106 Huh-7 cells were seeded onto 96-well plates or 4-chamber cell culture slides, and incubated at 37 °C in 5% CO2 for 24 h. According to the above

Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent

• Horacio Emanuel Jerez,
• Yamila Roxana Simioni,
• Kajal Ghosal,
• Maria Jose Morilla and
• Eder Lilia Romero

Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46

• Figure 13. Briefly, HUVECs were seeded in EGM-2 medium at a density of 4 × 104/cm2 on ThinCert™ cell culture inserts (12 wells, 0.4 µm pore size PET membrane Greiner Bio-One GmbH, Austria) previously covered with 2% bovine skin gelatin type B and grown for 24 h. Also, 10.5 × 104/cm2 THP-1 macrophages per

Exploring the relationships between physiochemical properties of nanoparticles and cell damage to combat cancer growth using simple periodic table-based descriptors

• Joyita Roy and
• Kunal Roy

Beilstein J. Nanotechnol. 2024, 15, 297–309, doi:10.3762/bjnano.15.27

• was obtained from Cao et al. [26], where the zeta potential of MeOx NPs was measured in a cell culture of 20% fetal bovine complete medium. Dataset II was taken from Toropova et al. [27], where cell damage measurement was performed based on the uptake of propidium iodide (PI). The dataset is related

Ion beam processing of DNA origami nanostructures

• Leo Sala,
• Agnes Zerolová,
• Violaine Vizcaino,
• Alain Mery,
• Alicja Domaracka,
• Hermann Rothard,
• Philippe Boduch,
• Dominik Pinkas and
• Jaroslav Kocišek

Beilstein J. Nanotechnol. 2024, 15, 207–214, doi:10.3762/bjnano.15.20

• 1013 ions·cm−2. Control samples were adhered at the back of the aluminum plate, in a region not exposed to the ion beam. For the high-energy irradiation, a 60 MeV/u 56Fe25+ beam was used. Samples were irradiated in air on the outer surface of a cell culture flask oriented to face the beam. Maximum flux

Nanocarrier systems loaded with IR780, iron oxide nanoparticles and chlorambucil for cancer theragnostics

• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan-Thang Cao,
• Vy Tran-Anh and
• Hieu Vu Quang

Beilstein J. Nanotechnol. 2024, 15, 180–189, doi:10.3762/bjnano.15.17

• were performed in both 0.1× PBS (13.7 mM of NaCl, 0.27 mM of KCl, 1 mM of Na2HPO4, and 0.18 mM of KH2PO4, pH 7.4) and animal cell culture media containing DMEM and 10% FBS. Scanning electron microscopy For scanning electron microscopy (SEM) experiments, 10 μL of F127-folate@NP was loaded on the silica

• additional 10 min. Cell culture The cell lines HepG2, MCF-7, 3T3, and Hek were cultured in DMEM, 10% FBS, and 1% antibiotic at 37 °C and 5% CO2. Cell uptake estimation The cells were seeded onto 6-well plates at 100,000 cells/well and were cultured overnight. On the next day, the cells were incubated with

• 0.5 mg of F127-folate@NP/Cou-6, F127@NP/Cou-6, and PVA@NP/Cou-6 in 1 mL of cell culture media for 4 h. The cells were then washed with PBS three times and trypsinized. The harvested cells were counted and 20,000 cells/well were added to a black 96-well plate in triplicates. The culture medium and

Berberine-loaded polylactic acid nanofiber scaffold as a drug delivery system: The relationship between chemical characteristics, drug-release behavior, and antibacterial efficiency

• Le Thi Le,
• Hue Thi Nguyen,
• Liem Thanh Nguyen,
• Huy Quang Tran and
• Thuy Thi Thu Nguyen

Beilstein J. Nanotechnol. 2024, 15, 71–82, doi:10.3762/bjnano.15.7

• scaffolds The cell culture medium was prepared by mixing 150 mL of Dulbecco’s modified eagle medium (DMEM) with 600 μL of 0.5 mg/mL trypsin in a Schott bottle. MA-104 cells were distributed into the wells of a 96-well plate in DMEM supplemented with 10% fetal bovine serum. The BBR NPs/PLA nanofiber scaffold

• was cut into circles with a diameter of 6 mm and sterilized with ultraviolet light for 12 h. Then these cut scaffolds were submerged in the cell culture medium in the 96-well plate and incubated for 120 h at 37 °C in an atmosphere of 5% CO2. The morphology of the cultured cells was monitored with an

Curcumin-loaded albumin submicron particles with potential as a cancer therapy: an in vitro study

• Nittiya Suwannasom,
• Netsai Sriaksorn,
• Chutamas Thepmalee,
• Krissana Khoothiam,
• Ausanai Prapan,
• Hans Bäumler and
• Chonthida Thephinlap

Beilstein J. Nanotechnol. 2023, 14, 1127–1140, doi:10.3762/bjnano.14.93

• release for three separate samples was plotted versus time. Ethanol was used in the release medium to enhance solubility and provide sink conditions for poorly water-soluble CUR. Interaction of the microparticles with cells Cell culture The effects of CUR-HSA-MPs on cell cytotoxicity were studied on human

• hepatocellular carcinoma (Huh-7) and human breast adenocarcinoma (MCF-7) cell lines using the colorimetric MTT assay. Human dermal fibroblasts (HDFB) and human cholangiocyte (MMNK-1) cell lines were evaluated as controls for cytotoxicity. Briefly, the cell lines were cultured in cell culture medium containing

• FBS (10%) and penicillin/streptomycin (1%) at 37 °C in a CO2 incubator (95% relative humidity, 5% CO2). Huh-7, HDFB, and MMNK-1 cells were cultured in DMEM, and MCF-7 cells were cultured in RPMI cell culture medium. The cells were then seeded to grow 24 h prior to treatment at a density of 5 × 103

Green SPIONs as a novel highly selective treatment for leishmaniasis: an in vitro study against Leishmania amazonensis intracellular amastigotes

• Brunno R. F. Verçoza,
• Robson R. Bernardo,
• Luiz Augusto S. de Oliveira and
• Juliany C. F. Rodrigues

Beilstein J. Nanotechnol. 2023, 14, 893–903, doi:10.3762/bjnano.14.73

• maintenance of Leishmania amazonensis species in Balb/C mice, the protocol number was UFRJ/CCS-142/21. Furthermore, all animals received human care according to the guide published by the Brazilian Society of Zootechnics of Laboratory and Council National Control of Animal Experimentation. Cell culture The

Nanostructured lipid carriers containing benznidazole: physicochemical, biopharmaceutical and cellular in vitro studies

• Giuliana Muraca,
• María Esperanza Ruiz,
• Rocío C. Gambaro,
• Sebastián Scioli-Montoto,
• María Laura Sbaraglini,
• Gisel Padula,
• José Sebastián Cisneros,
• Cecilia Yamil Chain,
• Vera A. Álvarez,
• Cristián Huck-Iriart,
• Guillermo R. Castro,
• María Belén Piñero,
• Matias Ildebrando Marchetto,
• Catalina Alba Soto,
• Germán A. Islan and
• Alan Talevi

Beilstein J. Nanotechnol. 2023, 14, 804–818, doi:10.3762/bjnano.14.66

• incubation with the formulation of nanoparticles or the free drug. Hemolytic effect Hemolysis was assessed on 3 mL of a freshly drawn heparinized suspension of fresh human blood placed on a 6-well cell culture plate. Increasing concentrations of freshly prepared dilutions of the free drug and NLC-BNZ (1, 5

Quercetin- and caffeic acid-functionalized chitosan-capped colloidal silver nanoparticles: one-pot synthesis, characterization, and anticancer and antibacterial activities

• Akif Hakan Kurt,
• Elif Berna Olutas,
• Fatma Avcioglu,
• Hamza Karakuş,
• Mehmet Ali Sungur,
• Cansu Kara Oztabag and
• Muhammet Yıldırım

Beilstein J. Nanotechnol. 2023, 14, 362–376, doi:10.3762/bjnano.14.31

• sample was recorded at 762 nm. During the measurements, distilled water was used as a reference. Cell culture Human brain glioma (U-118 MG) and human retinal pigment epithelium (ARPE-19) cell lines were purchased from ATCC (NY, USA) and cultured in 10% (v/v) FBS (Gibco; Thermo Scientific, USA) and 1% (v

• comparable to that in the healthy ARPE-19 cells, suggesting that the effect may not be tissue specific. Another issue that may affect the results of biological applications of NPs in general and should be considered is how the colloidal distribution and stability of the NPs are affected in the cell culture

• in the microscope images of Ch/Q- and Ch/CA-Ag NPs in cell medium (see Supporting Information File 1, Figure S1), the stability of the synthesized NPs and how the size changes in cell culture medium compared to water were also evaluated by UV–vis and zeta potential measurements. An increase in the

The steep road to nonviral nanomedicines: Frequent challenges and culprits in designing nanoparticles for gene therapy

• Yao Yao,
• Yeongun Ko,
• Grant Grasman,
• Jeffery E. Raymond and
• Joerg Lahann

Beilstein J. Nanotechnol. 2023, 14, 351–361, doi:10.3762/bjnano.14.30

• be reached, the identification of structure–function and material–biological relations of NPs could be dramatically accelerated. Prevalence of reporting imaging and (or) flow cytometry techniques, protein corona, 3D cell culture model(s), and serum content during nanoparticle incubation with cells in

• investigating NP cellular uptake and (or) transfection. (c) 5-year prevalence of describing or characterizing protein corona in the manuscript. (d) 5-year prevalence of employing 3D cell culture models (e.g., spheroids or organoids). (e) Comparisons of serum content used during nanoparticle incubation with

Nanotechnology – a robust tool for fighting the challenges of drug resistance in non-small cell lung cancer

• Filip Gorachinov,
• Fatima Mraiche,
• Diala Alhaj Moustafa,
• Ola Hishari,
• Yomna Ismail,
• Jensa Joseph,
• Maja Simonoska Crcarevska,
• Marija Glavas Dodov,
• Nikola Geskovski and
• Katerina Goracinova

Beilstein J. Nanotechnol. 2023, 14, 240–261, doi:10.3762/bjnano.14.23

• toxicological profiles [134]. A systematic approach in synthesizing statistical copolymer libraries, fine-tuning nanoparticle biointeractions, and polymer bioresponsiveness, hand in hand with cell culture experiments for fast screening and dynamic cell culture models, may greatly improve the successful outcomes

In search of cytotoxic selectivity on cancer cells with biogenically synthesized Ag/AgCl nanoparticles

• Mitzi J. Ramírez-Hernández,
• Mario Valera-Zaragoza,
• Omar Viñas-Bravo,
• Ariana A. Huerta-Heredia,
• Miguel A. Peña-Rico,
• Erick A. Juarez-Arellano,
• David Paniagua-Vega,
• Eduardo Ramírez-Vargas and
• Saúl Sánchez-Valdes

Beilstein J. Nanotechnol. 2022, 13, 1505–1519, doi:10.3762/bjnano.13.124

• thermal analyzer, with a heating rate of 20 °C/min, under a nitrogen atmosphere, and a temperature range of 30–800 °C. To carry out XRD, EDX, FTIR, and TGA analyses, the pineapple peel extract and the reaction products were previously dried at 110 °C for 24 h. Cell culture In a similar manner to [51], the

Hydroxyapatite–bioglass nanocomposites: Structural, mechanical, and biological aspects

• Olga Shikimaka,
• Mihaela Bivol,
• Bogdan A. Sava,
• Marius Dumitru,
• Christu Tardei,
• Beatrice G. Sbarcea,
• Daria Grabco,
• Constantin Pyrtsac,
• Daria Topal,
• Andrian Prisacaru,
• Vitalie Cobzac and
• Viorel Nacu

Beilstein J. Nanotechnol. 2022, 13, 1490–1504, doi:10.3762/bjnano.13.123

• at −80 °C in FBS (Lonza, Belgium) with 10% DMSO (Alchimia, Italy). The ethical approval, as well as the protocol used for the isolation of the mesenchymal stem cells is described in the Annex. After thawing, cells from the third passage were cultured in DMEM/Ham F12 (Sigma, Germany) cell culture

• , USA) working solution was prepared from 0.5% (w/v) MTT stock solution in DMEM/Ham F12 cell culture media. After removing the samples and cell culture media, MTT working solution was poured in the plates followed by incubation of the plates at 37 °C, 5% CO2 for 2 h, then the formazan crystals were

• (HiMedia, India) and pipetted several times to generate a single-cell suspension. After repeated centrifugation, the cells were resuspended in mesenchymal stem cell expansion medium and transferred into a 25 cm2 cell culture flask and incubated at 37 °C in 5%CO2 humid environment. In the first passage

Facile preparation of Au- and BODIPY-grafted lipid nanoparticles for synergized photothermal therapy

• Yuran Wang,
• Xudong Li,
• Haijun Chen and
• Yu Gao

Beilstein J. Nanotechnol. 2022, 13, 1432–1444, doi:10.3762/bjnano.13.118

• (FOTRIC 225 s, USA). Cell culture Murine breast cancer 4T1 cell line was purchased from Cell Resource Center of Shanghai Institute for Biological Sciences (Chinese Academy of Sciences, Shanghai, China). 4T1 cells were cultured in RPMI-1640 medium containing 100 μg/mL streptomycin, 100 U/mL penicillin, and