Engineered PEG–PCL nanoparticles enable sensitive and selective detection of sodium dodecyl sulfate: a qualitative and quantitative analysis

• Soni Prajapati and
• Ranjana Singh

Beilstein J. Nanotechnol. 2025, 16, 385–396, doi:10.3762/bjnano.16.29

• nanoparticles was placed over the diamond point, and the spectra were recorded in the 3900–550 cm−1 range. Optimization of PEG–PCL nanoparticles The concentration and volume of PEG–PCL NPs were optimized using Bradford reagent to obtain maximum absorbance. PEG–PCL NPs (10 mg/mL) were added to the Bradford

• reagent, and spectrophotometric absorbance was measured in different ratios. Finally, different concentrations of PEG–PCL NPs (10–0.005 mg/mL) were added to the Bradford reagent, and colorimetric change was examined. Qualitative detection of sodium dodecyl sulfate using PEG–PCL nanoparticles The

• –PCL) nanoparticles (10 mg/mL), 500 μL of Bradford reagent, and 100 μL of the respective metal ion solution or surfactant, with a total reaction volume of 1 mL. Visual color changes were observed, and absorbance was measured in the 300–900 nm range using a spectrophotometer (Multiskan G0, Thermo

Development of a mucoadhesive drug delivery system and its interaction with gastric cells

• Ahmet Baki Sahin,
• Serdar Karakurt and
• Deniz Sezlev Bilecen

Beilstein J. Nanotechnol. 2025, 16, 371–384, doi:10.3762/bjnano.16.28

• ) was added to the solution and incubated for another 30 min at room temperature. Finally, the absorbance of the solution was recorded at 550 nm in a UV spectrophotometer. To determine the mucoadhesion of the nanoparticles, EudAlg NPs was resuspended in mucin solution (final concentration 0.5 mg/mL, 2

• mL for each time interval). The suspensions were incubated at 37 °C while shaking at 50 rpm. After 15 and 60 min of incubation, the nanoparticles were centrifuged (10,000g for 10 min), and the absorbance of the supernatants was measured at 550 nm to calculate the amount the free mucin that had not

Tailoring of physical properties of RF-sputtered ZnTe films: role of substrate temperature

• Kafi Devi,
• Usha Rani,
• Arun Kumar,
• Divya Gupta and
• Sanjeev Aggarwal

Beilstein J. Nanotechnol. 2025, 16, 333–348, doi:10.3762/bjnano.16.25

• temperature (R.T.) is of stoichiometric ZnTe. The elemental composition of the films changes with increasing substrate temperature. This variation in the atomic percentage of different elements may be due to the difference in the vapour pressures of Zn and Te. Optical properties Transmittance, absorbance, and

• crystallinity of films with increasing substrate temperature. The enhancement of transmittance in the vis–NIR region with increasing substrate temperature indicates a possible application of these films in optoelectronic devices. The variation in absorbance with wavelength for ZnTe films deposited at different

• substrate temperatures is presented in Figure 5B. It has been found that the films exhibit maximum absorbance in the visible region, which later decreases with the increase in wavelength. This decrease in absorbance with wavelength may be due to interband electronic transitions between the conduction band

Recent advances in photothermal nanomaterials for ophthalmic applications

• Jiayuan Zhuang,
• Linhui Jia,
• Chenghao Li,
• Rui Yang,
• Jiapeng Wang,
• Wen-an Wang,
• Heng Zhou and
• Xiangxia Luo

Beilstein J. Nanotechnol. 2025, 16, 195–215, doi:10.3762/bjnano.16.16

• for clinical ILM staining, is better suited for generating VNBs on the ILM, and the high NIR absorbance of ICG is beneficial in the in vivo environment. Karen Peynshaert’s team [169] demonstrated that ICG can bind to the ILM and generate VNBs upon pulsed laser irradiation, thereby disrupting the

Clays enhanced with niobium: potential in wastewater treatment and reuse as pigment with antibacterial activity

• Silvia Jaerger,
• Patricia Appelt,
• Mario Antônio Alves da Cunha,
• Fabián Ccahuana Ayma,
• Ricardo Schneider,
• Carla Bittencourt and
• Fauze Jacó Anaissi

Beilstein J. Nanotechnol. 2025, 16, 141–154, doi:10.3762/bjnano.16.13

• spectrometer using KBr pellets containing 1% by weight of the samples. Analyses of all samples were performed in the range of 4000 to 400 cm−1 with a resolution of 4 cm−1, accumulating 10 scans. Absorbance measurements of the supernatant solutions were analyzed using a UV–vis spectrophotometer (UV-1800

• SHIMADZU) with a 1 cm path length glass cuvette at λmax (maximum absorbance) in nanometers. Samples collected after the adsorption process had the electronic spectra analyzed using an Ocean Optics USB-2000 instrument for solid samples with a tungsten lamp in the range of 200–800 nm in diffuse reflectance

• the average particle size (D50) for them was 4.52 and 86 μm, respectively. These values indicate that the clay aggregates are dissociated and well dispersed, as shown in the study by Yang et al. [20]. The absorbance spectra profiles in the visible region for the bentonite samples and bentonite

Characterization of ZnO nanoparticles synthesized using probiotic Lactiplantibacillus plantarum GP258

• Prashantkumar Siddappa Chakra,
• Aishwarya Banakar,
• Shriram Narayan Puranik,
• Vishwas Kaveeshwar,
• C. R. Ravikumar and
• Devaraja Gayathri

Beilstein J. Nanotechnol. 2025, 16, 78–89, doi:10.3762/bjnano.16.8

• photocatalyst and 20 ppm of MB dye was exposed to UV light in order to initiate photodegradation. Measurements were taken at 15 min intervals to track the reduction in MB dye concentration. UV–visible spectrophotometry was used to measure the absorbance at 663 nm. Under UV irradiation, the photodegradation of

• this, 20 μL of freshly prepared MTT solution was introduced to each well, and the resulting absorbance was quantified at 570 nm. This experimental procedure was conducted in triplicates [22][33]. Phylogenetic relationship of GP258 and its evolutionary positioning within the Lactiplantibacillus genus

A nanocarrier containing carboxylic and histamine groups with dual action: acetylcholine hydrolysis and antidote atropine delivery

• Elina E. Mansurova,
• Andrey A. Maslennikov,
• Anna P. Lyubina,
• Alexandra D. Voloshina,
• Irek R. Nizameev,
• Marsil K. Kadirov,
• Anzhela A. Mikhailova,
• Polina V. Mikshina,
• Albina Y. Ziganshina and
• Igor S. Antipin

Beilstein J. Nanotechnol. 2025, 16, 11–24, doi:10.3762/bjnano.16.2

• in PBS to reach a final volume of 5 mL (final erythrocyte concentration, 10% v/v). The resultant suspension was agitated and incubated at 37 °C for 1 h. After that, the samples were centrifuged for 10 min at 2000 rpm. The absorbance of the supernatant at 540 nm was used to calculate the amount of

Mechanistic insights into endosomal escape by sodium oleate-modified liposomes

• Ebrahim Sadaqa,
• Satrialdi,
• Fransiska Kurniawan and
• Diky Mudhakir

Beilstein J. Nanotechnol. 2024, 15, 1667–1685, doi:10.3762/bjnano.15.131

• ). The plates were incubated for an additional 3 h at 37 °C to facilitate the reduction of resazurin by the cells. Absorbance was subsequently measured at 570 and 600 nm using a microplate reader, providing data on cell viability. Cellular uptake assay 4T1 triple-negative breast cancer cells were seeded

Facile synthesis of size-tunable L-carnosine-capped silver nanoparticles and their role in metal ion sensing and catalytic degradation of p-nitrophenol

• Akash Kumar,
• Ridhima Chadha,
• Abhishek Das,
• Nandita Maiti and
• Rayavarapu Raja Gopal

Beilstein J. Nanotechnol. 2024, 15, 1576–1592, doi:10.3762/bjnano.15.124

• sustainability and public health. Silver nanoparticles (AgNPs) have attracted the interest of researchers worldwide in recent years because of their promising use in environmental chemistry. The unique optochemical properties of AgNPs, including high surface area to volume ratio, optical absorbance, excellent

• was air-dried. Characterization of ʟ-carnosine-capped silver nanoparticles The spectral absorbance of ʟ-car-AgNPs was recorded using an Epoch2 spectrophotometer (BioTek, USA) in the 300–900 nm range. Zeta potentials and average hydrodynamic diameters of ʟ-car-AgNPs were determined using a Zetasizer

• degradation of P-NP and the formation of P-AP were monitored by recording UV–vis spectra in the 300–900 nm range. The disappearance of the yellow color, characteristic of the nitrophenolate ion, was observed upon the addition of the AgNP catalyst, accompanied by a concomitant decrease in the absorbance

Ultrablack color in velvet ant cuticle

• Vinicius Marques Lopez,
• Wencke Krings,
• Juliana Reis Machado,
• Stanislav Gorb and
• Rhainer Guillermo-Ferreira

Beilstein J. Nanotechnol. 2024, 15, 1554–1565, doi:10.3762/bjnano.15.122

• : Salticidae), and the bird-of-paradise Drepanornis bruijnii (Passeriformes: Paradisaeidae). The spectra of these species were obtained from data provided in [10][12], and species with the highest absorbance in the UV–vis spectrum were selected. Thermal images This experimental protocol was adapted from [15

• ultrastructure consisting of randomly oriented anisotropic micropillars accounts for its significant absorptivity [27]. Peacock spiders feature microstructures in specific body regions that exhibit ultrablack coloration with high absorbance. Interestingly, these microstructures are absent in other dark body

• areas with lower UV–vis absorbance [10]. These microstructures scatter light across the absorptive cuticle, further aided by brush-like scales that serve as an anti-reflective coating [10]. Similarly, birds-of-paradise display ultrablack coloration attributed to microstructures found in the barbules of

A biomimetic approach towards a universal slippery liquid infused surface coating

• Ryan A. Faase,
• Madeleine H. Hummel,
• AnneMarie V. Hasbrook,
• Andrew P. Carpenter and
• Joe E. Baio

Beilstein J. Nanotechnol. 2024, 15, 1376–1389, doi:10.3762/bjnano.15.111

• was added to each well of a 96-well plate blocked with BSA. After incubation for 60 min, 90 μL of protein solution was removed and added to a new plate containing 5 μL 4.7mM soybean trypsin inhibitor (Sigma). 5 μL of chromogenic substrate S-2302 (Chromogenix) was added to each well and the absorbance

• 7.4, 150 mM NaCl). Plasma was then recalcified with 50 μL 25 mM CaCl2. Absorbance measurements were taken every 1 min for 60 min at 405 nm. Three separate experiments were performed for a total sample size of 18 per donor. Fibrin generation time was defined as the time to reach a 5% increase over the

• baseline absorbance value. 5% is a sufficient threshold to overcome the noise floor of the data [33]. Clot morphology Wells of interest of a non-treated 96-well polystyrene were blocked with 300 μL 1% BSA in Milli-Q water for 2 h at 37 °C. After 2 h, wells were rinsed three times with Milli-Q water. Plates

Nanoarchitectonics with cetrimonium bromide on metal nanoparticles for linker-free detection of toxic metal ions and catalytic degradation of 4-nitrophenol

• Akash Kumar and
• Raja Gopal Rayavarapu

Beilstein J. Nanotechnol. 2024, 15, 1312–1332, doi:10.3762/bjnano.15.106

• change in surface plasmon resonance of the metal nanostructures. A single absorbance peak correlates to the symmetrical shape and collective oscillation of free electrons on the nanoparticle surface. This phenomenon is known as localized surface plasmon resonance (LSPR), a characteristic feature of

• concentration [48]. Centrifuged and as-prepared AuNS0.5 and AuNS1.0 showed no absorbance or colorimetric change in the presence of NaCl (Figure 4e,f). Similarly, centrifuged and as-prepared AuNR1s (AuNR10.5 and AuNR11.0) showed no changes in plasmon frequency or color of the nanoparticles in the presence of

• +, each with a concentration of 1 ppm, was examined. The isotropic CTAB-capped AgNS and AuNS were used to detect heavy metals without NaOH. Centrifuged CTAB-AgNS0.5 detected Hg2+ and Fe3+ based on changes in color and spectral absorbance compared to the control (Figure 5a). Surprisingly, no metal

Mn-doped ZnO nanopowders prepared by sol–gel and microwave-assisted sol–gel methods and their photocatalytic properties

• Cristina Maria Vlăduț,
• Crina Anastasescu,
• Silviu Preda,
• Oana Catalina Mocioiu,
• Simona Petrescu,
• Jeanina Pandele-Cusu,
• Dana Culita,
• Veronica Bratan,
• Ioan Balint and
• Maria Zaharescu

Beilstein J. Nanotechnol. 2024, 15, 1283–1296, doi:10.3762/bjnano.15.104

• /min. Characterization Fourier transform infrared spectra (FTIR) were recorded with a Nicolet 6700 apparatus in the range of 400–4000 cm−1. The resolution was 4 cm−1. The powders were pressed in KBr pellets, and the spectra were recorded in absorbance mode. Differential thermal analysis (DTA) and

Dual-functionalized architecture enables stable and tumor cell-specific SiO₂NPs in complex biological fluids

• Iris Renata Sousa Ribeiro,
• Raquel Frenedoso da Silva,
• Romênia Ramos Domingues,
• Adriana Franco Paes Leme and
• Mateus Borba Cardoso

Beilstein J. Nanotechnol. 2024, 15, 1238–1252, doi:10.3762/bjnano.15.100

• absorbance value at 562 nm, using the Thermo microplate reader (Multiskan GO model). For quantification, it was necessary to prepare an analytical curve with diluted BSA standards already included in the kit provided by the manufacturer. Analyses were performed in quintuplicate. To identify the main proteins

• μL of supernatant was removed from each tube and transferred to a 96-well plate. The supernatant hemoglobin quantification was done by measuring the absorbance of the solution (540 nm) with a microplate reader (Thermo, Multiskan GO model). Deionized water (900 μL) and RBC stock suspension (100 μL

• concentration at an absorbance wavelength of 540 nm. The blood was then diluted to a hemoglobin concentration of 10 mg·mL–1 with Ca2+/Mg2+-free PBS. Functionalized and non-functionalized SiO2NPs were evaluated at concentrations of 0.5, 1.0, 3.0, and 5.0 mg·mL–1. Aliquots were prepared for a final volume of 1 mL

Enhanced catalytic reduction through in situ synthesized gold nanoparticles embedded in glucosamine/alginate nanocomposites

• Chi-Hien Dang,
• Le-Kim-Thuy Nguyen,
• Minh-Trong Tran,
• Van-Dung Le,
• Nguyen Minh Ty,
• T. Ngoc Han Pham,
• Hieu Vu-Quang,
• Tran Thi Kim Chi,
• Tran Thi Huong Giang,
• Nguyen Thi Thanh Tu and
• Thanh-Danh Nguyen

Beilstein J. Nanotechnol. 2024, 15, 1227–1237, doi:10.3762/bjnano.15.99

• tracked over time using a UV–vis spectrophotometer, covering the wavelength range of 200–600 nm at room temperature without delay. We examined the kinetics of catalytic degradation by monitoring changes in absorbance values at specific wavelengths. Given the small quantity of nanocatalyst and the

• temperature, and the reaction time, by using UV–vis spectroscopy. Changes in the physicochemical properties, such as morphology and particle size of AuNPs, were monitored through absorbance and the λmax values of the surface plasmon resonance (SPR) band. Figure 2 illustrates the impact of synthesis conditions

• on the formation of AuNPs@GluN/Alg. We explored the Au3+ ion-to-gel ratios ranging from 1% to 9% (w/w). The results indicate that the concentration of Au3+ ions profoundly influences the formation of stable AuNPs. An increase in Au3+ concentration led to a corresponding rise in the absorbance values

Unveiling the potential of alginate-based nanomaterials in sensing technology and smart delivery applications

• Shakhzodjon Uzokboev,
• Khojimukhammad Akhmadbekov,
• Ra’no Nuritdinova,
• Salah M. Tawfik and
• Yong-Ill Lee

Beilstein J. Nanotechnol. 2024, 15, 1077–1104, doi:10.3762/bjnano.15.88

Effect of wavelength and liquid on formation of Ag, Au, Ag/Au nanoparticles via picosecond laser ablation and SERS-based detection of DMMP

• Sree Satya Bharati Moram,
• Chandu Byram and
• Venugopal Rao Soma

Beilstein J. Nanotechnol. 2024, 15, 1054–1069, doi:10.3762/bjnano.15.86

• recorded SERS spectra, after which SERS calculations were undertaken. Results and Discussion Characterization Optical absorption studies of as-synthesized nanoparticles The absorbance measurements were carried out on metallic Ag, Au, and Ag/Au NPs prepared using ps LASiS in both DW and aqueous NaCl

• , and aggregation variations, ultimately impacting their optical properties [53]. Also, from absorbance studies, it is observed that the ablation rate is higher for the Au target than for Ag because of the hardness variation observed in an earlier report by Solati et al. [54]. In another study by Bae et

• al. [55], the authors demonstrated the Ag NP fabrication by varying the surrounding aqueous NaCl solution concentrations between 0 to 20 mM using a nanosecond laser at an excitation of 355 nm. Their study noticed increased Ag NP absorbance while changing the aqueous NaCl solution concentration from 0

Therapeutic effect of F127-folate@PLGA/CHL/IR780 nanoparticles on folate receptor-expressing cancer cells

• Thi Ngoc Han Pham,
• Phuong-Thao Dang-Luong,
• Hong-Phuc Nguyen,
• Loc Le-Tuan,
• Xuan Thang Cao,
• Thanh-Danh Nguyen,
• Vy Tran Anh and
• Hieu Vu_Quang

Beilstein J. Nanotechnol. 2024, 15, 954–964, doi:10.3762/bjnano.15.78

• ]. Consequently, our research employed these two compounds to evaluate the efficacy of our nanoparticle design. The drug loading in nanoparticles was measured by measuring the absorbance at 258 nm for CHL and 780 nm for IR780. The results indicate that the nanoparticles contained 0.5% CHL and 0.26% IR780 (Table 1

Intermixing of MoS₂ and WS₂ photocatalysts toward methylene blue photodegradation

• Maryam Al Qaydi,
• Nitul S. Rajput,
• Michael Lejeune,
• Abdellatif Bouchalkha,
• Mimoun El Marssi,
• Steevy Cordette,
• Chaouki Kasmi and
• Mustapha Jouiad

Beilstein J. Nanotechnol. 2024, 15, 817–829, doi:10.3762/bjnano.15.68

• evaluated the PD of the MB dye solution under light excitation without any photocatalysts (MB photolysis) as well as its PD in the dark in the presence of a photocatalyst. The optical absorbance spectra of the MB solution in the presence of MoS2 and WS2 were recorded in the dark and under visible light

• composite. g–i) High-resolution STEM-EDS mapping of the MoS2/WS2 composite depicting a random presence of W atoms (marked by circles). PD of MB recorded under solar simulator excitation: MB absorbance variation at various durations using a) MoS2 and b) WS2 photocatalysts. The relative MB concentration

Electrospun polysuccinimide scaffolds containing different salts as potential wound dressing material

• Veronika Pálos,
• Krisztina S. Nagy,
• Rita Pázmány,
• Krisztina Juriga-Tóth,
• Bálint Budavári,
• Judit Domokos,
• Dóra Szabó,
• Ákos Zsembery and
• Angela Jedlovszky-Hajdu

Beilstein J. Nanotechnol. 2024, 15, 781–796, doi:10.3762/bjnano.15.65

• was removed from each well and 200 µL of phosphate-buffered saline was added to eliminate the residual media as well as cells that were either loosely attached or did not attach to the plate. Then, 100 µL of diluted WST-1 reagent was added to the cells and incubated for 4 h. The absorbance values of

Green synthesis of biomass-derived carbon quantum dots for photocatalytic degradation of methylene blue

• Dalia Chávez-García,
• Mario Guzman,
• Viridiana Sanchez and
• Rubén D. Cadena-Nava

Beilstein J. Nanotechnol. 2024, 15, 755–766, doi:10.3762/bjnano.15.63

• a dark environment to ensure proper dispersion of the CQDs in aqueous media and the adsorption of the dye on the CQD surface. After the stirring period of 20 min, the solution absorbance was measured using a spectrophotometer in the wavelength range of 250–800 nm. This measurement marked the start

• incandescent light were used, and the solution was continuously agitated. The absorbance of the solution was measured at 20 min intervals to monitor the progress of the degradation reaction. All samples were evaluated under both light sources. Results and Discussion UV–vis spectroscopy UV–vis spectroscopy was

• ). It is observed that for each of the samples, its maximum absorption peak is approximately at 350 nm, which corresponds to n–π* transitions present in unsaturated compounds with heteroatoms (carbonyl groups). An absorbance tail is also noticed in the visible range [31][32]. The samples M6 and M7

Simultaneous electrochemical determination of uric acid and hypoxanthine at a TiO₂/graphene quantum dot-modified electrode

• Vu Ngoc Hoang,
• Dang Thi Ngoc Hoa,
• Nguyen Quang Man,
• Le Vu Truong Son,
• Le Van Thanh Son,
• Vo Thang Nguyen,
• Le Thi Hong Phong,
• Ly Hoang Diem,
• Kieu Chan Ly,
• Ho Sy Thang and
• Dinh Quang Khieu

Beilstein J. Nanotechnol. 2024, 15, 719–732, doi:10.3762/bjnano.15.60

• suspensions. The UV–vis spectra show absorbance bands from 200–400 nm that are typical for the colloidal systems with nanoscale particles [24][25]. The UV–vis spectrum of GQDs shows a characteristic feature of GO at ≈300 nm due to the absorption of the graphitic structure [26], while that of titanium

Gold nanomakura: nanoarchitectonics and their photothermal response in association with carrageenan hydrogels

• Nabojit Das,
• Vikas,
• Akash Kumar,
• Sanjeev Soni and
• Raja Gopal Rayavarapu

Beilstein J. Nanotechnol. 2024, 15, 678–693, doi:10.3762/bjnano.15.56

• of 1 mL of each sample after one wash was taken into consideration for the measurement. The absorbance range was set to 300–900 nm during all measurements. The zeta potential measurement was performed using a Zetasizer (Nano ZS, Malvern UK). The sample was transferred into a DTS1070 cuvette and the

Radiofrequency enhances drug release from responsive nanoflowers for hepatocellular carcinoma therapy

• Yanyan Wen,
• Ningning Song,
• Yueyou Peng,
• Weiwei Wu,
• Qixiong Lin,
• Minjie Cui,
• Rongrong Li,
• Qiufeng Yu,
• Sixue Wu,
• Yongkang Liang,
• Wei Tian and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2024, 15, 569–579, doi:10.3762/bjnano.15.49

• ., Ltd., China). Then, 2 mL of samples was collected from PBS at different time points (0, 4, 24, 48, and 72 h) and replenished with an equal volume of PBS. The absorbance of each of the samples was measured with a microplate reader at a wavelength of 426 nm to determine the concentration of CUR. The Mn2

Cholesterol nanoarchaeosomes for alendronate targeted delivery as an anti-endothelial dysfunction agent

• Horacio Emanuel Jerez,
• Yamila Roxana Simioni,
• Kajal Ghosal,
• Maria Jose Morilla and
• Eder Lilia Romero

Beilstein J. Nanotechnol. 2024, 15, 517–534, doi:10.3762/bjnano.15.46

• incubation, the MTT solution was removed, the insoluble formazan crystals were dissolved in dimethyl sulfoxide (DMSO), and absorbance was measured at 570 nm in a Cytation™ 5 cell imaging multi-mode reader (Biotek Instruments, VT, USA). The cell viability was expressed as a percentage of the cells grown in

• . Finally, the absorbance was measured at 550 nm as stated before. Uptake of nanovesicles The uptake of RhPE-labelled nanovesicles by THP-1 monocytes and macrophages was measured by flow cytometry. Briefly, THP-1 monocytes were seeded on 24-well culture plates at a density of 1 × 106 cells per well (150 µL

• of upper and basolateral compartments were collected and stored at −20 °C for further analysis. Human TNF-α, IL-8, and IL-6 levels were measured by enzyme-linked immunosorbent assay (BD OptEIA™, BD Biosciences, San Jose, CA, USA) following the manufacturer’s guidelines. Absorbance measurements were