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Search for "cytotoxicity" in Full Text gives 218 result(s) in Beilstein Journal of Nanotechnology. Showing first 200.
From shield to spear: Charge-reversible nanocarriers in overcoming cancer therapy barriers
Beilstein J. Nanotechnol. 2026, 17, 159–175, doi:10.3762/bjnano.17.10
- also induce cytotoxicity and rapid clearance by the mononuclear phagocyte system [18]. Moreover, surface charge influences aggregation behaviour, colloidal stability, and protein corona formation, directly impacting the therapeutic efficacy of nanocarriers. Optimising surface charge is essential for
- groups for protonation-driven charge inversion, ensuring precise intracellular payload unloading [22]. Furthermore, the neutral charge state during systemic circulation helps to reduce cytotoxicity by minimising nonspecific protein adsorption and immune system activation. A study by Yuan et al. showed
- [23]. Neutral PEG or hydroxyl-modified nanoparticles demonstrated significantly lower protein binding, opsonization, and phagocytic uptake compared to their charged counterparts, thereby reducing immune clearance and cytotoxicity [24]. Overall, these findings demonstrate that surface charge modulation
Influence of surface characteristics on the in vitro stability and cell uptake of nanoliposomes for brain delivery
Beilstein J. Nanotechnol. 2026, 17, 139–158, doi:10.3762/bjnano.17.9
Development and in vitro evaluation of liposomes and immunoliposomes containing 5-fluorouracil and R-phycoerythrin as a potential phototheranostic system for colorectal cancer
Beilstein J. Nanotechnol. 2026, 17, 97–121, doi:10.3762/bjnano.17.7
- tumor cells, enhancing the internalization of the system and promoting greater cytotoxic activity compared to conventional therapies [10]. By combining selective cytotoxicity with the potential to label cancer tissue using an imaging probe, EGFR-targeted immunoliposomes represent an integrated approach
- evaluated, and the systems were thoroughly characterized. Finally, in vitro experiments in a CRC cell line were conducted for the evaluation of cytotoxicity, photo toxicity, and uptake. Some studies have sought to demonstrate that dual-drug nanosystems can result in synergistic antitumor effects or reduce
- equipment (Malvern Instruments, UK) at the corresponding incubation times [28]. 2.6 Phototoxicity and cytotoxicity The HCT-116 cell line was cultivated in RPMI 1640M medium, supplemented with 10% Fetal Bovine Serum (FBS), and 1% antibiotic/antimycotic solution at 37 °C with 5% CO2, in accordance with ATCC
Internal 3D temperature mapping in biological systems using ratiometric light-sheet imaging and lipid-coated upconversion nanothermometers
Beilstein J. Nanotechnol. 2025, 16, 2306–2316, doi:10.3762/bjnano.16.159
- biocompatibility, low cytotoxicity, and stable optical and chemical properties. Additionally, given the conditions commonly presented in biological samples, these materials should also remain unaffected by changes in pH, concentration, ionic strength, and viscosity [1]. Traditional thermometers are macroscopic
Improving magnetic properties of Mn- and Zn-doped core–shell iron oxide nanoparticles by tuning their size
Beilstein J. Nanotechnol. 2025, 16, 2285–2295, doi:10.3762/bjnano.16.157
- the magnetic saturation and improve hyperthermia performance. However, the cytotoxicity of Co-based nanoparticles poses a significant challenge for biomedical applications. Studies have demonstrated that Co2+ ions released from these particles can enter the bloodstream and accumulate in organs, where
- and shell materials. While CoFe2O4 shells are known to increase magnetic anisotropy, their cytotoxicity limits their use. In contrast, MnFe2O4 shells provide a non-toxic alternative that enhances the overall magnetic performance while maintaining biocompatibility. The orginality of this study lies in
- material to eliminate the cytotoxicity concerns associated with cobalt. Furthermore, we demonstrate size control through synthesis by varying the concentration of oleic acid, a surfactant that influences particle size and morphology. This approach enables the synthesis of NPs with sizes ranging from 5 to
Optical bio/chemical sensors for vitamin B12 analysis in food and pharmaceuticals: state of the art, challenges, and future outlooks
Beilstein J. Nanotechnol. 2025, 16, 2207–2244, doi:10.3762/bjnano.16.153
Quality by design optimization of microemulsions for topical delivery of Passiflora setacea seed oil
Beilstein J. Nanotechnol. 2025, 16, 2116–2131, doi:10.3762/bjnano.16.146
- cytotoxicity and skin irritation [8]. Furthermore, the optimization of microemulsion formulations may be time-consuming and costly, and their stability is often sensitive to environmental factors (e.g., pH, salinity, and temperature). These challenges highlight the importance of a comprehensive understanding
- . The observed cytotoxicity at higher concentrations is likely due to the increase proportion of surfactants in the formulation. Additionally, under certain experimental conditions, fatty acids may induce lipid peroxidation and trigger pro-inflammatory responses in endothelial cells such as HUVECs
- assessments to include additional cell types, particularly keratinocytes and dermal fibroblasts, which are key players in skin regeneration and re-epithelialization [17]. Although the MTT assay is a widely accepted and convenient method for preliminary cytotoxicity screening, it offers limited insight into
Rapid synthesis of highly monodisperse AgSbS2 nanocrystals: unveiling multifaceted activities in cancer therapy, antibacterial strategies, and antioxidant defense
Beilstein J. Nanotechnol. 2025, 16, 2105–2115, doi:10.3762/bjnano.16.145
- ) planes of the AgSbS2 phase. TEM and SEM techniques were used to comprehensively characterize the NCs. The results showed that spherical NCs were predominantly formed, with an average diameter of approximately 32 ± 10 nm. Cytotoxicity studies demonstrated a significant inhibitory effect of the NCs
- retaining a blue color, while the MBC was determined by subculturing non-turbid wells onto MHA plates and identifying the lowest concentration that yielded no visible bacteria colonies after 24 h. Cytotoxicity assays Cell culture and Alamar Blue assay HT-29 colon cancer, MCF-7 breast cancer, and L929
- infections dominated by this pathogen. However, their relatively weaker activity against E. coli and B. subtilis suggests that optimization may be necessary to broaden their spectrum through surface modification, particle size reduction, or combination with other antibacterial agents. Cytotoxicity of AgSbS2
Toward clinical translation of carbon nanomaterials in anticancer drug delivery: the need for standardisation
Beilstein J. Nanotechnol. 2025, 16, 2092–2104, doi:10.3762/bjnano.16.144
- atomic force microscopy. Surface charge plays a significant role in determining how CNMs interact with biological membranes and intracellular environments. It influences processes such as cellular uptake, cytotoxicity, and inflammatory signalling. For example, BSA-derived negatively charged CDs exhibited
- . Depending on the intended route of delivery and application, this may involve cytotoxicity assays to assess effects on cell viability and metabolism, and hemocompatibility assays to evaluate interactions with blood components such as red blood cells, clotting factors, and platelets. In vitro release studies
Targeting the vector of arboviruses Aedes aegypti with nanoemulsions based on essential oils: a review with focus on larvicidal and repellent properties
Beilstein J. Nanotechnol. 2025, 16, 1894–1913, doi:10.3762/bjnano.16.132
- %). The study also demonstrated controlled release of the active ingredient, with 53.3% released in 8 hours (33.31 mg/mL) and 71.5% after 24 hours. Finally, cytotoxicity tests were conducted, wherein the nanoemulsion maintained high cellular compatibility, with viability greater than 97% in murine
On the road to sustainability – application of metallic nanoparticles obtained by green synthesis in dentistry: a scoping review
Beilstein J. Nanotechnol. 2025, 16, 1851–1862, doi:10.3762/bjnano.16.128
- content. Despite promising results, gaps remain, such as the predominance of in vitro studies (68.7%) and insufficient cytotoxicity assessments (47.8%), underscoring the need for translational research. This review highlights the transformative potential of green-synthesized nanoparticles in dentistry
- significantly reduces the generation of toxic waste, occupational risks, and environmental impacts [13][14][15]. Furthermore, green-synthesized nanoparticles demonstrate enhanced biocompatibility, improved bioavailability, and reduced cytotoxicity, which broadens their applicability in fields such as dental
- , including cements, sealants, and mouthwashes [8][45]. CuNPs and nickel oxide nanoparticles, though less frequent, are also gaining attention for their antimicrobial activity, despite some concerns regarding cytotoxicity [46][47]. The presence AuNPs and titanium dioxide nanoparticles highlights an interest
Phytol-loaded soybean oil nanoemulsion as a promising alternative against Leishmania amazonensis
Beilstein J. Nanotechnol. 2025, 16, 1826–1836, doi:10.3762/bjnano.16.126
- presented in Table 1. 3T3 fibroblast-like cell viability Cell viability in mammalian cells was assessed using 3T3 fibroblast-like cells at 24 and 48 hours (Figure 3). Our results showed that none of the treatments induced significant cytotoxicity in this cell type at 24 hours. However, at 48 hours
- , cytotoxicity was observed in cells treated with free PHYT and blank-NE at a concentration of 200 µg/mL, resulting in 67% and 71% cell viability, respectively. Interestingly, PHYT-NE remained safe at all tested concentrations and time points, with cell viability above 80% even at the highest concentration. In
- on cytotoxicity in cutaneous leishmaniasis highlight the immunological interactions involving multiple cell types beyond macrophages, as well as the role of cytotoxic cells in disease progression and tissue damage, supporting the relevance of studying different cellular models, including fibroblasts
Exploring the potential of polymers: advancements in oral nanocarrier technology
Beilstein J. Nanotechnol. 2025, 16, 1751–1793, doi:10.3762/bjnano.16.122
Advances of aptamers in esophageal cancer diagnosis, treatment and drug delivery
Beilstein J. Nanotechnol. 2025, 16, 1734–1750, doi:10.3762/bjnano.16.121
- , which may be due to the fact that the aptamer was not modified and was easily degraded by nucleases. The cytotoxicity of Te4 to TE-1 and HEEC cells showed no potential as a therapeutic agent for EC alone. In addition, Te4 target has been identified as a membrane protein only by flow cytometry, and the
- experiments showed that about 50% of EPI was released after 25 h. Cytotoxicity studies were carried out in KYSE-70 and EC109 cell lines. PEI–aptamer–EPI had significantly higher tumoricidal effects compared with EPI and Aptamer-EPI groups. Unfortunately, this study was only in vitro; biocompatibility, in vivo
Multifunctional anionic nanoemulsion with linseed oil and lecithin: a preliminary approach for dry eye disease
Beilstein J. Nanotechnol. 2025, 16, 1711–1733, doi:10.3762/bjnano.16.120
- absorbance of the nanoformulation, and Absblank represents the absorbance of the sample without DPPH (sample + ethanol). Cytotoxicity assay L929 fibroblast cells were cultured in RPMI 1640 medium supplemented with 10% (v/v) fetal bovine serum (FBS), 1% (v/v) penicillin–streptomycin, and 1% (v/v) GlutaMAX
- , maintained under standard conditions (5% CO2 at 37 °C). Cell viability was determined using the trypan blue exclusion method. The in vitro cytotoxicity of the sterile nanoformulations was evaluated according to the guidelines of the International Organization for Standardization (ISO 10993-5:2009) [59
- the cytotoxicity data analysis, technical replicates were collapsed using the median. Outliers were identified and excluded based on the interquartile range (IQR) method: values above Q3 + 1.5 × IQR or below Q1 − 1.5 × IQR were removed for each experimental group. Normality and homoscedasticity were
Prospects of nanotechnology and natural products for cancer and immunotherapy
Beilstein J. Nanotechnol. 2025, 16, 1644–1667, doi:10.3762/bjnano.16.116
- cytotoxicity. The nanoparticles showed inhibition of the proliferation of breast cancer cells, with lower cytotoxicity to normal liver cells compared to standard drug and free drug. This is due to the nanoparticles’ enhanced permeability and retention, which improves tumor-specific targeting. Mitochondrial
- and drug carriers, and methanol, ethanol, and acetone as solvents. Both nanoparticle systems exhibited high biocompatibility and low cytotoxicity to normal cells. Additionally, the results demonstrated the technology had a tumor inhibition greater than 70% in mice with a synergistic antitumor effect
- properties [142][143]. The tests showed that the nanoparticles containing MTX and DOX were around 400 times more effective and yielded higher cytotoxicity in CT26, MDA-MB-231, and NAR cancer cells, compared to the free drugs [60]. CN117534780 (2024) uses chitosan–glucan nanopolysaccharide complexes (CGCs
Venom-loaded cationic-functionalized poly(lactic acid) nanoparticles for serum production against Tityus serrulatus scorpion
Beilstein J. Nanotechnol. 2025, 16, 1633–1643, doi:10.3762/bjnano.16.115
- carriers, further investigation is required to assess their safety profile. In vitro cytotoxicity assays on relevant cell lines, particularly immune or epithelial cells and long-term biocompatibility studies, including histopathological analysis following repeated administration, will be essential to
Nanotechnology-based approaches for the removal of microplastics from wastewater: a comprehensive review
Beilstein J. Nanotechnol. 2025, 16, 1607–1632, doi:10.3762/bjnano.16.114
- barrier, while MPs of 20 μm can reach internal organs. Exposure occurs through inhalation, posing risks to adults, while children face dangers from MPs in contaminated drinking water. Once inside the body, MPs can trigger neurotoxicity, cytotoxicity, oxidative stress, immune response, metabolic disruption
- been linked to reproductive, immune, and digestive systems through inhalation, ingestion, and dermal exposure. Polystyrene and polyvinyl chloride have been detected in human implants and are associated with carcinogenic effects. These plastics can induce oxidative stress, cytotoxicity, DNA damage
Enhancing the therapeutical potential of metalloantibiotics using nano-based delivery systems
Beilstein J. Nanotechnol. 2025, 16, 1350–1366, doi:10.3762/bjnano.16.98
- steep decrease in the cytotoxicity of the liposomal formulation compared to free gold(III) metalloantibiotic was observed, resulting in an optimal therapeutic index. The formulation also minimized gold-induced cardiotoxicity and cytochrome inhibition, addressing some of the key limitations of gold-based
- tenfold increase in efficacy against M. smegmatis and M. bovis relative to the free Zn complex. Immunofluorescence analyses confirmed selective uptake of Zn-RIF-Tf-QDs by macrophages and dendritic cells, with minimal internalization by lung epithelial cells and no evidence of cytotoxicity or genotoxicity
- against E. coli, S. aureus, and L. monocytogenes. In addition, improved biofilm disruption and reduced cytotoxicity were also observed. Bismuth complexes Bismuth and its derivatives have been widely employed in biomedical applications [130]. Among their most prominent applications is the treatment of
Acrocomia aculeata oil-loaded nanoemulsion: development, anti-inflammatory properties, and cytotoxicity evaluation
Beilstein J. Nanotechnol. 2025, 16, 1277–1288, doi:10.3762/bjnano.16.93
- : Acrocomia aculeata; cytotoxicity; hemolysis; inflammation; nanoemulsion; Introduction Acrocomia aculeata Jacq is a palm of the Arecaceae family, commonly known as bocaiúva or macaúba. It is widespread in South America and is particularly abundant in Mato Grosso do Sul, located in the Center-West region of
- potent hemolytic effect [50]. As shown in Figure 5A, AANE maintained erythrocyte membrane integrity across all tested concentrations, reinforcing its biocompatibility. Furthermore, cytotoxicity evaluation showed that AANE did not inhibit cell growth, as cellular viability remained at 100% across all
- showed a markedly greater anti-inflammatory effect compared to unformulated bocaiúva oil with an efficacy comparable to that of diclofenac. In addition, this nanoemulsion showed no cytotoxicity or hemolytic activity, indicating a favorable safety profile. These findings underscore the potential of the
Better together: biomimetic nanomedicines for high performance tumor therapy
Beilstein J. Nanotechnol. 2025, 16, 1246–1276, doi:10.3762/bjnano.16.92
Hydrogels and nanogels: effectiveness in dermal applications
Beilstein J. Nanotechnol. 2025, 16, 1216–1233, doi:10.3762/bjnano.16.90
- temperature and polymer concentration [130]. Chitosan-based hydrogels have aroused a lot of interest in the health sciences field due to their biocompatibility, biodegradability, and low cytotoxicity [131]. This makes them attractive as a promising material for topical administration of biomolecules such as
- become attractive due to their high biocompatibility and long circulating time in the blood, as well as low cytotoxicity [73]. They can be prepared by various methods, including phase inversion [73], copolymerization [110][111], and double chemical cross-linking reaction [51]. Cellulose derivatives, such
- pH [213]. This microenvironment of skin tumors has been used to modulate site-specific drug delivery, which leads to an increase in the effectiveness of chemotherapy and also a reduction of the cytotoxicity of antineoplastic drugs [211][212]. The pH-responsive nanogel can be designed with cross
Chitosan nanocomposite containing rotenoids: an alternative bioinsecticidal approach for the management of Aedes aegypti
Beilstein J. Nanotechnol. 2025, 16, 1197–1208, doi:10.3762/bjnano.16.88
- , even when delivered via nanocomposites. The cytotoxicity analysis of natural rotenoids to HSF cells revealed no statistically significant differences in cell viability between the control group and the treatments with either in natura rotenoids or the CS/TPP-β-CD–rot nanocomposite (Figure 7; p > 0.05
- ). Both treatments maintained cell viability at levels comparable to the control, indicating the absence of cytotoxic effects. These findings demonstrate that the encapsulation of rotenoids into the CS/TPP-β-CD system does not induce cytotoxicity, similarly to the free compound, suggesting that the
- % improvement in the larvicidal activity rate of the rotenoids in their original forms. The nanocomposites caused morphological and biochemical alterations to the larvae similarly to those caused by in natura rotenoids, but with greater intensity. Cytotoxicity MTT assays using human skin fibroblasts
Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
Beilstein J. Nanotechnol. 2025, 16, 1068–1081, doi:10.3762/bjnano.16.78
- interaction studies Cell viability assay The concentrations and exposure-time-dependent cell viability data for the cells treated with hBNs, hBNs+US, BaTiO3, and BaTiO3+US are shown in Figure 2. As observed, none of the tested concentrations of the NMs exhibited cytotoxicity on HOb cells over a 48 h period
- with PBS before adding the Alamar Blue reagent to the well [59]. However, no significant cytotoxicity was observed after 48 h, suggesting that cells recovered from the transient mechanical stress and restored their membrane function and viability over time. The use of low-intensity ultrasound (20–50 mW
A calix[4]arene-based supramolecular nanoassembly targeting cancer cells and triggering the release of nitric oxide with green light
Beilstein J. Nanotechnol. 2025, 16, 1003–1013, doi:10.3762/bjnano.16.75
- room temperature for at least 48 h, as evidenced by the unaltered values of the hydrodynamic diameter and the unchanged absorption and emission features over this time window. Cytotoxicity and cell targeting properties of 1 The effects of the nanoassembly of 1 on cell viability were evaluated on
- dermal HuDe cells, whose different transporter expression level was confirmed by Wester blotting assay (Figure S10, Supporting Information File 1). Based on the cytotoxicity results, the cells were treated with a non-toxic amount of compound 1 (0.5 μM) for 1 h at 37 °C (see Experimental section). As
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