Characterization, bio-uptake and toxicity of polymer-coated silver nanoparticles and their interaction with human peripheral blood mononuclear cells

• Sahar Pourhoseini,
• Reilly T. Enos,
• Angela E. Murphy,
• Bo Cai and
• Jamie R. Lead

Beilstein J. Nanotechnol. 2021, 12, 282–294, doi:10.3762/bjnano.12.23

• Healthcare Bio-Sciences AB, Sweden) [65][66]. In brief, peripheral blood from healthy volunteers was diluted with an equal volume of sterile phosphate-buffered saline (PBS; Gibco by life Technologies) and slowly layered onto the Ficoll-Paque media solution. Tubes were centrifuged at 500g for 30 to 40 min at

Differences in surface chemistry of iron oxide nanoparticles result in different routes of internalization

• Barbora Svitkova,
• Vlasta Zavisova,
• Veronika Nemethova,
• Martina Koneracka,
• Miroslava Kretova,
• Filip Razga,
• Monika Ursinyova and
• Alena Gabelova

Beilstein J. Nanotechnol. 2021, 12, 270–281, doi:10.3762/bjnano.12.22

• -Dynamin I/II (#2342) (Cell Signaling Technology, BioTech Ltd., Slovakia), and anti-GAPDH (Sigma-Aldrich, Lambda Life, Ltd., Slovakia). Secondary peroxidase-labeled donkey anti-rabbit IgG (GE Healthcare, Germany) antibodies were visualized with Luminol and coumaric acid (Sigma-Aldrich, Slovakia). Atomic

Toward graphene textiles in wearable eye tracking systems for human–machine interaction

• Ata Jedari Golparvar and
• Murat Kaya Yapici

Beilstein J. Nanotechnol. 2021, 12, 180–189, doi:10.3762/bjnano.12.14

• have many applications ranging from behavioral analytics to healthcare. For a brand leader, the prospect of seeing the world literally through consumer’s eyes, as opposed to relying on traditional market research methods, is the reason that makes eye tracking a clear winner in understanding the

• triggering subconscious facts in decision making. In entertainment and virtual reality applications, eye tracking enables a whole new interaction method with contents, and it could also add new security measures through retinal scanning. In healthcare, eye tracking enables devices to help ease a disabled

• experience on virtual reality gaming [16] or smartphone operation [17]. Along these lines, in the healthcare domain, as part of a hospital alarm system, EOG-based switches provided immobile patients with a safe and reliable way of signaling an alarm [18]. Other promising studies reported the direct input of

Walking energy harvesting and self-powered tracking system based on triboelectric nanogenerators

• Mingliang Yao,
• Guangzhong Xie,
• Qichen Gong and
• Yuanjie Su

Beilstein J. Nanotechnol. 2020, 11, 1590–1595, doi:10.3762/bjnano.11.141

• excellent performance, which can be used as motion [35][36][37] and temperature sensors [38][39], UV detectors [40], tactile sensors [41][42][43], sensors for healthcare [44][45][46][47], humidity sensors, and gas sensors [48][49][50][51], for example. In this work, a flexible undulated electrode-based

Antimicrobial metal-based nanoparticles: a review on their synthesis, types and antimicrobial action

• Matías Guerrero Correa,
• Fernanda B. Martínez,
• Cristian Patiño Vidal,
• Camilo Streitt,
• Juan Escrig and
• Carol Lopez de Dicastillo

Beilstein J. Nanotechnol. 2020, 11, 1450–1469, doi:10.3762/bjnano.11.129

• their excellent properties, CuO NPs have attracted great interest from the healthcare, food packaging, medical, and environmental industries [120][134]. This metal oxide is capable of disrupting the normal function of the cell membrane, changing its permeability and the cellular respiration process [135

Uniform Fe₃O₄/Gd₂O₃-DHCA nanocubes for dual-mode magnetic resonance imaging

• Miao Qin,
• Yueyou Peng,
• Mengjie Xu,
• Hui Yan,
• Yizhu Cheng,
• Xiumei Zhang,
• Di Huang,
• Weiyi Chen and
• Yanfeng Meng

Beilstein J. Nanotechnol. 2020, 11, 1000–1009, doi:10.3762/bjnano.11.84

• until the solution was clear and transparent. After that, the solution was diluted in 10 mL of ultrapure water and finally could be used for testing. In vitro and in vivo magnetic resonance images were captured by a 3.0 T MRI scanner (MAGNETOM Skyra, Siemens Healthcare, Erlangen, Germany). The

Key for crossing the BBB with nanoparticles: the rational design

• Sonia M. Lombardo,
• Marc Schneider,
• Akif E. Türeli and
• Nazende Günday Türeli

Beilstein J. Nanotechnol. 2020, 11, 866–883, doi:10.3762/bjnano.11.72

• nanoparticles (AuNPs); blood–brain barrier (BBB); drug delivery; liposomes; nanomedicine; polymeric nanoparticles; solid lipid nanoparticles; superparamagnetic iron oxide nanoparticles (SPIONs); Introduction Neurological disorders and brain diseases are real burdens for modern societies and healthcare systems

Bombesin receptor-targeted liposomes for enhanced delivery to lung cancer cells

• Mohammad J. Akbar,
• Pâmela C. Lukasewicz Ferreira,
• Melania Giorgetti,
• Leanne Stokes and
• Christopher J. Morris

Beilstein J. Nanotechnol. 2019, 10, 2553–2562, doi:10.3762/bjnano.10.246

• healthcare and diagnostic technologies is limited [2]. Alarmingly, the average life expectancy of an untreated SCLC patient is less than four months due to the high likelihood of diagnosis at the metastatic stage. With intensive chemo- and radiotherapy the median survival extends to only 14–18 months meaning

Enhanced inhibition of influenza virus infection by peptide–noble-metal nanoparticle conjugates

• Zaid K. Alghrair,
• David G. Fernig and
• Bahram Ebrahimi

Beilstein J. Nanotechnol. 2019, 10, 1038–1047, doi:10.3762/bjnano.10.104

• (GE Healthcare Bio-Sciences AB, Sweden). The gel slurry was packed into a white pipette tip (200 µL) using half the filter as a frit and equilibrated in PBS. Capped nanoparticles were concentrated and exchanged into the appropriate buffer using a 10 kDa cut-off Nanosep centrifugal filter. The

Effects of gold and PCL- or PLLA-coated silica nanoparticles on brain endothelial cells and the blood–brain barrier

• Aniela Bittner,
• Angélique D. Ducray,
• Hans Rudolf Widmer,
• Michael H. Stoffel and
• Meike Mevissen

Beilstein J. Nanotechnol. 2019, 10, 941–954, doi:10.3762/bjnano.10.95

• 24 h at 37 °C and 5% CO2. Subsequently, the uptake of PCL- and PLLA-NPs was assessed using a Zeiss Axio Imager Z1 plus Apotome 1 (Carl Zeiss Vision Swiss AG, Feldbach, Switzerland) and evaluated quantitatively by high-content analysis with the IN Cell Analyzer 2000 (GE Healthcare Life Sciences, USA

Comparative biological effects of spherical noble metal nanoparticles (Rh, Pd, Ag, Pt, Au) with 4–8 nm diameter

• Alexander Rostek,
• Marina Breisch,
• Kevin Pappert,
• Kateryna Loza,
• Marc Heggen,
• Manfred Köller,
• Christina Sengstock and
• Matthias Epple

Beilstein J. Nanotechnol. 2018, 9, 2763–2774, doi:10.3762/bjnano.9.258

• biological effects of rhodium nanoparticles except that these nanoparticles can penetrate into human skin [32]. Silver nanoparticles are applied in various fields including healthcare and biomedicine due to their antimicrobial, antifungal and antiviral effect [33][34][35][36][37]. This is based on the

Droplet-based synthesis of homogeneous magnetic iron oxide nanoparticles

• Christian D. Ahrberg,
• Ji Wook Choi and
• Bong Geun Chung

Beilstein J. Nanotechnol. 2018, 9, 2413–2420, doi:10.3762/bjnano.9.226

• healthcare significantly [5]. For example, a composite nanomaterial has been developed as a photosensitizer in photothermal therapy (PTT), while also acting as a contrast agent for magnetic resonance imaging (MRI) [6]. Magnetic materials are of particular interest here, as they can be used for targeting [7

Electrospun one-dimensional nanostructures: a new horizon for gas sensing materials

• Muhammad Imran,
• Nunzio Motta and
• Mahnaz Shafiei

Beilstein J. Nanotechnol. 2018, 9, 2128–2170, doi:10.3762/bjnano.9.202

• applications in numerous areas, including healthcare, energy storage, biotechnology, environmental monitoring, and defence/security. Their enhanced specific surface area, superior mechanical properties, nanoporosity and improved surface characteristics (in particular, uniformity and stability) have made them

• and reversibility, are strongly influenced by the properties of the sensing materials [12][13][14]. Chemical sensors are widely used for biomedical, healthcare, security and environmental applications. Table S1 in Supporting Information File 1 presents a summary of the different types of electrospun

Review on nanoparticles and nanostructured materials: history, sources, toxicity and regulations

• Jaison Jeevanandam,
• Ahmed Barhoum,
• Yen S. Chan,
• Alain Dufresne and
• Michael K. Danquah

Beilstein J. Nanotechnol. 2018, 9, 1050–1074, doi:10.3762/bjnano.9.98

• , 2001 [107]. This indicates that extensive studies should be carried out amongst workers of demolition sites to identify the ill-effect of particles that are dissipated. Nanoparticles in biomedical and healthcare products: NMs are incorporated in cosmetics and sunscreens as antioxidants [108] and

Green synthesis of fluorescent carbon dots from spices for in vitro imaging and tumour cell growth inhibition

• Nagamalai Vasimalai,
• Vânia Vilas-Boas,
• Juan Gallo,
• María de Fátima Cerqueira,
• Mario Menéndez-Miranda,
• José Manuel Costa-Fernández,
• Lorena Diéguez,
• Begoña Espiña and
• María Teresa Fernández-Argüelles

Beilstein J. Nanotechnol. 2018, 9, 530–544, doi:10.3762/bjnano.9.51

• , antibiotic mixture of penicillin/streptomycin (10,000 U·mL−1/10,000 μg·mL−1), fungizone (250 μg·mL−1), human transferrin (4 mg·mL−1) and phosphate buffered saline solution (1× PBS) were obtained from GIBCO Invitrogen (Barcelona, Spain). Fetal bovine serum (FBS) was obtained from HyClone GE Healthcare (United

Hyperthermic intracavitary nanoaerosol therapy (HINAT) as an improved approach for pressurised intraperitoneal aerosol chemotherapy (PIPAC): Technical description, experimental validation and first proof of concept

• Daniel Göhler,
• Stephan Große,
• Alexander Bellendorf,
• Thomas Albert Falkenstein,
• Mehdi Ouaissi,
• Jürgen Zieren,
• Michael Stintz and
• Urs Giger-Pabst

Beilstein J. Nanotechnol. 2017, 8, 2729–2740, doi:10.3762/bjnano.8.272

• tomography (CT) was performed at 130 kV that led to a slice thickness of 5 mm. Three-dimensional images were iteratively reconstructed based on four iterations and eight subsets (Flash 3D). Afterwards, the images were transferred into image processing software (Siemens Syngo.Via, Siemens Healthcare GmbH

Hybrid nanomaterials: from the laboratory to the market

• Verónica de Zea Bermudez,
• Fabrice Leroux,
• Pierre Rabu and
• Andreas Taubert

Beilstein J. Nanotechnol. 2017, 8, 861–862, doi:10.3762/bjnano.8.87

• fascinating materials into large-scale applications in fields that are relevant to our modern society. Examples are healthcare, energy, environment, safety and security, but also less obvious are fields like arts and restoration. This clearly shows that, beyond developing new hybrid materials, there is a need

Uptake of the proteins HTRA1 and HTRA2 by cells mediated by calcium phosphate nanoparticles

• Olga Rotan,
• Katharina N. Severin,
• Simon Pöpsel,
• Alexander Peetsch,
• Melisa Merdanovic,
• Michael Ehrmann and
• Matthias Epple

Beilstein J. Nanotechnol. 2017, 8, 381–393, doi:10.3762/bjnano.8.40

• containing HTRA2 were pooled and concentrated with protein concentrator spin columns (Vivaspin 20 MWCO 50,000, GE Healthcare Life Sciences). The concentrated protein was diluted with lysis buffer to a final imidazole concentration of 20 mM. The sample was then added to 2 mL Ni-NTA superflow resin (QIAGEN

Comparison of four functionalization methods of gold nanoparticles for enhancing the enzyme-linked immunosorbent assay (ELISA)

• Paula Ciaurriz,
• Fátima Fernández,
• Edurne Tellechea,
• Jose F. Moran and
• Aaron C. Asensio

Beilstein J. Nanotechnol. 2017, 8, 244–253, doi:10.3762/bjnano.8.27

• buffer exchanged against phosphate buffer 10 mM pH 7.4 using a Hi-Trap desalting column using an Äkta Prime apparatus (GE-Healthcare, Upsala, Sweden). The Ab-linker and HRP-linker concentrations were measured by absorption at 280 nm and 403 nm, respectively, as well as by Bradford assay (data not shown

Hemolysin coregulated protein 1 as a molecular gluing unit for the assembly of nanoparticle hybrid structures

• Tuan Anh Pham,
• Andreas Schreiber,
• Elena V. Sturm (née Rosseeva),
• Stefan Schiller and
• Helmut Cölfen

Beilstein J. Nanotechnol. 2016, 7, 351–363, doi:10.3762/bjnano.7.32

• % glycerol) of proteins (supplied by the Schreiber group) was removed with PD Spin Trap G-25 from GE Healthcare. The proteins were dissolved in water with 100 mM NaCl. Au NP Hcp1_cys3 network The Au NPs were used directly after synthesis. The NP concentration was determined by measuring the absorbance of the

Peptide-equipped tobacco mosaic virus templates for selective and controllable biomineral deposition

• Klara Altintoprak,
• Axel Seidenstücker,
• Alexander Welle,
• Sabine Eiben,
• Petia Atanasova,
• Nina Stitz,
• Alfred Plettl,
• Joachim Bill,
• Hartmut Gliemann,
• Holger Jeske,
• Dirk Rothenstein,
• Fania Geiger and
• Christina Wege

Beilstein J. Nanotechnol. 2015, 6, 1399–1412, doi:10.3762/bjnano.6.145

• example, as a structural modifier or filler in rubber [1], food [2][3] or healthcare products [4], bioceramics for medical purposes [5], mesoporous nanoparticulate or tubular drug delivery systems as reviewed in [6], sensor surfaces [7], or biocatalytic formulations as reviewed in [8]. An important focus

Functionalization of α-synuclein fibrils

• Simona Povilonienė,
• Vida Časaitė,
• Virginijus Bukauskas,
• Arūnas Šetkus,
• Juozas Staniulis and
• Rolandas Meškys

Beilstein J. Nanotechnol. 2015, 6, 124–133, doi:10.3762/bjnano.6.12

• ), containing 0.1 mM EDTA, 0.2 mM PMSF, and 500 mM NaCl. The cell lysate was heated at 100 °C for 10 min and subsequently centrifuged at 13200g for 20 min. The supernatant was dialyzed against 50 mM Tris-HCl, pH 8.0, 1 mM dithiothreitol, 1 mM EDTA, pH 8.0, and loaded into a HiTrap ANX column (GE Healthcare

• ) equilibrated in the same buffer. Proteins were eluted using a linear gradient of 0–1 M NaCl. Fractions containing α-SynC141 were pooled and additionally applied to a HiTrap Q XL column (GE Healthcare) and were identified by 15% SDS-PAGE. The α-Syn protein, expressed and purified in the same manner as α-SynC141

Coating with luminal gut-constituents alters adherence of nanoparticles to intestinal epithelial cells

• Heike Sinnecker,
• Katrin Ramaker and
• Andreas Frey

Beilstein J. Nanotechnol. 2014, 5, 2308–2315, doi:10.3762/bjnano.5.239

• . The biotinylated lectin RCA I was used in a concentration of 20 µg/mL in 0.2% gelatin/PBS to label the cells over night at 4 °C. Afterwards the cells were incubated with Cy5-conjugated streptavidin (GE Healthcare, Brand Amersham, Pittsburgh, USA), 5 µg/mL in 0.2% gelatin/PBS, for 90 min at rt. Finally

Effect of silver nanoparticles on human mesenchymal stem cell differentiation

• Christina Sengstock,
• Jörg Diendorf,
• Matthias Epple,
• Thomas A. Schildhauer and
• Manfred Köller

Beilstein J. Nanotechnol. 2014, 5, 2058–2069, doi:10.3762/bjnano.5.214

• (Ultraspec 3100 pro, Amersham Bioscience, GE Healthcare, Freiburg Germany) at a wavelength of 520 nm to measure the optical density of the extracted oil droplets. For Bodipy493/503 fluorescence quantification microphotographs were taken (Olympus CellP, Olympus, Hamburg, Germany) and digitally processed using

Silica nanoparticles are less toxic to human lung cells when deposited at the air–liquid interface compared to conventional submerged exposure

• Alicja Panas,
• Andreas Comouth,
• Harald Saathoff,
• Thomas Leisner,
• Marco Al-Rawi,
• Michael Simon,
• Gunnar Seemann,
• Olaf Dössel,
• Sonja Mülhopt,
• Hanns-Rudolf Paur,
• Susanne Fritsch-Decker,
• Carsten Weiss and
• Silvia Diabaté

Beilstein J. Nanotechnol. 2014, 5, 1590–1602, doi:10.3762/bjnano.5.171

• ) detection system from GE Healthcare (Freiburg, Germany) were used. Transwell® inserts containing polyester membranes of 24 mm diameter and with pores of 0.4 µm (Cat.No. 3450) were from Corning Life Sciences (Amsterdam, The Netherlands). 75-mesh formvar-coated copper grids for transmission electron